Click here to switch to the map view.

The map label for this gene is braC [H]

Identifier: 119716075

GI number: 119716075

Start: 1965841

End: 1967025

Strand: Direct

Name: braC [H]

Synonym: Noca_1842

Alternate gene names: 119716075

Gene position: 1965841-1967025 (Clockwise)

Preceding gene: 119716074

Following gene: 119716076

Centisome position: 39.43

GC content: 65.49

Gene sequence:

>1185_bases
ATGAAGTTCAAGAAGGCGTACCTCGCGGCTGGCGCCACGCTGGCGTTGGCGCTCTTCGTCAGCGCGTGCGGCAGCGACTC
GGGCGGGTCCGGCGGCGGTGGCGATGACACGATCACCGTGGCGGTCGCGGGTCCCATGACCGGAGACAACGGCATCTACG
GCCAGGATCAGCTGTCGGGCGTGCAGTTCGCAGCGAAGGAGATCAACGACTCGGGCGGGATCCCTGACGGTCCGTTGAAG
GGCAAGAAGATCAAGGTCGTCAAGTTCGACGACGTGGCCGACCCCAACCAGGGTGCGTCCGTGGCGCAGAAGATCTGTGA
CGACACCAGCATCATGGCGGTCTTCGGCCACAGCAACTCCTCGGTCACGCTCGCCGCGGAGCCGATCTACGAGCGCTGCG
GGGTGCCGCTCTTCGTCAGCTACTCGTCGAACCCGGAGATCACCGCGGAGCTACACGAGAACCTGTTCCGCACGCTCATC
GACGACGCCAAGATGGGCAGCGAGATGGCGAGTTTTTCCCACGACCAGCTCGGCTTCAAGAAGGTCGGCGTCATCGCCTC
CGACGACGACTACGGCGACGGCCTGAAGACCAACTTCAACAAGACGGCCGAGGAGATCGGCCTCGACGTCGCGAAGACCG
TCACGACGTCGGCGAAGCAGAAGGACTTCACGCCGCAGCTGACCGAGCTCCGCAACGCCGGTGCCGACTCGCTGGTGCTC
CTGAACACCTACACGGACGCCGCGCTGCAGATCAAGCAGGCCGACGCGATGGGCTGGGACGTCCCGATCTTCGTCACCCC
GGGCTCGAACAGCCCGGAGCTGGTCAAGATCGCCGGTGAGAAGGCGGCGGAGGGCACAATCGTCGCCGCGGTCTTCGACC
CCAACTCGAGCGAGCCGGGCCCGGCGAAGTTCGTCAACGACTTCACCGCCGCCAACGGCAAGGGTCCGGGCGAGTCCGCC
GCGATGTCCTACGACTCCTTCTACGTGTTCCTGACCTCCCTGGAGAAGGGTGCGAAGGACCGCAAGAGCGTCATCGAGAA
GTCCGCCGAGATCGGGACGTTCACACTCCCGATCCGCGGCGAGCTGATGTTCAACGAGACCCACGAGCCGACGGTCGTGC
CGGGCAAGCCCGCGCAGATCCTGCTCCAGGTCAAGGACGGCCAGATCGGCAGCTACGCCGGCTGA

Upstream 100 bases:

>100_bases
CGAAGCTTCCGGGCGGCGGGGGTGCCGCAGGTACGCGGTGACGGGACACCGGGTGCACGCGCGCCGCATTGCAGCACCAC
ATCCAGCACTAGGAGCATGT

Downstream 100 bases:

>100_bases
CCGCTCTCCGCAAGACCAGGGTGCCGTGCAGGGGAGTCCCCTGCCCTGCACGGCACCCGTCCCACCCCTTCGCCCCTGAC
CTCGAACCAGCAAGGAGCCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKFKKAYLAAGATLALALFVSACGSDSGGSGGGGDDTITVAVAGPMTGDNGIYGQDQLSGVQFAAKEINDSGGIPDGPLK
GKKIKVVKFDDVADPNQGASVAQKICDDTSIMAVFGHSNSSVTLAAEPIYERCGVPLFVSYSSNPEITAELHENLFRTLI
DDAKMGSEMASFSHDQLGFKKVGVIASDDDYGDGLKTNFNKTAEEIGLDVAKTVTTSAKQKDFTPQLTELRNAGADSLVL
LNTYTDAALQIKQADAMGWDVPIFVTPGSNSPELVKIAGEKAAEGTIVAAVFDPNSSEPGPAKFVNDFTAANGKGPGESA
AMSYDSFYVFLTSLEKGAKDRKSVIEKSAEIGTFTLPIRGELMFNETHEPTVVPGKPAQILLQVKDGQIGSYAG

Sequences:

>Translated_394_residues
MKFKKAYLAAGATLALALFVSACGSDSGGSGGGGDDTITVAVAGPMTGDNGIYGQDQLSGVQFAAKEINDSGGIPDGPLK
GKKIKVVKFDDVADPNQGASVAQKICDDTSIMAVFGHSNSSVTLAAEPIYERCGVPLFVSYSSNPEITAELHENLFRTLI
DDAKMGSEMASFSHDQLGFKKVGVIASDDDYGDGLKTNFNKTAEEIGLDVAKTVTTSAKQKDFTPQLTELRNAGADSLVL
LNTYTDAALQIKQADAMGWDVPIFVTPGSNSPELVKIAGEKAAEGTIVAAVFDPNSSEPGPAKFVNDFTAANGKGPGESA
AMSYDSFYVFLTSLEKGAKDRKSVIEKSAEIGTFTLPIRGELMFNETHEPTVVPGKPAQILLQVKDGQIGSYAG
>Mature_394_residues
MKFKKAYLAAGATLALALFVSACGSDSGGSGGGGDDTITVAVAGPMTGDNGIYGQDQLSGVQFAAKEINDSGGIPDGPLK
GKKIKVVKFDDVADPNQGASVAQKICDDTSIMAVFGHSNSSVTLAAEPIYERCGVPLFVSYSSNPEITAELHENLFRTLI
DDAKMGSEMASFSHDQLGFKKVGVIASDDDYGDGLKTNFNKTAEEIGLDVAKTVTTSAKQKDFTPQLTELRNAGADSLVL
LNTYTDAALQIKQADAMGWDVPIFVTPGSNSPELVKIAGEKAAEGTIVAAVFDPNSSEPGPAKFVNDFTAANGKGPGESA
AMSYDSFYVFLTSLEKGAKDRKSVIEKSAEIGTFTLPIRGELMFNETHEPTVVPGKPAQILLQVKDGQIGSYAG

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=307, Percent_Identity=29.6416938110749, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI48994941, Length=301, Percent_Identity=29.9003322259136, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41220; Mature: 41220

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKKAYLAAGATLALALFVSACGSDSGGSGGGGDDTITVAVAGPMTGDNGIYGQDQLSG
CCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCC
VQFAAKEINDSGGIPDGPLKGKKIKVVKFDDVADPNQGASVAQKICDDTSIMAVFGHSNS
HHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEECCCC
SVTLAAEPIYERCGVPLFVSYSSNPEITAELHENLFRTLIDDAKMGSEMASFSHDQLGFK
EEEEEECCHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHCHHHHHCCCHHCCCE
KVGVIASDDDYGDGLKTNFNKTAEEIGLDVAKTVTTSAKQKDFTPQLTELRNAGADSLVL
EEEEEECCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEE
LNTYTDAALQIKQADAMGWDVPIFVTPGSNSPELVKIAGEKAAEGTIVAAVFDPNSSEPG
EEECCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCC
PAKFVNDFTAANGKGPGESAAMSYDSFYVFLTSLEKGAKDRKSVIEKSAEIGTFTLPIRG
HHHHHHHHHCCCCCCCCCHHEEECCCEEEEEEHHHHCCHHHHHHHHHHCCCCEEEEEEEE
ELMFNETHEPTVVPGKPAQILLQVKDGQIGSYAG
EEEECCCCCCEEECCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKFKKAYLAAGATLALALFVSACGSDSGGSGGGGDDTITVAVAGPMTGDNGIYGQDQLSG
CCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCC
VQFAAKEINDSGGIPDGPLKGKKIKVVKFDDVADPNQGASVAQKICDDTSIMAVFGHSNS
HHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEECCCC
SVTLAAEPIYERCGVPLFVSYSSNPEITAELHENLFRTLIDDAKMGSEMASFSHDQLGFK
EEEEEECCHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHCHHHHHCCCHHCCCE
KVGVIASDDDYGDGLKTNFNKTAEEIGLDVAKTVTTSAKQKDFTPQLTELRNAGADSLVL
EEEEEECCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEE
LNTYTDAALQIKQADAMGWDVPIFVTPGSNSPELVKIAGEKAAEGTIVAAVFDPNSSEPG
EEECCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCC
PAKFVNDFTAANGKGPGESAAMSYDSFYVFLTSLEKGAKDRKSVIEKSAEIGTFTLPIRG
HHHHHHHHHCCCCCCCCCHHEEECCCEEEEEEHHHHCCHHHHHHHHHHCCCCEEEEEEEE
ELMFNETHEPTVVPGKPAQILLQVKDGQIGSYAG
EEEECCCCCCEEECCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]