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The map label for this gene is prfC [H]

Identifier: 119717026

GI number: 119717026

Start: 2972690

End: 2974255

Strand: Reverse

Name: prfC [H]

Synonym: Noca_2802

Alternate gene names: 119717026

Gene position: 2974255-2972690 (Counterclockwise)

Preceding gene: 119717030

Following gene: 119717023

Centisome position: 59.65

GC content: 71.65

Gene sequence:

>1566_bases
GTGTCCGACACCCGCCCCCGCCGTACGTTCGCCGTCATCAGCCATCCCGACGCTGGCAAGTCCACGCTCACCGAGGCGCT
CGCCCTCCACGCGCGGGTGATCACCGAGGCGGGTGCCGTCCACGGCAAGGGGGAACGGCGGGCCACGGTCTCGGACTGGA
TGGCGATGGAGAAGGCGCGCGGCATCTCGATCACCTCGGCCGCGCTGCAGTTCGTGTTCCGCGACCACGTGATCAACCTC
GTCGACACCCCCGGCCACAGCGACTTCTCCGAGGACACCTACCGGGTGCTCTCCGCGGTCGACTCGGCGGTGATGCTGGT
CGACGCCGGCAAGGGCCTCGAGCCGCAGACGCTCAAGCTGTTCAAGGTCTGTGCGCTCCGCGGCATCCCGGTGATCACGG
TCATCAACAAGTGGGACCGTCCCGGCCTGTCGGCGCTGGGGCTGATGGACCTGATCCAGGAGCGGATCAAGCTCCGCCCC
ACGCCGCTCACCTGGCCCGTCGGCGAGGCCGGCGACTTCCGTGGCGTCGTGGACCGCCGCACCGGCGAGTTCGTGAAGTA
CACGCGCACGGCGGGCGGCGCCACCCGCGCGCCGGAGCGACGGTTGGCCGCGCACCAGGTCGAGGAGACCGACGCGCGCT
GCTGGGCGGCCGCGGTCGAGGAGCACGAGCTGCTCACCCTGGACGGCGCGGACCACGACCAGCAGAAGTTCCTGGCCGGC
GAGACCACGCCGGTGGTCTTCGCCTCGGCGCTGCAGAACTTCGGCGTCGCGCAGCTGCTGGAGCTGCTGCTCGACATCGC
CCCGGGCCCCGGGCCCGCGGAGGGGGTCGACGGTTCGGTGCGCCGGGTCACCGACGACTTCAGCGCGTTCGTCTTCAAGG
TCCAGTCGGGCATGAACAGCGCCCACCGCGACCGGCTCGCCTACGCCCGGGTCGTCTCGGGCACGTTCGAGCGCGGCATG
GTCGTGACCCACGCAGGCACCGGTCGTCCCTTCCCGACCAAGTTCGCGCAGTCGGTCTTCGGCCGCGAGACGACCTCGGT
CGAGACCGCCGAGGCCGGCGACATCATCGGCTTCGTCAACGCCCAGGCGCTGCGCGTCGGCGACACCGTGTACGTCGGGG
AGCGCGTCGAGTACCCACCCGTCCCGAGCTTCGCGCCGGAGCACTTCGTCACGGCGAGCGCGGGCGACATCGGCCGCTTC
AAGCAGTTCCGCCGGGGCATCGAGCAGCTGGACCAGGAGGGCGTCGTGCAGGTGCTGCGCTCGGACCTGCGCGGTGACCA
GAACCCGGTCCTGGCGGCCGTCGGACCGATGCAGTTCGAGGTCGTCGAGGACCGGATGACCAACGAGTTCAACGCGCCGA
TCCGGTTCTCCCGGCTCGACTACCAGGTCGCCCGACGCACCGATGCGGCGGGCGCCACGGCCCTGGCCGGGATGCGCGGC
GTCGAGGTGCTCGGGCGCAGCGACGGCACCCACCTGGCGCTGTTCGTCGACCAGTGGCGCGCGAACGTCACCGCCCGGGA
CAACCCGGACGTCCTGCTCGAGGCGCTGCCGGCCGGCGGGAGCTGA

Upstream 100 bases:

>100_bases
AAACCGGATTGATGCGATAGGCCATCGGTGCGAAACGGTGGAGTCCACCCGGACGCCAGCGAAGCGGGAGTACGCCGGTC
CAGCCGGTAGATTCCGGGCA

Downstream 100 bases:

>100_bases
CCTCGGTCCGGTCGCTAGAGCTTCGACATCTTGTGGAAGGGGCGCGGCACCCGGAGGGTCTGGGCGCCGAAGTCCACCAT
CACGGCGCTGGCGTCGACGT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSDTRPRRTFAVISHPDAGKSTLTEALALHARVITEAGAVHGKGERRATVSDWMAMEKARGISITSAALQFVFRDHVINL
VDTPGHSDFSEDTYRVLSAVDSAVMLVDAGKGLEPQTLKLFKVCALRGIPVITVINKWDRPGLSALGLMDLIQERIKLRP
TPLTWPVGEAGDFRGVVDRRTGEFVKYTRTAGGATRAPERRLAAHQVEETDARCWAAAVEEHELLTLDGADHDQQKFLAG
ETTPVVFASALQNFGVAQLLELLLDIAPGPGPAEGVDGSVRRVTDDFSAFVFKVQSGMNSAHRDRLAYARVVSGTFERGM
VVTHAGTGRPFPTKFAQSVFGRETTSVETAEAGDIIGFVNAQALRVGDTVYVGERVEYPPVPSFAPEHFVTASAGDIGRF
KQFRRGIEQLDQEGVVQVLRSDLRGDQNPVLAAVGPMQFEVVEDRMTNEFNAPIRFSRLDYQVARRTDAAGATALAGMRG
VEVLGRSDGTHLALFVDQWRANVTARDNPDVLLEALPAGGS

Sequences:

>Translated_521_residues
MSDTRPRRTFAVISHPDAGKSTLTEALALHARVITEAGAVHGKGERRATVSDWMAMEKARGISITSAALQFVFRDHVINL
VDTPGHSDFSEDTYRVLSAVDSAVMLVDAGKGLEPQTLKLFKVCALRGIPVITVINKWDRPGLSALGLMDLIQERIKLRP
TPLTWPVGEAGDFRGVVDRRTGEFVKYTRTAGGATRAPERRLAAHQVEETDARCWAAAVEEHELLTLDGADHDQQKFLAG
ETTPVVFASALQNFGVAQLLELLLDIAPGPGPAEGVDGSVRRVTDDFSAFVFKVQSGMNSAHRDRLAYARVVSGTFERGM
VVTHAGTGRPFPTKFAQSVFGRETTSVETAEAGDIIGFVNAQALRVGDTVYVGERVEYPPVPSFAPEHFVTASAGDIGRF
KQFRRGIEQLDQEGVVQVLRSDLRGDQNPVLAAVGPMQFEVVEDRMTNEFNAPIRFSRLDYQVARRTDAAGATALAGMRG
VEVLGRSDGTHLALFVDQWRANVTARDNPDVLLEALPAGGS
>Mature_520_residues
SDTRPRRTFAVISHPDAGKSTLTEALALHARVITEAGAVHGKGERRATVSDWMAMEKARGISITSAALQFVFRDHVINLV
DTPGHSDFSEDTYRVLSAVDSAVMLVDAGKGLEPQTLKLFKVCALRGIPVITVINKWDRPGLSALGLMDLIQERIKLRPT
PLTWPVGEAGDFRGVVDRRTGEFVKYTRTAGGATRAPERRLAAHQVEETDARCWAAAVEEHELLTLDGADHDQQKFLAGE
TTPVVFASALQNFGVAQLLELLLDIAPGPGPAEGVDGSVRRVTDDFSAFVFKVQSGMNSAHRDRLAYARVVSGTFERGMV
VTHAGTGRPFPTKFAQSVFGRETTSVETAEAGDIIGFVNAQALRVGDTVYVGERVEYPPVPSFAPEHFVTASAGDIGRFK
QFRRGIEQLDQEGVVQVLRSDLRGDQNPVLAAVGPMQFEVVEDRMTNEFNAPIRFSRLDYQVARRTDAAGATALAGMRGV
EVLGRSDGTHLALFVDQWRANVTARDNPDVLLEALPAGGS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=539, Percent_Identity=27.0871985157699, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI25306283, Length=390, Percent_Identity=29.4871794871795, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI18390331, Length=478, Percent_Identity=25.1046025104602, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI25306287, Length=290, Percent_Identity=32.4137931034483, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157426893, Length=136, Percent_Identity=33.8235294117647, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.1167883211679, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=33.5570469798658, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790835, Length=498, Percent_Identity=39.1566265060241, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1789738, Length=543, Percent_Identity=27.9926335174954, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI48994988, Length=420, Percent_Identity=26.4285714285714, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788922, Length=140, Percent_Identity=36.4285714285714, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=26.0330578512397, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17556745, Length=495, Percent_Identity=24.4444444444444, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=36.2962962962963, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17506493, Length=182, Percent_Identity=30.2197802197802, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=472, Percent_Identity=24.7881355932203, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322359, Length=523, Percent_Identity=26.0038240917782, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=35.5704697986577, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=35.5704697986577, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582462, Length=479, Percent_Identity=24.6346555323591, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI221458488, Length=565, Percent_Identity=22.1238938053097, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI78706572, Length=136, Percent_Identity=36.0294117647059, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585709, Length=160, Percent_Identity=33.75, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585711, Length=160, Percent_Identity=33.75, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585713, Length=160, Percent_Identity=33.75, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 56528; Mature: 56396

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTRPRRTFAVISHPDAGKSTLTEALALHARVITEAGAVHGKGERRATVSDWMAMEKAR
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
GISITSAALQFVFRDHVINLVDTPGHSDFSEDTYRVLSAVDSAVMLVDAGKGLEPQTLKL
CCCHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
FKVCALRGIPVITVINKWDRPGLSALGLMDLIQERIKLRPTPLTWPVGEAGDFRGVVDRR
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCC
TGEFVKYTRTAGGATRAPERRLAAHQVEETDARCWAAAVEEHELLTLDGADHDQQKFLAG
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCC
ETTPVVFASALQNFGVAQLLELLLDIAPGPGPAEGVDGSVRRVTDDFSAFVFKVQSGMNS
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
AHRDRLAYARVVSGTFERGMVVTHAGTGRPFPTKFAQSVFGRETTSVETAEAGDIIGFVN
HHHHHHHHHHHHHCHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECC
AQALRVGDTVYVGERVEYPPVPSFAPEHFVTASAGDIGRFKQFRRGIEQLDQEGVVQVLR
CCEEEECCEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
SDLRGDQNPVLAAVGPMQFEVVEDRMTNEFNAPIRFSRLDYQVARRTDAAGATALAGMRG
HHHCCCCCCEEEEECCEEHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHCCC
VEVLGRSDGTHLALFVDQWRANVTARDNPDVLLEALPAGGS
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
SDTRPRRTFAVISHPDAGKSTLTEALALHARVITEAGAVHGKGERRATVSDWMAMEKAR
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
GISITSAALQFVFRDHVINLVDTPGHSDFSEDTYRVLSAVDSAVMLVDAGKGLEPQTLKL
CCCHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHH
FKVCALRGIPVITVINKWDRPGLSALGLMDLIQERIKLRPTPLTWPVGEAGDFRGVVDRR
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCC
TGEFVKYTRTAGGATRAPERRLAAHQVEETDARCWAAAVEEHELLTLDGADHDQQKFLAG
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHCC
ETTPVVFASALQNFGVAQLLELLLDIAPGPGPAEGVDGSVRRVTDDFSAFVFKVQSGMNS
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
AHRDRLAYARVVSGTFERGMVVTHAGTGRPFPTKFAQSVFGRETTSVETAEAGDIIGFVN
HHHHHHHHHHHHHCHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECC
AQALRVGDTVYVGERVEYPPVPSFAPEHFVTASAGDIGRFKQFRRGIEQLDQEGVVQVLR
CCEEEECCEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHH
SDLRGDQNPVLAAVGPMQFEVVEDRMTNEFNAPIRFSRLDYQVARRTDAAGATALAGMRG
HHHCCCCCCEEEEECCEEHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHCCC
VEVLGRSDGTHLALFVDQWRANVTARDNPDVLLEALPAGGS
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA