| Definition | Nocardioides sp. JS614 chromosome, complete genome. |
|---|---|
| Accession | NC_008699 |
| Length | 4,985,871 |
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The map label for this gene is 119715454
Identifier: 119715454
GI number: 119715454
Start: 1290899
End: 1292350
Strand: Direct
Name: 119715454
Synonym: Noca_1218
Alternate gene names: NA
Gene position: 1290899-1292350 (Clockwise)
Preceding gene: 119715451
Following gene: 119715455
Centisome position: 25.89
GC content: 73.69
Gene sequence:
>1452_bases ATGACCGAGAGGCTCCGTCCGCGTGACGTGGCCATCCTCGCCGAGGAGTCCGCGTCGACGCCGATGCACAACGCGACCGT CGAGATCTTCGAGCCCGGCCAGTCCGGCTTCGACTACGCGCGGCTGGTCCGGCTGGTGCAGGAGCGGATCTCGTTCGTCC CGCGCTACCGCCAGCGGGTCCAGCCGGTGCCGGGCCGCCTCGCCAACCCGGTGTGGGTCGACGACGAGCACTTCGACATC GGCTACCACGTACGCCGCTCCGCGCTGCCCCGGCCGGGCACCCTCGACCAGCTCCGCGACCTGGTGGCGCGGATCGTGTC GCGCCCGCTGGACCGCAGCCGCCCGCTGTGGGAGATGTACTTCGTCGAGGGGCTGGCCGACGGCCAGGTCGCGATCCTCA CCAAGTCGCACCAGGTGCTGGTCGACGGCATCGAGACCGTCGACCTGGGCCAGGTGCTGCTCGACAAGTCGCCGGAGCCC AAGGAGCTGGGCGCCGACGGCTGGCGGGCCCGCGCCCGCCCCTCGCCCATCGCGCTGATGGCCGGCGCGGTCCACGACTC GCTGACCGGCCCGGAGACCGTGGCCGAGACGCTGCTCAGCACGACCGGGTCGGTGCTGCGCGGGGCCGCCTCGAGCGCCC GGTCCGCGGGCCGCGTCTTCGACGCGCTCGCGAACCGCAGCGTCGCCGGCGACTCGCCGATCATCGGGCCGCTGTCGCAG CAGCGCCGGATCGTCGCGGTGCGCACCGACCTCGCCGACTACCGTAAGGTCCGCGAGGCGCACGGCGGCACCGTGAACGA CGTCGTGCTCGCGACCGTCACCGGCGCGCTGCGAGCCTGGCTGATGACCCGCGGGGAGTCGATGCGCGGGATCCGGCAGA TCAGAGCCGTCGTCCCGGTCTCGGTGATCGACGAGGAGCTCGAGGCGACCTCGCTCGGGAGCCAGATCGCCGCCCACTTC GTCGACCTGCCGGTGTGGGAGGCCAGCCCGGTGGTGCGCCTCCACCAGGTCTCGTACTCCTTCCAGGCGCACAAGGAGAC CGGTCGCGCGGTCGGGGCCAGCCGGCTGGCCAGCATGTCCGGCTTCGCGCCGGCGACCTTCCACGCGATCGGCTCCCGCG TCGCCACGGTCGAGCGCCGCCGCGGGTACCAGCTCAGCGTCACCAACGTCCCCGGTCCGCAGGCGCCGCTGTACGCCGCC GGGGCCCGGATGGTCTCGACCTACCCGGTCCCGCCGCTGCTCGAGGGCCACCCGCTGGCGATCGGGGTGACGTCGTACGA CGGGCACGTGTACTACGGCCTGACCGCCGATCGCGACCTGCTGCCCGACGCCGACCTCCTCGGCCCGTGCGTCCAGGAGG CGCTCGACGAGCTCGTGGACTCCGCGTCCGGCAGCCGCAGCCGCGCCCCCCGTGGACGCAAGAAGCGGCAGCCCCCCGAG GAGCCGTCGTGA
Upstream 100 bases:
>100_bases CCTGCTGCTGGGGCTGCGTCGACCTCGGTAACGTTCGTCAGGATCGCACGTCGGACCAACGCCCGATGCGCCGCCCCGGC GGTGCGACGAAAGGGACACC
Downstream 100 bases:
>100_bases CGGTCCGGGCCTACCTGCCGACCACGATCGCCCTGCTCGCCGAGCAGCACGCCCAGGGCGGGTTCGCGGTCACCGACGAG GTCGTCGTGCTCGACGGCGC
Product: diacylglycerol O-acyltransferase
Products: NA
Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468 [H]
Number of amino acids: Translated: 483; Mature: 482
Protein sequence:
>483_residues MTERLRPRDVAILAEESASTPMHNATVEIFEPGQSGFDYARLVRLVQERISFVPRYRQRVQPVPGRLANPVWVDDEHFDI GYHVRRSALPRPGTLDQLRDLVARIVSRPLDRSRPLWEMYFVEGLADGQVAILTKSHQVLVDGIETVDLGQVLLDKSPEP KELGADGWRARARPSPIALMAGAVHDSLTGPETVAETLLSTTGSVLRGAASSARSAGRVFDALANRSVAGDSPIIGPLSQ QRRIVAVRTDLADYRKVREAHGGTVNDVVLATVTGALRAWLMTRGESMRGIRQIRAVVPVSVIDEELEATSLGSQIAAHF VDLPVWEASPVVRLHQVSYSFQAHKETGRAVGASRLASMSGFAPATFHAIGSRVATVERRRGYQLSVTNVPGPQAPLYAA GARMVSTYPVPPLLEGHPLAIGVTSYDGHVYYGLTADRDLLPDADLLGPCVQEALDELVDSASGSRSRAPRGRKKRQPPE EPS
Sequences:
>Translated_483_residues MTERLRPRDVAILAEESASTPMHNATVEIFEPGQSGFDYARLVRLVQERISFVPRYRQRVQPVPGRLANPVWVDDEHFDI GYHVRRSALPRPGTLDQLRDLVARIVSRPLDRSRPLWEMYFVEGLADGQVAILTKSHQVLVDGIETVDLGQVLLDKSPEP KELGADGWRARARPSPIALMAGAVHDSLTGPETVAETLLSTTGSVLRGAASSARSAGRVFDALANRSVAGDSPIIGPLSQ QRRIVAVRTDLADYRKVREAHGGTVNDVVLATVTGALRAWLMTRGESMRGIRQIRAVVPVSVIDEELEATSLGSQIAAHF VDLPVWEASPVVRLHQVSYSFQAHKETGRAVGASRLASMSGFAPATFHAIGSRVATVERRRGYQLSVTNVPGPQAPLYAA GARMVSTYPVPPLLEGHPLAIGVTSYDGHVYYGLTADRDLLPDADLLGPCVQEALDELVDSASGSRSRAPRGRKKRQPPE EPS >Mature_482_residues TERLRPRDVAILAEESASTPMHNATVEIFEPGQSGFDYARLVRLVQERISFVPRYRQRVQPVPGRLANPVWVDDEHFDIG YHVRRSALPRPGTLDQLRDLVARIVSRPLDRSRPLWEMYFVEGLADGQVAILTKSHQVLVDGIETVDLGQVLLDKSPEPK ELGADGWRARARPSPIALMAGAVHDSLTGPETVAETLLSTTGSVLRGAASSARSAGRVFDALANRSVAGDSPIIGPLSQQ RRIVAVRTDLADYRKVREAHGGTVNDVVLATVTGALRAWLMTRGESMRGIRQIRAVVPVSVIDEELEATSLGSQIAAHFV DLPVWEASPVVRLHQVSYSFQAHKETGRAVGASRLASMSGFAPATFHAIGSRVATVERRRGYQLSVTNVPGPQAPLYAAG ARMVSTYPVPPLLEGHPLAIGVTSYDGHVYYGLTADRDLLPDADLLGPCVQEALDELVDSASGSRSRAPRGRKKRQPPEE PS
Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the long-chain O-acyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014292 - InterPro: IPR004255 [H]
Pfam domain/function: PF03007 UPF0089 [H]
EC number: =2.3.1.20 [H]
Molecular weight: Translated: 52469; Mature: 52338
Theoretical pI: Translated: 8.43; Mature: 8.43
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTERLRPRDVAILAEESASTPMHNATVEIFEPGQSGFDYARLVRLVQERISFVPRYRQRV CCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHC QPVPGRLANPVWVDDEHFDIGYHVRRSALPRPGTLDQLRDLVARIVSRPLDRSRPLWEMY CCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH FVEGLADGQVAILTKSHQVLVDGIETVDLGQVLLDKSPEPKELGADGWRARARPSPIALM HHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCEEE AGAVHDSLTGPETVAETLLSTTGSVLRGAASSARSAGRVFDALANRSVAGDSPIIGPLSQ EHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC QRRIVAVRTDLADYRKVREAHGGTVNDVVLATVTGALRAWLMTRGESMRGIRQIRAVVPV CCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH SVIDEELEATSLGSQIAAHFVDLPVWEASPVVRLHQVSYSFQAHKETGRAVGASRLASMS HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHC GFAPATFHAIGSRVATVERRRGYQLSVTNVPGPQAPLYAAGARMVSTYPVPPLLEGHPLA CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCHHEECCCCCCCCCCCCEE IGVTSYDGHVYYGLTADRDLLPDADLLGPCVQEALDELVDSASGSRSRAPRGRKKRQPPE EEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC EPS CCC >Mature Secondary Structure TERLRPRDVAILAEESASTPMHNATVEIFEPGQSGFDYARLVRLVQERISFVPRYRQRV CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHC QPVPGRLANPVWVDDEHFDIGYHVRRSALPRPGTLDQLRDLVARIVSRPLDRSRPLWEMY CCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH FVEGLADGQVAILTKSHQVLVDGIETVDLGQVLLDKSPEPKELGADGWRARARPSPIALM HHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCEEE AGAVHDSLTGPETVAETLLSTTGSVLRGAASSARSAGRVFDALANRSVAGDSPIIGPLSQ EHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC QRRIVAVRTDLADYRKVREAHGGTVNDVVLATVTGALRAWLMTRGESMRGIRQIRAVVPV CCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH SVIDEELEATSLGSQIAAHFVDLPVWEASPVVRLHQVSYSFQAHKETGRAVGASRLASMS HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHC GFAPATFHAIGSRVATVERRRGYQLSVTNVPGPQAPLYAAGARMVSTYPVPPLLEGHPLA CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCHHEECCCCCCCCCCCCEE IGVTSYDGHVYYGLTADRDLLPDADLLGPCVQEALDELVDSASGSRSRAPRGRKKRQPPE EEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC EPS CCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9634230; 12218036 [H]