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The map label for this gene is amiB2 [H]

Identifier: 119715464

GI number: 119715464

Start: 1305288

End: 1306688

Strand: Direct

Name: amiB2 [H]

Synonym: Noca_1228

Alternate gene names: 119715464

Gene position: 1305288-1306688 (Clockwise)

Preceding gene: 119715463

Following gene: 119715465

Centisome position: 26.18

GC content: 75.73

Gene sequence:

>1401_bases
ATGACCGATCTCTCCCGAGCCGGGGTGGCCGAGACCGCACGACTGGTCGCGGGGGGTGAGGCCTCGGCGCGCGGCGTGGT
CGAGCACGCCCTGGCCCGTATGGAGGAGCGCGACCGCGGGCTGAACGCCATCTCCCAGCTCCTGGTCGACGCGGCGATCG
CGGAGGCGCAGGCGCGCGACGAGGCGCTGGTCGCCGGCGGTCCGATCGGCCCGCTGCACGGCGTACCGGTCGTGATCAAG
GAGGAGATCGACGTCGCCGGCACGGTGACCACGTTCGGCGGCGAGGCCAACTCGACGCCGGCCGACGCGGACGCCGAGGT
GGTACGCCGACTCCGCGCGGCCGGTGCGGTGATCGTCGCCAAGACGACCATGCCGGAGTTCGGGGCGTTCCCGTTCACCG
AGTCCGCCTCACGCGGTGTGACCCGCAACCCCTGGGACCCGAGCCGCACCCCGGGCGGCTCCAGCGGAGGGACCGCCGTG
GCGGTCTCGGCCGGAATGGTGCCGGCCGGCATGGGTGGGGACGGCGGCGGCTCGATCCGGATCCCGAGCGCCAGCTGCGG
GCTGTTCGGGCTCAAGCCCCAGCGCGGCCGGGTGACCACCGCCCCGCACCCGCACCTGTGGTGGGCGCTGGGCACCGCCG
GGCCGCTGACCCGCAGCGTGCTCGACAGCGCGCTGGTCTACGACGCGATCCGCGGCAACGTCGCCGGCGACCTCTACACC
GCCGGCGGCGACGAGCCGTTCGTGGCGGCCGCCGGCCGCGAGCCGGGGCGGCTGCGGATCGGCTGGTCGGTCAAGCCGGT
GACCCTGGGCGTGCGCCCCGACCCGGTGCACGTCCGCGCGGTCGAGGACACCGCCCGGCTGCTGACCGACCTGGGCCACG
ACGTCCGGGAGGTCGACCCGCGCTACCCGGACCCGACGGCCGCGTTCGTGCCGCAGTTCTTCGCCGGCATCCGCACCGAG
GCCGACCAGGTCGAGCACTTCGAGCGGCTCGAGCGCCGCACCCGCGAGACCTACCGGCTCGGGTCCTGGGTGACGCCCAA
GGTCATCGACCGCGCGCTCCGCACGACCGAGCAGGTCGCCGCCAAGGCGAACCGGGTCTTCGAGGACGTCGACGTGCTGC
TCACCCCGGCGATCGCGCACCGGCCGCCCCGCGTCGGCCGCCTCGACGGCGTCGGCACCGTGCGCGCCTCGCTGCGGGCG
ATGCCCTCGATCGCCTACGCCGCGCTCTGGAACGTGGCCGGCAACCCGGCTGCGTCGGTGCCCTGCGGCCTCGCTGCGGA
CGGCCTCCCGGTCGCCGTGCAGCTGGTCGGCCGTACCGACGACGAGGCGACCTTGTTCTCCCTCTCCGCGCAGCTCGAGG
CCGCCCGGCCGTGGCCGCTGCTCGCGGGCGACCCCGCCTGA

Upstream 100 bases:

>100_bases
GGACCTGGATGGGGCGAGGTCCCGCCGTTGGTGACCAAACCACAAGGTTTCCGGACCACCGAGCCGCGCGATCGGCCCCG
CCAGCCCATAGCCTGGGCGC

Downstream 100 bases:

>100_bases
TGCCGGCGATCGCGGTCGCCGACCTCACCATGACGTACGGCGAGCTGCGGGCGGTCGACGGCGTCAGGCTCCAGGTCGGC
GAGGGCGAGTTCTTCGGCAT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTDLSRAGVAETARLVAGGEASARGVVEHALARMEERDRGLNAISQLLVDAAIAEAQARDEALVAGGPIGPLHGVPVVIK
EEIDVAGTVTTFGGEANSTPADADAEVVRRLRAAGAVIVAKTTMPEFGAFPFTESASRGVTRNPWDPSRTPGGSSGGTAV
AVSAGMVPAGMGGDGGGSIRIPSASCGLFGLKPQRGRVTTAPHPHLWWALGTAGPLTRSVLDSALVYDAIRGNVAGDLYT
AGGDEPFVAAAGREPGRLRIGWSVKPVTLGVRPDPVHVRAVEDTARLLTDLGHDVREVDPRYPDPTAAFVPQFFAGIRTE
ADQVEHFERLERRTRETYRLGSWVTPKVIDRALRTTEQVAAKANRVFEDVDVLLTPAIAHRPPRVGRLDGVGTVRASLRA
MPSIAYAALWNVAGNPAASVPCGLAADGLPVAVQLVGRTDDEATLFSLSAQLEAARPWPLLAGDPA

Sequences:

>Translated_466_residues
MTDLSRAGVAETARLVAGGEASARGVVEHALARMEERDRGLNAISQLLVDAAIAEAQARDEALVAGGPIGPLHGVPVVIK
EEIDVAGTVTTFGGEANSTPADADAEVVRRLRAAGAVIVAKTTMPEFGAFPFTESASRGVTRNPWDPSRTPGGSSGGTAV
AVSAGMVPAGMGGDGGGSIRIPSASCGLFGLKPQRGRVTTAPHPHLWWALGTAGPLTRSVLDSALVYDAIRGNVAGDLYT
AGGDEPFVAAAGREPGRLRIGWSVKPVTLGVRPDPVHVRAVEDTARLLTDLGHDVREVDPRYPDPTAAFVPQFFAGIRTE
ADQVEHFERLERRTRETYRLGSWVTPKVIDRALRTTEQVAAKANRVFEDVDVLLTPAIAHRPPRVGRLDGVGTVRASLRA
MPSIAYAALWNVAGNPAASVPCGLAADGLPVAVQLVGRTDDEATLFSLSAQLEAARPWPLLAGDPA
>Mature_465_residues
TDLSRAGVAETARLVAGGEASARGVVEHALARMEERDRGLNAISQLLVDAAIAEAQARDEALVAGGPIGPLHGVPVVIKE
EIDVAGTVTTFGGEANSTPADADAEVVRRLRAAGAVIVAKTTMPEFGAFPFTESASRGVTRNPWDPSRTPGGSSGGTAVA
VSAGMVPAGMGGDGGGSIRIPSASCGLFGLKPQRGRVTTAPHPHLWWALGTAGPLTRSVLDSALVYDAIRGNVAGDLYTA
GGDEPFVAAAGREPGRLRIGWSVKPVTLGVRPDPVHVRAVEDTARLLTDLGHDVREVDPRYPDPTAAFVPQFFAGIRTEA
DQVEHFERLERRTRETYRLGSWVTPKVIDRALRTTEQVAAKANRVFEDVDVLLTPAIAHRPPRVGRLDGVGTVRASLRAM
PSIAYAALWNVAGNPAASVPCGLAADGLPVAVQLVGRTDDEATLFSLSAQLEAARPWPLLAGDPA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=486, Percent_Identity=24.2798353909465, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI222831590, Length=274, Percent_Identity=25.1824817518248, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI166795287, Length=184, Percent_Identity=35.8695652173913, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=26.7068273092369, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=283, Percent_Identity=31.8021201413428, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=466, Percent_Identity=27.6824034334764, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=325, Percent_Identity=27.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=278, Percent_Identity=32.0143884892086, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=278, Percent_Identity=32.0143884892086, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=254, Percent_Identity=31.496062992126, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI32565337, Length=501, Percent_Identity=23.3532934131737, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=152, Percent_Identity=36.1842105263158, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=161, Percent_Identity=38.5093167701863, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=126, Percent_Identity=39.6825396825397, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356731, Length=500, Percent_Identity=27.4, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI161078093, Length=494, Percent_Identity=25.9109311740891, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=30.2231237322515, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45550774, Length=236, Percent_Identity=32.2033898305085, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648435, Length=236, Percent_Identity=32.2033898305085, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648437, Length=236, Percent_Identity=32.2033898305085, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24644968, Length=246, Percent_Identity=30.8943089430894, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=35.8823529411765, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=35.8823529411765, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652985, Length=224, Percent_Identity=33.0357142857143, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922090, Length=224, Percent_Identity=33.0357142857143, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652981, Length=224, Percent_Identity=33.0357142857143, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652983, Length=224, Percent_Identity=33.0357142857143, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 48718; Mature: 48586

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSRAGVAETARLVAGGEASARGVVEHALARMEERDRGLNAISQLLVDAAIAEAQARD
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
EALVAGGPIGPLHGVPVVIKEEIDVAGTVTTFGGEANSTPADADAEVVRRLRAAGAVIVA
CEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEE
KTTMPEFGAFPFTESASRGVTRNPWDPSRTPGGSSGGTAVAVSAGMVPAGMGGDGGGSIR
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
IPSASCGLFGLKPQRGRVTTAPHPHLWWALGTAGPLTRSVLDSALVYDAIRGNVAGDLYT
ECCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
AGGDEPFVAAAGREPGRLRIGWSVKPVTLGVRPDPVHVRAVEDTARLLTDLGHDVREVDP
CCCCCCEEEECCCCCCEEEEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHCCHHHHCCC
RYPDPTAAFVPQFFAGIRTEADQVEHFERLERRTRETYRLGSWVTPKVIDRALRTTEQVA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AKANRVFEDVDVLLTPAIAHRPPRVGRLDGVGTVRASLRAMPSIAYAALWNVAGNPAASV
HHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PCGLAADGLPVAVQLVGRTDDEATLFSLSAQLEAARPWPLLAGDPA
CCCCCCCCCCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TDLSRAGVAETARLVAGGEASARGVVEHALARMEERDRGLNAISQLLVDAAIAEAQARD
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
EALVAGGPIGPLHGVPVVIKEEIDVAGTVTTFGGEANSTPADADAEVVRRLRAAGAVIVA
CEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEEE
KTTMPEFGAFPFTESASRGVTRNPWDPSRTPGGSSGGTAVAVSAGMVPAGMGGDGGGSIR
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
IPSASCGLFGLKPQRGRVTTAPHPHLWWALGTAGPLTRSVLDSALVYDAIRGNVAGDLYT
ECCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
AGGDEPFVAAAGREPGRLRIGWSVKPVTLGVRPDPVHVRAVEDTARLLTDLGHDVREVDP
CCCCCCEEEECCCCCCEEEEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHCCHHHHCCC
RYPDPTAAFVPQFFAGIRTEADQVEHFERLERRTRETYRLGSWVTPKVIDRALRTTEQVA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AKANRVFEDVDVLLTPAIAHRPPRVGRLDGVGTVRASLRAMPSIAYAALWNVAGNPAASV
HHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PCGLAADGLPVAVQLVGRTDDEATLFSLSAQLEAARPWPLLAGDPA
CCCCCCCCCCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]