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The map label for this gene is hyuA [H]

Identifier: 119715732

GI number: 119715732

Start: 1585897

End: 1588017

Strand: Direct

Name: hyuA [H]

Synonym: Noca_1496

Alternate gene names: 119715732

Gene position: 1585897-1588017 (Clockwise)

Preceding gene: 119715731

Following gene: 119715733

Centisome position: 31.81

GC content: 71.57

Gene sequence:

>2121_bases
ATGACCCGGAGCATCCGGATCGGCATCGACACCGGGGGCACGTTCACCGACGTTGTCGCGTTCGACGAGGACTCGGGGGA
GCTGGTCACCACCAAGACCCCGTCCACCCCGAGCAACCCGGCGGACGGCTTCCTGGCCGGCATCGACAAGGTGCTCGCCG
TGCTCGGCGCGAGCGGCGCCGACATCGACGCCGTCAGCCACGGCACCACCGTCGCCACCAACCAGCTGCTCGAGGGCAAG
GTGGACCGCCTGGGCTTCATCACCACCGCCGGCTACGAGGCGATGCTCGAGATCGCTCGCCAGTCGGTGCCCGACGGCTA
CGGCAACTCCTACTTCTGGGTCAAGCCGGACCGGATCGTCCCGCGTGACCGGGTCAAGGGCGTCGGGGGCCGGCTCGACT
TCACCGGGGCGGAGGTGCGGCCCTTCGACGAGGACGCCGCCCGCGCCGCGGCGCGGTGGTTCCGCGCCCAGGGGATCAAC
ACCCTCGGCGTCTGCTTCCTGCACGCCTACGCCAACCCCGAGCACGAGGAGCGGATGCGGGCCGTGCTCGCCGAGGAGCA
CCCCGACGCGGTGGTCTCCCTGTCGAGCGAGGTGCTGCGCGAGTACCGCGAGTACGAGCGCGCGATGACCACGCTGGTCG
ACGCGGCCGTCAAGCCGCGGCTCTCGGCGTACGTCTCCAACATCAAGGCGCGCCTCGCGGGCTACGTCCCGGCGGGTGCT
TCTGGCGGAGCCCCCCGTGTTGTCCCGTTCTACGTGATGAAGTCCAACGGCGGCGTGCTCTCGGCCGACGAGGTCGTGCA
CCAGCCGATCACCACCGTGCTCAGCGGCCCCGCCGCCGGCGCCCTCGGCGCCGCGCTGATCGCGAAGGTGGCCGGGTTCG
ACAAGGTCCTCACCTCCGACGGCGGCGGTACGTCGACCGACGTCAGCGTGGTGATCGACGGCGAGCCGACGCTGACCACC
GAGGGCTCGGTGGGGGCGTTCCCGTCCAAGATCCCGATGATCGACGTGGTCACCGTGGGCGCCGGCGGCGGCTCGATCGC
CTGGCTCTCGCCCGAGGGCACCCTCAAGGTCGGCCCGCAGTCGGCGGGCGCCGACCCGGGGCCGCTGTGCTACGGCAAGG
GCGGAACCGAGGTGACGATCACCGACGCCCACGTGGTCCTCGGCCGGATCCCCCCGCACCTGCTCGGCGGCGAGATCCCC
CTGGACGTCGACGCGGCACGCGCCGGCGTCGAGCAGCTCGCCGCGAAGCTCGGCCTCACGCCGGAGGCCTGCGCCACCGG
CATCCTCGAGATCTCCGCCTGGAACCAGGCCAACGCGCTGCGCCAGGTGACCGTCAAGCGCGGCCTGGACGTCCGCGACT
TCACGCTGACCACGTTCGGCGGCTCGGGCTCCCTGCTGCTGTGCCGGCTGATGGACGTCCTCGGGATCCCGACCGTGCTG
GTCCCGCCCAACCCGGGCAACGTCTCGGCGTTCGGCCTGCTCACCGTCGACGTCAAGAACGACTACGTGCAGACCCACGT
GACCCTCGACGACCACCTCGACCCGGCAGCGGTCACCGGCATCTACGACGAGCTCACCGCGCGCGCCGCCGAGGCGCTCG
CGAAGGAGGGCTTCGAGCCGGCGGAGCACCAGTTCGTGCGCACGGCCGACCTGCGCTACTTCGGCCAGGCCTTCGAGGTG
CGGGTACCGGTCGCCGCCGGCGCGCTCGACCCGCAGGCGGTGGCCGACGCGTTCCACGCCGAGCACAAGGCGCTCTACGG
CTACGACTTCTCCGGCGACTCCAGCCAGCAGGTCGAGTGGGTCAACCTCCGGGTCTCCGGGATCGGCCCGATCAAGCGGC
CGGCAATCACCCGCCACCCCGTCGAGTCGGGAGTTCTGGCGACCGACTCTCCCGACTCGACCGTCACCACTCCCGACTCG
ACGGTCCGGGCGGTGTGCTTCGACGCGGCCGAGGGGTACGTCGACACGCCGGTGCTCTGGCGTCCCGACCTCGCGCCGGG
GACGGTCCTGGACGGACCCGCGATCATCGAGGAGTTCGGCTCCACGGTGCCGCTCCACCCGGGCTTCACGGCCCGCATCG
ACGAGTACCTCAACATCATCGTGACGAGGAGCCAGGCATGA

Upstream 100 bases:

>100_bases
CCCCGGCGTACGTCCTCGCCTCGGGCCGGTTGGTCGCCAGCTCGAGCGCCACCACCACGTTCCACCTGGACAACCTGGAC
AACCTGGAAGGGAACCAGCC

Downstream 100 bases:

>100_bases
GCGAGCGCAGCGAGCGAATCATCCAACTCAGCGCAGCGTGTGCCTCATGCGCGCACGGAGCGAAGCGAGTACGTGCATGA
GCCGCCGCGCACCCACCCAG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MTRSIRIGIDTGGTFTDVVAFDEDSGELVTTKTPSTPSNPADGFLAGIDKVLAVLGASGADIDAVSHGTTVATNQLLEGK
VDRLGFITTAGYEAMLEIARQSVPDGYGNSYFWVKPDRIVPRDRVKGVGGRLDFTGAEVRPFDEDAARAAARWFRAQGIN
TLGVCFLHAYANPEHEERMRAVLAEEHPDAVVSLSSEVLREYREYERAMTTLVDAAVKPRLSAYVSNIKARLAGYVPAGA
SGGAPRVVPFYVMKSNGGVLSADEVVHQPITTVLSGPAAGALGAALIAKVAGFDKVLTSDGGGTSTDVSVVIDGEPTLTT
EGSVGAFPSKIPMIDVVTVGAGGGSIAWLSPEGTLKVGPQSAGADPGPLCYGKGGTEVTITDAHVVLGRIPPHLLGGEIP
LDVDAARAGVEQLAAKLGLTPEACATGILEISAWNQANALRQVTVKRGLDVRDFTLTTFGGSGSLLLCRLMDVLGIPTVL
VPPNPGNVSAFGLLTVDVKNDYVQTHVTLDDHLDPAAVTGIYDELTARAAEALAKEGFEPAEHQFVRTADLRYFGQAFEV
RVPVAAGALDPQAVADAFHAEHKALYGYDFSGDSSQQVEWVNLRVSGIGPIKRPAITRHPVESGVLATDSPDSTVTTPDS
TVRAVCFDAAEGYVDTPVLWRPDLAPGTVLDGPAIIEEFGSTVPLHPGFTARIDEYLNIIVTRSQA

Sequences:

>Translated_706_residues
MTRSIRIGIDTGGTFTDVVAFDEDSGELVTTKTPSTPSNPADGFLAGIDKVLAVLGASGADIDAVSHGTTVATNQLLEGK
VDRLGFITTAGYEAMLEIARQSVPDGYGNSYFWVKPDRIVPRDRVKGVGGRLDFTGAEVRPFDEDAARAAARWFRAQGIN
TLGVCFLHAYANPEHEERMRAVLAEEHPDAVVSLSSEVLREYREYERAMTTLVDAAVKPRLSAYVSNIKARLAGYVPAGA
SGGAPRVVPFYVMKSNGGVLSADEVVHQPITTVLSGPAAGALGAALIAKVAGFDKVLTSDGGGTSTDVSVVIDGEPTLTT
EGSVGAFPSKIPMIDVVTVGAGGGSIAWLSPEGTLKVGPQSAGADPGPLCYGKGGTEVTITDAHVVLGRIPPHLLGGEIP
LDVDAARAGVEQLAAKLGLTPEACATGILEISAWNQANALRQVTVKRGLDVRDFTLTTFGGSGSLLLCRLMDVLGIPTVL
VPPNPGNVSAFGLLTVDVKNDYVQTHVTLDDHLDPAAVTGIYDELTARAAEALAKEGFEPAEHQFVRTADLRYFGQAFEV
RVPVAAGALDPQAVADAFHAEHKALYGYDFSGDSSQQVEWVNLRVSGIGPIKRPAITRHPVESGVLATDSPDSTVTTPDS
TVRAVCFDAAEGYVDTPVLWRPDLAPGTVLDGPAIIEEFGSTVPLHPGFTARIDEYLNIIVTRSQA
>Mature_705_residues
TRSIRIGIDTGGTFTDVVAFDEDSGELVTTKTPSTPSNPADGFLAGIDKVLAVLGASGADIDAVSHGTTVATNQLLEGKV
DRLGFITTAGYEAMLEIARQSVPDGYGNSYFWVKPDRIVPRDRVKGVGGRLDFTGAEVRPFDEDAARAAARWFRAQGINT
LGVCFLHAYANPEHEERMRAVLAEEHPDAVVSLSSEVLREYREYERAMTTLVDAAVKPRLSAYVSNIKARLAGYVPAGAS
GGAPRVVPFYVMKSNGGVLSADEVVHQPITTVLSGPAAGALGAALIAKVAGFDKVLTSDGGGTSTDVSVVIDGEPTLTTE
GSVGAFPSKIPMIDVVTVGAGGGSIAWLSPEGTLKVGPQSAGADPGPLCYGKGGTEVTITDAHVVLGRIPPHLLGGEIPL
DVDAARAGVEQLAAKLGLTPEACATGILEISAWNQANALRQVTVKRGLDVRDFTLTTFGGSGSLLLCRLMDVLGIPTVLV
PPNPGNVSAFGLLTVDVKNDYVQTHVTLDDHLDPAAVTGIYDELTARAAEALAKEGFEPAEHQFVRTADLRYFGQAFEVR
VPVAAGALDPQAVADAFHAEHKALYGYDFSGDSSQQVEWVNLRVSGIGPIKRPAITRHPVESGVLATDSPDSTVTTPDST
VRAVCFDAAEGYVDTPVLWRPDLAPGTVLDGPAIIEEFGSTVPLHPGFTARIDEYLNIIVTRSQA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=757, Percent_Identity=29.4583883751651, Blast_Score=204, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI133901900, Length=733, Percent_Identity=24.2837653478854, Blast_Score=146, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322634, Length=749, Percent_Identity=28.8384512683578, Blast_Score=220, Evalue=7e-58,
Organism=Drosophila melanogaster, GI45550492, Length=744, Percent_Identity=28.7634408602151, Blast_Score=224, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 74113; Mature: 73982

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSIRIGIDTGGTFTDVVAFDEDSGELVTTKTPSTPSNPADGFLAGIDKVLAVLGASGA
CCCEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
DIDAVSHGTTVATNQLLEGKVDRLGFITTAGYEAMLEIARQSVPDGYGNSYFWVKPDRIV
CCCCCCCCCCHHHHHHHHCCHHHEEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCC
PRDRVKGVGGRLDFTGAEVRPFDEDAARAAARWFRAQGINTLGVCFLHAYANPEHEERMR
CHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHH
AVLAEEHPDAVVSLSSEVLREYREYERAMTTLVDAAVKPRLSAYVSNIKARLAGYVPAGA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGGAPRVVPFYVMKSNGGVLSADEVVHQPITTVLSGPAAGALGAALIAKVAGFDKVLTSD
CCCCCCEEEEEEEECCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEECC
GGGTSTDVSVVIDGEPTLTTEGSVGAFPSKIPMIDVVTVGAGGGSIAWLSPEGTLKVGPQ
CCCCCCEEEEEECCCCCEECCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEECCC
SAGADPGPLCYGKGGTEVTITDAHVVLGRIPPHLLGGEIPLDVDAARAGVEQLAAKLGLT
CCCCCCCCEEECCCCCEEEEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
PEACATGILEISAWNQANALRQVTVKRGLDVRDFTLTTFGGSGSLLLCRLMDVLGIPTVL
HHHHHCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCEEE
VPPNPGNVSAFGLLTVDVKNDYVQTHVTLDDHLDPAAVTGIYDELTARAAEALAKEGFEP
ECCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AEHQFVRTADLRYFGQAFEVRVPVAAGALDPQAVADAFHAEHKALYGYDFSGDSSQQVEW
HHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCEEE
VNLRVSGIGPIKRPAITRHPVESGVLATDSPDSTVTTPDSTVRAVCFDAAEGYVDTPVLW
EEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCHHEEEEEEECCCCCCCCCEEE
RPDLAPGTVLDGPAIIEEFGSTVPLHPGFTARIDEYLNIIVTRSQA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEECCC
>Mature Secondary Structure 
TRSIRIGIDTGGTFTDVVAFDEDSGELVTTKTPSTPSNPADGFLAGIDKVLAVLGASGA
CCEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
DIDAVSHGTTVATNQLLEGKVDRLGFITTAGYEAMLEIARQSVPDGYGNSYFWVKPDRIV
CCCCCCCCCCHHHHHHHHCCHHHEEEEEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCC
PRDRVKGVGGRLDFTGAEVRPFDEDAARAAARWFRAQGINTLGVCFLHAYANPEHEERMR
CHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHH
AVLAEEHPDAVVSLSSEVLREYREYERAMTTLVDAAVKPRLSAYVSNIKARLAGYVPAGA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SGGAPRVVPFYVMKSNGGVLSADEVVHQPITTVLSGPAAGALGAALIAKVAGFDKVLTSD
CCCCCCEEEEEEEECCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEECC
GGGTSTDVSVVIDGEPTLTTEGSVGAFPSKIPMIDVVTVGAGGGSIAWLSPEGTLKVGPQ
CCCCCCEEEEEECCCCCEECCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEECCC
SAGADPGPLCYGKGGTEVTITDAHVVLGRIPPHLLGGEIPLDVDAARAGVEQLAAKLGLT
CCCCCCCCEEECCCCCEEEEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
PEACATGILEISAWNQANALRQVTVKRGLDVRDFTLTTFGGSGSLLLCRLMDVLGIPTVL
HHHHHCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCEEE
VPPNPGNVSAFGLLTVDVKNDYVQTHVTLDDHLDPAAVTGIYDELTARAAEALAKEGFEP
ECCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
AEHQFVRTADLRYFGQAFEVRVPVAAGALDPQAVADAFHAEHKALYGYDFSGDSSQQVEW
HHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCEEE
VNLRVSGIGPIKRPAITRHPVESGVLATDSPDSTVTTPDSTVRAVCFDAAEGYVDTPVLW
EEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCHHEEEEEEECCCCCCCCCEEE
RPDLAPGTVLDGPAIIEEFGSTVPLHPGFTARIDEYLNIIVTRSQA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]