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The map label for this gene is sigF [H]

Identifier: 119716157

GI number: 119716157

Start: 2052035

End: 2052883

Strand: Reverse

Name: sigF [H]

Synonym: Noca_1925

Alternate gene names: 119716157

Gene position: 2052883-2052035 (Counterclockwise)

Preceding gene: 119716160

Following gene: 119716156

Centisome position: 41.17

GC content: 73.38

Gene sequence:

>849_bases
ATGACCACGTTGCCCGCCCCCTCCACCGACGCTCCTCCGGACCGGCTCGACCCGAGCCTGGACCCCACGCGGGACCCCGA
CTGCGGCCTGACCCGCCCCGAGCGCGCCGAGCACACGGCGCGCCTGCTCGACGAGGCCCAGCACAGCGACGACGCAGGCG
AGCGCGAGGAGGTGCTCGGCCGGGTCGTCGTGATCAACCGCGGTGTCGCGGATGCGGTCGCCGCCCGGTTCCGCAACCGC
GGGATCCCCCAGGAGGACCTGCGCCAGGTCGCCTACGAGGGCCTGACCAAGGCCGTGCACCGGTTCGACCCCGAGCTGCG
CAACGACCTGCTCACCTACGCCGTCCCCACCATCCGCGGCGAGCTGCAGCGCTACTTCCGCGACCAGGGCTGGACGGTGC
GGCCCCCGCGGCGCATCCAGGAGCTCCAGTGGCGGGTGAACCAGGTGATCGAGCGGCTCACCCAGGAGCGCGGCCGGGAG
CCCTCCGAGGACGAGATCGCCGCCGAGCTCGACACCACGGTCGAGGAGTACCGCGACGCCGCCTCGGCCTTCGGCTGCTT
CCAGCCCACCTCCCTCGACCAGCCAGTGGGCCACGAGGGCGAGACTCCGGTCGGCGAGCTGATCGCCGGTGCGGACGCCG
ACGGGCCCGCGACCGAGGCCCGGATGCTGCTCGCGCCCGTCGTACGCCGGCTCCCCGAGCGGCACCGCCGGATCCTCTAC
CTGCGGTTCTTCGAGGACCTCACCCAGGAGGAGATCGGCCGCGACCTCGGCGTCACCCAGATGCAGGTCTCGCGCCTGCT
CAGCCGGATCCTCGGGGACCTCAGGGACGAGCTCAAGGAGGCCGGGTGA

Upstream 100 bases:

>100_bases
GCTGCCGGGCTCAGCCGGTAGCGTCGTCGGGAGGAGACGGACGCACGAACCTTGCTGAAGAAGCCGGCAAGGACCCATCA
CTCGGGATGGAGAACGCCCG

Downstream 100 bases:

>100_bases
GCGGACCCCGCACGCTCGCGGCACAGTTCGCCGAGCTGAGCGGCGACCTCCTGAGCGACCCCGACGAGACGGTCACGTTC
GAGCGGGTGGTCCAGCGCGC

Product: sigma-70 region 4 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTTLPAPSTDAPPDRLDPSLDPTRDPDCGLTRPERAEHTARLLDEAQHSDDAGEREEVLGRVVVINRGVADAVAARFRNR
GIPQEDLRQVAYEGLTKAVHRFDPELRNDLLTYAVPTIRGELQRYFRDQGWTVRPPRRIQELQWRVNQVIERLTQERGRE
PSEDEIAAELDTTVEEYRDAASAFGCFQPTSLDQPVGHEGETPVGELIAGADADGPATEARMLLAPVVRRLPERHRRILY
LRFFEDLTQEEIGRDLGVTQMQVSRLLSRILGDLRDELKEAG

Sequences:

>Translated_282_residues
MTTLPAPSTDAPPDRLDPSLDPTRDPDCGLTRPERAEHTARLLDEAQHSDDAGEREEVLGRVVVINRGVADAVAARFRNR
GIPQEDLRQVAYEGLTKAVHRFDPELRNDLLTYAVPTIRGELQRYFRDQGWTVRPPRRIQELQWRVNQVIERLTQERGRE
PSEDEIAAELDTTVEEYRDAASAFGCFQPTSLDQPVGHEGETPVGELIAGADADGPATEARMLLAPVVRRLPERHRRILY
LRFFEDLTQEEIGRDLGVTQMQVSRLLSRILGDLRDELKEAG
>Mature_281_residues
TTLPAPSTDAPPDRLDPSLDPTRDPDCGLTRPERAEHTARLLDEAQHSDDAGEREEVLGRVVVINRGVADAVAARFRNRG
IPQEDLRQVAYEGLTKAVHRFDPELRNDLLTYAVPTIRGELQRYFRDQGWTVRPPRRIQELQWRVNQVIERLTQERGREP
SEDEIAAELDTTVEEYRDAASAFGCFQPTSLDQPVGHEGETPVGELIAGADADGPATEARMLLAPVVRRLPERHRRILYL
RFFEDLTQEEIGRDLGVTQMQVSRLLSRILGDLRDELKEAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=223, Percent_Identity=27.8026905829596, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=30.9012875536481, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788231, Length=210, Percent_Identity=30, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31888; Mature: 31757

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLPAPSTDAPPDRLDPSLDPTRDPDCGLTRPERAEHTARLLDEAQHSDDAGEREEVLG
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
RVVVINRGVADAVAARFRNRGIPQEDLRQVAYEGLTKAVHRFDPELRNDLLTYAVPTIRG
HHHHEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ELQRYFRDQGWTVRPPRRIQELQWRVNQVIERLTQERGREPSEDEIAAELDTTVEEYRDA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ASAFGCFQPTSLDQPVGHEGETPVGELIAGADADGPATEARMLLAPVVRRLPERHRRILY
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRFFEDLTQEEIGRDLGVTQMQVSRLLSRILGDLRDELKEAG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTLPAPSTDAPPDRLDPSLDPTRDPDCGLTRPERAEHTARLLDEAQHSDDAGEREEVLG
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
RVVVINRGVADAVAARFRNRGIPQEDLRQVAYEGLTKAVHRFDPELRNDLLTYAVPTIRG
HHHHEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ELQRYFRDQGWTVRPPRRIQELQWRVNQVIERLTQERGREPSEDEIAAELDTTVEEYRDA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ASAFGCFQPTSLDQPVGHEGETPVGELIAGADADGPATEARMLLAPVVRRLPERHRRILY
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRFFEDLTQEEIGRDLGVTQMQVSRLLSRILGDLRDELKEAG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207 [H]