Click here to switch to the map view.

The map label for this gene is sigF [H]

Identifier: 119715629

GI number: 119715629

Start: 1470960

End: 1471742

Strand: Direct

Name: sigF [H]

Synonym: Noca_1393

Alternate gene names: 119715629

Gene position: 1470960-1471742 (Clockwise)

Preceding gene: 119715627

Following gene: 119715633

Centisome position: 29.5

GC content: 72.67

Gene sequence:

>783_bases
ATGGAGCACCGACGAGAGGCCACCGCCCGGCTCTTCGACGAGTTGGAGAACGCGTCCGACGACGAGCGCCGCCGTATTCG
CGACGAGATCGTCCGGCTCAACATGGGCGTCGCCAGCGACTGCGCCCGTCGCTACCGGCAGCGCGGCATCGCCACCGAGG
ACCTGGACCAGGTGGCCTACCTGGGGTTGGTCAAGGCGGTCCGCGGCTTCGAGCCGGGCCGCGGCCACGACTTCTTGTCC
TTCGCGGTGCCGACGATCCGTGGCGAGGTGCGCCGGCACTTCCGCGATCTCGGCTGGGCGCTGCGGCCGCCCCGATCGAT
CCAGGAGCTCCAGTCGCGCATTCACGCTGCCGAGGGCGACCTGTCCCAGGAGCTGGGTCGCTCGCCGCGGCCGAGCGACC
TCGCCCGCCACCTCGGGGTCGACCTGGAGCAGGTGCTCGACGCGCTGGGCGCGACCGGCTGCTTCGCCCCGGCCTCACTC
GACGTGGTCGGCGCCGACGGTGACGACTCCCTGGGCGACCGGCTCGGCACGGCGGATCCGGCGTTCGACCAGGCCGAGGC
ACGGATCACGCTGGCGGCGGTGCTGCGCGGACTGACGCCGAGGGAGCGTCGGATCCTCGAGCTGCGGTTCTTCGGCAACC
ACACCCAGGCCGAGATCGGCGCCGACATCGGCGTCACCCAGATGCAGGTCTCCCGGCTGCTGAACCGGATCCTGGCCCGC
CTGCGGGAGCGGATGCTCGCCGCCTCCGACGAGGCGCCGGTGTCCTCGGGCCCGGCGGCTTAG

Upstream 100 bases:

>100_bases
GCGGTCCCCCGTAAGGGTGGTGTGCCGTCTGGGGCGCGCTGCGAGGATCGAGTCGGCGGGCGCCTCGCGCGCCCGCCGCC
AATCGCCCAGGAGGTGCGAG

Downstream 100 bases:

>100_bases
GAGATCGGCGGCAGCTCCGAGGCGCGACCGGTGAAGGTGGGCGCCCGCTTCTCCAGGAACGCGGCGACGCCCTCCTTCCC
GTCACCGATCGAGGCCCAGT

Product: sigma-70 region 4 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEHRREATARLFDELENASDDERRRIRDEIVRLNMGVASDCARRYRQRGIATEDLDQVAYLGLVKAVRGFEPGRGHDFLS
FAVPTIRGEVRRHFRDLGWALRPPRSIQELQSRIHAAEGDLSQELGRSPRPSDLARHLGVDLEQVLDALGATGCFAPASL
DVVGADGDDSLGDRLGTADPAFDQAEARITLAAVLRGLTPRERRILELRFFGNHTQAEIGADIGVTQMQVSRLLNRILAR
LRERMLAASDEAPVSSGPAA

Sequences:

>Translated_260_residues
MEHRREATARLFDELENASDDERRRIRDEIVRLNMGVASDCARRYRQRGIATEDLDQVAYLGLVKAVRGFEPGRGHDFLS
FAVPTIRGEVRRHFRDLGWALRPPRSIQELQSRIHAAEGDLSQELGRSPRPSDLARHLGVDLEQVLDALGATGCFAPASL
DVVGADGDDSLGDRLGTADPAFDQAEARITLAAVLRGLTPRERRILELRFFGNHTQAEIGADIGVTQMQVSRLLNRILAR
LRERMLAASDEAPVSSGPAA
>Mature_260_residues
MEHRREATARLFDELENASDDERRRIRDEIVRLNMGVASDCARRYRQRGIATEDLDQVAYLGLVKAVRGFEPGRGHDFLS
FAVPTIRGEVRRHFRDLGWALRPPRSIQELQSRIHAAEGDLSQELGRSPRPSDLARHLGVDLEQVLDALGATGCFAPASL
DVVGADGDDSLGDRLGTADPAFDQAEARITLAAVLRGLTPRERRILELRFFGNHTQAEIGADIGVTQMQVSRLLNRILAR
LRERMLAASDEAPVSSGPAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=30.5676855895196, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=28.3185840707965, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=27.9411764705882, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28737; Mature: 28737

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHRREATARLFDELENASDDERRRIRDEIVRLNMGVASDCARRYRQRGIATEDLDQVAY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHH
LGLVKAVRGFEPGRGHDFLSFAVPTIRGEVRRHFRDLGWALRPPRSIQELQSRIHAAEGD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
LSQELGRSPRPSDLARHLGVDLEQVLDALGATGCFAPASLDVVGADGDDSLGDRLGTADP
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHCCCCCC
AFDQAEARITLAAVLRGLTPRERRILELRFFGNHTQAEIGADIGVTQMQVSRLLNRILAR
CCHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHH
LRERMLAASDEAPVSSGPAA
HHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEHRREATARLFDELENASDDERRRIRDEIVRLNMGVASDCARRYRQRGIATEDLDQVAY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHH
LGLVKAVRGFEPGRGHDFLSFAVPTIRGEVRRHFRDLGWALRPPRSIQELQSRIHAAEGD
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
LSQELGRSPRPSDLARHLGVDLEQVLDALGATGCFAPASLDVVGADGDDSLGDRLGTADP
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHCCCCCC
AFDQAEARITLAAVLRGLTPRERRILELRFFGNHTQAEIGADIGVTQMQVSRLLNRILAR
CCHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHH
LRERMLAASDEAPVSSGPAA
HHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]