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The map label for this gene is yogA [H]

Identifier: 119716029

GI number: 119716029

Start: 1907738

End: 1909090

Strand: Reverse

Name: yogA [H]

Synonym: Noca_1795

Alternate gene names: 119716029

Gene position: 1909090-1907738 (Counterclockwise)

Preceding gene: 119716030

Following gene: 119716028

Centisome position: 38.29

GC content: 68.29

Gene sequence:

>1353_bases
GTGCAGCACATCCTCGACGCGATCATGGCCGCCTCCGACGACCCGCGGAGTGCCACCGAGGCCGACTTCGCCGGCCTCGA
CCTGCCCGAGTCCTACCGGGCGGTGACGGTGCACAAGGACGAGGTCGACATGTTCGACGGCCTCGCCACGAAGGAGAAGG
ACCCGCGCAAGTCGCTGCACGTCGAGGACGTGCCGCTGCCCGAGCTCGGCCCCGGCGAGGCCTACGTCGCGGTGATGGCG
TCGGCGATCAACTACAACACGGTGTGGACCTCGATCTTCGAGCCCGCCTCCACGTTCGGCTTCCTGGAGCGCTACGGGCG
GCTCTCGCCGCTGGCCAAGCGGCACGACCTGCCCTACCACGTGGTCGGTTCCGACCTCGCCGGCGTGGTGCTCGCCACCG
GGCCCGGCGTGCATCTGTGGAAGCCGGGCACCGAGGTCGTGGCGCACTGCCTCTCGGTCGAGCTGGAGCACCCCGACGGC
CACAGCGACACGATGCTCGACCCCGAGCAGCGGATCTGGGGGTTCGAGACGAACTTCGGCGGGCTGGCCCAGATCGCACT
GGTCAAGGCCAACCAGCTGATGCCCAAGCCCGCCCACCTGACCTGGGAGGAGGCCGCCTCCCCCGGTCTGGTCAACTGCA
CGGCGTACCGCCAGCTGGTCTCCAAGAACGGCGGCGACATGAAGCAGGGCGACAACGTGCTGATCTGGGGCGCCTCGGGC
GGCCTGGGCGGCTTCGCGACCCAGTACGCCCTCAACGGCGGCGCCAACCCGATCTGCGTGGTCTCGAGTGAGGAGAAGGC
CGAGATCTGCCGCAAGATGGGCGCCGAGCTGATCATCAACCGCAACGAGGAGGGCTACCGGTTCTGGAAGGACGAGCAGA
CCCAGGACCCGCGGGAGTGGAAGCGCTTCGGCTCCAAGATCCGCGAGCTCACCGGCGGTGAGGACATCGACATCGTCTTC
GAGCACCCCGGCCGCGAGACCTTCGGCGCGTCGGTCTACGTGACCCGCAAGGGCGGCACGATCACCACCTGCGCGTCGAC
CTCGGGCTACATGCACGAGTACGACAACCGCTACCTGTGGATGAGCCTCAAGAAGATCATCTCCTCCCACTTCGCCAACT
ACCGCGAGTCCTGGGAGGCCAACCGGCTGATCGCCAAGGGCAAGATCCACCCGACCCTGTCCAAGACCTACGCCCTCGAC
GACGTCGGCCAGGCCACCCTCGACGTCCACCAGAACCTCCACCAGGGCAAGGTCGGCGTGCTCTGCCTGGCCCCCGAGGA
GGGCCTCGGCGTCCGCGACCACGAGATGCGCGCGCGCCACCTCGAGGAGATCAACCGCTTCCGCGACCTGTGA

Upstream 100 bases:

>100_bases
TTTCGCACTGAGGAACACATCACAGGGGGTGTTACCCCCTGTTACCCGTGGGTAAGGTCCGGCCATCCCCCGACGCGAAC
CGACCTCACCAGGAGCCGAT

Downstream 100 bases:

>100_bases
GCTGCCCGCGCCTGAGCTCGTGCAGGCTCGGACGGCAGCAGCCGGGTCGAGCAGCCGCGTGACCGACGGACGAGGTCGCG
ACCGGCGCACCGAGACCGAG

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MQHILDAIMAASDDPRSATEADFAGLDLPESYRAVTVHKDEVDMFDGLATKEKDPRKSLHVEDVPLPELGPGEAYVAVMA
SAINYNTVWTSIFEPASTFGFLERYGRLSPLAKRHDLPYHVVGSDLAGVVLATGPGVHLWKPGTEVVAHCLSVELEHPDG
HSDTMLDPEQRIWGFETNFGGLAQIALVKANQLMPKPAHLTWEEAASPGLVNCTAYRQLVSKNGGDMKQGDNVLIWGASG
GLGGFATQYALNGGANPICVVSSEEKAEICRKMGAELIINRNEEGYRFWKDEQTQDPREWKRFGSKIRELTGGEDIDIVF
EHPGRETFGASVYVTRKGGTITTCASTSGYMHEYDNRYLWMSLKKIISSHFANYRESWEANRLIAKGKIHPTLSKTYALD
DVGQATLDVHQNLHQGKVGVLCLAPEEGLGVRDHEMRARHLEEINRFRDL

Sequences:

>Translated_450_residues
MQHILDAIMAASDDPRSATEADFAGLDLPESYRAVTVHKDEVDMFDGLATKEKDPRKSLHVEDVPLPELGPGEAYVAVMA
SAINYNTVWTSIFEPASTFGFLERYGRLSPLAKRHDLPYHVVGSDLAGVVLATGPGVHLWKPGTEVVAHCLSVELEHPDG
HSDTMLDPEQRIWGFETNFGGLAQIALVKANQLMPKPAHLTWEEAASPGLVNCTAYRQLVSKNGGDMKQGDNVLIWGASG
GLGGFATQYALNGGANPICVVSSEEKAEICRKMGAELIINRNEEGYRFWKDEQTQDPREWKRFGSKIRELTGGEDIDIVF
EHPGRETFGASVYVTRKGGTITTCASTSGYMHEYDNRYLWMSLKKIISSHFANYRESWEANRLIAKGKIHPTLSKTYALD
DVGQATLDVHQNLHQGKVGVLCLAPEEGLGVRDHEMRARHLEEINRFRDL
>Mature_450_residues
MQHILDAIMAASDDPRSATEADFAGLDLPESYRAVTVHKDEVDMFDGLATKEKDPRKSLHVEDVPLPELGPGEAYVAVMA
SAINYNTVWTSIFEPASTFGFLERYGRLSPLAKRHDLPYHVVGSDLAGVVLATGPGVHLWKPGTEVVAHCLSVELEHPDG
HSDTMLDPEQRIWGFETNFGGLAQIALVKANQLMPKPAHLTWEEAASPGLVNCTAYRQLVSKNGGDMKQGDNVLIWGASG
GLGGFATQYALNGGANPICVVSSEEKAEICRKMGAELIINRNEEGYRFWKDEQTQDPREWKRFGSKIRELTGGEDIDIVF
EHPGRETFGASVYVTRKGGTITTCASTSGYMHEYDNRYLWMSLKKIISSHFANYRESWEANRLIAKGKIHPTLSKTYALD
DVGQATLDVHQNLHQGKVGVLCLAPEEGLGVRDHEMRARHLEEINRFRDL

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=321, Percent_Identity=24.2990654205607, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI13236495, Length=321, Percent_Identity=24.2990654205607, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790485, Length=316, Percent_Identity=25.3164556962025, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=401, Percent_Identity=21.4463840399002, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323729, Length=369, Percent_Identity=24.6612466124661, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 49832; Mature: 49832

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHILDAIMAASDDPRSATEADFAGLDLPESYRAVTVHKDEVDMFDGLATKEKDPRKSLH
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCCCCHHCCC
VEDVPLPELGPGEAYVAVMASAINYNTVWTSIFEPASTFGFLERYGRLSPLAKRHDLPYH
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHCCCCCHHHHCCCCEE
VVGSDLAGVVLATGPGVHLWKPGTEVVAHCLSVELEHPDGHSDTMLDPEQRIWGFETNFG
EECCCCCEEEEECCCCCEEECCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHEEECCCCC
GLAQIALVKANQLMPKPAHLTWEEAASPGLVNCTAYRQLVSKNGGDMKQGDNVLIWGASG
CCEEEEEEHHHHCCCCCCCCCHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCEEEEECCC
GLGGFATQYALNGGANPICVVSSEEKAEICRKMGAELIINRNEEGYRFWKDEQTQDPREW
CCCCHHHHHEECCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCHHHH
KRFGSKIRELTGGEDIDIVFEHPGRETFGASVYVTRKGGTITTCASTSGYMHEYDNRYLW
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHCCCEEH
MSLKKIISSHFANYRESWEANRLIAKGKIHPTLSKTYALDDVGQATLDVHQNLHQGKVGV
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHEEHHHHCHHHHHHHHHHCCCCEEE
LCLAPEEGLGVRDHEMRARHLEEINRFRDL
EEECCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHILDAIMAASDDPRSATEADFAGLDLPESYRAVTVHKDEVDMFDGLATKEKDPRKSLH
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCCCCHHCCC
VEDVPLPELGPGEAYVAVMASAINYNTVWTSIFEPASTFGFLERYGRLSPLAKRHDLPYH
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHCCCCCHHHHCCCCEE
VVGSDLAGVVLATGPGVHLWKPGTEVVAHCLSVELEHPDGHSDTMLDPEQRIWGFETNFG
EECCCCCEEEEECCCCCEEECCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHEEECCCCC
GLAQIALVKANQLMPKPAHLTWEEAASPGLVNCTAYRQLVSKNGGDMKQGDNVLIWGASG
CCEEEEEEHHHHCCCCCCCCCHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCEEEEECCC
GLGGFATQYALNGGANPICVVSSEEKAEICRKMGAELIINRNEEGYRFWKDEQTQDPREW
CCCCHHHHHEECCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCHHHH
KRFGSKIRELTGGEDIDIVFEHPGRETFGASVYVTRKGGTITTCASTSGYMHEYDNRYLW
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHCCCEEH
MSLKKIISSHFANYRESWEANRLIAKGKIHPTLSKTYALDDVGQATLDVHQNLHQGKVGV
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHEEHHHHCHHHHHHHHHHCCCCEEE
LCLAPEEGLGVRDHEMRARHLEEINRFRDL
EEECCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]