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The map label for this gene is yfkN [H]

Identifier: 119715535

GI number: 119715535

Start: 1372380

End: 1374227

Strand: Direct

Name: yfkN [H]

Synonym: Noca_1299

Alternate gene names: 119715535

Gene position: 1372380-1374227 (Clockwise)

Preceding gene: 119715534

Following gene: 119715539

Centisome position: 27.53

GC content: 68.29

Gene sequence:

>1848_bases
GTGTCCCAAGTCATGCCCTCACGCCGCGCCGTGCTCGCGGGCGGCGTCACCGGGGGAGCGGCCCTTGCCGCGTCCGGGTA
TGTCACGCCGTCGTACGCCTCGGAGGTGGACTCCCGCATCGGCAGAAGAGGGGTCCGGCTGACCGTGCTCGGCACCACCG
ACCTGCACGGCAACGTCTACAACTGGGACTACTTCAAGAACAAGGAGTTCGACAACTCCGCCCACGACGACATCGGCATC
GCCAAGGTCACGACCCTGGTCAAGGCGATGCGCGAGGAGCGCCGCGGCGAGCCGATCCTCACGCTGGACGCCGGCGACAC
CATCCAGGGCACGCCGCTGGCCTACTACTACGCCCGGATCGCGCCGATCACGGCCGGTGGCATCCACCCGATGGCCAACG
CCATGAACCTGGTCGGCTACGACGCCGCCGCCCTCGGCAACCACGAGTTCAACTACGGGCTGGACACGCTGCGGACCTTC
GAGGACCAGCTGAACTTCCCGCTGCTCGGGGCGAACGCCGTCGACCCCGCGACCGGGCTGCCGGCGTTCGCGCCGTACCT
GATCAAGACCTTCCGCGTCGGCCGCGGCCGCAAGCTCCGCGTCGGTGTGCTCGGTCTCACCAACCCCGGCATCGCGATCT
GGGACAAGGCCAACGTCGAGGGGAAGATGGAGTTCCCCGGCCTGGTCGAGCAGGCCAAGAAGTTCGTGCCGAAGATGAAG
GCCAAGGGCTGCGACGTCGTCATCGTCTCCGCGCACTCCGGCGCGGACACCTCCTCGTCGTACGGCGATGCGCTGCCGTT
CCCCGAGAACGCCGCGTCCCTCGTCGCCGAGCAGGTCCCGCACGTCGACGCGATCCTGGTCGGCCACGCGCACAAGGAGA
TCGCGCAGCGGTTCGTGCGCAACAAGGTCACCGGCCAGCAGGTGCTGCTGTGCGAGCCGTACTACTGGGGCATGCGGCTG
GCGGTCATGGATCTCGTCGTGCACGAGTCCTACACCAAGCAGGGCCGGCTCAAGTGGATCGTGACGGGAAGCGGCGCCCA
GCTGCTCAACTCGAACACCGTCGACGCGGACCCTGCGGTCGCGGCAGCGGTCCAGGAGCAGCACGACACCGTCGTGTCGT
ACGTGAACAGCCCGGTGGGCAGCTCGGCCGCAGCGATGTCGGCGGCACGCGCCGTGGTCGAGGACGTTCCGATCATCGAC
TTCGTGAACTACGTGCAGGCCGACGCGGTCAAGGCCGGTCTCAGTGGCGCCGCCGCGGCGCTCCCGGTGCTCTCCATCGC
GGCGCCGTTCAACCGCAGCGCGTCCTTCCCGTCCGGTGAGGTGACCGTGCGTGACGTTGCGGGCCTCTACATCTACGACA
ACACGCTGCTGGCGGTCCAGGTCAGCGGTGCGGACCTCAAGGCCTACCTCGAGTACAGCGCCCGCTACTTCAAGAAGATC
TCCGGCACCGGGCCGTTCACGATGGACCAGGTCACCAACGCGGTCACCAGCACGGCCCCGAACGGCACGCCGGACTACAA
CTACGACGCCGTCGCGGGGCTGGACGCACCGCTGACCTACGACATCGACATCTCCCAGGAGGTCGGGTCGCGGATCATCG
GGCTGTCCTACGACGGCAAGCTGGTCGGCGACGCCGACCAGTTCGTGCTGGCCGTCAACAACTACCGCCAGTCCGGTGGC
GGCGGGTTCCCGGCGGTCTCCACCGCCCCGGTCGTCTACAACCGGCAGAACGAGATCCGCCAGCTGCTCATCGACTGGGT
CACCGCCCACGGAACCATCGACCCGGCCCAGTTCGCCTCCGTCGACTGGCGCCTGGTCTCCGGTGGCCAGCCGATCACGG
TGAGCTGA

Upstream 100 bases:

>100_bases
GAAACCTGACGGTCATCGAGGCGTTGGCCGACAAATGGGCTCGCTGATGCCCCGGGAGTGATAGGACATTTCTTGTTCCT
ATCGATTCCAGGAGGAAGTT

Downstream 100 bases:

>100_bases
GCGTCGCCGACCGTCGCGCCCTCCTTCGCCGCGAGGCGGGGGAGGGCGCGGCTACTCGCCGCGGAACACCGGCCGGCGAT
GCTCGGAGAAGGCGCGCATC

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSQVMPSRRAVLAGGVTGGAALAASGYVTPSYASEVDSRIGRRGVRLTVLGTTDLHGNVYNWDYFKNKEFDNSAHDDIGI
AKVTTLVKAMREERRGEPILTLDAGDTIQGTPLAYYYARIAPITAGGIHPMANAMNLVGYDAAALGNHEFNYGLDTLRTF
EDQLNFPLLGANAVDPATGLPAFAPYLIKTFRVGRGRKLRVGVLGLTNPGIAIWDKANVEGKMEFPGLVEQAKKFVPKMK
AKGCDVVIVSAHSGADTSSSYGDALPFPENAASLVAEQVPHVDAILVGHAHKEIAQRFVRNKVTGQQVLLCEPYYWGMRL
AVMDLVVHESYTKQGRLKWIVTGSGAQLLNSNTVDADPAVAAAVQEQHDTVVSYVNSPVGSSAAAMSAARAVVEDVPIID
FVNYVQADAVKAGLSGAAAALPVLSIAAPFNRSASFPSGEVTVRDVAGLYIYDNTLLAVQVSGADLKAYLEYSARYFKKI
SGTGPFTMDQVTNAVTSTAPNGTPDYNYDAVAGLDAPLTYDIDISQEVGSRIIGLSYDGKLVGDADQFVLAVNNYRQSGG
GGFPAVSTAPVVYNRQNEIRQLLIDWVTAHGTIDPAQFASVDWRLVSGGQPITVS

Sequences:

>Translated_615_residues
MSQVMPSRRAVLAGGVTGGAALAASGYVTPSYASEVDSRIGRRGVRLTVLGTTDLHGNVYNWDYFKNKEFDNSAHDDIGI
AKVTTLVKAMREERRGEPILTLDAGDTIQGTPLAYYYARIAPITAGGIHPMANAMNLVGYDAAALGNHEFNYGLDTLRTF
EDQLNFPLLGANAVDPATGLPAFAPYLIKTFRVGRGRKLRVGVLGLTNPGIAIWDKANVEGKMEFPGLVEQAKKFVPKMK
AKGCDVVIVSAHSGADTSSSYGDALPFPENAASLVAEQVPHVDAILVGHAHKEIAQRFVRNKVTGQQVLLCEPYYWGMRL
AVMDLVVHESYTKQGRLKWIVTGSGAQLLNSNTVDADPAVAAAVQEQHDTVVSYVNSPVGSSAAAMSAARAVVEDVPIID
FVNYVQADAVKAGLSGAAAALPVLSIAAPFNRSASFPSGEVTVRDVAGLYIYDNTLLAVQVSGADLKAYLEYSARYFKKI
SGTGPFTMDQVTNAVTSTAPNGTPDYNYDAVAGLDAPLTYDIDISQEVGSRIIGLSYDGKLVGDADQFVLAVNNYRQSGG
GGFPAVSTAPVVYNRQNEIRQLLIDWVTAHGTIDPAQFASVDWRLVSGGQPITVS
>Mature_614_residues
SQVMPSRRAVLAGGVTGGAALAASGYVTPSYASEVDSRIGRRGVRLTVLGTTDLHGNVYNWDYFKNKEFDNSAHDDIGIA
KVTTLVKAMREERRGEPILTLDAGDTIQGTPLAYYYARIAPITAGGIHPMANAMNLVGYDAAALGNHEFNYGLDTLRTFE
DQLNFPLLGANAVDPATGLPAFAPYLIKTFRVGRGRKLRVGVLGLTNPGIAIWDKANVEGKMEFPGLVEQAKKFVPKMKA
KGCDVVIVSAHSGADTSSSYGDALPFPENAASLVAEQVPHVDAILVGHAHKEIAQRFVRNKVTGQQVLLCEPYYWGMRLA
VMDLVVHESYTKQGRLKWIVTGSGAQLLNSNTVDADPAVAAAVQEQHDTVVSYVNSPVGSSAAAMSAARAVVEDVPIIDF
VNYVQADAVKAGLSGAAAALPVLSIAAPFNRSASFPSGEVTVRDVAGLYIYDNTLLAVQVSGADLKAYLEYSARYFKKIS
GTGPFTMDQVTNAVTSTAPNGTPDYNYDAVAGLDAPLTYDIDISQEVGSRIIGLSYDGKLVGDADQFVLAVNNYRQSGGG
GFPAVSTAPVVYNRQNEIRQLLIDWVTAHGTIDPAQFASVDWRLVSGGQPITVS

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=601, Percent_Identity=21.7970049916805, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1790658, Length=624, Percent_Identity=33.1730769230769, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1786687, Length=224, Percent_Identity=29.9107142857143, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28573524, Length=468, Percent_Identity=22.4358974358974, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922446, Length=447, Percent_Identity=22.3713646532438, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24654424, Length=447, Percent_Identity=22.3713646532438, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922444, Length=230, Percent_Identity=28.2608695652174, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 65487; Mature: 65355

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVMPSRRAVLAGGVTGGAALAASGYVTPSYASEVDSRIGRRGVRLTVLGTTDLHGNVY
CCCCCCCCCEEEECCCCCCHHHEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEE
NWDYFKNKEFDNSAHDDIGIAKVTTLVKAMREERRGEPILTLDAGDTIQGTPLAYYYARI
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEE
APITAGGIHPMANAMNLVGYDAAALGNHEFNYGLDTLRTFEDQLNFPLLGANAVDPATGL
CEEECCCCCHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCC
PAFAPYLIKTFRVGRGRKLRVGVLGLTNPGIAIWDKANVEGKMEFPGLVEQAKKFVPKMK
CHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AKGCDVVIVSAHSGADTSSSYGDALPFPENAASLVAEQVPHVDAILVGHAHKEIAQRFVR
CCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
NKVTGQQVLLCEPYYWGMRLAVMDLVVHESYTKQGRLKWIVTGSGAQLLNSNTVDADPAV
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCHHH
AAAVQEQHDTVVSYVNSPVGSSAAAMSAARAVVEDVPIIDFVNYVQADAVKAGLSGAAAA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
LPVLSIAAPFNRSASFPSGEVTVRDVAGLYIYDNTLLAVQVSGADLKAYLEYSARYFKKI
HHHHHHCCCCCCCCCCCCCCEEEEEECEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHH
SGTGPFTMDQVTNAVTSTAPNGTPDYNYDAVAGLDAPLTYDIDISQEVGSRIIGLSYDGK
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEECCHHHCCEEEEEECCCE
LVGDADQFVLAVNNYRQSGGGGFPAVSTAPVVYNRQNEIRQLLIDWVTAHGTIDPAQFAS
EECCCCEEEEEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHCC
VDWRLVSGGQPITVS
CCEEEECCCCEEEEC
>Mature Secondary Structure 
SQVMPSRRAVLAGGVTGGAALAASGYVTPSYASEVDSRIGRRGVRLTVLGTTDLHGNVY
CCCCCCCCEEEECCCCCCHHHEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEE
NWDYFKNKEFDNSAHDDIGIAKVTTLVKAMREERRGEPILTLDAGDTIQGTPLAYYYARI
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEE
APITAGGIHPMANAMNLVGYDAAALGNHEFNYGLDTLRTFEDQLNFPLLGANAVDPATGL
CEEECCCCCHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCC
PAFAPYLIKTFRVGRGRKLRVGVLGLTNPGIAIWDKANVEGKMEFPGLVEQAKKFVPKMK
CHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AKGCDVVIVSAHSGADTSSSYGDALPFPENAASLVAEQVPHVDAILVGHAHKEIAQRFVR
CCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
NKVTGQQVLLCEPYYWGMRLAVMDLVVHESYTKQGRLKWIVTGSGAQLLNSNTVDADPAV
HCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCHHH
AAAVQEQHDTVVSYVNSPVGSSAAAMSAARAVVEDVPIIDFVNYVQADAVKAGLSGAAAA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
LPVLSIAAPFNRSASFPSGEVTVRDVAGLYIYDNTLLAVQVSGADLKAYLEYSARYFKKI
HHHHHHCCCCCCCCCCCCCCEEEEEECEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHH
SGTGPFTMDQVTNAVTSTAPNGTPDYNYDAVAGLDAPLTYDIDISQEVGSRIIGLSYDGK
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEECCHHHCCEEEEEECCCE
LVGDADQFVLAVNNYRQSGGGGFPAVSTAPVVYNRQNEIRQLLIDWVTAHGTIDPAQFAS
EECCCCEEEEEEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHCC
VDWRLVSGGQPITVS
CCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]