BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is echA8 [H]

Identifier: 119716047

GI number: 119716047

Start: 1934626

End: 1935492

Strand: Direct

Name: echA8 [H]

Synonym: Noca_1813

Alternate gene names: 119716047

Gene position: 1934626-1935492 (Clockwise)

Preceding gene: 119716045

Following gene: 119716048

Centisome position: 38.8

GC content: 70.82

Gene sequence:

>867_bases
TTGTTCCCGATGACGGCACAATGCCGGGGAGCGACCATCGAGATCGGCGACCGATTCGGCAACCAGGACAGAGAGGCGGG
TCACGGCATGGGTGAGTTCGTACGACTGGAGGTGGCCGACGGCGTCGGCACGATCCGGCTGGACCGGCCGAAGATGAACG
CCCTGAACGTGCAGGTGCAGGAGGAGATCCGCGCGGCGGCCGTCGAGGCCACCGAGCGCGACGACGTGAAGGCCGTGGTG
GTGTACGGCGGCGAGCGGGTCTTCGCCGCGGGCGCCGATATCAAGGAGATGGCCGACATGTCGTACACCGACATGGTCAA
GCGCTCCGGCCCACTCCAGTCCGCGCTCGGCGCGGTGGCCCGGATCCCGAAGCCCGTGGTCGCCGCGATCACCGGCTACG
CGCTCGGCGGCGGCTGCGAGCTGGCGCTGTGCGCGGATGTCCGGTTCGCCGCCGAGGACGCGGTCCTCGGCCAGCCCGAG
GTGCTGCTCGGCATCATCCCGGGGGCCGGCGGCACCCAGCGGCTGACCCGACTGGTCGGCCCGAGCAAGGCGAAGGACAT
CGTCTTCACCGGCCGGTTCGTCAAGGCCGACGAGGCGCTGGCGATCGGCCTGGTCGACCGGGTCTTCCCGGCCGCGTCGG
TCTACGACGAGGCGCTCGCGTGGGCGGGCCGGTTCGCCGGGGCGGCGTCGTACGCACTGCGGGCCGCGAAGGAGAGCATC
GACCGCGGCATCGAGGTCGACCTGGAGACGGGCCTGGAGATCGAGCGGCAGCAGTTCGCGGCGCTGTTCGCGACCGAGGA
CCGCTCGATCGGTATGCGATCCTTCGTCGAGAACGGACCGGGCAAGGCGCAGTTCGAGGGCAGGTGA

Upstream 100 bases:

>100_bases
GCCTCCGCCGACCAGGTCGATCCCAGCGGCCAGCTGCGTCCCGGCCACACCGGTGCGCGCTCGCCGCGGTCGCGCCAGAG
TCCGGGGGTCACCCACCCCA

Downstream 100 bases:

>100_bases
GCGGTACGTGGCGGGTCGCAAGACGCTGACGGCGCGGCGGGGCTCCCGGGCGCGCGCCCGCGCGGCGCAGGTGGTGTGGC
TGCTCGCCGTGCTGTGCGCG

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MFPMTAQCRGATIEIGDRFGNQDREAGHGMGEFVRLEVADGVGTIRLDRPKMNALNVQVQEEIRAAAVEATERDDVKAVV
VYGGERVFAAGADIKEMADMSYTDMVKRSGPLQSALGAVARIPKPVVAAITGYALGGGCELALCADVRFAAEDAVLGQPE
VLLGIIPGAGGTQRLTRLVGPSKAKDIVFTGRFVKADEALAIGLVDRVFPAASVYDEALAWAGRFAGAASYALRAAKESI
DRGIEVDLETGLEIERQQFAALFATEDRSIGMRSFVENGPGKAQFEGR

Sequences:

>Translated_288_residues
MFPMTAQCRGATIEIGDRFGNQDREAGHGMGEFVRLEVADGVGTIRLDRPKMNALNVQVQEEIRAAAVEATERDDVKAVV
VYGGERVFAAGADIKEMADMSYTDMVKRSGPLQSALGAVARIPKPVVAAITGYALGGGCELALCADVRFAAEDAVLGQPE
VLLGIIPGAGGTQRLTRLVGPSKAKDIVFTGRFVKADEALAIGLVDRVFPAASVYDEALAWAGRFAGAASYALRAAKESI
DRGIEVDLETGLEIERQQFAALFATEDRSIGMRSFVENGPGKAQFEGR
>Mature_288_residues
MFPMTAQCRGATIEIGDRFGNQDREAGHGMGEFVRLEVADGVGTIRLDRPKMNALNVQVQEEIRAAAVEATERDDVKAVV
VYGGERVFAAGADIKEMADMSYTDMVKRSGPLQSALGAVARIPKPVVAAITGYALGGGCELALCADVRFAAEDAVLGQPE
VLLGIIPGAGGTQRLTRLVGPSKAKDIVFTGRFVKADEALAIGLVDRVFPAASVYDEALAWAGRFAGAASYALRAAKESI
DRGIEVDLETGLEIERQQFAALFATEDRSIGMRSFVENGPGKAQFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=42.741935483871, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI4502327, Length=226, Percent_Identity=40.2654867256637, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI68989263, Length=236, Percent_Identity=36.0169491525424, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI20127408, Length=173, Percent_Identity=39.3063583815029, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI70995211, Length=251, Percent_Identity=30.6772908366534, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=27.0742358078603, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=27.0742358078603, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=33.739837398374, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=284, Percent_Identity=25.7042253521127, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI45643119, Length=208, Percent_Identity=28.8461538461538, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI260275230, Length=208, Percent_Identity=28.8461538461538, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI260274832, Length=208, Percent_Identity=28.8461538461538, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=27.5, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI261878539, Length=132, Percent_Identity=39.3939393939394, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI25453481, Length=230, Percent_Identity=25.2173913043478, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI51538573, Length=230, Percent_Identity=25.2173913043478, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI221136756, Length=254, Percent_Identity=24.4094488188976, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136753, Length=254, Percent_Identity=24.4094488188976, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757968, Length=230, Percent_Identity=24.3478260869565, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=230, Percent_Identity=24.3478260869565, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=244, Percent_Identity=25.4098360655738, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757966, Length=217, Percent_Identity=24.8847926267281, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538581, Length=217, Percent_Identity=24.8847926267281, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI116235487, Length=230, Percent_Identity=25.2173913043478, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI62530384, Length=274, Percent_Identity=24.8175182481752, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=42.2924901185771, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=34.1176470588235, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=37.8238341968912, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790281, Length=185, Percent_Identity=36.2162162162162, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=30.5555555555556, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=26.5917602996255, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787660, Length=243, Percent_Identity=29.2181069958848, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=42.7480916030534, Blast_Score=191, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=39.3574297188755, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=33.7777777777778, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17558304, Length=184, Percent_Identity=37.5, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=39.8907103825137, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=39.8907103825137, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=39.8907103825137, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=249, Percent_Identity=27.3092369477912, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=260, Percent_Identity=29.6153846153846, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=28.8537549407115, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=238, Percent_Identity=26.4705882352941, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=149, Percent_Identity=30.8724832214765, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=149, Percent_Identity=30.8724832214765, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI20129971, Length=273, Percent_Identity=40.2930402930403, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24653477, Length=273, Percent_Identity=40.2930402930403, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=36.5671641791045, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19920382, Length=241, Percent_Identity=32.3651452282158, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357171, Length=254, Percent_Identity=31.1023622047244, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=37.9120879120879, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=37.9120879120879, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=37.9120879120879, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=33.8461538461538, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921018, Length=199, Percent_Identity=27.6381909547739, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=21.5094339622642, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583165, Length=234, Percent_Identity=24.7863247863248, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24654903, Length=223, Percent_Identity=24.2152466367713, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 30569; Mature: 30569

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPMTAQCRGATIEIGDRFGNQDREAGHGMGEFVRLEVADGVGTIRLDRPKMNALNVQVQ
CCCCCCCCCCCEEEECCCCCCCCCHHCCCCCCEEEEEEECCCEEEEECCCCCCEEEEEHH
EEIRAAAVEATERDDVKAVVVYGGERVFAAGADIKEMADMSYTDMVKRSGPLQSALGAVA
HHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCHHHHHCCCHHHHHHHCCCHHHHHHHHH
RIPKPVVAAITGYALGGGCELALCADVRFAAEDAVLGQPEVLLGIIPGAGGTQRLTRLVG
HCCCHHHHHHHHEEECCCCEEEEEECCEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHC
PSKAKDIVFTGRFVKADEALAIGLVDRVFPAASVYDEALAWAGRFAGAASYALRAAKESI
CCCCCEEEEECCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRGIEVDLETGLEIERQQFAALFATEDRSIGMRSFVENGPGKAQFEGR
HCCCEEEECCCCCHHHHHHHHHEECCCCCCCHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MFPMTAQCRGATIEIGDRFGNQDREAGHGMGEFVRLEVADGVGTIRLDRPKMNALNVQVQ
CCCCCCCCCCCEEEECCCCCCCCCHHCCCCCCEEEEEEECCCEEEEECCCCCCEEEEEHH
EEIRAAAVEATERDDVKAVVVYGGERVFAAGADIKEMADMSYTDMVKRSGPLQSALGAVA
HHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCHHHHHCCCHHHHHHHCCCHHHHHHHHH
RIPKPVVAAITGYALGGGCELALCADVRFAAEDAVLGQPEVLLGIIPGAGGTQRLTRLVG
HCCCHHHHHHHHEEECCCCEEEEEECCEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHC
PSKAKDIVFTGRFVKADEALAIGLVDRVFPAASVYDEALAWAGRFAGAASYALRAAKESI
CCCCCEEEEECCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRGIEVDLETGLEIERQQFAALFATEDRSIGMRSFVENGPGKAQFEGR
HCCCEEEECCCCCHHHHHHHHHEECCCCCCCHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]