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The map label for this gene is def [H]

Identifier: 119716668

GI number: 119716668

Start: 2603597

End: 2604142

Strand: Direct

Name: def [H]

Synonym: Noca_2441

Alternate gene names: 119716668

Gene position: 2603597-2604142 (Clockwise)

Preceding gene: 119716667

Following gene: 119716669

Centisome position: 52.22

GC content: 70.51

Gene sequence:

>546_bases
GTGGCCATCCAGCCGATCCGTCTCTTCGGGGACCCCGTCCTGCGCAAGCCCGCCATCGAGGTCGTCGACTTCGACAAGGA
GCTGCGCCGGCTCGTCGCGGACCTCACCGACACCATGATGGACGCCCCCGGCGCGGGGTTGGCCGCGCCGCAGATCGGCG
TGGGACTCCGGGTCTTCACCTGGTACGTCGACGGCGAGCCCGGGCACCTGGTCAACCCGCAGCTGGACCTGTCGGACGAG
CTGCAGGACGGTCCCGAGGGCTGTCTCTCGATCCCGGGCCTCAGCGTGGACTGCCAGCGCGCGATGGCTGCTGTGGCCCG
CGGCTTCAACATGTACGGCGACCCGGTGACCATCGAGGGCACCGAGCTGCTGGCCCGCGCGCTCCAGCACGAGACCGACC
ACCTCGACGGGGTGCTGTTCATCGACCGCCTGGACACCGAGGCCCGCAAGGCCGCGATGAAGGCGATCCGCGAGTCGGAG
TGGTTCGGCCTCGACGCCCCCCAGGTGAAGGTCTCTCCGCACGCCACGCACGGGCTCGGCTTCTGA

Upstream 100 bases:

>100_bases
CCAGGTCGACCCACCGACCCTCTGACCGACGGCACGGCCCCGGGTGCTCGCTCGACCACCACCAGGGAGTCCGCTGACGC
GTCCTCCCTAGACTGACGCC

Downstream 100 bases:

>100_bases
GCATGCGCGTCGTCTTCGCCGGCACCCCCGAGGTGGCCATCCCCGTCCTGGACGCGGTCGCCGCGTCCTCCCACGAGCTC
GTCGCCGTGGTCACCCGTCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MAIQPIRLFGDPVLRKPAIEVVDFDKELRRLVADLTDTMMDAPGAGLAAPQIGVGLRVFTWYVDGEPGHLVNPQLDLSDE
LQDGPEGCLSIPGLSVDCQRAMAAVARGFNMYGDPVTIEGTELLARALQHETDHLDGVLFIDRLDTEARKAAMKAIRESE
WFGLDAPQVKVSPHATHGLGF

Sequences:

>Translated_181_residues
MAIQPIRLFGDPVLRKPAIEVVDFDKELRRLVADLTDTMMDAPGAGLAAPQIGVGLRVFTWYVDGEPGHLVNPQLDLSDE
LQDGPEGCLSIPGLSVDCQRAMAAVARGFNMYGDPVTIEGTELLARALQHETDHLDGVLFIDRLDTEARKAAMKAIRESE
WFGLDAPQVKVSPHATHGLGF
>Mature_180_residues
AIQPIRLFGDPVLRKPAIEVVDFDKELRRLVADLTDTMMDAPGAGLAAPQIGVGLRVFTWYVDGEPGHLVNPQLDLSDEL
QDGPEGCLSIPGLSVDCQRAMAAVARGFNMYGDPVTIEGTELLARALQHETDHLDGVLFIDRLDTEARKAAMKAIRESEW
FGLDAPQVKVSPHATHGLGF

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=161, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789682, Length=148, Percent_Identity=37.1621621621622, Blast_Score=77, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24645726, Length=160, Percent_Identity=28.75, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19687; Mature: 19556

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQPIRLFGDPVLRKPAIEVVDFDKELRRLVADLTDTMMDAPGAGLAAPQIGVGLRVFT
CCCCCCHHHCCCHHCCCCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
WYVDGEPGHLVNPQLDLSDELQDGPEGCLSIPGLSVDCQRAMAAVARGFNMYGDPVTIEG
EEEECCCCCCCCCCCCCCHHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCEEECH
TELLARALQHETDHLDGVLFIDRLDTEARKAAMKAIRESEWFGLDAPQVKVSPHATHGLG
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
F
C
>Mature Secondary Structure 
AIQPIRLFGDPVLRKPAIEVVDFDKELRRLVADLTDTMMDAPGAGLAAPQIGVGLRVFT
CCCCCHHHCCCHHCCCCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
WYVDGEPGHLVNPQLDLSDELQDGPEGCLSIPGLSVDCQRAMAAVARGFNMYGDPVTIEG
EEEECCCCCCCCCCCCCCHHHCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCEEECH
TELLARALQHETDHLDGVLFIDRLDTEARKAAMKAIRESEWFGLDAPQVKVSPHATHGLG
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA