BacMap

Definition	Paracoccus denitrificans PD1222 chromosome 2, complete sequence.
Accession	NC_008687
Length	1,730,097

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The map label for this gene is echA8 [H]

Identifier: 119387218

GI number: 119387218

Start: 1728790

End: 1729566

Strand: Direct

Name: echA8 [H]

Synonym: Pden_4513

Alternate gene names: 119387218

Gene position: 1728790-1729566 (Clockwise)

Preceding gene: 119387217

Following gene: 119387219

Centisome position: 99.92

GC content: 66.28

Gene sequence:

>777_bases
ATGGCCTACCAGACCCTCACCGTCGAGATCGACGACTATATCGCGCTGATCCGGCTGAACCGGCCCGAGGCGCTGAATGC
ACTGAACTCGCAGCTGCTGAGCGAGCTGGGCGATGCGCTGGCCGATGCCGACCGGAACGAAAAGGTCCGCTGCATCGTGC
TGACCGGCAGCGAAAAAGCCTTTGCCGCCGGCGCCGACATCAAGGAGATGTCGGCCAAGAGCTTCGTCGACGTCTATACC
GACGACCTGTTCGGCGCCGAGATCGACCGCATCACCGCGACGCGCAAGCCGATCATCGCCGCGGTCGCGGGCTATGCGCT
TGGCGGGGGATGCGAGCTGGCCATGGCCTGCGACTTCATCCTCTGCGCCGACAACGCCAAGTTCGGCCAGCCCGAGATCA
ATCTGGGCGTCATCGCCGGCATCGGCGGCACCCAGCGCCTGACCCGCTTTGTCGGCAAGTCCAAGTCGATGGAGATGCAC
CTGACCGGCCGCTTCATGGATGCGGCCGAGGCCGAGCGCTCGGGCCTGGTCAGCCGGGTCGTGCCGGCCGCCAAGCTGAT
CCCCGAAGCGCTGGCGGCGGCGAAGAAGATCGCCGAAAAGGGCCAGATCGCCGTCATGGCCGCCAAGGAGGCGGTGAACC
GCGCCTATGAGACCACGCTGCGCGAGGGCATCCTGTTCGAGCGCCGCAGCTTCCAGGCGCTGTTCGGGACCGAGGACCAG
AAAGAGGGCATGGCCGCCTTTCTGGAAAAGCGCGAGCCGCAATTCCGCGACCGCTGA

Upstream 100 bases:

>100_bases
CCTTCCCCACGCCTGAGCCGGTGATATCGGCAGGCGCTGGCCTTGGCCTCGCGCATTTGTTACGAGTCGCGGTCAGCTTG
TTTGCGAAAGGCAGGAGTCG

Downstream 100 bases:

>100_bases
TTTCGCGCTTGGCATCCGGCCCGGATCGCTGTATAGGCCCGGGCTTACATGCGTGTGGGCCCGCTTAAAGGCAAGAATCA
TGACCGTCCGTTCCCGGACG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYQTLTVEIDDYIALIRLNRPEALNALNSQLLSELGDALADADRNEKVRCIVLTGSEKAFAAGADIKEMSAKSFVDVYT
DDLFGAEIDRITATRKPIIAAVAGYALGGGCELAMACDFILCADNAKFGQPEINLGVIAGIGGTQRLTRFVGKSKSMEMH
LTGRFMDAAEAERSGLVSRVVPAAKLIPEALAAAKKIAEKGQIAVMAAKEAVNRAYETTLREGILFERRSFQALFGTEDQ
KEGMAAFLEKREPQFRDR

Sequences:

>Translated_258_residues
MAYQTLTVEIDDYIALIRLNRPEALNALNSQLLSELGDALADADRNEKVRCIVLTGSEKAFAAGADIKEMSAKSFVDVYT
DDLFGAEIDRITATRKPIIAAVAGYALGGGCELAMACDFILCADNAKFGQPEINLGVIAGIGGTQRLTRFVGKSKSMEMH
LTGRFMDAAEAERSGLVSRVVPAAKLIPEALAAAKKIAEKGQIAVMAAKEAVNRAYETTLREGILFERRSFQALFGTEDQ
KEGMAAFLEKREPQFRDR
>Mature_257_residues
AYQTLTVEIDDYIALIRLNRPEALNALNSQLLSELGDALADADRNEKVRCIVLTGSEKAFAAGADIKEMSAKSFVDVYTD
DLFGAEIDRITATRKPIIAAVAGYALGGGCELAMACDFILCADNAKFGQPEINLGVIAGIGGTQRLTRFVGKSKSMEMHL
TGRFMDAAEAERSGLVSRVVPAAKLIPEALAAAKKIAEKGQIAVMAAKEAVNRAYETTLREGILFERRSFQALFGTEDQK
EGMAAFLEKREPQFRDR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=52.8225806451613, Blast_Score=253, Evalue=8e-68,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=30.4511278195489, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=31.0483870967742, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=33.5078534031414, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=35.6, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=35.0574712643678, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=32, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI213417737, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI157694516, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=28.3783783783784, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=28.3783783783784, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=28.3783783783784, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI37594469, Length=200, Percent_Identity=26.5, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI37594471, Length=199, Percent_Identity=27.1356783919598, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI62530384, Length=186, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=21.9230769230769, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=21.9230769230769, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI221136756, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=21.455938697318, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=21.455938697318, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=264, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4757966, Length=217, Percent_Identity=23.5023041474654, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI51538581, Length=217, Percent_Identity=23.5023041474654, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=44.140625, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.1538461538462, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=34.6153846153846, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=34.3873517786561, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=31.6205533596838, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=37.3737373737374, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=32.3232323232323, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=53.469387755102, Blast_Score=263, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=49.5867768595041, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=34.6491228070175, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17534483, Length=264, Percent_Identity=28.7878787878788, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=255, Percent_Identity=31.3725490196078, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=218, Percent_Identity=31.651376146789, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540306, Length=233, Percent_Identity=30.0429184549356, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=239, Percent_Identity=32.2175732217573, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=239, Percent_Identity=32.2175732217573, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=239, Percent_Identity=32.2175732217573, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=233, Percent_Identity=27.4678111587983, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=231, Percent_Identity=29.004329004329, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=231, Percent_Identity=29.004329004329, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=28.7179487179487, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=56.5573770491803, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=56.5573770491803, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=36.2549800796813, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922422, Length=242, Percent_Identity=30.5785123966942, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920382, Length=276, Percent_Identity=32.2463768115942, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=30.2325581395349, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650670, Length=215, Percent_Identity=32.093023255814, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=34.2245989304813, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=34.2245989304813, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=34.2245989304813, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=27.807486631016, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571730, Length=235, Percent_Identity=26.8085106382979, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=235, Percent_Identity=26.8085106382979, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921018, Length=185, Percent_Identity=30.8108108108108, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583165, Length=149, Percent_Identity=30.8724832214765, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27974; Mature: 27843

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQTLTVEIDDYIALIRLNRPEALNALNSQLLSELGDALADADRNEKVRCIVLTGSEKA
CCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
FAAGADIKEMSAKSFVDVYTDDLFGAEIDRITATRKPIIAAVAGYALGGGCELAMACDFI
EECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHCCEE
LCADNAKFGQPEINLGVIAGIGGTQRLTRFVGKSKSMEMHLTGRFMDAAEAERSGLVSRV
EEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHH
VPAAKLIPEALAAAKKIAEKGQIAVMAAKEAVNRAYETTLREGILFERRSFQALFGTEDQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KEGMAAFLEKREPQFRDR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYQTLTVEIDDYIALIRLNRPEALNALNSQLLSELGDALADADRNEKVRCIVLTGSEKA
CCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
FAAGADIKEMSAKSFVDVYTDDLFGAEIDRITATRKPIIAAVAGYALGGGCELAMACDFI
EECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHCCEE
LCADNAKFGQPEINLGVIAGIGGTQRLTRFVGKSKSMEMHLTGRFMDAAEAERSGLVSRV
EEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHH
VPAAKLIPEALAAAKKIAEKGQIAVMAAKEAVNRAYETTLREGILFERRSFQALFGTEDQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KEGMAAFLEKREPQFRDR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]