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The map label for this gene is priA [H]

Identifier: 119716667

GI number: 119716667

Start: 2601488

End: 2603521

Strand: Direct

Name: priA [H]

Synonym: Noca_2440

Alternate gene names: 119716667

Gene position: 2601488-2603521 (Clockwise)

Preceding gene: 119716666

Following gene: 119716668

Centisome position: 52.18

GC content: 77.88

Gene sequence:

>2034_bases
ATGACGGACTCGGAGGAGCAGCCCGAGCTGCTGCCGGGGATGGTCCGGGCGAGCGTCAAGGCGTCGCGTGCCAAGGCGCG
GGCGACCCGGGCCCGCAAGGCCGCCGAGGCCGAGCCGGCCGCGGTCGACCCGGTCGCCCGGGTGCTGGTCGACCTCCCGC
TCGCCCACCTGGACCGGCCCTTCGACTACGCCGTCCCGGCCGCGATGGCCGACGACGCCCGGCCCGGAGCGCGGGTGAAG
GTCCGCTTCGCGGGTCAGGACGTCGACGGCTACCTCGTGGCCCGGGCGGCGGAGTCCGAGCACCTCGGCCGGCTCGCGCC
CCTGCGGCGTGTGGTGAGCCCCGAGCCGGTGCTGGCGCCCCAGATCGAGGCGCTCAGCGCCGAGCTCGCGGAGCGGTACG
CCGGCACCCGCTCCGACGTGCTCCGGCTGGCGGTCCCGCCCCGGCACGCGACCACCGAGAAGGCGCCCAGTCCGCCGGTG
CCGTCCTGGGGCGGACCCGACCCGGCCGCGGGCGAGGCGGCCTGGGCCGGACACGAGCCGGGCGCGGCCTTCCTGCGGCA
CCTGACCGCCGGGGGCATCCCGCGCGCGGTGTGGACGGCCGCCCCGGCGGCCGACTGGCCCGTGATGATCGCCCACGCCG
TGGCAGCGACGTACGCCTCCGGGCGCGGGAGCCTGGTCTGCGTCCCGGACCACAAGGACGTCGCCCGGGTCGGTGCGGCG
CTCACCGACGTCCTCGGCGAGGGCCGGCACGTCACCCTGACCGCGGACTCCGGGCCGGCGGCGCGCTACCGCGACTTCCT
CGCCGTCAGCCGGGGCGCGCGTCGGGTGGTCGTCGGCACCCGTGGGGCGGCGTTCGCCCCGGTCCACGAGCTCGGGCTGG
TCGTGGTGTGGGACGACGGTGACGACCTGCACGCGGAGCCGCGCGCGCCCTACCCGCACACGCGCGAGACCCTGCTCCTG
CGGGCCGAGCGCGAGGGTGCCGCTGCACTCGTCGGTGGCTTCGTGCGCACCGTGGAGGGCGAGTACCTCCTCCGCACCGG
CTGGGCCCACGAGCTGGTGCCCGCACGCGAGGAGGTGCGCCGACGGGTCACGGTGTCGGTGGCCGGGGACGCCGGTGACC
GCGACGTGCACGCGCGCGGGGTGCGGATGCCCCGCGCGATCCACCAGGCGATCCGGGAGGCACTGGACGACGGGCCCGTC
CTCGTGCAGACGCCGCGGGTGGGCTACGTCAACGCCCTGGCCTGCGAGCGCTGCCGGACGCCGGCCCGTTGTGCTGCCTG
CCAGGGACCGCTCGCCCTGACCGGCCCGACGACCCCGCCCGCCTGCCGCTGGTGCGGGATCCTGGCGGAGGGCTGGGCGT
GCCGGGAGTGTGGCTTCCGGGGGCTGCGTGCTCCCGTCCTCGGCGATGCCCGTACGGCCGAGGAGCTGGGACGGGCCTTC
CCCGGCACCACCGTGCGCACCAGCTCGGGCGAGGCCGTCGTGGCAGCGGTGGGCGCCCGTCCCCAGGTCGTCGTGGCGAC
TCCCGGCGCCGAGCCGGTCGCCGAGGACGGCTACGCCGCGGTGGTGCTGCTCGACACCTGGCTGCTGCTGTCCCGGATCG
ACCTGCGCGCCGAGGAGGAGGCGCTGCGCCGGTGGGCCAACGCCGCAGGCCTGGTGCGCCCCGGCGGCCGGGTCGTGGCC
GTCGGCGACGCGGCCCACCCGGCGCTCCAGGCGCTCGTGCGCTGGGACCCGGCCGGGTTCGCCGCCCGGGAGACCGCCGA
GCGGGTCGAGGCGCACCTGCCGCCGGCCAGCCGGCTCGCGACGATCACCGGGGAGCCGGGGGCGGTCGACGACGCCTTGA
TCCTCCTGGACGCCCCATCGGGTGCCGAGATCCTCGGCCCGGTGGACGTCGGGGAGGGCGAGTCCCGCGTCGTCGTCCGG
GTGCCACGCGCGCAGGGGCCGGCGCTCTCCCACGCGCTCGGCCAGCTCCAGCGGGTCCGCTCGAGCCGCAAGCTCGACGC
CGTCCGCATCCAGGTCGACCCACCGACCCTCTGA

Upstream 100 bases:

>100_bases
CGCCGCGCGGGTCGCGGGGTCGGTCCTCGGGCCGGCCAGTCCTCGTTGTGGTTACGGTGGGTGCGCGGGTGCGTCGCCGC
CCCCTGGTAGACATCAGCCC

Downstream 100 bases:

>100_bases
CCGACGGCACGGCCCCGGGTGCTCGCTCGACCACCACCAGGGAGTCCGCTGACGCGTCCTCCCTAGACTGACGCCGTGGC
CATCCAGCCGATCCGTCTCT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MTDSEEQPELLPGMVRASVKASRAKARATRARKAAEAEPAAVDPVARVLVDLPLAHLDRPFDYAVPAAMADDARPGARVK
VRFAGQDVDGYLVARAAESEHLGRLAPLRRVVSPEPVLAPQIEALSAELAERYAGTRSDVLRLAVPPRHATTEKAPSPPV
PSWGGPDPAAGEAAWAGHEPGAAFLRHLTAGGIPRAVWTAAPAADWPVMIAHAVAATYASGRGSLVCVPDHKDVARVGAA
LTDVLGEGRHVTLTADSGPAARYRDFLAVSRGARRVVVGTRGAAFAPVHELGLVVVWDDGDDLHAEPRAPYPHTRETLLL
RAEREGAAALVGGFVRTVEGEYLLRTGWAHELVPAREEVRRRVTVSVAGDAGDRDVHARGVRMPRAIHQAIREALDDGPV
LVQTPRVGYVNALACERCRTPARCAACQGPLALTGPTTPPACRWCGILAEGWACRECGFRGLRAPVLGDARTAEELGRAF
PGTTVRTSSGEAVVAAVGARPQVVVATPGAEPVAEDGYAAVVLLDTWLLLSRIDLRAEEEALRRWANAAGLVRPGGRVVA
VGDAAHPALQALVRWDPAGFAARETAERVEAHLPPASRLATITGEPGAVDDALILLDAPSGAEILGPVDVGEGESRVVVR
VPRAQGPALSHALGQLQRVRSSRKLDAVRIQVDPPTL

Sequences:

>Translated_677_residues
MTDSEEQPELLPGMVRASVKASRAKARATRARKAAEAEPAAVDPVARVLVDLPLAHLDRPFDYAVPAAMADDARPGARVK
VRFAGQDVDGYLVARAAESEHLGRLAPLRRVVSPEPVLAPQIEALSAELAERYAGTRSDVLRLAVPPRHATTEKAPSPPV
PSWGGPDPAAGEAAWAGHEPGAAFLRHLTAGGIPRAVWTAAPAADWPVMIAHAVAATYASGRGSLVCVPDHKDVARVGAA
LTDVLGEGRHVTLTADSGPAARYRDFLAVSRGARRVVVGTRGAAFAPVHELGLVVVWDDGDDLHAEPRAPYPHTRETLLL
RAEREGAAALVGGFVRTVEGEYLLRTGWAHELVPAREEVRRRVTVSVAGDAGDRDVHARGVRMPRAIHQAIREALDDGPV
LVQTPRVGYVNALACERCRTPARCAACQGPLALTGPTTPPACRWCGILAEGWACRECGFRGLRAPVLGDARTAEELGRAF
PGTTVRTSSGEAVVAAVGARPQVVVATPGAEPVAEDGYAAVVLLDTWLLLSRIDLRAEEEALRRWANAAGLVRPGGRVVA
VGDAAHPALQALVRWDPAGFAARETAERVEAHLPPASRLATITGEPGAVDDALILLDAPSGAEILGPVDVGEGESRVVVR
VPRAQGPALSHALGQLQRVRSSRKLDAVRIQVDPPTL
>Mature_676_residues
TDSEEQPELLPGMVRASVKASRAKARATRARKAAEAEPAAVDPVARVLVDLPLAHLDRPFDYAVPAAMADDARPGARVKV
RFAGQDVDGYLVARAAESEHLGRLAPLRRVVSPEPVLAPQIEALSAELAERYAGTRSDVLRLAVPPRHATTEKAPSPPVP
SWGGPDPAAGEAAWAGHEPGAAFLRHLTAGGIPRAVWTAAPAADWPVMIAHAVAATYASGRGSLVCVPDHKDVARVGAAL
TDVLGEGRHVTLTADSGPAARYRDFLAVSRGARRVVVGTRGAAFAPVHELGLVVVWDDGDDLHAEPRAPYPHTRETLLLR
AEREGAAALVGGFVRTVEGEYLLRTGWAHELVPAREEVRRRVTVSVAGDAGDRDVHARGVRMPRAIHQAIREALDDGPVL
VQTPRVGYVNALACERCRTPARCAACQGPLALTGPTTPPACRWCGILAEGWACRECGFRGLRAPVLGDARTAEELGRAFP
GTTVRTSSGEAVVAAVGARPQVVVATPGAEPVAEDGYAAVVLLDTWLLLSRIDLRAEEEALRRWANAAGLVRPGGRVVAV
GDAAHPALQALVRWDPAGFAARETAERVEAHLPPASRLATITGEPGAVDDALILLDAPSGAEILGPVDVGEGESRVVVRV
PRAQGPALSHALGQLQRVRSSRKLDAVRIQVDPPTL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=443, Percent_Identity=24.3792325056433, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 71362; Mature: 71231

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSEEQPELLPGMVRASVKASRAKARATRARKAAEAEPAAVDPVARVLVDLPLAHLDRP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCC
FDYAVPAAMADDARPGARVKVRFAGQDVDGYLVARAAESEHLGRLAPLRRVVSPEPVLAP
CCCCCCCHHCCCCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCC
QIEALSAELAERYAGTRSDVLRLAVPPRHATTEKAPSPPVPSWGGPDPAAGEAAWAGHEP
HHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAAFLRHLTAGGIPRAVWTAAPAADWPVMIAHAVAATYASGRGSLVCVPDHKDVARVGAA
HHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
LTDVLGEGRHVTLTADSGPAARYRDFLAVSRGARRVVVGTRGAAFAPVHELGLVVVWDDG
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCEEEEECCC
DDLHAEPRAPYPHTRETLLLRAEREGAAALVGGFVRTVEGEYLLRTGWAHELVPAREEVR
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHCCCHHHHC
RRVTVSVAGDAGDRDVHARGVRMPRAIHQAIREALDDGPVLVQTPRVGYVNALACERCRT
CEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCC
PARCAACQGPLALTGPTTPPACRWCGILAEGWACRECGFRGLRAPVLGDARTAEELGRAF
CHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCC
PGTTVRTSSGEAVVAAVGARPQVVVATPGAEPVAEDGYAAVVLLDTWLLLSRIDLRAEEE
CCCEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCHHH
ALRRWANAAGLVRPGGRVVAVGDAAHPALQALVRWDPAGFAARETAERVEAHLPPASRLA
HHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHEE
TITGEPGAVDDALILLDAPSGAEILGPVDVGEGESRVVVRVPRAQGPALSHALGQLQRVR
EECCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
SSRKLDAVRIQVDPPTL
HCCCEEEEEEEECCCCC
>Mature Secondary Structure 
TDSEEQPELLPGMVRASVKASRAKARATRARKAAEAEPAAVDPVARVLVDLPLAHLDRP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCC
FDYAVPAAMADDARPGARVKVRFAGQDVDGYLVARAAESEHLGRLAPLRRVVSPEPVLAP
CCCCCCCHHCCCCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCC
QIEALSAELAERYAGTRSDVLRLAVPPRHATTEKAPSPPVPSWGGPDPAAGEAAWAGHEP
HHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAAFLRHLTAGGIPRAVWTAAPAADWPVMIAHAVAATYASGRGSLVCVPDHKDVARVGAA
HHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
LTDVLGEGRHVTLTADSGPAARYRDFLAVSRGARRVVVGTRGAAFAPVHELGLVVVWDDG
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCEEEEECCC
DDLHAEPRAPYPHTRETLLLRAEREGAAALVGGFVRTVEGEYLLRTGWAHELVPAREEVR
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHCCCHHHHC
RRVTVSVAGDAGDRDVHARGVRMPRAIHQAIREALDDGPVLVQTPRVGYVNALACERCRT
CEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCC
PARCAACQGPLALTGPTTPPACRWCGILAEGWACRECGFRGLRAPVLGDARTAEELGRAF
CHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCC
PGTTVRTSSGEAVVAAVGARPQVVVATPGAEPVAEDGYAAVVLLDTWLLLSRIDLRAEEE
CCCEEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCHHH
ALRRWANAAGLVRPGGRVVAVGDAAHPALQALVRWDPAGFAARETAERVEAHLPPASRLA
HHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHEE
TITGEPGAVDDALILLDAPSGAEILGPVDVGEGESRVVVRVPRAQGPALSHALGQLQRVR
EECCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
SSRKLDAVRIQVDPPTL
HCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]