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The map label for this gene is aspS [H]

Identifier: 119715425

GI number: 119715425

Start: 1262969

End: 1264243

Strand: Reverse

Name: aspS [H]

Synonym: Noca_1189

Alternate gene names: 119715425

Gene position: 1264243-1262969 (Counterclockwise)

Preceding gene: 119715427

Following gene: 119715414

Centisome position: 25.36

GC content: 74.2

Gene sequence:

>1275_bases
ATGCACCGCACGCTCTGTTCCGACCTGCCCGCCGCGACGCCCGGCTCGACCGTGCGGCTCCAAGGCTGGGTGCACCGGCG
TCGTGAGCTGTCCGCGCTGACCTTCCTGGTGGTCCGCGACCGCAGCGGCCTGGCCCAGGTCGTGGTCCGCGACGGCTCGG
CCGTGCCGCCCGAGGAGACGACCGTCGAGGTGGTCGGCACCGCGACCGCGAACGCCCAGGCGCCGGGCGGGATGGAGCTG
ACCGACCCGGTCGTCACCGCGCTGACCGAGCCGGCCGCGACCCCACCGGTGGAGCTGTGGCGCCCCGGCCTGGACGTCGT
GCTGCCGACCCTGCTCGACCACGCGCCGGTGACCTGGCGACACCCGCGGCAGCGGGCCCGGTGGGAGCTGGCCGCGGCGT
CGGTCCGGGGCTTCCGCGCGACCCTCGACGCCGCCGGCTTCACCGAGGTCCAGACCCCGAAGTTCGTGGCGTCGGCGACC
GAGTCGGGGGCGAACGTGTTCGAGGTCGACTACTTCGGGCGGCCGGCGTACCTGGCCCAGAGCCCGCAGTTCTACAAGCA
GCAGCTGGTCGGCGTCTTCGAGCGGGTCTACGAGGTCGGCCCGGTGTTCCGCGCCGAGCCGCACGACACGGTGCGGCACC
TGGCGGAGTACGTCTCGCTCGACGTCGAGCTCGGCTTCGTCCGCGACCACCGCGACGTGCTGTCGGTGCTGCGCGACGTG
CTCGCCGGGATGGTCGCGGCGGTGCACGAGCACGTCGGCGCCGCGGTGGAACGGTCCGGCGCGGCGATACCGGTCGTTCC
CGAGGAGATCCCGGTCCTGCACTTCCGCGACGCGCTGGCACTGGTCGGTGCCCCCGAGGACGAGCCCGACCTGGCGCCCG
AGCACGAGCGGGCGATCGGCGCCTGGGCGCTCACCGAGCACGGCAGCGACTTCGTGGCCGTCGAGGGCTACCCGATGGCG
AAGCGACCGTTCTACACGCACCCGTGGCCGGGCGAGGAGAGGTGGTCGAACAGCTTCGACCTGCTGTTCCGCGGGCTCGA
GCTGGTCACCGGGGGGCAGCGCCTGCACCGGCCCTCGGAGTACGACGCCGCGATCCGCGCCCGGGGCGAGGACCCGGCGG
CGTACGCGCCGTACCTCCAGGCCTTCGAGCACGGCATGCCGCCGCACGGCGGGTTCGCGATTGGCCTGGAGCGGTGGACG
GCCCGGCTCACCGGGGTCGCGAACATCCGCGAGGTGACGCTGTTCCCGCGCGACCTGACCCGGCTGACCCCCTGA

Upstream 100 bases:

>100_bases
GAGGCCGGGACCCGGCGACGGGCAATCGGCGAAGGTGTGGTGGCACCGCGACCTTGCCCCCGCCCACACCTCCAGGGCTT
CACCGCAACTGGAGGTAGTC

Downstream 100 bases:

>100_bases
GGGCCGGCGCGGGGAGTCGGTTTCCCCGGTGAACCGGGGAAACCGGAACTGTTGGGCCGAAACTTCGGGCCAGAGGTTCC
AGCTTCGGCCCAACAGTCCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MHRTLCSDLPAATPGSTVRLQGWVHRRRELSALTFLVVRDRSGLAQVVVRDGSAVPPEETTVEVVGTATANAQAPGGMEL
TDPVVTALTEPAATPPVELWRPGLDVVLPTLLDHAPVTWRHPRQRARWELAAASVRGFRATLDAAGFTEVQTPKFVASAT
ESGANVFEVDYFGRPAYLAQSPQFYKQQLVGVFERVYEVGPVFRAEPHDTVRHLAEYVSLDVELGFVRDHRDVLSVLRDV
LAGMVAAVHEHVGAAVERSGAAIPVVPEEIPVLHFRDALALVGAPEDEPDLAPEHERAIGAWALTEHGSDFVAVEGYPMA
KRPFYTHPWPGEERWSNSFDLLFRGLELVTGGQRLHRPSEYDAAIRARGEDPAAYAPYLQAFEHGMPPHGGFAIGLERWT
ARLTGVANIREVTLFPRDLTRLTP

Sequences:

>Translated_424_residues
MHRTLCSDLPAATPGSTVRLQGWVHRRRELSALTFLVVRDRSGLAQVVVRDGSAVPPEETTVEVVGTATANAQAPGGMEL
TDPVVTALTEPAATPPVELWRPGLDVVLPTLLDHAPVTWRHPRQRARWELAAASVRGFRATLDAAGFTEVQTPKFVASAT
ESGANVFEVDYFGRPAYLAQSPQFYKQQLVGVFERVYEVGPVFRAEPHDTVRHLAEYVSLDVELGFVRDHRDVLSVLRDV
LAGMVAAVHEHVGAAVERSGAAIPVVPEEIPVLHFRDALALVGAPEDEPDLAPEHERAIGAWALTEHGSDFVAVEGYPMA
KRPFYTHPWPGEERWSNSFDLLFRGLELVTGGQRLHRPSEYDAAIRARGEDPAAYAPYLQAFEHGMPPHGGFAIGLERWT
ARLTGVANIREVTLFPRDLTRLTP
>Mature_424_residues
MHRTLCSDLPAATPGSTVRLQGWVHRRRELSALTFLVVRDRSGLAQVVVRDGSAVPPEETTVEVVGTATANAQAPGGMEL
TDPVVTALTEPAATPPVELWRPGLDVVLPTLLDHAPVTWRHPRQRARWELAAASVRGFRATLDAAGFTEVQTPKFVASAT
ESGANVFEVDYFGRPAYLAQSPQFYKQQLVGVFERVYEVGPVFRAEPHDTVRHLAEYVSLDVELGFVRDHRDVLSVLRDV
LAGMVAAVHEHVGAAVERSGAAIPVVPEEIPVLHFRDALALVGAPEDEPDLAPEHERAIGAWALTEHGSDFVAVEGYPMA
KRPFYTHPWPGEERWSNSFDLLFRGLELVTGGQRLHRPSEYDAAIRARGEDPAAYAPYLQAFEHGMPPHGGFAIGLERWT
ARLTGVANIREVTLFPRDLTRLTP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=454, Percent_Identity=33.4801762114537, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI4758762, Length=435, Percent_Identity=28.5057471264368, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI226491502, Length=454, Percent_Identity=25.5506607929515, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1787161, Length=460, Percent_Identity=26.9565217391304, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=28.4584980237154, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=328, Percent_Identity=38.719512195122, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71984122, Length=440, Percent_Identity=30, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17556372, Length=315, Percent_Identity=25.7142857142857, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=352, Percent_Identity=37.5, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6321807, Length=433, Percent_Identity=28.6374133949192, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17136276, Length=454, Percent_Identity=32.5991189427313, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI19921528, Length=444, Percent_Identity=27.027027027027, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI19922416, Length=455, Percent_Identity=23.956043956044, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 46558; Mature: 46558

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRTLCSDLPAATPGSTVRLQGWVHRRRELSALTFLVVRDRSGLAQVVVRDGSAVPPEET
CCCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCCCCCC
TVEVVGTATANAQAPGGMELTDPVVTALTEPAATPPVELWRPGLDVVLPTLLDHAPVTWR
EEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCC
HPRQRARWELAAASVRGFRATLDAAGFTEVQTPKFVASATESGANVFEVDYFGRPAYLAQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHCC
SPQFYKQQLVGVFERVYEVGPVFRAEPHDTVRHLAEYVSLDVELGFVRDHRDVLSVLRDV
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHH
LAGMVAAVHEHVGAAVERSGAAIPVVPEEIPVLHFRDALALVGAPEDEPDLAPEHERAIG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHEECCCCCCCCCCCCHHHHHE
AWALTEHGSDFVAVEGYPMAKRPFYTHPWPGEERWSNSFDLLFRGLELVTGGQRLHRPSE
EEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHCCCCH
YDAAIRARGEDPAAYAPYLQAFEHGMPPHGGFAIGLERWTARLTGVANIREVTLFPRDLT
HCHHHEECCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCEEEEEECCHHHH
RLTP
HCCC
>Mature Secondary Structure
MHRTLCSDLPAATPGSTVRLQGWVHRRRELSALTFLVVRDRSGLAQVVVRDGSAVPPEET
CCCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCCCCCC
TVEVVGTATANAQAPGGMELTDPVVTALTEPAATPPVELWRPGLDVVLPTLLDHAPVTWR
EEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCC
HPRQRARWELAAASVRGFRATLDAAGFTEVQTPKFVASATESGANVFEVDYFGRPAYLAQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHCC
SPQFYKQQLVGVFERVYEVGPVFRAEPHDTVRHLAEYVSLDVELGFVRDHRDVLSVLRDV
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHH
LAGMVAAVHEHVGAAVERSGAAIPVVPEEIPVLHFRDALALVGAPEDEPDLAPEHERAIG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHEECCCCCCCCCCCCHHHHHE
AWALTEHGSDFVAVEGYPMAKRPFYTHPWPGEERWSNSFDLLFRGLELVTGGQRLHRPSE
EEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHCCCCH
YDAAIRARGEDPAAYAPYLQAFEHGMPPHGGFAIGLERWTARLTGVANIREVTLFPRDLT
HCHHHEECCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCEEEEEECCHHHH
RLTP
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]