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The map label for this gene is ykfB [H]

Identifier: 119715080

GI number: 119715080

Start: 876960

End: 878063

Strand: Direct

Name: ykfB [H]

Synonym: Noca_0835

Alternate gene names: 119715080

Gene position: 876960-878063 (Clockwise)

Preceding gene: 119715079

Following gene: 119715081

Centisome position: 17.59

GC content: 69.02

Gene sequence:

>1104_bases
ATGAAGATCACCAGGGTCGAGGCGGTCCCCTACGCGATCCCGTACGTCAAGCCGCTGCGCTTCGCCAGCGGCGAGGTGCA
CGTCGCCGAACACGTCCTGGTGCGGGTCCACACCGACGACGGGATCGTCGGGGTCGCGGAGGCGCCCCCGCGGCCCTTCA
CCTACGGTGAGACGCAGGCCGGCATCATCGCGGTGATCGAGAAGATCTTCGTCCCCCAGCTCCTCGGGCTGACGCTGCTC
GACCGCGAGGTCATCCAAGAACGCCTCCAGCGCACGGTCGGGAACCCCGCAGCCAAGTCGGCCATCGACATCGCCGTCTG
GGATGCACTCGGACGCACGCTCAAGCAGCCGGTCAGCGCCCTCCTCGGCGGCTACACCACCGGTCTGCGGGTGTGTCACA
TGCTGGGCTTCGAGGAGCCGGCGAAGATGGTCGCCGAAGCGGAGCGGATGGTCGAGACCTACGGCATCCGGACGTTCAAG
GTGAAGGTCGGCCGTCGGCCGGTGGCCCTCGACACCGCGGTGGTCCGGGCACTGCGGGAGCGCTTCGGCGATGCGGTCGA
GCTCTATGTCGACGGCAACCGGGGCTGGAGCCCCACGGAGTCGCTGCAGGCGATGAAGGAGATGGCCGACCTGGGCCTGA
CCTGCGCGGAGGAGCTGTGTCCCGCCGACGACGTGCTGGGCAGACGCTGGCTGGTCTCCCACCTCGACGTGCCGTTCATC
GCCGACGAATCGGCCACGTCCGCGGCCGAGGCGACGCGGGAGGTGCTCGCGGGTGCCGCGACGGCCCTGAGCATCAAGTG
CGCCAGGACCGGCTTCACGCAGAGTCGGCGCGTCCACCACCTCGCCGAGGGCCTGGGCCTCGAGGTCGTCATGGGCAACC
AGATCGACGGGCAGCTGGGGACCGCGTGCACCGTCGCCTTCGGTGCTGCCTACCGGCTCACCTCGCGTCGCGCCGCCGAG
GTCTCGAACTTCCTGGACATGAGCGACGACCTCCTTGTCGAGCCGCTCCAGATCCGCGACGGTGAGATCGCCGTGGGCCA
GGGGATCGGGCTATCGGTGGAGATCGACGAAGACAAGCTCTCCCATTACCGCATCGACCGCTAG

Upstream 100 bases:

>100_bases
GCGACCGGCCGCGCCCGGTGGTGACGATGGTGGTCTCCGTCCTCGCCGCCGACGACGCCCTGGCGTCGCTGTCGAGCAGC
TTCGTCGACGGGATCCTCGC

Downstream 100 bases:

>100_bases
GCAACGCGCGATCGATGCGGTGGCTCCGGGAAAGGCTCCCGGGAGTCCGTACCTCGCGAAGTGCAGAAGGAAGGAAATGA
AGATGAGCTCGATCGAAGTC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKITRVEAVPYAIPYVKPLRFASGEVHVAEHVLVRVHTDDGIVGVAEAPPRPFTYGETQAGIIAVIEKIFVPQLLGLTLL
DREVIQERLQRTVGNPAAKSAIDIAVWDALGRTLKQPVSALLGGYTTGLRVCHMLGFEEPAKMVAEAERMVETYGIRTFK
VKVGRRPVALDTAVVRALRERFGDAVELYVDGNRGWSPTESLQAMKEMADLGLTCAEELCPADDVLGRRWLVSHLDVPFI
ADESATSAAEATREVLAGAATALSIKCARTGFTQSRRVHHLAEGLGLEVVMGNQIDGQLGTACTVAFGAAYRLTSRRAAE
VSNFLDMSDDLLVEPLQIRDGEIAVGQGIGLSVEIDEDKLSHYRIDR

Sequences:

>Translated_367_residues
MKITRVEAVPYAIPYVKPLRFASGEVHVAEHVLVRVHTDDGIVGVAEAPPRPFTYGETQAGIIAVIEKIFVPQLLGLTLL
DREVIQERLQRTVGNPAAKSAIDIAVWDALGRTLKQPVSALLGGYTTGLRVCHMLGFEEPAKMVAEAERMVETYGIRTFK
VKVGRRPVALDTAVVRALRERFGDAVELYVDGNRGWSPTESLQAMKEMADLGLTCAEELCPADDVLGRRWLVSHLDVPFI
ADESATSAAEATREVLAGAATALSIKCARTGFTQSRRVHHLAEGLGLEVVMGNQIDGQLGTACTVAFGAAYRLTSRRAAE
VSNFLDMSDDLLVEPLQIRDGEIAVGQGIGLSVEIDEDKLSHYRIDR
>Mature_367_residues
MKITRVEAVPYAIPYVKPLRFASGEVHVAEHVLVRVHTDDGIVGVAEAPPRPFTYGETQAGIIAVIEKIFVPQLLGLTLL
DREVIQERLQRTVGNPAAKSAIDIAVWDALGRTLKQPVSALLGGYTTGLRVCHMLGFEEPAKMVAEAERMVETYGIRTFK
VKVGRRPVALDTAVVRALRERFGDAVELYVDGNRGWSPTESLQAMKEMADLGLTCAEELCPADDVLGRRWLVSHLDVPFI
ADESATSAAEATREVLAGAATALSIKCARTGFTQSRRVHHLAEGLGLEVVMGNQIDGQLGTACTVAFGAAYRLTSRRAAE
VSNFLDMSDDLLVEPLQIRDGEIAVGQGIGLSVEIDEDKLSHYRIDR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=260, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39899; Mature: 39899

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRVEAVPYAIPYVKPLRFASGEVHVAEHVLVRVHTDDGIVGVAEAPPRPFTYGETQA
CCCEEEECCCCCCCCCCHHHHCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCH
GIIAVIEKIFVPQLLGLTLLDREVIQERLQRTVGNPAAKSAIDIAVWDALGRTLKQPVSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGYTTGLRVCHMLGFEEPAKMVAEAERMVETYGIRTFKVKVGRRPVALDTAVVRALRE
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHH
RFGDAVELYVDGNRGWSPTESLQAMKEMADLGLTCAEELCPADDVLGRRWLVSHLDVPFI
HCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCEE
ADESATSAAEATREVLAGAATALSIKCARTGFTQSRRVHHLAEGLGLEVVMGNQIDGQLG
ECCCCHHHHHHHHHHHHHHHHHEEEEHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCC
TACTVAFGAAYRLTSRRAAEVSNFLDMSDDLLVEPLQIRDGEIAVGQGIGLSVEIDEDKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEECCCCEEECCCCEEEEEECHHHH
SHYRIDR
CCCCCCC
>Mature Secondary Structure
MKITRVEAVPYAIPYVKPLRFASGEVHVAEHVLVRVHTDDGIVGVAEAPPRPFTYGETQA
CCCEEEECCCCCCCCCCHHHHCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCH
GIIAVIEKIFVPQLLGLTLLDREVIQERLQRTVGNPAAKSAIDIAVWDALGRTLKQPVSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLGGYTTGLRVCHMLGFEEPAKMVAEAERMVETYGIRTFKVKVGRRPVALDTAVVRALRE
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHH
RFGDAVELYVDGNRGWSPTESLQAMKEMADLGLTCAEELCPADDVLGRRWLVSHLDVPFI
HCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCEE
ADESATSAAEATREVLAGAATALSIKCARTGFTQSRRVHHLAEGLGLEVVMGNQIDGQLG
ECCCCHHHHHHHHHHHHHHHHHEEEEHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCC
TACTVAFGAAYRLTSRRAAEVSNFLDMSDDLLVEPLQIRDGEIAVGQGIGLSVEIDEDKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEECCCCEEECCCCEEEEEECHHHH
SHYRIDR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]