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The map label for this gene is gutB [H]

Identifier: 119714375

GI number: 119714375

Start: 120754

End: 121770

Strand: Reverse

Name: gutB [H]

Synonym: Noca_0108

Alternate gene names: 119714375

Gene position: 121770-120754 (Counterclockwise)

Preceding gene: 119714378

Following gene: 119714372

Centisome position: 2.44

GC content: 76.7

Gene sequence:

>1017_bases
GTGCCCGTCGCCGCCCGGCTCCACTCCGTCGGCGACCTGCGGGTCGAGCAGGTCGCCGAGCCGCCCGCTCCCACCGTCGG
CTGGTCGACCGTCGCGGTCACCTCGGTCGGGCTCTGCGGCTCCGACCTGCACTGGTTCACCGACGGCGGCACCGGCGAGG
TCACGCTCGACCGACCGGTGGTCCCGGGCCACGAGCTCGCCGGGCGCGCACTGGACGGGCCGTACGCCGGGCGCCGGGTC
GCGATCGACCCCGCGATCCCGTGCGGGGTCTGCGAGCAGTGCCGCGCCGGGCACGGGAACCTGTGTCCCGACGTGCGCTT
CGCCGGGCACGCCGGCCTGGACGGCGGCCTCCAGGAGCGGCTGGTCTGGCCCGACCACCTGCTGTTCCCGCTCCCGGACG
ACCTCAGCGACGATGCGGGCGCGCTGCTCGAGCCGCTCGGGGTCGCGATCCACGCGGTCGGGGTGGCGCACGTCCGCCCC
GGCCACGACGTACTGGTGGTGGGCGGCGGCCCGATCGGGGTGCTCACTGCCGCGGTGGCTCGCCGTACCGGCGCCGCCCA
GGTGGTCGTGAGCGAGCCGCTCGCGCACCGGCGCGAGACCGCACGCCGCTTCGGCGCCGACGTCGCGGTCGCACCGGAGG
AGGCCTCGTCCGCGGTCGACGAGCTGACCGGCGGGCGCGGCGTCGACGTGGTCATCGAGATGGCGGGGGCGGACGACGCC
ATCGGCACCGCCGTCGCCTGCGCGCGCCCGGGCGCCCGGATCGCGCTGGGCGGCATCCCGTCGGTGCCGCTGTCCTCGTT
CCCCGCCGCACCCGCCCGGCGCAAGGGCCTGACCTTCGCGATGGTCCGCCGGATGCACGAGACCTACCCGCGCGCGATCG
AGCTCGCCACGAGCGGCATCGACCTCGACGCCCTGGTCAGCGACCGCTTCCCGCTCGCCGACGCACCCCTCGCCTTCACC
CACGCCGTCGCCCGCACCGGCGACAAGACCGTCATCGCGGTGTCCGGCACCCGTTGA

Upstream 100 bases:

>100_bases
TCGGCGCGCTGGCGCGGGTGGTGGTCGTCACCGCCGCCCGCATCTCCCGGCGCCTGAGCCACCTGCCGACCTGACCCCGG
CCGTTGGATAGGTTGGTGGC

Downstream 100 bases:

>100_bases
TCCCCGACTCGACGGGGGCGGGTTCAGTCGGCGTCGAGCGCGGTCAGCTCCTCGTCGGTGAGGTCGAGCTCGGCGGCGGC
TGCGGAGTCCTGGATCGACT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MPVAARLHSVGDLRVEQVAEPPAPTVGWSTVAVTSVGLCGSDLHWFTDGGTGEVTLDRPVVPGHELAGRALDGPYAGRRV
AIDPAIPCGVCEQCRAGHGNLCPDVRFAGHAGLDGGLQERLVWPDHLLFPLPDDLSDDAGALLEPLGVAIHAVGVAHVRP
GHDVLVVGGGPIGVLTAAVARRTGAAQVVVSEPLAHRRETARRFGADVAVAPEEASSAVDELTGGRGVDVVIEMAGADDA
IGTAVACARPGARIALGGIPSVPLSSFPAAPARRKGLTFAMVRRMHETYPRAIELATSGIDLDALVSDRFPLADAPLAFT
HAVARTGDKTVIAVSGTR

Sequences:

>Translated_338_residues
MPVAARLHSVGDLRVEQVAEPPAPTVGWSTVAVTSVGLCGSDLHWFTDGGTGEVTLDRPVVPGHELAGRALDGPYAGRRV
AIDPAIPCGVCEQCRAGHGNLCPDVRFAGHAGLDGGLQERLVWPDHLLFPLPDDLSDDAGALLEPLGVAIHAVGVAHVRP
GHDVLVVGGGPIGVLTAAVARRTGAAQVVVSEPLAHRRETARRFGADVAVAPEEASSAVDELTGGRGVDVVIEMAGADDA
IGTAVACARPGARIALGGIPSVPLSSFPAAPARRKGLTFAMVRRMHETYPRAIELATSGIDLDALVSDRFPLADAPLAFT
HAVARTGDKTVIAVSGTR
>Mature_337_residues
PVAARLHSVGDLRVEQVAEPPAPTVGWSTVAVTSVGLCGSDLHWFTDGGTGEVTLDRPVVPGHELAGRALDGPYAGRRVA
IDPAIPCGVCEQCRAGHGNLCPDVRFAGHAGLDGGLQERLVWPDHLLFPLPDDLSDDAGALLEPLGVAIHAVGVAHVRPG
HDVLVVGGGPIGVLTAAVARRTGAAQVVVSEPLAHRRETARRFGADVAVAPEEASSAVDELTGGRGVDVVIEMAGADDAI
GTAVACARPGARIALGGIPSVPLSSFPAAPARRKGLTFAMVRRMHETYPRAIELATSGIDLDALVSDRFPLADAPLAFTH
AVARTGDKTVIAVSGTR

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=327, Percent_Identity=35.7798165137615, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI1790045, Length=328, Percent_Identity=32.9268292682927, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1788073, Length=339, Percent_Identity=31.858407079646, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790718, Length=323, Percent_Identity=30.030959752322, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787863, Length=302, Percent_Identity=29.4701986754967, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788407, Length=282, Percent_Identity=30.8510638297872, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI226510992, Length=305, Percent_Identity=30.8196721311475, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788075, Length=346, Percent_Identity=26.878612716763, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1786825, Length=393, Percent_Identity=25.4452926208651, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082125, Length=245, Percent_Identity=28.9795918367347, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081918, Length=249, Percent_Identity=30.5220883534137, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=344, Percent_Identity=35.7558139534884, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17562878, Length=349, Percent_Identity=35.5300859598854, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6319955, Length=350, Percent_Identity=33.4285714285714, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322619, Length=350, Percent_Identity=33.4285714285714, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323099, Length=323, Percent_Identity=32.5077399380805, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319257, Length=346, Percent_Identity=28.0346820809249, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319258, Length=244, Percent_Identity=31.5573770491803, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17137530, Length=332, Percent_Identity=31.9277108433735, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=32.258064516129, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45550770, Length=299, Percent_Identity=27.4247491638796, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45551930, Length=299, Percent_Identity=27.4247491638796, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221457811, Length=299, Percent_Identity=27.4247491638796, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 34745; Mature: 34614

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAARLHSVGDLRVEQVAEPPAPTVGWSTVAVTSVGLCGSDLHWFTDGGTGEVTLDRPV
CCHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEECCCC
VPGHELAGRALDGPYAGRRVAIDPAIPCGVCEQCRAGHGNLCPDVRFAGHAGLDGGLQER
CCCHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCEECCCCCCCCCCHHH
LVWPDHLLFPLPDDLSDDAGALLEPLGVAIHAVGVAHVRPGHDVLVVGGGPIGVLTAAVA
EECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCHHHHHHHHH
RRTGAAQVVVSEPLAHRRETARRFGADVAVAPEEASSAVDELTGGRGVDVVIEMAGADDA
HCCCCEEEEECCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCH
IGTAVACARPGARIALGGIPSVPLSSFPAAPARRKGLTFAMVRRMHETYPRAIELATSGI
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCEEEHHHCCC
DLDALVSDRFPLADAPLAFTHAVARTGDKTVIAVSGTR
CHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
PVAARLHSVGDLRVEQVAEPPAPTVGWSTVAVTSVGLCGSDLHWFTDGGTGEVTLDRPV
CHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEECCCC
VPGHELAGRALDGPYAGRRVAIDPAIPCGVCEQCRAGHGNLCPDVRFAGHAGLDGGLQER
CCCHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCEECCCCCCCCCCHHH
LVWPDHLLFPLPDDLSDDAGALLEPLGVAIHAVGVAHVRPGHDVLVVGGGPIGVLTAAVA
EECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCHHHHHHHHH
RRTGAAQVVVSEPLAHRRETARRFGADVAVAPEEASSAVDELTGGRGVDVVIEMAGADDA
HCCCCEEEEECCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCH
IGTAVACARPGARIALGGIPSVPLSSFPAAPARRKGLTFAMVRRMHETYPRAIELATSGI
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCEEEHHHCCC
DLDALVSDRFPLADAPLAFTHAVARTGDKTVIAVSGTR
CHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]