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The map label for this gene is hyuB [H]

Identifier: 119715733

GI number: 119715733

Start: 1588064

End: 1590043

Strand: Direct

Name: hyuB [H]

Synonym: Noca_1497

Alternate gene names: 119715733

Gene position: 1588064-1590043 (Clockwise)

Preceding gene: 119715732

Following gene: 119715734

Centisome position: 31.85

GC content: 72.98

Gene sequence:

>1980_bases
ATGCGCGCACGGAGCGAAGCGAGTACGTGCATGAGCCGCCGCGCACCCACCCAGTTCCCGTTCGGCCACCTGACCGCCGA
CGCGGGCGCGAGCGCCGACCCCGTGCTGGTCGAGATCGTCCAGGGCTCCCTGGCGAGTGTGGAGATGGAGGTCGAGACCG
CCATCGGCCGCACCAGCCGGAGCCCGATGATCCGCGACGCGCACGACTTCCGCGCCGGCATCCACGACCGGCTCCTGCGC
AAGCTCACCGGCCGGTCCTACTCCGCGCTGGTGCACCCGGTCGCCCGCGACTTCCCGATCGAGGAGATGCGCGAGGGGGA
CGTCTTCTTCCACAACGACGTCTACCGCTCCGAGGGCGGGATCGGGCACCTGCCGGACCTGTGCGTGACGGTCCCCGTGT
TCGCCAAGGACGCAGGGGCCTCCGGGGGGTCCACGGGGGGCGGAGCCCCCCGTGTGGTGGCGTTCGTGCAGGCCTTCGGC
CACCACGACGACATCGGCGGCGCGGTCCCCGGCTCGATGCCCTCCCACGCGACCAGCGTGTTCGAGGAGGGGCTGATGGT
CCCGCCGATCCGGCTCTGGGACGCCGGCGTCCCGAACCGCTCGGCGCTGGCGATCATGACCCGCAACTCCCGGATGCCCG
AGTCCCTCGCGGCCGACCTGGACGCCGAGTGCTCGGCCTGCCTGATGGGTGCCCGCCGGCTCGGCGAGCTCTTCGACCGG
TACGGCCTCGACGCGGTCGAGTCCTGCTTCGACGCGATCATCAACCGCACCACCGAGACCTACCGCCGCGAGATCCTCGG
CAAGATCCCGGTCGGCTCCTGGGTCTGGGAGGACTACGCCGAGCACGACGGCGTCGACGAGCCGCAGCTGCACACCCAGC
GGATCACGCTCACCCGGACGCCCGCGGACGACCCCGAGGGCGAGCGGCTGATCCTCGACTTCGACGGCACCTCGCCGCAG
GCCAAGGGCCCGATCAACCACTGCGGCGACTACAGCGACGGCGTCTTCCTCAAGAAGTGGCTGGCGCCGATCCTGCGCAA
CCTCGCCGACACCCCCGAGCGGATGGCCGAGCTCGACGTCAACGAGGGCATCGTGCCGCTCATCGAGATGCGCTTCCCCG
AGCCCGGCACCCTGCTCACGCCGGTGTTCCCGGCGCCCACCAACGCGCGCACCTTCGTGATCCTGCGGCTCCTGGGCGTG
CTCGCCGGCGTGGTCGCCAAGGCCGTCGACGGCCGGATGCCGGCCGACCAGGAGACGATCCGCTACACCGGCGTGTACGG
CGAGGACCTCGAGGGCCGTCCGTACCTCATGCGCGAGGTGCTCGGCGGCGGCTCCGGCGGCCGCTACTACGCGGACGGCG
AGGACACCATCCACGTGGTGCCGGACTCCCGGAACCTGCCGACCGAGTTCACCGAGGCCCGGTTCCCGTTCCGGGTCGAG
TCGCTCTCGCTCGCCGTGGACAGCGGCGGCGCCGGGCAGTACCGCGGCGGCCTGGGCTACGAGAAGCACCTGCGGATGCT
CAAGGACGGCCACTTCATGTCGATCGCCGACCGCTCGATCCTCGCCTGCTGGGGCGTCAAGGGCGGCCGGGCCGGCCAGC
CGTTCCAGGTCACCATCGACCCCGGCGGGCCCAACGAGCGTGAGGTCGACGCCCTCGCCGACGCCGAGCCGGTGGCCGCC
GGCGAGGTGATCCGGATCCGGACCACCGGCGGCGGAGGCTGGGGCGACCCGTTGCGGCGCGACCCCGCCCTCGTGGTCCG
CGACGTCGTGTGGCGCAAGGTCTCACCCGAGGCCGCGCTGGGCGACTACGGCGTGGTGCTGACCGGCTCCCTCGACGACG
ACTCGCTGGGGTACGACGAGGCCGCGACCGCCGCCGAGCGGGCCGCCCGGCCCGCGGCGAGCGAGGCGTTCTTCGACCGC
GGGCCCGGCTACGCCACGCTGGCCGCGGGCGCGCCGTACGCCGCGGTCGACCTGGTCTGA

Upstream 100 bases:

>100_bases
ACGGCCCGCATCGACGAGTACCTCAACATCATCGTGACGAGGAGCCAGGCATGAGCGAGCGCAGCGAGCGAATCATCCAA
CTCAGCGCAGCGTGTGCCTC

Downstream 100 bases:

>100_bases
CCGGCGCCGACGAGCGCCCGCCGGTTCTCATCCCGGTGGCCTTGTCCGCCGCACCCTGCGGGCGCACCATGGAAGGGACG
GCACCCCGAAGAGGTGAGGC

Product: hydantoinase B/oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MRARSEASTCMSRRAPTQFPFGHLTADAGASADPVLVEIVQGSLASVEMEVETAIGRTSRSPMIRDAHDFRAGIHDRLLR
KLTGRSYSALVHPVARDFPIEEMREGDVFFHNDVYRSEGGIGHLPDLCVTVPVFAKDAGASGGSTGGGAPRVVAFVQAFG
HHDDIGGAVPGSMPSHATSVFEEGLMVPPIRLWDAGVPNRSALAIMTRNSRMPESLAADLDAECSACLMGARRLGELFDR
YGLDAVESCFDAIINRTTETYRREILGKIPVGSWVWEDYAEHDGVDEPQLHTQRITLTRTPADDPEGERLILDFDGTSPQ
AKGPINHCGDYSDGVFLKKWLAPILRNLADTPERMAELDVNEGIVPLIEMRFPEPGTLLTPVFPAPTNARTFVILRLLGV
LAGVVAKAVDGRMPADQETIRYTGVYGEDLEGRPYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLPTEFTEARFPFRVE
SLSLAVDSGGAGQYRGGLGYEKHLRMLKDGHFMSIADRSILACWGVKGGRAGQPFQVTIDPGGPNEREVDALADAEPVAA
GEVIRIRTTGGGGWGDPLRRDPALVVRDVVWRKVSPEAALGDYGVVLTGSLDDDSLGYDEAATAAERAARPAASEAFFDR
GPGYATLAAGAPYAAVDLV

Sequences:

>Translated_659_residues
MRARSEASTCMSRRAPTQFPFGHLTADAGASADPVLVEIVQGSLASVEMEVETAIGRTSRSPMIRDAHDFRAGIHDRLLR
KLTGRSYSALVHPVARDFPIEEMREGDVFFHNDVYRSEGGIGHLPDLCVTVPVFAKDAGASGGSTGGGAPRVVAFVQAFG
HHDDIGGAVPGSMPSHATSVFEEGLMVPPIRLWDAGVPNRSALAIMTRNSRMPESLAADLDAECSACLMGARRLGELFDR
YGLDAVESCFDAIINRTTETYRREILGKIPVGSWVWEDYAEHDGVDEPQLHTQRITLTRTPADDPEGERLILDFDGTSPQ
AKGPINHCGDYSDGVFLKKWLAPILRNLADTPERMAELDVNEGIVPLIEMRFPEPGTLLTPVFPAPTNARTFVILRLLGV
LAGVVAKAVDGRMPADQETIRYTGVYGEDLEGRPYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLPTEFTEARFPFRVE
SLSLAVDSGGAGQYRGGLGYEKHLRMLKDGHFMSIADRSILACWGVKGGRAGQPFQVTIDPGGPNEREVDALADAEPVAA
GEVIRIRTTGGGGWGDPLRRDPALVVRDVVWRKVSPEAALGDYGVVLTGSLDDDSLGYDEAATAAERAARPAASEAFFDR
GPGYATLAAGAPYAAVDLV
>Mature_659_residues
MRARSEASTCMSRRAPTQFPFGHLTADAGASADPVLVEIVQGSLASVEMEVETAIGRTSRSPMIRDAHDFRAGIHDRLLR
KLTGRSYSALVHPVARDFPIEEMREGDVFFHNDVYRSEGGIGHLPDLCVTVPVFAKDAGASGGSTGGGAPRVVAFVQAFG
HHDDIGGAVPGSMPSHATSVFEEGLMVPPIRLWDAGVPNRSALAIMTRNSRMPESLAADLDAECSACLMGARRLGELFDR
YGLDAVESCFDAIINRTTETYRREILGKIPVGSWVWEDYAEHDGVDEPQLHTQRITLTRTPADDPEGERLILDFDGTSPQ
AKGPINHCGDYSDGVFLKKWLAPILRNLADTPERMAELDVNEGIVPLIEMRFPEPGTLLTPVFPAPTNARTFVILRLLGV
LAGVVAKAVDGRMPADQETIRYTGVYGEDLEGRPYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLPTEFTEARFPFRVE
SLSLAVDSGGAGQYRGGLGYEKHLRMLKDGHFMSIADRSILACWGVKGGRAGQPFQVTIDPGGPNEREVDALADAEPVAA
GEVIRIRTTGGGGWGDPLRRDPALVVRDVVWRKVSPEAALGDYGVVLTGSLDDDSLGYDEAATAAERAARPAASEAFFDR
GPGYATLAAGAPYAAVDLV

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=580, Percent_Identity=25.6896551724138, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI133901900, Length=572, Percent_Identity=27.7972027972028, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI133901902, Length=489, Percent_Identity=29.4478527607362, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6322634, Length=587, Percent_Identity=25.0425894378194, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI45550492, Length=594, Percent_Identity=25.9259259259259, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 70913; Mature: 70913

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARSEASTCMSRRAPTQFPFGHLTADAGASADPVLVEIVQGSLASVEMEVETAIGRTSR
CCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCHHHHHHHCCHHHEEEEHHHHHCCCCC
SPMIRDAHDFRAGIHDRLLRKLTGRSYSALVHPVARDFPIEEMREGDVFFHNDVYRSEGG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCEEEECCEECCCCC
IGHLPDLCVTVPVFAKDAGASGGSTGGGAPRVVAFVQAFGHHDDIGGAVPGSMPSHATSV
CCCCCHHHEEECHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
FEEGLMVPPIRLWDAGVPNRSALAIMTRNSRMPESLAADLDAECSACLMGARRLGELFDR
HHCCCCCCCHHHCCCCCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
YGLDAVESCFDAIINRTTETYRREILGKIPVGSWVWEDYAEHDGVDEPQLHTQRITLTRT
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEC
PADDPEGERLILDFDGTSPQAKGPINHCGDYSDGVFLKKWLAPILRNLADTPERMAELDV
CCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCC
NEGIVPLIEMRFPEPGTLLTPVFPAPTNARTFVILRLLGVLAGVVAKAVDGRMPADQETI
CCCCEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
RYTGVYGEDLEGRPYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLPTEFTEARFPFRVE
EEECCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCHHHHCCCCEEEE
SLSLAVDSGGAGQYRGGLGYEKHLRMLKDGHFMSIADRSILACWGVKGGRAGQPFQVTID
EEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCEEEEEC
PGGPNEREVDALADAEPVAAGEVIRIRTTGGGGWGDPLRRDPALVVRDVVWRKVSPEAAL
CCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GDYGVVLTGSLDDDSLGYDEAATAAERAARPAASEAFFDRGPGYATLAAGAPYAAVDLV
CCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCCEEEECC
>Mature Secondary Structure
MRARSEASTCMSRRAPTQFPFGHLTADAGASADPVLVEIVQGSLASVEMEVETAIGRTSR
CCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCHHHHHHHCCHHHEEEEHHHHHCCCCC
SPMIRDAHDFRAGIHDRLLRKLTGRSYSALVHPVARDFPIEEMREGDVFFHNDVYRSEGG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCEEEECCEECCCCC
IGHLPDLCVTVPVFAKDAGASGGSTGGGAPRVVAFVQAFGHHDDIGGAVPGSMPSHATSV
CCCCCHHHEEECHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
FEEGLMVPPIRLWDAGVPNRSALAIMTRNSRMPESLAADLDAECSACLMGARRLGELFDR
HHCCCCCCCHHHCCCCCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
YGLDAVESCFDAIINRTTETYRREILGKIPVGSWVWEDYAEHDGVDEPQLHTQRITLTRT
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEC
PADDPEGERLILDFDGTSPQAKGPINHCGDYSDGVFLKKWLAPILRNLADTPERMAELDV
CCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCC
NEGIVPLIEMRFPEPGTLLTPVFPAPTNARTFVILRLLGVLAGVVAKAVDGRMPADQETI
CCCCEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
RYTGVYGEDLEGRPYLMREVLGGGSGGRYYADGEDTIHVVPDSRNLPTEFTEARFPFRVE
EEECCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCHHHHCCCCEEEE
SLSLAVDSGGAGQYRGGLGYEKHLRMLKDGHFMSIADRSILACWGVKGGRAGQPFQVTID
EEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCEEEEEC
PGGPNEREVDALADAEPVAAGEVIRIRTTGGGGWGDPLRRDPALVVRDVVWRKVSPEAAL
CCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GDYGVVLTGSLDDDSLGYDEAATAAERAARPAASEAFFDRGPGYATLAAGAPYAAVDLV
CCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]