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The map label for this gene is hemD [H]

Identifier: 119714760

GI number: 119714760

Start: 526010

End: 527668

Strand: Direct

Name: hemD [H]

Synonym: Noca_0496

Alternate gene names: 119714760

Gene position: 526010-527668 (Clockwise)

Preceding gene: 119714759

Following gene: 119714761

Centisome position: 10.55

GC content: 74.14

Gene sequence:

>1659_bases
ATGACGCGAGGCAAGACCACCACCGCCACCGGCTCGGGATCCACTCGAGGCTGGGTGTCGTTCGTCGGCAGCGGCCCGGG
CGACCCCGGCCTGCTGACCGTGCGCGCCGTCGACCTGCTCCGGCAGGCGGACGTCGTGGTGACCGAGGTGCCCGACCACG
CGGCGCTGGTCCGCACGGTGCTCGGCCTGCCGCTCGACGCCGCGGGCGCCGAGCCGGCCGGCCCGGAGATCGTCGACGGC
GGCTTCGGCGAGGACGGCCAGCCACTGACCCACGCGGCGCGCGCGAAGGTCGTGGTCCGCCAGGCCAAGCGCGGCCTGAG
GGTGGTCCGGCTGATGGCCGGCGACCCGTTCCTCTACGCCTCCGGGCCGGAGGAGGCGCAGGCCTGCGTCAAGGCGGGCC
TCGGCTTCGAGATCGTCCCCGGGGTGTCCTCGGTCAGCGCGGTCCCGGCGTACGCGGGCATCCCGCTCACCACCAAGGAC
TGTCGCGAGGTCGCTGTCGTCACCTGCGGCGAGCGGGTCGACTGGAGCCGGTACGTCGACGCGCGCACCCTCGTGCTGCT
CTCGGCCGTCGGCCAGATCGCGGACATCAGCAAGGCCCTGGTCGCGGCCGGCCGCTCGCCGGAGACGCCGGTCGCGATGA
CCCGGGTCGGCACCACCACCGAGCAGGCGACGGTCACCTCCACGCTGGAGCGGATCGGTGCCGACGCGCGCGCCGCCCGG
ATGACGCCGCCGGCGATCACCGTCGTGGGCGACGTCGTCGACCTGCGCGAGACGCTGTCGTGGTTCGAGACCAAGCCGCT
GTTCGGCTGGCGGGTGCTGGTGCCCCGCACCAAGGAGCAGGCCGGCTCGCTGTCCGCGCGCCTGCGCGGCTACGGCGCCG
TGCCCGATGAGGTCCCGACCATCTCGGTCGAGCCGCCGCGCAACCCGCTGCAGATGGACAAGGCCGTCCGCGGCCTGGTC
GAGGGCCGGTACGAGTGGATCGCGTTCACCTCGGTGAACGCGGTGCGCGCCGTCCGGGAGAAGTTCGAGGAGTACGGCCT
GGACGCGCGGGCCTTCTCGGGCCTGAAGATCGCGGCGGTCGGCGACAAGACCGCCCAGGCGATCGCCGGCTGGGGCCTGC
GGGCCGACCTGGTGCCGTCCGGCGAGCAGTCCGCGGCCGGGCTGCTGGCCGACTGGCCGGAGTACGACGAGCTCCTCGAC
CCGATCAACCGGGTGTTCCTGCCGCGCGCCGACATCGCCACCGAGAACCTCGTGGCCGGGCTCGTGGACCTGGGCTGGGA
GTGCGACGACGTGACGGCGTACCGGACCGTCCGCGCGGCCCCGCCGCCGGCGCCGACCCGGGACGCGATCAAGACCGGCA
AGTTCGATGCGGTCGTCTTCACGTCCTCGTCCACGGTGCGCAACCTGGTCGGCATCGCCGGCAAGCCGCACCCGTCGACG
ATCATCGCCGTGATCGGGCCGGCCACCGCGAAGACCGCGGAGGAGCACGGCCTGCGGGTCGACGTGCTGGCGGCCAAGCC
GGACGTGGACACGCTGGTGGACGCGCTCGCCGACTTCGGCGCCTCGCGCCGGATCGCGATGGTCGAGGCCGGCCAGCCGG
TGACCAAGCCGTCGGACCGCAAGCCGTCCGGCCGCCGGAAGACGACCTCGGCGCGATGA

Upstream 100 bases:

>100_bases
CAGCAATGAACGGACCCCGAGGCCGTGGACCGCTCGATCCAACCCATGACCAACCGCTCGATGAACGCTCGATGATCACA
GCAGACAAGGTGCAGAACGC

Downstream 100 bases:

>100_bases
GCCCGGTCCGATGAGCGAGGTCCGATGAGCGAGGTCCCGGTCCCGCCGGTCGTCGGGCCGACCGTCCGGCCGCGACGGCT
GCGCACCACCGCGGCCCTGC

Product: uroporphyrinogen-III synthase / uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTRGKTTTATGSGSTRGWVSFVGSGPGDPGLLTVRAVDLLRQADVVVTEVPDHAALVRTVLGLPLDAAGAEPAGPEIVDG
GFGEDGQPLTHAARAKVVVRQAKRGLRVVRLMAGDPFLYASGPEEAQACVKAGLGFEIVPGVSSVSAVPAYAGIPLTTKD
CREVAVVTCGERVDWSRYVDARTLVLLSAVGQIADISKALVAAGRSPETPVAMTRVGTTTEQATVTSTLERIGADARAAR
MTPPAITVVGDVVDLRETLSWFETKPLFGWRVLVPRTKEQAGSLSARLRGYGAVPDEVPTISVEPPRNPLQMDKAVRGLV
EGRYEWIAFTSVNAVRAVREKFEEYGLDARAFSGLKIAAVGDKTAQAIAGWGLRADLVPSGEQSAAGLLADWPEYDELLD
PINRVFLPRADIATENLVAGLVDLGWECDDVTAYRTVRAAPPPAPTRDAIKTGKFDAVVFTSSSTVRNLVGIAGKPHPST
IIAVIGPATAKTAEEHGLRVDVLAAKPDVDTLVDALADFGASRRIAMVEAGQPVTKPSDRKPSGRRKTTSAR

Sequences:

>Translated_552_residues
MTRGKTTTATGSGSTRGWVSFVGSGPGDPGLLTVRAVDLLRQADVVVTEVPDHAALVRTVLGLPLDAAGAEPAGPEIVDG
GFGEDGQPLTHAARAKVVVRQAKRGLRVVRLMAGDPFLYASGPEEAQACVKAGLGFEIVPGVSSVSAVPAYAGIPLTTKD
CREVAVVTCGERVDWSRYVDARTLVLLSAVGQIADISKALVAAGRSPETPVAMTRVGTTTEQATVTSTLERIGADARAAR
MTPPAITVVGDVVDLRETLSWFETKPLFGWRVLVPRTKEQAGSLSARLRGYGAVPDEVPTISVEPPRNPLQMDKAVRGLV
EGRYEWIAFTSVNAVRAVREKFEEYGLDARAFSGLKIAAVGDKTAQAIAGWGLRADLVPSGEQSAAGLLADWPEYDELLD
PINRVFLPRADIATENLVAGLVDLGWECDDVTAYRTVRAAPPPAPTRDAIKTGKFDAVVFTSSSTVRNLVGIAGKPHPST
IIAVIGPATAKTAEEHGLRVDVLAAKPDVDTLVDALADFGASRRIAMVEAGQPVTKPSDRKPSGRRKTTSAR
>Mature_551_residues
TRGKTTTATGSGSTRGWVSFVGSGPGDPGLLTVRAVDLLRQADVVVTEVPDHAALVRTVLGLPLDAAGAEPAGPEIVDGG
FGEDGQPLTHAARAKVVVRQAKRGLRVVRLMAGDPFLYASGPEEAQACVKAGLGFEIVPGVSSVSAVPAYAGIPLTTKDC
REVAVVTCGERVDWSRYVDARTLVLLSAVGQIADISKALVAAGRSPETPVAMTRVGTTTEQATVTSTLERIGADARAARM
TPPAITVVGDVVDLRETLSWFETKPLFGWRVLVPRTKEQAGSLSARLRGYGAVPDEVPTISVEPPRNPLQMDKAVRGLVE
GRYEWIAFTSVNAVRAVREKFEEYGLDARAFSGLKIAAVGDKTAQAIAGWGLRADLVPSGEQSAAGLLADWPEYDELLDP
INRVFLPRADIATENLVAGLVDLGWECDDVTAYRTVRAAPPPAPTRDAIKTGKFDAVVFTSSSTVRNLVGIAGKPHPSTI
IAVIGPATAKTAEEHGLRVDVLAAKPDVDTLVDALADFGASRRIAMVEAGQPVTKPSDRKPSGRRKTTSAR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=259, Percent_Identity=35.1351351351351, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6322922, Length=243, Percent_Identity=25.5144032921811, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 58171; Mature: 58040

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGKTTTATGSGSTRGWVSFVGSGPGDPGLLTVRAVDLLRQADVVVTEVPDHAALVRTV
CCCCCCEECCCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCEEEEECCCHHHHHHHH
LGLPLDAAGAEPAGPEIVDGGFGEDGQPLTHAARAKVVVRQAKRGLRVVRLMAGDPFLYA
HCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEC
SGPEEAQACVKAGLGFEIVPGVSSVSAVPAYAGIPLTTKDCREVAVVTCGERVDWSRYVD
CCHHHHHHHHHHCCCEEEECCCCHHHCCCHHCCCCCCHHHHHHEEEEECCCCCCHHHHHH
ARTLVLLSAVGQIADISKALVAAGRSPETPVAMTRVGTTTEQATVTSTLERIGADARAAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHC
MTPPAITVVGDVVDLRETLSWFETKPLFGWRVLVPRTKEQAGSLSARLRGYGAVPDEVPT
CCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCHHHHHCCCCCCCCCCCE
ISVEPPRNPLQMDKAVRGLVEGRYEWIAFTSVNAVRAVREKFEEYGLDARAFSGLKIAAV
EEECCCCCHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHCCEEEEEE
GDKTAQAIAGWGLRADLVPSGEQSAAGLLADWPEYDELLDPINRVFLPRADIATENLVAG
CCHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
LVDLGWECDDVTAYRTVRAAPPPAPTRDAIKTGKFDAVVFTSSSTVRNLVGIAGKPHPST
HHHCCCCCCCCHHHHEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCE
IIAVIGPATAKTAEEHGLRVDVLAAKPDVDTLVDALADFGASRRIAMVEAGQPVTKPSDR
EEEEECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
KPSGRRKTTSAR
CCCCCCCCCCCC
>Mature Secondary Structure 
TRGKTTTATGSGSTRGWVSFVGSGPGDPGLLTVRAVDLLRQADVVVTEVPDHAALVRTV
CCCCCEECCCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCEEEEECCCHHHHHHHH
LGLPLDAAGAEPAGPEIVDGGFGEDGQPLTHAARAKVVVRQAKRGLRVVRLMAGDPFLYA
HCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEC
SGPEEAQACVKAGLGFEIVPGVSSVSAVPAYAGIPLTTKDCREVAVVTCGERVDWSRYVD
CCHHHHHHHHHHCCCEEEECCCCHHHCCCHHCCCCCCHHHHHHEEEEECCCCCCHHHHHH
ARTLVLLSAVGQIADISKALVAAGRSPETPVAMTRVGTTTEQATVTSTLERIGADARAAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHC
MTPPAITVVGDVVDLRETLSWFETKPLFGWRVLVPRTKEQAGSLSARLRGYGAVPDEVPT
CCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHCCHHHHHCCCCCCCCCCCE
ISVEPPRNPLQMDKAVRGLVEGRYEWIAFTSVNAVRAVREKFEEYGLDARAFSGLKIAAV
EEECCCCCHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHCCEEEEEE
GDKTAQAIAGWGLRADLVPSGEQSAAGLLADWPEYDELLDPINRVFLPRADIATENLVAG
CCHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
LVDLGWECDDVTAYRTVRAAPPPAPTRDAIKTGKFDAVVFTSSSTVRNLVGIAGKPHPST
HHHCCCCCCCCHHHHEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCE
IIAVIGPATAKTAEEHGLRVDVLAAKPDVDTLVDALADFGASRRIAMVEAGQPVTKPSDR
EEEEECCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
KPSGRRKTTSAR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]