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The map label for this gene is liaR [H]

Identifier: 119715554

GI number: 119715554

Start: 1390526

End: 1391203

Strand: Reverse

Name: liaR [H]

Synonym: Noca_1318

Alternate gene names: 119715554

Gene position: 1391203-1390526 (Counterclockwise)

Preceding gene: 119715555

Following gene: 119715553

Centisome position: 27.9

GC content: 71.98

Gene sequence:

>678_bases
ATGAACGCGGCCACGACCGTGCTGCTCGTCGACGACGATCCACTGGTGCGCTCCGCGCTGTCGCTGATGCTCGGTGGCCA
GAGTGACATCGAGGTCGTCGGGGAGGCCGCCGACGGCCACGAAGGGGTCCGGCTCGCCGCCGAGCTGCGACCGGACGTGG
TCCTGATGGACATCCGGATGCCCCGCCTCGACGGGCTCGGCGCGACCCGCGAGCTGCACGCCCGTCCCGCGCCGCCGCGG
GTGATCGTGCTGACGACCTTCGACGCCGACGAGCACGTCGTCGAGGCGCTCGCGGCGGGCGCCGACGGCTTCTTGCTCAA
GGACACCGCGCCGCCGCAGATCATCGAGGCGATCCGCAAGGTCGTGGACGACGAGCCGATGCTCTCCCCCTCGGTCACCC
GCACCCTGATCCGCCGGTTGCGCGACGAGCCCGATGCTGCCGCCGACGTCGCCGCGGCCCGCACGGCCGAGGCGCAGTCC
CGCCTCGCCCTGCTCACCGACAGGGAGCGCGACGTCGCGCTCGCGGTCGGGCGCGGCCTCAGCAACGCCGAGATCGCCGG
CGACCTCTATCTCTCCGTGCCCACGGTCAAAGCGCACGTCTCCCGGCTCTTCGACAAGCTCCAGGTGACCAACCGGGTCC
AGATCGCGATCTGCGTCCACGACGCGGGACTGAGCTAG

Upstream 100 bases:

>100_bases
TCGTGGGTCTCACGGAGCGGGCCGAGCTCCGGGGCGGCCGGCTCGAGGCCGGCCGGGACGGCTCGACCTTCGTCCTGCAC
GGCTGGATACCGTGGGCGGC

Downstream 100 bases:

>100_bases
CCGAGGCCAGAGGCCTGAGCTGGCAAAGACCGGAGCTCGTCGGCAGATCGAGGAGCGCACACCCGGCGGACACCGGTCCT
GAACGGCCTGTTCGGGCCAT

Product: response regulator receiver

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNAATTVLLVDDDPLVRSALSLMLGGQSDIEVVGEAADGHEGVRLAAELRPDVVLMDIRMPRLDGLGATRELHARPAPPR
VIVLTTFDADEHVVEALAAGADGFLLKDTAPPQIIEAIRKVVDDEPMLSPSVTRTLIRRLRDEPDAAADVAAARTAEAQS
RLALLTDRERDVALAVGRGLSNAEIAGDLYLSVPTVKAHVSRLFDKLQVTNRVQIAICVHDAGLS

Sequences:

>Translated_225_residues
MNAATTVLLVDDDPLVRSALSLMLGGQSDIEVVGEAADGHEGVRLAAELRPDVVLMDIRMPRLDGLGATRELHARPAPPR
VIVLTTFDADEHVVEALAAGADGFLLKDTAPPQIIEAIRKVVDDEPMLSPSVTRTLIRRLRDEPDAAADVAAARTAEAQS
RLALLTDRERDVALAVGRGLSNAEIAGDLYLSVPTVKAHVSRLFDKLQVTNRVQIAICVHDAGLS
>Mature_225_residues
MNAATTVLLVDDDPLVRSALSLMLGGQSDIEVVGEAADGHEGVRLAAELRPDVVLMDIRMPRLDGLGATRELHARPAPPR
VIVLTTFDADEHVVEALAAGADGFLLKDTAPPQIIEAIRKVVDDEPMLSPSVTRTLIRRLRDEPDAAADVAAARTAEAQS
RLALLTDRERDVALAVGRGLSNAEIAGDLYLSVPTVKAHVSRLFDKLQVTNRVQIAICVHDAGLS

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787473, Length=215, Percent_Identity=35.8139534883721, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788521, Length=213, Percent_Identity=36.6197183098592, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=32.3809523809524, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1790102, Length=211, Percent_Identity=33.6492890995261, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082052, Length=101, Percent_Identity=43.5643564356436, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=26.5700483091787, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=39.2523364485981, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24027; Mature: 24027

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAATTVLLVDDDPLVRSALSLMLGGQSDIEVVGEAADGHEGVRLAAELRPDVVLMDIRM
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEECC
PRLDGLGATRELHARPAPPRVIVLTTFDADEHVVEALAAGADGFLLKDTAPPQIIEAIRK
CCCCCCCCCHHHCCCCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
VVDDEPMLSPSVTRTLIRRLRDEPDAAADVAAARTAEAQSRLALLTDRERDVALAVGRGL
HHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCC
SNAEIAGDLYLSVPTVKAHVSRLFDKLQVTNRVQIAICVHDAGLS
CCCEECCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
>Mature Secondary Structure
MNAATTVLLVDDDPLVRSALSLMLGGQSDIEVVGEAADGHEGVRLAAELRPDVVLMDIRM
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEECC
PRLDGLGATRELHARPAPPRVIVLTTFDADEHVVEALAAGADGFLLKDTAPPQIIEAIRK
CCCCCCCCCHHHCCCCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
VVDDEPMLSPSVTRTLIRRLRDEPDAAADVAAARTAEAQSRLALLTDRERDVALAVGRGL
HHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCC
SNAEIAGDLYLSVPTVKAHVSRLFDKLQVTNRVQIAICVHDAGLS
CCCEECCEEEEECCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]