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The map label for this gene is rimO

Identifier: 119716546

GI number: 119716546

Start: 2471669

End: 2473111

Strand: Direct

Name: rimO

Synonym: Noca_2319

Alternate gene names: 119716546

Gene position: 2471669-2473111 (Clockwise)

Preceding gene: 119716545

Following gene: 119716547

Centisome position: 49.57

GC content: 74.29

Gene sequence:

>1443_bases
ATGACGACTGACTCCAGCCCTGCCCCCGTCCGCGAACTGCTGTCGGTGGCGATGGTGACCCTCGGCTGCGCCCGCAACGA
GGTCGATTCCGAGGAGCTCGCCGGGCGCCTCGAGGCCGACGGGTTCCGGCTGGTCCAGGACCCGGCGGAGGCCGACACGG
TCGTCGTCAACACGTGCGGCTTCGTCGAGGCCGCGAAGAAGGACTCCGTCGACACCCTGCTCCAGGCGGCCGACCTCAAG
GCCCGGTCCGAGCAGGGGCAGGGCCGCACGCGGGCCGTGGTCGCCGTCGGCTGCCTGGCCGAGCGCTACGGCAAGGACCT
CGCCGCCTCCCTGCCCGAGGCCGACGCCGTCTTCGGCTTCGACGACTACCCCGACATCGCCGCCCGACTGCGCTCGATCG
TCGCGGGCGAGGCCCACGAGGCGCACACCCCCCACGACCGCCGCACGCTGCTGCCGATCACCCCGGTGGAGCGGGTCCTG
TCCACCACGCCGGTGCCCGGCCACGCCTCCGGCCCGGCGTCGGTACGACGGCGCCTCGACGACGGCCCGACGGCGGCGCT
GAAGCTGGCCAGCGGGTGCGACCGGCGCTGCTCGTTCTGTGCGATCCCGGCCTTCCGGGGCTCGTTCCTGAGCCGCCGCC
CGAGCGACGTGCTCCAGGAGGCGCGCTGGCTCGCCGACCACGGCGCTCGCGAGCTGTTCCTGGTCAGCGAGAACTCGACC
TCCTACGGCAAGGACCTGGGCGACCTGCGGCTGCTCGAGACGCTGCTGCCCGAGCTGGCCGCCGTGGACGGGGTGGAGCG
GGTCCGCGTCTCCTACCTGCAGCCGGCCGAGACCCGGGCGGGACTGATCGAGGCCATCGCGAGCACGCCCGGGGTGGCGC
CGTACTTCGACCTGTCCTTCCAGCACGCGAGCGCCCCGGTGCTGCGCCGGATGCGCCGGTTCGGCGACCCGGAGAGCTTC
CTCGCCCTGCTCGCGCAGGTCCGGGGCCACGCGCCGGCGGCGGGCGTGCGCAGCAACGTGATCGTGGGCTTCCCCGGCGA
GACCGAGCAGGACCTCGAGACCCTGTGCGACTTCCTGGTCGCCGCGCGCCTCGACGTCACCGGCGTGTTCGGCTACTCCG
ACGAGGACGGCACCGAGGCCGCGACGTACGACGGCAAGCTCGACGACGACGAGATCCGAGCCCGGGTCGAGCACGTGAGC
GACCTGGTCGAGGAGCTGACCTCCCAGCGGGCGGCCGAGCGGCTCGGCGAGCAGGTCGAGGTGCTCGTGGAACGGGTCGA
GGACGGCCCGGGCGGGCGCACCGTCGAGGGCCGGGCGGCCCACCAGGGCCCCGAGGTCGACGGCGCCACCTACCTGCTGG
ACAGCGACGCCCGCGTCGGCGACCTGGTGCGTGCGGTCGTGGTGGGCTCCGAGGGCGCCGACCTGGACGCGAGACCCCTG
TGA

Upstream 100 bases:

>100_bases
CGGGCAGCCCGCGCAGAACACCTTCGCGGCCGCGACCGAGTGACGCCGCCGCGGCCCGTTTCGACGGGCCGCGGCCCGGT
TGACATACTCGACGACGACC

Downstream 100 bases:

>100_bases
CGACCGAGGGTGCTGTGATGACCGAGGTGCCGCGATGACCGAGGTGGCGGCCAAGCCGAGCAACTGGAACGTTCCGAACG
TGCTCACCACGTTGCGGATC

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTTDSSPAPVRELLSVAMVTLGCARNEVDSEELAGRLEADGFRLVQDPAEADTVVVNTCGFVEAAKKDSVDTLLQAADLK
ARSEQGQGRTRAVVAVGCLAERYGKDLAASLPEADAVFGFDDYPDIAARLRSIVAGEAHEAHTPHDRRTLLPITPVERVL
STTPVPGHASGPASVRRRLDDGPTAALKLASGCDRRCSFCAIPAFRGSFLSRRPSDVLQEARWLADHGARELFLVSENST
SYGKDLGDLRLLETLLPELAAVDGVERVRVSYLQPAETRAGLIEAIASTPGVAPYFDLSFQHASAPVLRRMRRFGDPESF
LALLAQVRGHAPAAGVRSNVIVGFPGETEQDLETLCDFLVAARLDVTGVFGYSDEDGTEAATYDGKLDDDEIRARVEHVS
DLVEELTSQRAAERLGEQVEVLVERVEDGPGGRTVEGRAAHQGPEVDGATYLLDSDARVGDLVRAVVVGSEGADLDARPL

Sequences:

>Translated_480_residues
MTTDSSPAPVRELLSVAMVTLGCARNEVDSEELAGRLEADGFRLVQDPAEADTVVVNTCGFVEAAKKDSVDTLLQAADLK
ARSEQGQGRTRAVVAVGCLAERYGKDLAASLPEADAVFGFDDYPDIAARLRSIVAGEAHEAHTPHDRRTLLPITPVERVL
STTPVPGHASGPASVRRRLDDGPTAALKLASGCDRRCSFCAIPAFRGSFLSRRPSDVLQEARWLADHGARELFLVSENST
SYGKDLGDLRLLETLLPELAAVDGVERVRVSYLQPAETRAGLIEAIASTPGVAPYFDLSFQHASAPVLRRMRRFGDPESF
LALLAQVRGHAPAAGVRSNVIVGFPGETEQDLETLCDFLVAARLDVTGVFGYSDEDGTEAATYDGKLDDDEIRARVEHVS
DLVEELTSQRAAERLGEQVEVLVERVEDGPGGRTVEGRAAHQGPEVDGATYLLDSDARVGDLVRAVVVGSEGADLDARPL
>Mature_479_residues
TTDSSPAPVRELLSVAMVTLGCARNEVDSEELAGRLEADGFRLVQDPAEADTVVVNTCGFVEAAKKDSVDTLLQAADLKA
RSEQGQGRTRAVVAVGCLAERYGKDLAASLPEADAVFGFDDYPDIAARLRSIVAGEAHEAHTPHDRRTLLPITPVERVLS
TTPVPGHASGPASVRRRLDDGPTAALKLASGCDRRCSFCAIPAFRGSFLSRRPSDVLQEARWLADHGARELFLVSENSTS
YGKDLGDLRLLETLLPELAAVDGVERVRVSYLQPAETRAGLIEAIASTPGVAPYFDLSFQHASAPVLRRMRRFGDPESFL
ALLAQVRGHAPAAGVRSNVIVGFPGETEQDLETLCDFLVAARLDVTGVFGYSDEDGTEAATYDGKLDDDEIRARVEHVSD
LVEELTSQRAAERLGEQVEVLVERVEDGPGGRTVEGRAAHQGPEVDGATYLLDSDARVGDLVRAVVVGSEGADLDARPL

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=437, Percent_Identity=25.858123569794, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI28872784, Length=438, Percent_Identity=25.7990867579909, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI93277076, Length=419, Percent_Identity=22.1957040572792, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1787057, Length=471, Percent_Identity=30.1486199575372, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1786882, Length=410, Percent_Identity=27.5609756097561, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=412, Percent_Identity=25.9708737864078, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71996771, Length=246, Percent_Identity=29.6747967479675, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356207, Length=439, Percent_Identity=25.7403189066059, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922432, Length=423, Percent_Identity=25.531914893617, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_NOCSJ (A1SJ39)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_923511.1
- ProteinModelPortal:   A1SJ39
- SMR:   A1SJ39
- STRING:   A1SJ39
- GeneID:   4595934
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_2319
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   A1SJ39
- ProtClustDB:   CLSK902972
- BioCyc:   NSP35761:NOCA_2319-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51390; Mature: 51259

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDSSPAPVRELLSVAMVTLGCARNEVDSEELAGRLEADGFRLVQDPAEADTVVVNTCG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCCCCCEEEEECCC
FVEAAKKDSVDTLLQAADLKARSEQGQGRTRAVVAVGCLAERYGKDLAASLPEADAVFGF
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCEECCC
DDYPDIAARLRSIVAGEAHEAHTPHDRRTLLPITPVERVLSTTPVPGHASGPASVRRRLD
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCCCCCCHHHHHHHCC
DGPTAALKLASGCDRRCSFCAIPAFRGSFLSRRPSDVLQEARWLADHGARELFLVSENST
CCCHHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCC
SYGKDLGDLRLLETLLPELAAVDGVERVRVSYLQPAETRAGLIEAIASTPGVAPYFDLSF
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEECCCC
QHASAPVLRRMRRFGDPESFLALLAQVRGHAPAAGVRSNVIVGFPGETEQDLETLCDFLV
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
AARLDVTGVFGYSDEDGTEAATYDGKLDDDEIRARVEHVSDLVEELTSQRAAERLGEQVE
HHHHCCEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVERVEDGPGGRTVEGRAAHQGPEVDGATYLLDSDARVGDLVRAVVVGSEGADLDARPL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TTDSSPAPVRELLSVAMVTLGCARNEVDSEELAGRLEADGFRLVQDPAEADTVVVNTCG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCCCCCEEEEECCC
FVEAAKKDSVDTLLQAADLKARSEQGQGRTRAVVAVGCLAERYGKDLAASLPEADAVFGF
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCEECCC
DDYPDIAARLRSIVAGEAHEAHTPHDRRTLLPITPVERVLSTTPVPGHASGPASVRRRLD
CCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCCCCCCHHHHHHHCC
DGPTAALKLASGCDRRCSFCAIPAFRGSFLSRRPSDVLQEARWLADHGARELFLVSENST
CCCHHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCC
SYGKDLGDLRLLETLLPELAAVDGVERVRVSYLQPAETRAGLIEAIASTPGVAPYFDLSF
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEECCCC
QHASAPVLRRMRRFGDPESFLALLAQVRGHAPAAGVRSNVIVGFPGETEQDLETLCDFLV
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
AARLDVTGVFGYSDEDGTEAATYDGKLDDDEIRARVEHVSDLVEELTSQRAAERLGEQVE
HHHHCCEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVERVEDGPGGRTVEGRAAHQGPEVDGATYLLDSDARVGDLVRAVVVGSEGADLDARPL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA