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The map label for this gene is galE [C]

Identifier: 119714750

GI number: 119714750

Start: 515440

End: 516519

Strand: Direct

Name: galE [C]

Synonym: Noca_0486

Alternate gene names: 119714750

Gene position: 515440-516519 (Clockwise)

Preceding gene: 119714749

Following gene: 119714751

Centisome position: 10.34

GC content: 73.52

Gene sequence:

>1080_bases
ATGGGCAGCGCCGGCAGGGTCGTCCTGGTCACCGGGGTCTCACGCGACCTCGGCCGCCGGTTCGCGCGCGCCCTGGCGGC
CGACCCGTCCGTCGACCGCGTGGTCGGGGTGGACGCCGTACCGCCGCGCGGTGACATCGGCGAGGTCTCCTTCGTCCGGG
CCGACATCCGCAACCCGGTCATCGCGAAGGTCATCGTCAAGGAGGACGTCGACACCGTCGTCCACATGAGCGTCATCTCC
ACCCCCGGCAGCGCCGGGGGGCGGAACACGATGAAGGAGCTCAACGTCATCGGGACGATGCAGCTCCTCGCCGCCTGCCA
GAAGGCTCCCGGCCTGCGCACGCTCGTGGTGAAGTCCTCGACCACGGTCTACGGCGCCAGCAACCGCGACCCGGCGATGT
TCACCGAGGACATGGAGCCGCGCCGGGTGCCGCGCTCCGGCTACGCCAAGGACGTCGCCGAGGTCGAGGGCTACGTGCGC
GGTTTCGCCCGCCGCCGTCCCGACGTCTCGGTGACCATCCTCCGCGCGGCCAACGTGATCGGGCCCCAGGTCACGAGCCC
GCTCACCTCCTACTTCCGGTTGCCGGTGATCCCCACCGTGCTCGGCTTCGACCCGCGGCTGCAGTTCCTGCACGAGCAGG
ACCTGCTCGACGTGCTGCGGCATGCGATCGCCGCGGAGGTCGCCGGGACCTTCAACGTCGCCGGTGCCGGGATCCTGATG
CTCTCCCAGGCGGTACGCCGCCTCCAGCGGCCCAGCGTCCCGCTGCCGCCCTTCGCGGTCGGCCGGGTCGGCTCCACCCT
GCGCTCGGCGCGGGTCGCGGACTTCTCGCCCGAGCAGCTCGGCCTGCTCACGTACGGGCGTGGCGTCGACACGACCCGGA
TGCGTACCGAGCTCGGCTTCGAGCCGGCCCTCACGACCGCTGGGGCGTTCGCGGACTTCGCGGCCCCGCTGCCGCCCACC
GGCGGCTACGCCGACCGGCTGCTGGCCCGGGTCGCGCAGGGCCTGCCCGAGCCCGCACCCGTCCCCGCGCCGCTACCGGC
CTCGGCGCCGGCGTACCCCCGAGGAGCCGACCGTGGGTGA

Upstream 100 bases:

>100_bases
AGCGCATGGCCAAGAAGAAGCACCGCAAGCTGCTGAAGAAGACGCGCGTGCAGCGTCGCAAGCTCGGCAAGTAGCGCCCG
AGCCGATCACAGCACGCCCC

Downstream 100 bases:

>100_bases
CGCGGAGATCATCCCGATCGGGACCCGCGGCCGGCCGGGCCGCGGCACGGGCAAGCACCCGTCCTCGGCCGCCCGCGGCC
TCGCGACCGGCGCTCGACCG

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGSAGRVVLVTGVSRDLGRRFARALAADPSVDRVVGVDAVPPRGDIGEVSFVRADIRNPVIAKVIVKEDVDTVVHMSVIS
TPGSAGGRNTMKELNVIGTMQLLAACQKAPGLRTLVVKSSTTVYGASNRDPAMFTEDMEPRRVPRSGYAKDVAEVEGYVR
GFARRRPDVSVTILRAANVIGPQVTSPLTSYFRLPVIPTVLGFDPRLQFLHEQDLLDVLRHAIAAEVAGTFNVAGAGILM
LSQAVRRLQRPSVPLPPFAVGRVGSTLRSARVADFSPEQLGLLTYGRGVDTTRMRTELGFEPALTTAGAFADFAAPLPPT
GGYADRLLARVAQGLPEPAPVPAPLPASAPAYPRGADRG

Sequences:

>Translated_359_residues
MGSAGRVVLVTGVSRDLGRRFARALAADPSVDRVVGVDAVPPRGDIGEVSFVRADIRNPVIAKVIVKEDVDTVVHMSVIS
TPGSAGGRNTMKELNVIGTMQLLAACQKAPGLRTLVVKSSTTVYGASNRDPAMFTEDMEPRRVPRSGYAKDVAEVEGYVR
GFARRRPDVSVTILRAANVIGPQVTSPLTSYFRLPVIPTVLGFDPRLQFLHEQDLLDVLRHAIAAEVAGTFNVAGAGILM
LSQAVRRLQRPSVPLPPFAVGRVGSTLRSARVADFSPEQLGLLTYGRGVDTTRMRTELGFEPALTTAGAFADFAAPLPPT
GGYADRLLARVAQGLPEPAPVPAPLPASAPAYPRGADRG
>Mature_358_residues
GSAGRVVLVTGVSRDLGRRFARALAADPSVDRVVGVDAVPPRGDIGEVSFVRADIRNPVIAKVIVKEDVDTVVHMSVIST
PGSAGGRNTMKELNVIGTMQLLAACQKAPGLRTLVVKSSTTVYGASNRDPAMFTEDMEPRRVPRSGYAKDVAEVEGYVRG
FARRRPDVSVTILRAANVIGPQVTSPLTSYFRLPVIPTVLGFDPRLQFLHEQDLLDVLRHAIAAEVAGTFNVAGAGILML
SQAVRRLQRPSVPLPPFAVGRVGSTLRSARVADFSPEQLGLLTYGRGVDTTRMRTELGFEPALTTAGAFADFAAPLPPTG
GYADRLLARVAQGLPEPAPVPAPLPASAPAYPRGADRG

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 38060; Mature: 37929

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSAGRVVLVTGVSRDLGRRFARALAADPSVDRVVGVDAVPPRGDIGEVSFVRADIRNPV
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCCC
IAKVIVKEDVDTVVHMSVISTPGSAGGRNTMKELNVIGTMQLLAACQKAPGLRTLVVKSS
EEHHHHHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
TTVYGASNRDPAMFTEDMEPRRVPRSGYAKDVAEVEGYVRGFARRRPDVSVTILRAANVI
CEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHC
GPQVTSPLTSYFRLPVIPTVLGFDPRLQFLHEQDLLDVLRHAIAAEVAGTFNVAGAGILM
CCHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LSQAVRRLQRPSVPLPPFAVGRVGSTLRSARVADFSPEQLGLLTYGRGVDTTRMRTELGF
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHCCC
EPALTTAGAFADFAAPLPPTGGYADRLLARVAQGLPEPAPVPAPLPASAPAYPRGADRG
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GSAGRVVLVTGVSRDLGRRFARALAADPSVDRVVGVDAVPPRGDIGEVSFVRADIRNPV
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCCC
IAKVIVKEDVDTVVHMSVISTPGSAGGRNTMKELNVIGTMQLLAACQKAPGLRTLVVKSS
EEHHHHHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
TTVYGASNRDPAMFTEDMEPRRVPRSGYAKDVAEVEGYVRGFARRRPDVSVTILRAANVI
CEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHC
GPQVTSPLTSYFRLPVIPTVLGFDPRLQFLHEQDLLDVLRHAIAAEVAGTFNVAGAGILM
CCHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LSQAVRRLQRPSVPLPPFAVGRVGSTLRSARVADFSPEQLGLLTYGRGVDTTRMRTELGF
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHCCC
EPALTTAGAFADFAAPLPPTGGYADRLLARVAQGLPEPAPVPAPLPASAPAYPRGADRG
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]