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The map label for this gene is lacI [H]

Identifier: 119714644

GI number: 119714644

Start: 407542

End: 408570

Strand: Direct

Name: lacI [H]

Synonym: Noca_0379

Alternate gene names: 119714644

Gene position: 407542-408570 (Clockwise)

Preceding gene: 119714643

Following gene: 119714645

Centisome position: 8.17

GC content: 75.02

Gene sequence:

>1029_bases
ATGCCGGCCCACCGCGACAAGCGCCATCCCACGATGGCCGACGTGGCGGCCCGGGCCGGGGTCTCCCACCAGACGGTGTC
ACGGGTGATCAACAACGCGCCGAGCGTCCGGCCGGAGACGGCAGCCCGGGTGGTCCAGGCGATCAGCGACCTCGGCTACC
GCCCCAACCGCTCGGCGCGGCTCCTCGCCTCGCGGCACTCCCGGCTGATCGGCGTCGCGACCTGGGGCACCAGCCACCAC
GGGCCGCAGCAGGTGCTGCTCGCGCTGGACGCGGCCGCCCGCCGCGCCGACTACCGGACCGCCGTCAGCACCCTGCACGC
GCTGACCGAGCAGGACACCCGGGACGGCGTCGAGGAGCTGCTCCAGCTCGGGGTCGAGGCGGTGGTGCTGATCATCCCGC
ACGAGTCGATGCTGCGGTTCGCGACCGAGGCCGACCTCGGGGTGCCGACCGTGGTCGTCGAGGGGGACCTCTCGCGGATG
CCACTGACCGTCGGGGTCGACAACGTCCAGGGCGGCAGCCTCGCGACCCGGCACCTGCTCGAGCTGGGCCACCGCACCGT
CGTGCACGTCGCCGGCCCGCCGGGCTGGGCCGAGGCGGCCGCCCGCGTCGACGGGTGGCGGCTGGAGCTCGAGACCTGGG
GCCGGGTGGTGCCGCCCCTGCGGTGGGGCGGCGACTGGAGTGCCCGCAGCGGGTACGACGCCGGCGTCTCCCTGGCGCGC
GATCCCGAGGTCACCGCGGTGTTCGCCGCGAACGACCAGATGGCGATGGGGGTGATCGCCGCGTTGAGGGAGGCCGGCCG
CCGGGTGCCCGACGACATCTCGGTCGTCGGGTTCGACGACCTGCCGGAGTCGGCGTACCTCGATCCGCCGCTGACCTCGG
TGCACCAGGACTTCGGGGAGCTGGGGCGCCGCGCCATGGGGCTCCTCGAGCGGGTGCTGGCCGGCGAGAAGAAGCCGACC
GCCGACCTGGTGCCGACCTCGCTGGTCGTCCGGGCGTCCACCTCGTCGCCGCGGGTGCCCGCGAACTGA

Upstream 100 bases:

>100_bases
GAAGGCGGCCACGAAGAAGGCGACCGCCAAGAAGGCGACGTAGCCCGTGGCCCCGGAGGGCCTGAGCCGCCCGGCGGATC
GGGCCGCGTAGCCTTCGACC

Downstream 100 bases:

>100_bases
CCCCCGGCGAGGCGCTCCTGGTCGGGATCGGCCCCCGGGCCCCCGGTCGTCGTCGTTTGGTCACCAACCGCAATCCGGGA
GCGCTCCCACTCGTCGTTGG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MPAHRDKRHPTMADVAARAGVSHQTVSRVINNAPSVRPETAARVVQAISDLGYRPNRSARLLASRHSRLIGVATWGTSHH
GPQQVLLALDAAARRADYRTAVSTLHALTEQDTRDGVEELLQLGVEAVVLIIPHESMLRFATEADLGVPTVVVEGDLSRM
PLTVGVDNVQGGSLATRHLLELGHRTVVHVAGPPGWAEAAARVDGWRLELETWGRVVPPLRWGGDWSARSGYDAGVSLAR
DPEVTAVFAANDQMAMGVIAALREAGRRVPDDISVVGFDDLPESAYLDPPLTSVHQDFGELGRRAMGLLERVLAGEKKPT
ADLVPTSLVVRASTSSPRVPAN

Sequences:

>Translated_342_residues
MPAHRDKRHPTMADVAARAGVSHQTVSRVINNAPSVRPETAARVVQAISDLGYRPNRSARLLASRHSRLIGVATWGTSHH
GPQQVLLALDAAARRADYRTAVSTLHALTEQDTRDGVEELLQLGVEAVVLIIPHESMLRFATEADLGVPTVVVEGDLSRM
PLTVGVDNVQGGSLATRHLLELGHRTVVHVAGPPGWAEAAARVDGWRLELETWGRVVPPLRWGGDWSARSGYDAGVSLAR
DPEVTAVFAANDQMAMGVIAALREAGRRVPDDISVVGFDDLPESAYLDPPLTSVHQDFGELGRRAMGLLERVLAGEKKPT
ADLVPTSLVVRASTSSPRVPAN
>Mature_341_residues
PAHRDKRHPTMADVAARAGVSHQTVSRVINNAPSVRPETAARVVQAISDLGYRPNRSARLLASRHSRLIGVATWGTSHHG
PQQVLLALDAAARRADYRTAVSTLHALTEQDTRDGVEELLQLGVEAVVLIIPHESMLRFATEADLGVPTVVVEGDLSRMP
LTVGVDNVQGGSLATRHLLELGHRTVVHVAGPPGWAEAAARVDGWRLELETWGRVVPPLRWGGDWSARSGYDAGVSLARD
PEVTAVFAANDQMAMGVIAALREAGRRVPDDISVVGFDDLPESAYLDPPLTSVHQDFGELGRRAMGLLERVLAGEKKPTA
DLVPTSLVVRASTSSPRVPAN

Specific function: Repressor of the lactose operon. Binds allolactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=36.7469879518072, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=32.0512820512821, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.5315315315315, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790369, Length=343, Percent_Identity=28.2798833819242, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=32.5, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787580, Length=338, Percent_Identity=27.810650887574, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=31.4569536423841, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=26.3665594855305, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI48994940, Length=294, Percent_Identity=25.1700680272109, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789456, Length=346, Percent_Identity=25.4335260115607, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787906, Length=370, Percent_Identity=27.5675675675676, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790192, Length=221, Percent_Identity=27.6018099547511, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36691; Mature: 36560

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHRDKRHPTMADVAARAGVSHQTVSRVINNAPSVRPETAARVVQAISDLGYRPNRSAR
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
LLASRHSRLIGVATWGTSHHGPQQVLLALDAAARRADYRTAVSTLHALTEQDTRDGVEEL
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLGVEAVVLIIPHESMLRFATEADLGVPTVVVEGDLSRMPLTVGVDNVQGGSLATRHLL
HHCCHHEEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHH
ELGHRTVVHVAGPPGWAEAAARVDGWRLELETWGRVVPPLRWGGDWSARSGYDAGVSLAR
HCCCCEEEEECCCCCHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCEECC
DPEVTAVFAANDQMAMGVIAALREAGRRVPDDISVVGFDDLPESAYLDPPLTSVHQDFGE
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
LGRRAMGLLERVLAGEKKPTADLVPTSLVVRASTSSPRVPAN
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
PAHRDKRHPTMADVAARAGVSHQTVSRVINNAPSVRPETAARVVQAISDLGYRPNRSAR
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
LLASRHSRLIGVATWGTSHHGPQQVLLALDAAARRADYRTAVSTLHALTEQDTRDGVEEL
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLGVEAVVLIIPHESMLRFATEADLGVPTVVVEGDLSRMPLTVGVDNVQGGSLATRHLL
HHCCHHEEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHH
ELGHRTVVHVAGPPGWAEAAARVDGWRLELETWGRVVPPLRWGGDWSARSGYDAGVSLAR
HCCCCEEEEECCCCCHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCEECC
DPEVTAVFAANDQMAMGVIAALREAGRRVPDDISVVGFDDLPESAYLDPPLTSVHQDFGE
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
LGRRAMGLLERVLAGEKKPTADLVPTSLVVRASTSSPRVPAN
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 355891; 9278503; 1107032; 4571224; 4594037; 3286877; 7498473; 2178920; 8046748; 2040302; 1923807; 3064080; 2742823; 8683581; 10647179; 8638105 [H]