BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is crt [H]

Identifier: 119714316

GI number: 119714316

Start: 49963

End: 50724

Strand: Direct

Name: crt [H]

Synonym: Noca_0044

Alternate gene names: 119714316

Gene position: 49963-50724 (Clockwise)

Preceding gene: 119714315

Following gene: 119714317

Centisome position: 1.0

GC content: 70.87

Gene sequence:

>762_bases
ATGCACGTAACCTGGACCGCCGACGGACACGTCGCCCGCGTCGCGCTCTGCCGACCGCCGGTCAACGCGTTCAGCCGGGA
GATGATCGCGGACCTCGAGATGGTCCTCGCAGAGGTGGAGAGCAGTGACGCTCGGGCCGTGGTCGTCACCGGGGGGTCGC
GGTTCTCCGCCGGGGTCGACGTCGGGCTGCTGGCTCAGGCCCCGCCCGAGGACGCCATTCCGCGCAACGCGTCCTTTCAG
CGCGTGTTCGACCGGATCCAGCACCATCGGCTGCCGTTCGTCGCAGCCGTGAACGGTTACGCGCTCGGCGGGGGGTGCGA
GCTCGCCATGGCCTGCGACATCCGGGTCGCCGCCCGTGACGCCTTCTTCGCGCTACCCGAGATCGGCCTCGGCGGCCTGC
CCGGTATCGGAGGCATGGCTCGCGTGCAGCGACTCGTGGGGCCGGGCAAGGCGCGCCAGCTGGTACTGACCGGCGACCGG
ATACCCGCCGAGGAGGCGTACCGGATCGGGCTGGTCGAGGAGCTCGCCGAGCCCGGCTGCGCCGAGACAGTGGCGCAGGA
GGTTGCCGAACGGATCGCGGCCCGGCCACCTCTCTCCGTCCAAGCCGGGAAACGGGCTCTCGACCAAGGTGCGGACGTCA
GTCTTGTGGCAGCGCAACAGATCGACCTTCGGTACTGCGGCGAGATCGCGGGTACCGAGGACCGCCAGGAGTCGCTCCGG
GCGTTCCTCGAGAAGCGGCCGCCCGTGGTCGTCGGCAGGTGA

Upstream 100 bases:

>100_bases
GTGCTGATCTTCTACCTGTTCATGCAGCGCCAGTTCATGGCCGGGATCACCGGGGGAGCCGTCAAGGAGTAGAGCGCAAC
GCGCCAGAGAGGATCCATGA

Downstream 100 bases:

>100_bases
GTGCCGCGCGCGGCGTCGACGACTGGTCCCGCACGCCGGACCGGGCCGCCCAGAACGAGACCTTCGGCCCACGCCGGGTC
GTCGTGAGCGATGCGCTGGT

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MHVTWTADGHVARVALCRPPVNAFSREMIADLEMVLAEVESSDARAVVVTGGSRFSAGVDVGLLAQAPPEDAIPRNASFQ
RVFDRIQHHRLPFVAAVNGYALGGGCELAMACDIRVAARDAFFALPEIGLGGLPGIGGMARVQRLVGPGKARQLVLTGDR
IPAEEAYRIGLVEELAEPGCAETVAQEVAERIAARPPLSVQAGKRALDQGADVSLVAAQQIDLRYCGEIAGTEDRQESLR
AFLEKRPPVVVGR

Sequences:

>Translated_253_residues
MHVTWTADGHVARVALCRPPVNAFSREMIADLEMVLAEVESSDARAVVVTGGSRFSAGVDVGLLAQAPPEDAIPRNASFQ
RVFDRIQHHRLPFVAAVNGYALGGGCELAMACDIRVAARDAFFALPEIGLGGLPGIGGMARVQRLVGPGKARQLVLTGDR
IPAEEAYRIGLVEELAEPGCAETVAQEVAERIAARPPLSVQAGKRALDQGADVSLVAAQQIDLRYCGEIAGTEDRQESLR
AFLEKRPPVVVGR
>Mature_253_residues
MHVTWTADGHVARVALCRPPVNAFSREMIADLEMVLAEVESSDARAVVVTGGSRFSAGVDVGLLAQAPPEDAIPRNASFQ
RVFDRIQHHRLPFVAAVNGYALGGGCELAMACDIRVAARDAFFALPEIGLGGLPGIGGMARVQRLVGPGKARQLVLTGDR
IPAEEAYRIGLVEELAEPGCAETVAQEVAERIAARPPLSVQAGKRALDQGADVSLVAAQQIDLRYCGEIAGTEDRQESLR
AFLEKRPPVVVGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=36.734693877551, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=35.1351351351351, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=36.1256544502618, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=260, Percent_Identity=29.2307692307692, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4502327, Length=234, Percent_Identity=32.9059829059829, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI31542718, Length=233, Percent_Identity=29.1845493562232, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=232, Percent_Identity=28.0172413793103, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=28.0172413793103, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=210, Percent_Identity=27.6190476190476, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI62530384, Length=240, Percent_Identity=25, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI150378541, Length=140, Percent_Identity=35, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=233, Percent_Identity=23.175965665236, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI221136753, Length=233, Percent_Identity=23.175965665236, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI295842266, Length=238, Percent_Identity=23.9495798319328, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=233, Percent_Identity=23.175965665236, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI37594469, Length=196, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=233, Percent_Identity=39.0557939914163, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=33.7209302325581, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=34.0782122905028, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=239, Percent_Identity=26.3598326359833, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788682, Length=186, Percent_Identity=32.258064516129, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=32.5396825396825, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=27.734375, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=38.1147540983607, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=36.734693877551, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=28.695652173913, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558304, Length=214, Percent_Identity=30.3738317757009, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=219, Percent_Identity=28.310502283105, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=215, Percent_Identity=30.6976744186047, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=220, Percent_Identity=30.4545454545455, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=228, Percent_Identity=29.8245614035088, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=27.34375, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=31.0204081632653, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=214, Percent_Identity=28.9719626168224, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=29.2307692307692, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=195, Percent_Identity=29.2307692307692, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=25.4980079681275, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=203, Percent_Identity=27.0935960591133, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=35.6846473029046, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=35.6846473029046, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=34, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=30.8411214953271, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=29.4354838709677, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921018, Length=168, Percent_Identity=29.7619047619048, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921000, Length=164, Percent_Identity=38.4146341463415, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583077, Length=164, Percent_Identity=38.4146341463415, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583079, Length=164, Percent_Identity=38.4146341463415, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583165, Length=170, Percent_Identity=31.1764705882353, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571729, Length=177, Percent_Identity=29.9435028248588, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=177, Percent_Identity=29.9435028248588, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550169, Length=240, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24654903, Length=207, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=30.8056872037915, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 26882; Mature: 26882

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTWTADGHVARVALCRPPVNAFSREMIADLEMVLAEVESSDARAVVVTGGSRFSAGVD
CEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
VGLLAQAPPEDAIPRNASFQRVFDRIQHHRLPFVAAVNGYALGGGCELAMACDIRVAARD
EEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCEEECCCCEEEEEECEEEEHHH
AFFALPEIGLGGLPGIGGMARVQRLVGPGKARQLVLTGDRIPAEEAYRIGLVEELAEPGC
HHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCH
AETVAQEVAERIAARPPLSVQAGKRALDQGADVSLVAAQQIDLRYCGEIAGTEDRQESLR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEHHHHCHHHHHCCCCCCHHHHHHH
AFLEKRPPVVVGR
HHHHHCCCEEECC
>Mature Secondary Structure
MHVTWTADGHVARVALCRPPVNAFSREMIADLEMVLAEVESSDARAVVVTGGSRFSAGVD
CEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
VGLLAQAPPEDAIPRNASFQRVFDRIQHHRLPFVAAVNGYALGGGCELAMACDIRVAARD
EEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCEEECCCCEEEEEECEEEEHHH
AFFALPEIGLGGLPGIGGMARVQRLVGPGKARQLVLTGDRIPAEEAYRIGLVEELAEPGC
HHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCH
AETVAQEVAERIAARPPLSVQAGKRALDQGADVSLVAAQQIDLRYCGEIAGTEDRQESLR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEHHHHCHHHHHCCCCCCHHHHHHH
AFLEKRPPVVVGR
HHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]