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The map label for this gene is kstD [H]

Identifier: 119716084

GI number: 119716084

Start: 1974566

End: 1976164

Strand: Direct

Name: kstD [H]

Synonym: Noca_1851

Alternate gene names: 119716084

Gene position: 1974566-1976164 (Clockwise)

Preceding gene: 119716083

Following gene: 119716085

Centisome position: 39.6

GC content: 72.67

Gene sequence:

>1599_bases
GTGACCGCGAGGACCGAGTTCGACGTCGTCGTCGTCGGCTCCGGCGCGGCCGGGCTCGTCGCCGCGCTGACGGCGGCGCA
CGCTGGCGCGTCGGTGCTGGTGCTCGAGGCGACCGACCGCTGGGGCGGCTCGACGGCCGTGTCCGGGGGACTGGTCTGGG
CGCCGGACCACCACCACGCCGACGAGAAGGGTATGAGCGACTCGCGGGAGGAGGTGCTGACGCACCTGCGCGGCTGCGCC
TACGCCCGCGACGACGCCCGTCTCGTCGCCTTCGTCGACGCCAGCCCGCAGGCGGTGCGCTTCGTCGAGGAGCACACCCC
GCTCACCTTCCGGGCGATCGACTACCCCGACACGTTCTCGGAGCGTGCGGGCGGCAAGCTGCACCGGCACCTCGAGTCGC
TGCCCCAGGACTCGAGTCCGCTCGGCCGCTGGCAGGACCTCTTCTGGACCCAGGACGAGCCGCATACGCTGATGAGCGAG
ATCCTGCCGATCGGCGCCCACGTCTACGGCCTGCCGCTCGACCTGCCCAGCGTCACCGAGCGGCGTGAGCGCGGCCTCGT
CACCGGCGGTACGGCGCTGGTCACCGGCCTCCTGCACGGGGTCGACGCCGCAGGTGTCATCCGGCGGAAGAACGCCCGTG
TTGATGCCTTGGTGGTCGAGAACGGTCGGGTCCGGGGAGTCCGCACCGGGGTCGAGGTGATCGGCGCCCGCCGCGGCGTC
GTGCTCGCCTCGGGTGGGTTCGAGTGGAACGACGACCTCAAGGGGCGCCACCTGGCGGTGCCGATCACCCACCCGGTCAC
CCCGCCGGTGCAGCACGGTGACGCCTTCCGGCTCGCCGGGACGGTCGGTGCGGCGTTCGCACACACCAGCGAGAACTGGG
CGTGGCCCGCGGTCGAGGTGCCCACCGACACCTGGCCGGACGGGACGCCCCGGCACCACCTCTCCTTCAGCGAGCGTTAC
ATGCCGCATTGCCTGTGGGTGAACGGCGCTGGCCGGCGCTTCGTCAACGAGTCCAGCCACAACGCAGCGCTGTCGCTGGG
AGAGGTGGACACCACCTCGCACACCCCGCGCAATCTGCCCGCGTGGTCGGTGATGGATGCACAGTTCCGCGCCCGCTACC
AGGTGGCCGGAGTGCTGCCGGGCGAGGCGGCCACGGGCTGGATCGTCGAGGCCCCTACGGTGGCCGACCTCGCGGAGCGG
ATCGGCGTCGACCCGACCGCGCTGGCCGAGACCATCGACCGGTTCAACACCCACGCGAAGGCGGGCTCCGACCCCGACTT
CGGCCGGGGACGGAGCGCCTACGAACGGGCCATGGGCGACCCGACCGCGGAGTTCCCGAACATCGGCACGGTCGAGGTCG
GCCCGTTCACCGCGGTCGCGATCCGGCCGAGCACCGTCGGTACCAAGGGCGGGGTCCTCACCAACGCGAACGCCGAGGCC
CTCGACCACGACGGTGACCCGGTCCCGGGTCTGTACGCCGCCGGCAACGCGATGGCCGCGGTCTTCGGACCGGGCATCTC
CGGCGGCGGCATGACGATCGGCAACGCGGTCGCCTGGGGCTGGCTGGCCGGGACGAACGCGGCTCGGTCGCGCTCATGA

Upstream 100 bases:

>100_bases
TCACCCTGGAGGAGGGCGTCACCGTCGTGTACGAGGGCACCGCCGGAACGCGCGCCTTCTTCTCGCTCGTCCCCGCCGAC
TGGATGGAGCGGACCGCGCA

Downstream 100 bases:

>100_bases
GCGCGCCGATCCTGGTGCTCGGGGCCACCGGCAAGACCGGAGCGCCGGTGGTCGCCGAGCTCGCCCGCCGCGGGGCTGCC
CACCGAGCCGCGAAGCGGCG

Product: fumarate reductase/succinate dehydrogenase flavoprotein domain-containing protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTARTEFDVVVVGSGAAGLVAALTAAHAGASVLVLEATDRWGGSTAVSGGLVWAPDHHHADEKGMSDSREEVLTHLRGCA
YARDDARLVAFVDASPQAVRFVEEHTPLTFRAIDYPDTFSERAGGKLHRHLESLPQDSSPLGRWQDLFWTQDEPHTLMSE
ILPIGAHVYGLPLDLPSVTERRERGLVTGGTALVTGLLHGVDAAGVIRRKNARVDALVVENGRVRGVRTGVEVIGARRGV
VLASGGFEWNDDLKGRHLAVPITHPVTPPVQHGDAFRLAGTVGAAFAHTSENWAWPAVEVPTDTWPDGTPRHHLSFSERY
MPHCLWVNGAGRRFVNESSHNAALSLGEVDTTSHTPRNLPAWSVMDAQFRARYQVAGVLPGEAATGWIVEAPTVADLAER
IGVDPTALAETIDRFNTHAKAGSDPDFGRGRSAYERAMGDPTAEFPNIGTVEVGPFTAVAIRPSTVGTKGGVLTNANAEA
LDHDGDPVPGLYAAGNAMAAVFGPGISGGGMTIGNAVAWGWLAGTNAARSRS

Sequences:

>Translated_532_residues
MTARTEFDVVVVGSGAAGLVAALTAAHAGASVLVLEATDRWGGSTAVSGGLVWAPDHHHADEKGMSDSREEVLTHLRGCA
YARDDARLVAFVDASPQAVRFVEEHTPLTFRAIDYPDTFSERAGGKLHRHLESLPQDSSPLGRWQDLFWTQDEPHTLMSE
ILPIGAHVYGLPLDLPSVTERRERGLVTGGTALVTGLLHGVDAAGVIRRKNARVDALVVENGRVRGVRTGVEVIGARRGV
VLASGGFEWNDDLKGRHLAVPITHPVTPPVQHGDAFRLAGTVGAAFAHTSENWAWPAVEVPTDTWPDGTPRHHLSFSERY
MPHCLWVNGAGRRFVNESSHNAALSLGEVDTTSHTPRNLPAWSVMDAQFRARYQVAGVLPGEAATGWIVEAPTVADLAER
IGVDPTALAETIDRFNTHAKAGSDPDFGRGRSAYERAMGDPTAEFPNIGTVEVGPFTAVAIRPSTVGTKGGVLTNANAEA
LDHDGDPVPGLYAAGNAMAAVFGPGISGGGMTIGNAVAWGWLAGTNAARSRS
>Mature_531_residues
TARTEFDVVVVGSGAAGLVAALTAAHAGASVLVLEATDRWGGSTAVSGGLVWAPDHHHADEKGMSDSREEVLTHLRGCAY
ARDDARLVAFVDASPQAVRFVEEHTPLTFRAIDYPDTFSERAGGKLHRHLESLPQDSSPLGRWQDLFWTQDEPHTLMSEI
LPIGAHVYGLPLDLPSVTERRERGLVTGGTALVTGLLHGVDAAGVIRRKNARVDALVVENGRVRGVRTGVEVIGARRGVV
LASGGFEWNDDLKGRHLAVPITHPVTPPVQHGDAFRLAGTVGAAFAHTSENWAWPAVEVPTDTWPDGTPRHHLSFSERYM
PHCLWVNGAGRRFVNESSHNAALSLGEVDTTSHTPRNLPAWSVMDAQFRARYQVAGVLPGEAATGWIVEAPTVADLAERI
GVDPTALAETIDRFNTHAKAGSDPDFGRGRSAYERAMGDPTAEFPNIGTVEVGPFTAVAIRPSTVGTKGGVLTNANAEAL
DHDGDPVPGLYAAGNAMAAVFGPGISGGGMTIGNAVAWGWLAGTNAARSRS

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 56363; Mature: 56232

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARTEFDVVVVGSGAAGLVAALTAAHAGASVLVLEATDRWGGSTAVSGGLVWAPDHHHA
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCEEECCCCCCC
DEKGMSDSREEVLTHLRGCAYARDDARLVAFVDASPQAVRFVEEHTPLTFRAIDYPDTFS
CCCCCCCCHHHHHHHHHHCCEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHH
ERAGGKLHRHLESLPQDSSPLGRWQDLFWTQDEPHTLMSEILPIGAHVYGLPLDLPSVTE
HHCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHH
RRERGLVTGGTALVTGLLHGVDAAGVIRRKNARVDALVVENGRVRGVRTGVEVIGARRGV
HHHCCCEECCHHHHHHHHHCCCHHHHEEECCCCEEEEEECCCCEEEEHHHHHHHCCCCCE
VLASGGFEWNDDLKGRHLAVPITHPVTPPVQHGDAFRLAGTVGAAFAHTSENWAWPAVEV
EEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHEECCCCCCCCEEEC
PTDTWPDGTPRHHLSFSERYMPHCLWVNGAGRRFVNESSHNAALSLGEVDTTSHTPRNLP
CCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
AWSVMDAQFRARYQVAGVLPGEAATGWIVEAPTVADLAERIGVDPTALAETIDRFNTHAK
CCEEECHHHHCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
AGSDPDFGRGRSAYERAMGDPTAEFPNIGTVEVGPFTAVAIRPSTVGTKGGVLTNANAEA
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCEEECCCCHH
LDHDGDPVPGLYAAGNAMAAVFGPGISGGGMTIGNAVAWGWLAGTNAARSRS
CCCCCCCCCCEEECCCEEEEEECCCCCCCCEEECCHHEEHEEECCCCCCCCC
>Mature Secondary Structure 
TARTEFDVVVVGSGAAGLVAALTAAHAGASVLVLEATDRWGGSTAVSGGLVWAPDHHHA
CCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCEEECCCCCCC
DEKGMSDSREEVLTHLRGCAYARDDARLVAFVDASPQAVRFVEEHTPLTFRAIDYPDTFS
CCCCCCCCHHHHHHHHHHCCEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHH
ERAGGKLHRHLESLPQDSSPLGRWQDLFWTQDEPHTLMSEILPIGAHVYGLPLDLPSVTE
HHCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHH
RRERGLVTGGTALVTGLLHGVDAAGVIRRKNARVDALVVENGRVRGVRTGVEVIGARRGV
HHHCCCEECCHHHHHHHHHCCCHHHHEEECCCCEEEEEECCCCEEEEHHHHHHHCCCCCE
VLASGGFEWNDDLKGRHLAVPITHPVTPPVQHGDAFRLAGTVGAAFAHTSENWAWPAVEV
EEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHEECCCCCCCCEEEC
PTDTWPDGTPRHHLSFSERYMPHCLWVNGAGRRFVNESSHNAALSLGEVDTTSHTPRNLP
CCCCCCCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
AWSVMDAQFRARYQVAGVLPGEAATGWIVEAPTVADLAERIGVDPTALAETIDRFNTHAK
CCEEECHHHHCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
AGSDPDFGRGRSAYERAMGDPTAEFPNIGTVEVGPFTAVAIRPSTVGTKGGVLTNANAEA
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEECCCCCCCCCEEECCCCHH
LDHDGDPVPGLYAAGNAMAAVFGPGISGGGMTIGNAVAWGWLAGTNAARSRS
CCCCCCCCCCEEECCCEEEEEECCCCCCCCEEECCHHEEHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]