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The map label for this gene is bfrA [H]

Identifier: 119714327

GI number: 119714327

Start: 62748

End: 63995

Strand: Reverse

Name: bfrA [H]

Synonym: Noca_0056

Alternate gene names: 119714327

Gene position: 63995-62748 (Counterclockwise)

Preceding gene: 119714328

Following gene: 119714325

Centisome position: 1.28

GC content: 68.35

Gene sequence:

>1248_bases
ATGCGGCCCCTGGTCCACTTCACTGCAGACGCCGGCTGGATCAATGACCCCCACGGCCTGACCTTCCACCGCGGCCGGTA
TCACCTGTTCCACCAGTACGTCCCCGAGAGCATGGTGTGGGCGCCCAACTGCCATTGGGGCCACGCCACGAGCTCGAACC
TCCTCACGTGGACGCGGCACCGGGTGGCGATCGCCCCTGGAGACGGGGACGACGGCATCTGGACGGGAAGCCTCGCTCTG
ACGGGCCAGGACGCCACCATCCTCTACACCTCGGTCGCCCAACCGGACCTCGGCTTGGGTCGCGTCCGCCTCGCCACCCC
GGCCGACGACTCGTGGGAGATCTGGAGCAAGGGTGACATCGTCGTCACCCCTCCCGACGAGCTCGATCTGATCGCATTCC
GCGATCCCTTCGTCGTTCGCGACGCGGCGGGCTGGCGCATGTTCATCGGCGCGGCGACGCGGGAGGGTGACGCGCTCGCC
CTCACCTACACCTCGCCGGACCTGTCGTCCTGGATCTATGAGGGCATCGCCCTCCAGAGGTCCACGAAAGAGAAGGACCC
GGTGTGGATGGGAGCGCTCTGGGAATGCCCCCAGGTCTTCGAGGTCGACGACCACTGGGTGATGGTGAGCTCCGTCTGGG
ACAACGACGTGCTGCACTATGCCGGCTACGCCCTTGGCGACCGCGACTCCTACAGCGCGGGAAAGCTGATGCCGACCGAA
TGGGGTCAACTCAGCTTCGGTGACTCCTACTACGCCCCGTCCTACTTCCTCGATGAGGACGAGCTTCCGTGTCTGATGTT
CTGGATGCGCGGCGTGAGCGATGCAGACGACGGCTGGGCGAGCTGCCTGAGCCTGCCCTATTCCTTGACCGTCCGCGATG
ACCGGCTCGTCGCCGAGCCTCACGCCGCGCTCGCCGAGGCGCGCGGCGACGCGTTGGCGGCGGGTGCCGACGCCCGCGCC
TACGACCTGGAGTGGGACCCGACCGCCAGCCAGGCCGAGCTCGTGCTGGCCTCGGACCTCGGAAAGAGCGCCACCCTGCG
CGCAACTGAGGGCAGGATCCACCTCGAGCGTCCTGGCGTCGACGCTCAGTCGATGCCCTGGCCAGGCGGCCCCGTCCGAG
TCGTCGTCGACGGTCCCGTTCTCGAGGTCTCCTGCGCCGGCGGGCTACTCGGAGGACCCATAGCCCCGGCCACCCGGTGG
GACGGACCAGCCGAGGCGTGCTCGGCCTGGAGGCTTGCTATCGACTAG

Upstream 100 bases:

>100_bases
GCGAGGAGCGCCGAGGCGAGGTGGACGACTGGGAACGCGGCAACGTGGACCAGGCGCGGGCCATCCGGCGTTCCTACGAC
GCGGAACGCGAGGGCAACTG

Downstream 100 bases:

>100_bases
TTGAATCGCGGGGTCCCGCCGTATCCGACGCCGCTACCTGTCTTCTGCTTGAGCTCGAGGCAGGCACTGCATGGCTCCGC
CTCACTTCCTGTCGCGCGGC

Product: glycosyl hydrolase family 32 protein

Products: NA

Alternate protein names: Invertase; Sucrase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MRPLVHFTADAGWINDPHGLTFHRGRYHLFHQYVPESMVWAPNCHWGHATSSNLLTWTRHRVAIAPGDGDDGIWTGSLAL
TGQDATILYTSVAQPDLGLGRVRLATPADDSWEIWSKGDIVVTPPDELDLIAFRDPFVVRDAAGWRMFIGAATREGDALA
LTYTSPDLSSWIYEGIALQRSTKEKDPVWMGALWECPQVFEVDDHWVMVSSVWDNDVLHYAGYALGDRDSYSAGKLMPTE
WGQLSFGDSYYAPSYFLDEDELPCLMFWMRGVSDADDGWASCLSLPYSLTVRDDRLVAEPHAALAEARGDALAAGADARA
YDLEWDPTASQAELVLASDLGKSATLRATEGRIHLERPGVDAQSMPWPGGPVRVVVDGPVLEVSCAGGLLGGPIAPATRW
DGPAEACSAWRLAID

Sequences:

>Translated_415_residues
MRPLVHFTADAGWINDPHGLTFHRGRYHLFHQYVPESMVWAPNCHWGHATSSNLLTWTRHRVAIAPGDGDDGIWTGSLAL
TGQDATILYTSVAQPDLGLGRVRLATPADDSWEIWSKGDIVVTPPDELDLIAFRDPFVVRDAAGWRMFIGAATREGDALA
LTYTSPDLSSWIYEGIALQRSTKEKDPVWMGALWECPQVFEVDDHWVMVSSVWDNDVLHYAGYALGDRDSYSAGKLMPTE
WGQLSFGDSYYAPSYFLDEDELPCLMFWMRGVSDADDGWASCLSLPYSLTVRDDRLVAEPHAALAEARGDALAAGADARA
YDLEWDPTASQAELVLASDLGKSATLRATEGRIHLERPGVDAQSMPWPGGPVRVVVDGPVLEVSCAGGLLGGPIAPATRW
DGPAEACSAWRLAID
>Mature_415_residues
MRPLVHFTADAGWINDPHGLTFHRGRYHLFHQYVPESMVWAPNCHWGHATSSNLLTWTRHRVAIAPGDGDDGIWTGSLAL
TGQDATILYTSVAQPDLGLGRVRLATPADDSWEIWSKGDIVVTPPDELDLIAFRDPFVVRDAAGWRMFIGAATREGDALA
LTYTSPDLSSWIYEGIALQRSTKEKDPVWMGALWECPQVFEVDDHWVMVSSVWDNDVLHYAGYALGDRDSYSAGKLMPTE
WGQLSFGDSYYAPSYFLDEDELPCLMFWMRGVSDADDGWASCLSLPYSLTVRDDRLVAEPHAALAEARGDALAAGADARA
YDLEWDPTASQAELVLASDLGKSATLRATEGRIHLERPGVDAQSMPWPGGPVRVVVDGPVLEVSCAGGLLGGPIAPATRW
DGPAEACSAWRLAID

Specific function: Hydrolysis of sucrose, raffinose, inulin and levan. Specific for the fructose moiety and the beta-anomeric configuration of the glycosidic linkages of its substrates. The enzyme released fructose from sucrose and raffinose, and the fructose polymer inulin

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=285, Percent_Identity=28.0701754385965, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 45443; Mature: 45443

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLVHFTADAGWINDPHGLTFHRGRYHLFHQYVPESMVWAPNCHWGHATSSNLLTWTRH
CCCEEEEECCCCCCCCCCCCEEECCCEEEHHHHCCCCEEECCCCCCCCCCCCCCEEEEEE
RVAIAPGDGDDGIWTGSLALTGQDATILYTSVAQPDLGLGRVRLATPADDSWEIWSKGDI
EEEEECCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEECCCCE
VVTPPDELDLIAFRDPFVVRDAAGWRMFIGAATREGDALALTYTSPDLSSWIYEGIALQR
EECCCCCEEEEEECCCEEEEECCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHCEEEEC
STKEKDPVWMGALWECPQVFEVDDHWVMVSSVWDNDVLHYAGYALGDRDSYSAGKLMPTE
CCCCCCCEEEEHHHCCCEEEECCCCEEEEEECCCCCCHHHCCEEECCCCCCCCCCCCCCC
WGQLSFGDSYYAPSYFLDEDELPCLMFWMRGVSDADDGWASCLSLPYSLTVRDDRLVAEP
CCCEECCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCEEECC
HAALAEARGDALAAGADARAYDLEWDPTASQAELVLASDLGKSATLRATEGRIHLERPGV
HHHHHHHCCCEEECCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEEEECCEEEEECCCC
DAQSMPWPGGPVRVVVDGPVLEVSCAGGLLGGPIAPATRWDGPAEACSAWRLAID
CCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHEEEEECC
>Mature Secondary Structure
MRPLVHFTADAGWINDPHGLTFHRGRYHLFHQYVPESMVWAPNCHWGHATSSNLLTWTRH
CCCEEEEECCCCCCCCCCCCEEECCCEEEHHHHCCCCEEECCCCCCCCCCCCCCEEEEEE
RVAIAPGDGDDGIWTGSLALTGQDATILYTSVAQPDLGLGRVRLATPADDSWEIWSKGDI
EEEEECCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEECCCCE
VVTPPDELDLIAFRDPFVVRDAAGWRMFIGAATREGDALALTYTSPDLSSWIYEGIALQR
EECCCCCEEEEEECCCEEEEECCCEEEEEEEECCCCCEEEEEECCCCHHHHHHHCEEEEC
STKEKDPVWMGALWECPQVFEVDDHWVMVSSVWDNDVLHYAGYALGDRDSYSAGKLMPTE
CCCCCCCEEEEHHHCCCEEEECCCCEEEEEECCCCCCHHHCCEEECCCCCCCCCCCCCCC
WGQLSFGDSYYAPSYFLDEDELPCLMFWMRGVSDADDGWASCLSLPYSLTVRDDRLVAEP
CCCEECCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCEEECC
HAALAEARGDALAAGADARAYDLEWDPTASQAELVLASDLGKSATLRATEGRIHLERPGV
HHHHHHHCCCEEECCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEEEECCEEEEECCCC
DAQSMPWPGGPVRVVVDGPVLEVSCAGGLLGGPIAPATRWDGPAEACSAWRLAID
CCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9720201; 10360571 [H]