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The map label for this gene is glgX [H]

Identifier: 119716046

GI number: 119716046

Start: 1932461

End: 1934617

Strand: Reverse

Name: glgX [H]

Synonym: Noca_1812

Alternate gene names: 119716046

Gene position: 1934617-1932461 (Counterclockwise)

Preceding gene: 119716051

Following gene: 119716044

Centisome position: 38.8

GC content: 70.84

Gene sequence:

>2157_bases
GTGACCCCCGGACTCTGGCGCGACCGCGGCGAGCGCGCACCGGTGTGGCCGGGACGCAGCTGGCCGCTGGGATCGACCTG
GTCGGCGGAGGCCACGAACTTCGCGGTGCACGCCCCCCGAGCCACCGCCGCCTGGGTCTGCCTTTTCGACGAGGAGGGCC
GCGAGCGCCGGCACCCACTCACCGAGCAGGAGCTCGGCATCTGGCACGGCGCGATCCCCGGCGTCGCCCCGGGAACCCGC
TACGGCTACCGGGTCGACGGGCCCTGGGACCCCGCGCAGGGGCTGCGGTTCAACGCGGACAAGCTGCTGCTCGACCCGTT
CGCCCGGGCGGTCTCCGGCGACCTGACCCTCGACCCGGCGATCTTCGGGTACGTCGAGGGCGCGCCCGACCGGCGCAGCG
ACACCGACTCGGCGCCGTACGTGCCGAGGAGCGTCGTCCTCGGCGACGACGACTTCGACTGGGACGACGACGCGCCGCCG
CGGCACCCGTGGCAGGACTCCGCGATCTACGAGATGCACGTCAAGGGCATGACCGCCCTGCACGACCGGGTGCCCGAGGA
GCTGCGCGGCACCTACGCCGGCCTGGCGACGCCCGCGGTCGTCGACTACCTCAAGGACCTCGGCATCACCGCCGTCGAGC
TGCTGCCGATCCACCAGTTCGTCTCGGAGACCTCGCTCCTGGAGCGCGGGCTGGTCAACTACTGGGGCTACAACACCATC
GGGTTCTTCGCCCCGCACAACGCCTACTCGTCCTCGGGCGACCGCGGCCAGCAGGTCACCGAGTTCAAGGCCATGGTCAA
GGCGTTCCACGCGGCCGGGCTCGAGGTGATCCTCGACGTCGTCTACAACCACACCGCCGAGGCCGGGACGACGGGTCCGA
CGCTGTCCTTTCGCGGCCTCGACGACCGCGGGTTCTACTACCGCACCGGCGGCGACGGGCACGCCGAGCCGTTCGAGGAC
ACCTACTGGGACGTCACCGGCTGTGGCAACACCGTCGACGCGTCCAACCCGTTCGCGCTGCGGCTGATCCTCGACTCGCT
GCGCTACTGGGTGACCGAGATGCACGTGGACGGCTTCCGCTTCGACCTGATGTCGGCGTTGACCCGGGTGCACCGGCAGA
TCGACATGCGCAGCCACCTGCTCACCGCGATCGGCCAGGACCCGGTCCTGCGGCACGTGAAGCTGATCGCCGAGCCGTGG
GACGCCTCGATGGACGGCTACCGGGTCGGTGAGTTCCCGCCGCCGTGGGTGGAGTGGAACGACCAGTACCGCGACGAGAT
CCGGGACTTCTGGCGCAACCACACCTCGGGCATCCGCGGGGTCGCCACCCGGCTGGCCGGGTCTTCGGACCTGTACCTCG
ACGACGGGCGCTCGCCGTACGCCTCGGTCAACTTCGTCACCGCCCACGACGGCTTCACGATGCGCGACCTCGTGACCTAC
GAGCGCAAGCGCAACGAGGCCAACGGCGAGGACAACCGCGACGGCACGGACAACAACCGCTCGTGGAACCACGGCGTCGA
GGGCGAGAGCCACGACCCGGCGCTCGTGGCCATCCGTCGTCGCCAGGTCGCGAACATGATGGCGACCCTGTGCCTGTCCA
ACGGGGTGCCGATGATCACCGCCGGCGATGAGCGCGGCCGCACCCAGGGCGGCAACAACAACGCCTACGCCCAGGACAAC
GAGACGTCCTGGATCGACTGGCGTCCCGACGACGCGTGGCTCGACGTCTACGAAGTCACCAAGACGGCGCTCCGGCTACG
CCGCGAGCACCCGGCGCTGCGGCAGCGGCACTGGTTCGAGGGCCGACCGACGCTGATCGGCGGCCCCAAGGACCTGGCCT
GGCTGCACCCCTCCGGTCGGGAGATGAGCGGCGACGACTGGCACGACCCGACCCTGCGCGCGGTCGGCATGTTCGTCTCC
GGCGCGCCGCTGCGCGCACCGGGGCCACGCGGCGAGCAGCAGGCCGACAAGTCGTTCGTGCTGTGGTTCAACTCCGACTG
GCTGCCGCAGCGGGTCACGCTGCCGGAGAACGACTGGGTACGTGCCGGCGAGGTGGTGCTCTCGACCGACTTCAGGCTCC
CTGTCGGCACCCCGGTCAAGGCCGGCGACCAGGTCCACATCGGGCGGCGCACGGTCGTCGTCTTCCGCGAGACCTGA

Upstream 100 bases:

>100_bases
CCCATGCCGTGACCCGCCTCTCTGTCCTGGTTGCCGAATCGGTCGCCGATCTCGATGGTCGCTCCCCGGCATTGTGCCGT
CATCGGGAACAATGGGGTGG

Downstream 100 bases:

>100_bases
CCGCGTCTGGCGTGTCGGGGCTTCGCCCCGCCACGCCGGGTCGCGGACTCGGTCCTGGGCGGCCCATCCGACGGTGTGGT
TGCGGTCGGCGCGTGGCCGC

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MTPGLWRDRGERAPVWPGRSWPLGSTWSAEATNFAVHAPRATAAWVCLFDEEGRERRHPLTEQELGIWHGAIPGVAPGTR
YGYRVDGPWDPAQGLRFNADKLLLDPFARAVSGDLTLDPAIFGYVEGAPDRRSDTDSAPYVPRSVVLGDDDFDWDDDAPP
RHPWQDSAIYEMHVKGMTALHDRVPEELRGTYAGLATPAVVDYLKDLGITAVELLPIHQFVSETSLLERGLVNYWGYNTI
GFFAPHNAYSSSGDRGQQVTEFKAMVKAFHAAGLEVILDVVYNHTAEAGTTGPTLSFRGLDDRGFYYRTGGDGHAEPFED
TYWDVTGCGNTVDASNPFALRLILDSLRYWVTEMHVDGFRFDLMSALTRVHRQIDMRSHLLTAIGQDPVLRHVKLIAEPW
DASMDGYRVGEFPPPWVEWNDQYRDEIRDFWRNHTSGIRGVATRLAGSSDLYLDDGRSPYASVNFVTAHDGFTMRDLVTY
ERKRNEANGEDNRDGTDNNRSWNHGVEGESHDPALVAIRRRQVANMMATLCLSNGVPMITAGDERGRTQGGNNNAYAQDN
ETSWIDWRPDDAWLDVYEVTKTALRLRREHPALRQRHWFEGRPTLIGGPKDLAWLHPSGREMSGDDWHDPTLRAVGMFVS
GAPLRAPGPRGEQQADKSFVLWFNSDWLPQRVTLPENDWVRAGEVVLSTDFRLPVGTPVKAGDQVHIGRRTVVVFRET

Sequences:

>Translated_718_residues
MTPGLWRDRGERAPVWPGRSWPLGSTWSAEATNFAVHAPRATAAWVCLFDEEGRERRHPLTEQELGIWHGAIPGVAPGTR
YGYRVDGPWDPAQGLRFNADKLLLDPFARAVSGDLTLDPAIFGYVEGAPDRRSDTDSAPYVPRSVVLGDDDFDWDDDAPP
RHPWQDSAIYEMHVKGMTALHDRVPEELRGTYAGLATPAVVDYLKDLGITAVELLPIHQFVSETSLLERGLVNYWGYNTI
GFFAPHNAYSSSGDRGQQVTEFKAMVKAFHAAGLEVILDVVYNHTAEAGTTGPTLSFRGLDDRGFYYRTGGDGHAEPFED
TYWDVTGCGNTVDASNPFALRLILDSLRYWVTEMHVDGFRFDLMSALTRVHRQIDMRSHLLTAIGQDPVLRHVKLIAEPW
DASMDGYRVGEFPPPWVEWNDQYRDEIRDFWRNHTSGIRGVATRLAGSSDLYLDDGRSPYASVNFVTAHDGFTMRDLVTY
ERKRNEANGEDNRDGTDNNRSWNHGVEGESHDPALVAIRRRQVANMMATLCLSNGVPMITAGDERGRTQGGNNNAYAQDN
ETSWIDWRPDDAWLDVYEVTKTALRLRREHPALRQRHWFEGRPTLIGGPKDLAWLHPSGREMSGDDWHDPTLRAVGMFVS
GAPLRAPGPRGEQQADKSFVLWFNSDWLPQRVTLPENDWVRAGEVVLSTDFRLPVGTPVKAGDQVHIGRRTVVVFRET
>Mature_717_residues
TPGLWRDRGERAPVWPGRSWPLGSTWSAEATNFAVHAPRATAAWVCLFDEEGRERRHPLTEQELGIWHGAIPGVAPGTRY
GYRVDGPWDPAQGLRFNADKLLLDPFARAVSGDLTLDPAIFGYVEGAPDRRSDTDSAPYVPRSVVLGDDDFDWDDDAPPR
HPWQDSAIYEMHVKGMTALHDRVPEELRGTYAGLATPAVVDYLKDLGITAVELLPIHQFVSETSLLERGLVNYWGYNTIG
FFAPHNAYSSSGDRGQQVTEFKAMVKAFHAAGLEVILDVVYNHTAEAGTTGPTLSFRGLDDRGFYYRTGGDGHAEPFEDT
YWDVTGCGNTVDASNPFALRLILDSLRYWVTEMHVDGFRFDLMSALTRVHRQIDMRSHLLTAIGQDPVLRHVKLIAEPWD
ASMDGYRVGEFPPPWVEWNDQYRDEIRDFWRNHTSGIRGVATRLAGSSDLYLDDGRSPYASVNFVTAHDGFTMRDLVTYE
RKRNEANGEDNRDGTDNNRSWNHGVEGESHDPALVAIRRRQVANMMATLCLSNGVPMITAGDERGRTQGGNNNAYAQDNE
TSWIDWRPDDAWLDVYEVTKTALRLRREHPALRQRHWFEGRPTLIGGPKDLAWLHPSGREMSGDDWHDPTLRAVGMFVSG
APLRAPGPRGEQQADKSFVLWFNSDWLPQRVTLPENDWVRAGEVVLSTDFRLPVGTPVKAGDQVHIGRRTVVVFRET

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=665, Percent_Identity=44.0601503759398, Blast_Score=540, Evalue=1e-154,
Organism=Escherichia coli, GI1789839, Length=339, Percent_Identity=25.6637168141593, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80732; Mature: 80600

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGLWRDRGERAPVWPGRSWPLGSTWSAEATNFAVHAPRATAAWVCLFDEEGRERRHPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCCC
TEQELGIWHGAIPGVAPGTRYGYRVDGPWDPAQGLRFNADKLLLDPFARAVSGDLTLDPA
CHHHCCHHHCCCCCCCCCCCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHCCCCEECCH
IFGYVEGAPDRRSDTDSAPYVPRSVVLGDDDFDWDDDAPPRHPWQDSAIYEMHVKGMTAL
HEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
HDRVPEELRGTYAGLATPAVVDYLKDLGITAVELLPIHQFVSETSLLERGLVNYWGYNTI
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCE
GFFAPHNAYSSSGDRGQQVTEFKAMVKAFHAAGLEVILDVVYNHTAEAGTTGPTLSFRGL
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
DDRGFYYRTGGDGHAEPFEDTYWDVTGCGNTVDASNPFALRLILDSLRYWVTEMHVDGFR
CCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
FDLMSALTRVHRQIDMRSHLLTAIGQDPVLRHVKLIAEPWDASMDGYRVGEFPPPWVEWN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCC
DQYRDEIRDFWRNHTSGIRGVATRLAGSSDLYLDDGRSPYASVNFVTAHDGFTMRDLVTY
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHH
ERKRNEANGEDNRDGTDNNRSWNHGVEGESHDPALVAIRRRQVANMMATLCLSNGVPMIT
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
AGDERGRTQGGNNNAYAQDNETSWIDWRPDDAWLDVYEVTKTALRLRREHPALRQRHWFE
ECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
GRPTLIGGPKDLAWLHPSGREMSGDDWHDPTLRAVGMFVSGAPLRAPGPRGEQQADKSFV
CCCCEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
LWFNSDWLPQRVTLPENDWVRAGEVVLSTDFRLPVGTPVKAGDQVHIGRRTVVVFRET
EEECCCCCCCEECCCCCCCEECCEEEEEECCCCCCCCCCCCCCEEEECCEEEEEEECC
>Mature Secondary Structure 
TPGLWRDRGERAPVWPGRSWPLGSTWSAEATNFAVHAPRATAAWVCLFDEEGRERRHPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCCC
TEQELGIWHGAIPGVAPGTRYGYRVDGPWDPAQGLRFNADKLLLDPFARAVSGDLTLDPA
CHHHCCHHHCCCCCCCCCCCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHCCCCEECCH
IFGYVEGAPDRRSDTDSAPYVPRSVVLGDDDFDWDDDAPPRHPWQDSAIYEMHVKGMTAL
HEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
HDRVPEELRGTYAGLATPAVVDYLKDLGITAVELLPIHQFVSETSLLERGLVNYWGYNTI
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCE
GFFAPHNAYSSSGDRGQQVTEFKAMVKAFHAAGLEVILDVVYNHTAEAGTTGPTLSFRGL
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
DDRGFYYRTGGDGHAEPFEDTYWDVTGCGNTVDASNPFALRLILDSLRYWVTEMHVDGFR
CCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
FDLMSALTRVHRQIDMRSHLLTAIGQDPVLRHVKLIAEPWDASMDGYRVGEFPPPWVEWN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCC
DQYRDEIRDFWRNHTSGIRGVATRLAGSSDLYLDDGRSPYASVNFVTAHDGFTMRDLVTY
HHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHH
ERKRNEANGEDNRDGTDNNRSWNHGVEGESHDPALVAIRRRQVANMMATLCLSNGVPMIT
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
AGDERGRTQGGNNNAYAQDNETSWIDWRPDDAWLDVYEVTKTALRLRREHPALRQRHWFE
ECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCC
GRPTLIGGPKDLAWLHPSGREMSGDDWHDPTLRAVGMFVSGAPLRAPGPRGEQQADKSFV
CCCCEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
LWFNSDWLPQRVTLPENDWVRAGEVVLSTDFRLPVGTPVKAGDQVHIGRRTVVVFRET
EEECCCCCCCEECCCCCCCEECCEEEEEECCCCCCCCCCCCCCEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]