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The map label for this gene is 119715904

Identifier: 119715904

GI number: 119715904

Start: 1774808

End: 1776280

Strand: Direct

Name: 119715904

Synonym: Noca_1669

Alternate gene names: NA

Gene position: 1774808-1776280 (Clockwise)

Preceding gene: 119715903

Following gene: 119715908

Centisome position: 35.6

GC content: 72.17

Gene sequence:

>1473_bases
ATGGGTGGACGACAGCGGTTGAGCGGCCAGGACGCGCTCTGGTTGGCGATGGACAAGCCCGGCAACCTGATGGTCGTCGA
CAGTCTCTTCTGGACGGCCGAGCCGATCGACTGGGACCGCTTCCGTGAGGTGATGAGGGAGCGCTTCTGGGAACGGTACG
ACGTGGTCCGCAGCGTCATCGTGCGCGACGAGGACGGCGCCCTGTGCTGGGAGGAGGTGCCCGAGGCCGACCTCGACGAC
CGCTTCGAGCAGGTGGTGCTCCCGGCGCCCGGGGGTGACGCGGAGCTGCAGGACCTGATCGCCGCGCAGCGGGTGCTCCC
GCTCGATCGCGGCGAGCCGCTGTGGCGTGCGGTGCTCGTGGACGGGTTCCACGGCGGCAGCGCCGTGCTGTTCCGGGGCC
ACCACTCGATCGCCGACGGGATCCGGATGGTCCAGCTCGTGCTGCGGGTCTTCGACTGCAGCCCCGACGGCGAGGACCCC
GGCCCAGCGCGGAAGACGGCCAGGAAGACGGTGCGGAAGGCGCCTGATGCCGCTCGGACGCCGGTCCCGCGCCGGGGCGA
CACCTCCCTGACCGGCCGTGCGGTGGCCGCTGCGACCACCTCCCTGCAGGTCGCCCGGAGCGCGATGACCAACCCGGTAG
GGGCCGCGCACAGCGCCCTCACGCTCTCCGAGGCCATGCTCGGCCGGTTCGGTGCCCTGCCGGTGGTCTCGGCGCTCCCC
GGCGACGTCGACGCTGCTCGCAAGCTGGTCCTGGGGACCCGCAACGACACCACCGCGTGGAGCGGGACGGTGGGGGACCG
CAAGGCGATCGCCTGGACCGCTCCGTTGTCCCTCGCCGAGGTCAAGGCGGTCGCGCACGCGCACGGGGCGACCGCGAACG
ACGTGCTGGTCAGCTGCGTCGCGCAGTCGCTGCGGGCGTACCTCGAGGCCCATGACGCGGTGTGCCACAGCGTCACGTGG
GACGTGCCGGTCAACCTCAAGCCGTTCGACCCGGACCTGCCCGTCGAGCTCGGCAACGGGTTCGCGCTGGTGCAGCTCGA
GCTGCCGACGAACATCGACGACCCGGTCCGCGCCCTCGACGTGGTGCGGCGCCGGATGAGCCGGATCAAGAACGGCCACG
AGGCGGTCGTCGACTACGGGATCCAGGCCGCCATCGGTCGGATGAGCACGGCCCTCTACCGCGCGACCATCGACCTCCTG
GCCAACCGGGCGGTCGGAGTGCTGACGAACGTGCCGGGGCCGCAGGTGCCGCTCTACATCGCCGGGCGGAAGGTCGAGGC
GATGCTGGGCTGGGCGCCGCTGACGGCCGACCAGGCGATGAGCCTGACGATCTACAGCTACGACGGCAAGGTGTTCGTCG
GCCTCGCCGCCGACGCCGGCCTGGTGCCGGACCACCAGCAGGTGGTCGACGGTTTCGCCCAGGCGTTCGCGCGCCTGGTC
GAGCGGACCGAGGCGACCCGGCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
GTGACGCTCGGCGCGCGCACTGATGACGTTCGTCCCTGCCCGTCGACCGCGGAGGGCGTGACGATGGGCGCTACCAGCGG
GTCATGAGGGTGAGGCGGTC

Downstream 100 bases:

>100_bases
GCCCGGGGCCGAGGCGAGGGCCGCGCCGTCACCCGGCGCGCTGCAGCGTGCGGGTCAGCTGGTCGACGGCGCGGTCGACC
TCCGCCTCGATCCGGCCGGC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1760/MT1809 [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MGGRQRLSGQDALWLAMDKPGNLMVVDSLFWTAEPIDWDRFREVMRERFWERYDVVRSVIVRDEDGALCWEEVPEADLDD
RFEQVVLPAPGGDAELQDLIAAQRVLPLDRGEPLWRAVLVDGFHGGSAVLFRGHHSIADGIRMVQLVLRVFDCSPDGEDP
GPARKTARKTVRKAPDAARTPVPRRGDTSLTGRAVAAATTSLQVARSAMTNPVGAAHSALTLSEAMLGRFGALPVVSALP
GDVDAARKLVLGTRNDTTAWSGTVGDRKAIAWTAPLSLAEVKAVAHAHGATANDVLVSCVAQSLRAYLEAHDAVCHSVTW
DVPVNLKPFDPDLPVELGNGFALVQLELPTNIDDPVRALDVVRRRMSRIKNGHEAVVDYGIQAAIGRMSTALYRATIDLL
ANRAVGVLTNVPGPQVPLYIAGRKVEAMLGWAPLTADQAMSLTIYSYDGKVFVGLAADAGLVPDHQQVVDGFAQAFARLV
ERTEATRRAG

Sequences:

>Translated_490_residues
MGGRQRLSGQDALWLAMDKPGNLMVVDSLFWTAEPIDWDRFREVMRERFWERYDVVRSVIVRDEDGALCWEEVPEADLDD
RFEQVVLPAPGGDAELQDLIAAQRVLPLDRGEPLWRAVLVDGFHGGSAVLFRGHHSIADGIRMVQLVLRVFDCSPDGEDP
GPARKTARKTVRKAPDAARTPVPRRGDTSLTGRAVAAATTSLQVARSAMTNPVGAAHSALTLSEAMLGRFGALPVVSALP
GDVDAARKLVLGTRNDTTAWSGTVGDRKAIAWTAPLSLAEVKAVAHAHGATANDVLVSCVAQSLRAYLEAHDAVCHSVTW
DVPVNLKPFDPDLPVELGNGFALVQLELPTNIDDPVRALDVVRRRMSRIKNGHEAVVDYGIQAAIGRMSTALYRATIDLL
ANRAVGVLTNVPGPQVPLYIAGRKVEAMLGWAPLTADQAMSLTIYSYDGKVFVGLAADAGLVPDHQQVVDGFAQAFARLV
ERTEATRRAG
>Mature_489_residues
GGRQRLSGQDALWLAMDKPGNLMVVDSLFWTAEPIDWDRFREVMRERFWERYDVVRSVIVRDEDGALCWEEVPEADLDDR
FEQVVLPAPGGDAELQDLIAAQRVLPLDRGEPLWRAVLVDGFHGGSAVLFRGHHSIADGIRMVQLVLRVFDCSPDGEDPG
PARKTARKTVRKAPDAARTPVPRRGDTSLTGRAVAAATTSLQVARSAMTNPVGAAHSALTLSEAMLGRFGALPVVSALPG
DVDAARKLVLGTRNDTTAWSGTVGDRKAIAWTAPLSLAEVKAVAHAHGATANDVLVSCVAQSLRAYLEAHDAVCHSVTWD
VPVNLKPFDPDLPVELGNGFALVQLELPTNIDDPVRALDVVRRRMSRIKNGHEAVVDYGIQAAIGRMSTALYRATIDLLA
NRAVGVLTNVPGPQVPLYIAGRKVEAMLGWAPLTADQAMSLTIYSYDGKVFVGLAADAGLVPDHQQVVDGFAQAFARLVE
RTEATRRAG

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 52943; Mature: 52812

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGRQRLSGQDALWLAMDKPGNLMVVDSLFWTAEPIDWDRFREVMRERFWERYDVVRSVI
CCCCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VRDEDGALCWEEVPEADLDDRFEQVVLPAPGGDAELQDLIAAQRVLPLDRGEPLWRAVLV
EECCCCCCCHHHCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHEEEHEE
DGFHGGSAVLFRGHHSIADGIRMVQLVLRVFDCSPDGEDPGPARKTARKTVRKAPDAART
ECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PVPRRGDTSLTGRAVAAATTSLQVARSAMTNPVGAAHSALTLSEAMLGRFGALPVVSALP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCC
GDVDAARKLVLGTRNDTTAWSGTVGDRKAIAWTAPLSLAEVKAVAHAHGATANDVLVSCV
CCHHHHHHHHEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHH
AQSLRAYLEAHDAVCHSVTWDVPVNLKPFDPDLPVELGNGFALVQLELPTNIDDPVRALD
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHH
VVRRRMSRIKNGHEAVVDYGIQAAIGRMSTALYRATIDLLANRAVGVLTNVPGPQVPLYI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEE
AGRKVEAMLGWAPLTADQAMSLTIYSYDGKVFVGLAADAGLVPDHQQVVDGFAQAFARLV
CCCEEEHHHCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ERTEATRRAG
HHHHHHHCCC
>Mature Secondary Structure 
GGRQRLSGQDALWLAMDKPGNLMVVDSLFWTAEPIDWDRFREVMRERFWERYDVVRSVI
CCCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VRDEDGALCWEEVPEADLDDRFEQVVLPAPGGDAELQDLIAAQRVLPLDRGEPLWRAVLV
EECCCCCCCHHHCCCCCCCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHEEEHEE
DGFHGGSAVLFRGHHSIADGIRMVQLVLRVFDCSPDGEDPGPARKTARKTVRKAPDAART
ECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PVPRRGDTSLTGRAVAAATTSLQVARSAMTNPVGAAHSALTLSEAMLGRFGALPVVSALP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCC
GDVDAARKLVLGTRNDTTAWSGTVGDRKAIAWTAPLSLAEVKAVAHAHGATANDVLVSCV
CCHHHHHHHHEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHH
AQSLRAYLEAHDAVCHSVTWDVPVNLKPFDPDLPVELGNGFALVQLELPTNIDDPVRALD
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHH
VVRRRMSRIKNGHEAVVDYGIQAAIGRMSTALYRATIDLLANRAVGVLTNVPGPQVPLYI
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEE
AGRKVEAMLGWAPLTADQAMSLTIYSYDGKVFVGLAADAGLVPDHQQVVDGFAQAFARLV
CCCEEEHHHCCCCCCCCCCEEEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ERTEATRRAG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]