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The map label for this gene is cbaA [H]

Identifier: 119716368

GI number: 119716368

Start: 2285841

End: 2287193

Strand: Reverse

Name: cbaA [H]

Synonym: Noca_2137

Alternate gene names: 119716368

Gene position: 2287193-2285841 (Counterclockwise)

Preceding gene: 119716369

Following gene: 119716367

Centisome position: 45.87

GC content: 63.19

Gene sequence:

>1353_bases
ATGGCAGGTAGCGCAGGAGCGCGGCCCGCAGCCGCGGAGGACGAGAACACCAACTCTCTTCACCCCTCAGCACTGCGCGA
GCGAATTCCGGAGATGGGACTTAAGGAGTACTGGTACCCCGCGATCGAGGCAAAGAAGGTCAAGTCCGGCAAGCCGACCA
CGGTCAAGATGCTTGGGCAGGACCTCTCGTTCTTTCGTGGCAAGGACGGGAACGTGGTGGCCACCAGCAGTACGTGCCCA
CACCGCGGAGCCAGCCTCGCGCACGGCAAGTGCCACTTCGCGGGCACGATCACGTGTCCCTACCACGGTTGGACCTTCGA
CGAGACCGGCGCGTGTGTGGCGGTACTGGGCGAAGGCCCCGAGTCGCGCATCCCCGGAATGCCTGACGCCCGGGTCCGTT
CGTACCCGACCCAGACCCACAAGGGCATCGTCTTCGCATGGATGGGCGAGAGCGACCCAGCGCCGATTGAAGAGGATGTG
CCGCCGCAGTTCTTCAACGACGAATTTCTCATCCAGGTCAGCGTGACCAGTTGGAACTGCAACTGGCGGCCGGCCTTCGA
GAACCTTCTCGACTCGCACGTCTTTTACGTGCACCGGAACTCCGTGCACCTCATGATGATGGATGCAGCGAACCTCTTGA
TCGCCAGCAAGATGGGCCCCCGTCGGCCCCGTCCGCGCGTCGTCAACGGACGCGCACTCATGTACCCGCCTGGCGCCCTG
GGGTTCGCCGACGCTTTCGTCGGGACCGGCGCCAAGCACGCCGCCGAGAAGGCGACAGCGACCGAGGTTAAGACCTGGCC
CCCTGCCCCGGAGGCAGGCCTGCAGGACTCCTACCCCGGCCTCGGCGGAGCCCAGTGGCCGAAGAGTGATCGGCGGGTCG
CGTGGCACAAGGTCTACGGCGCGACGAAGAAGGTCATGCCCACGCCCTTTAAGCGCGACCCACTTGAGATCGACCCTGAG
TGGAAGGATGCACACCTTCCGTGCACATTCCAGGTCTCGTACTCCGATCACATCTACTCCCGCATCACCGTTCCGATTGA
TGCCGAAACGTCTCGAGTGTTCTACTTCCACGCCACGATGCCGCTGACCCGGACCAAGCGAATCTGGGACCACGTGTACT
TCGAGGTCTTCCAGAACTGGATGATGAACTACAACTTCTCCGGCCAGGACGAGAAGGTCGTGCGCCATCAGGCCTACGAC
ACCCCGGAGACGTTCTCGGCCACCGATGTCTTCCCGCTCACCCTGCGCAAGCTCATTCTCGAAAACGCACGCGACTTCCA
CCTCAAGGACGGTCACGACACCGAGTGGCCTGATGCTGCAGGCGCCACGTCGGCCAAGGAGGACAGCAAATGA

Upstream 100 bases:

>100_bases
GCCTTCGCCGATATCCCCGATAAGTCCCGTCAGATCGACCGGGCTTGGGCCTACTACTGCACCCGGCAAGACGTCGGTGC
CCATCAGAAGGGACAGAACA

Downstream 100 bases:

>100_bases
GGCCTGAAGACGCCGACCTAGATGTTCGGGCCGGCAATCGACTGCGCGGCAAAACCGTCATCATCACCGGTGCCGGGCGC
GGGATCGGACGGGCAGCCGC

Product: Rieske (2Fe-2S) domain-containing protein

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAGSAGARPAAAEDENTNSLHPSALRERIPEMGLKEYWYPAIEAKKVKSGKPTTVKMLGQDLSFFRGKDGNVVATSSTCP
HRGASLAHGKCHFAGTITCPYHGWTFDETGACVAVLGEGPESRIPGMPDARVRSYPTQTHKGIVFAWMGESDPAPIEEDV
PPQFFNDEFLIQVSVTSWNCNWRPAFENLLDSHVFYVHRNSVHLMMMDAANLLIASKMGPRRPRPRVVNGRALMYPPGAL
GFADAFVGTGAKHAAEKATATEVKTWPPAPEAGLQDSYPGLGGAQWPKSDRRVAWHKVYGATKKVMPTPFKRDPLEIDPE
WKDAHLPCTFQVSYSDHIYSRITVPIDAETSRVFYFHATMPLTRTKRIWDHVYFEVFQNWMMNYNFSGQDEKVVRHQAYD
TPETFSATDVFPLTLRKLILENARDFHLKDGHDTEWPDAAGATSAKEDSK

Sequences:

>Translated_450_residues
MAGSAGARPAAAEDENTNSLHPSALRERIPEMGLKEYWYPAIEAKKVKSGKPTTVKMLGQDLSFFRGKDGNVVATSSTCP
HRGASLAHGKCHFAGTITCPYHGWTFDETGACVAVLGEGPESRIPGMPDARVRSYPTQTHKGIVFAWMGESDPAPIEEDV
PPQFFNDEFLIQVSVTSWNCNWRPAFENLLDSHVFYVHRNSVHLMMMDAANLLIASKMGPRRPRPRVVNGRALMYPPGAL
GFADAFVGTGAKHAAEKATATEVKTWPPAPEAGLQDSYPGLGGAQWPKSDRRVAWHKVYGATKKVMPTPFKRDPLEIDPE
WKDAHLPCTFQVSYSDHIYSRITVPIDAETSRVFYFHATMPLTRTKRIWDHVYFEVFQNWMMNYNFSGQDEKVVRHQAYD
TPETFSATDVFPLTLRKLILENARDFHLKDGHDTEWPDAAGATSAKEDSK
>Mature_449_residues
AGSAGARPAAAEDENTNSLHPSALRERIPEMGLKEYWYPAIEAKKVKSGKPTTVKMLGQDLSFFRGKDGNVVATSSTCPH
RGASLAHGKCHFAGTITCPYHGWTFDETGACVAVLGEGPESRIPGMPDARVRSYPTQTHKGIVFAWMGESDPAPIEEDVP
PQFFNDEFLIQVSVTSWNCNWRPAFENLLDSHVFYVHRNSVHLMMMDAANLLIASKMGPRRPRPRVVNGRALMYPPGALG
FADAFVGTGAKHAAEKATATEVKTWPPAPEAGLQDSYPGLGGAQWPKSDRRVAWHKVYGATKKVMPTPFKRDPLEIDPEW
KDAHLPCTFQVSYSDHIYSRITVPIDAETSRVFYFHATMPLTRTKRIWDHVYFEVFQNWMMNYNFSGQDEKVVRHQAYDT
PETFSATDVFPLTLRKLILENARDFHLKDGHDTEWPDAAGATSAKEDSK

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=126, Percent_Identity=35.7142857142857, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161085896, Length=136, Percent_Identity=30.8823529411765, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 50271; Mature: 50140

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSAGARPAAAEDENTNSLHPSALRERIPEMGLKEYWYPAIEAKKVKSGKPTTVKMLGQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHCCCCCCCCEEEEHHC
DLSFFRGKDGNVVATSSTCPHRGASLAHGKCHFAGTITCPYHGWTFDETGACVAVLGEGP
CHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCC
ESRIPGMPDARVRSYPTQTHKGIVFAWMGESDPAPIEEDVPPQFFNDEFLIQVSVTSWNC
HHCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECC
NWRPAFENLLDSHVFYVHRNSVHLMMMDAANLLIASKMGPRRPRPRVVNGRALMYPPGAL
CCCHHHHHHHHCEEEEEECCCEEEEEECCCCEEEECCCCCCCCCCEEECCEEEEECCCCC
GFADAFVGTGAKHAAEKATATEVKTWPPAPEAGLQDSYPGLGGAQWPKSDRRVAWHKVYG
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHC
ATKKVMPTPFKRDPLEIDPEWKDAHLPCTFQVSYSDHIYSRITVPIDAETSRVFYFHATM
CCCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEEECC
PLTRTKRIWDHVYFEVFQNWMMNYNFSGQDEKVVRHQAYDTPETFSATDVFPLTLRKLIL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ENARDFHLKDGHDTEWPDAAGATSAKEDSK
HCCCCEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGSAGARPAAAEDENTNSLHPSALRERIPEMGLKEYWYPAIEAKKVKSGKPTTVKMLGQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHCCCCCCCCEEEEHHC
DLSFFRGKDGNVVATSSTCPHRGASLAHGKCHFAGTITCPYHGWTFDETGACVAVLGEGP
CHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCC
ESRIPGMPDARVRSYPTQTHKGIVFAWMGESDPAPIEEDVPPQFFNDEFLIQVSVTSWNC
HHCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECC
NWRPAFENLLDSHVFYVHRNSVHLMMMDAANLLIASKMGPRRPRPRVVNGRALMYPPGAL
CCCHHHHHHHHCEEEEEECCCEEEEEECCCCEEEECCCCCCCCCCEEECCEEEEECCCCC
GFADAFVGTGAKHAAEKATATEVKTWPPAPEAGLQDSYPGLGGAQWPKSDRRVAWHKVYG
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHC
ATKKVMPTPFKRDPLEIDPEWKDAHLPCTFQVSYSDHIYSRITVPIDAETSRVFYFHATM
CCCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEEECC
PLTRTKRIWDHVYFEVFQNWMMNYNFSGQDEKVVRHQAYDTPETFSATDVFPLTLRKLIL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ENARDFHLKDGHDTEWPDAAGATSAKEDSK
HCCCCEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704279 [H]