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The map label for this gene is ydeM [C]

Identifier: 119387052

GI number: 119387052

Start: 1532148

End: 1533389

Strand: Direct

Name: ydeM [C]

Synonym: Pden_4347

Alternate gene names: 119387052

Gene position: 1532148-1533389 (Clockwise)

Preceding gene: 119387051

Following gene: 119387053

Centisome position: 88.56

GC content: 67.95

Gene sequence:

>1242_bases
ATGGAAAGCCGCGAAGCGAAATCTCTGGACCTGATCGTCAAGGTGGCCGAGCGCTGCAACATTGCCTGCACCTATTGCTA
TTTCTTTTTCGGCGGCGATGAGAGTCACAAGTTCCATCCGCCCTATGTCAACGAGGAAACCGCCGCCGAAATCGAAGCCT
TCCTGGACCGCATCACCGCCGACGGCGCGGTCGAGAACCTGATGATCTATTTCCACGGCGGCGAGCCGATGATGGCGCCC
AAGCCGGTGATCGCCCGCATCGCCCGCGCCGCCCGCCGCCTGTCCGAAAGCGGCCGGGTGGCCGATCTGGGCCTGTCGAT
GCAGACCAACGGCATGCTGGTCAACGACGCCTGGATCCGCTTTTGCGCGGATCTGGGGATCGGCGTCGGCGTCAGCCTGG
ACGGCAACGAGGCGGCGAACGACCGCTTTCGCATCGACAAGAAGGGGCGCGGCACCTATCGCCGCGCGGTGCAGGGCTTT
CGCCGCCTCGCGGGCGAGAACGGGGCCGGCATCGACCTGCATCCCGGCCTGCTGAGCGTGATCGACCCCGAGGGCGACGG
CGCCCGGCGCCTGGAGCATTTCTCGGGCGAGATCGGCGCGCAGCGGATGAACTTCCTGCATCCCGACGGCGGCCACGACC
AGGGCATCGCGCCGGCACAGGTGGCCGGGGTCGAGAATTTCATGCTCGGCGTGTTCCGCGCCTGGGTGCGGCGGCGCGAC
AGCGGGCTGCGCGTGCGCTTCATCAAGGATCTGCTCGACACCCTGCTCAGCGACGAGGGGGCGCGTACTTGCCCGGATTC
GGCACGCAGACTTTCGCGCATCCTGACGATTTCCTCGAACGGGATGCTGGGCGCCGAGGATACCGCGCGCACGCTGGACG
CACGTTTCCGCGATATCGGCCATGTGCGCGATTTCGACAGCGTGGCGCAGATCTTCGCGACGCCGGCACTGGTTGAGATG
CAGCAGGCCGCCGCCACGCCGCCGGCCGCCTGCGCCGCCTGCCCATGGTGGGGCACCTGCCGGGGCGGCACCTTCACCAA
CCGCTATTCGGCGCAGAACGGCTTCGACAATCCCAGCGTCTATTGCGATGCGCTCAAGGCGTTCTTCGAAGAGGTCTCCG
CCTGGCTGGTGCGCGGCGGCATCCCGGTCGAGGATATCGAGCGGCGGCTGATCCTGGCCGGCTCGGCCCCCGAGGCGGTC
CTGGGCGCCGCGCGGGCAGAACCCGAGCCCCTGCGCGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGGCGACGGCCATTCCAAGACCACGACCACGGGCGAGTGCAAGTAACGGCCCGAGCAGCGCCCGGCCGGCATGAAC
GTCACCAAGGAGGATCGTCG

Downstream 100 bases:

>100_bases
TCGCGCATGAGCGGCCGCCGCATTGCCCTTGCACCGCAGGGGCGACTAGGCTGGATGTCGTGGACAATGGCGGGGACAAG
GCGAATGGGGATGCTGACGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MESREAKSLDLIVKVAERCNIACTYCYFFFGGDESHKFHPPYVNEETAAEIEAFLDRITADGAVENLMIYFHGGEPMMAP
KPVIARIARAARRLSESGRVADLGLSMQTNGMLVNDAWIRFCADLGIGVGVSLDGNEAANDRFRIDKKGRGTYRRAVQGF
RRLAGENGAGIDLHPGLLSVIDPEGDGARRLEHFSGEIGAQRMNFLHPDGGHDQGIAPAQVAGVENFMLGVFRAWVRRRD
SGLRVRFIKDLLDTLLSDEGARTCPDSARRLSRILTISSNGMLGAEDTARTLDARFRDIGHVRDFDSVAQIFATPALVEM
QQAAATPPAACAACPWWGTCRGGTFTNRYSAQNGFDNPSVYCDALKAFFEEVSAWLVRGGIPVEDIERRLILAGSAPEAV
LGAARAEPEPLRA

Sequences:

>Translated_413_residues
MESREAKSLDLIVKVAERCNIACTYCYFFFGGDESHKFHPPYVNEETAAEIEAFLDRITADGAVENLMIYFHGGEPMMAP
KPVIARIARAARRLSESGRVADLGLSMQTNGMLVNDAWIRFCADLGIGVGVSLDGNEAANDRFRIDKKGRGTYRRAVQGF
RRLAGENGAGIDLHPGLLSVIDPEGDGARRLEHFSGEIGAQRMNFLHPDGGHDQGIAPAQVAGVENFMLGVFRAWVRRRD
SGLRVRFIKDLLDTLLSDEGARTCPDSARRLSRILTISSNGMLGAEDTARTLDARFRDIGHVRDFDSVAQIFATPALVEM
QQAAATPPAACAACPWWGTCRGGTFTNRYSAQNGFDNPSVYCDALKAFFEEVSAWLVRGGIPVEDIERRLILAGSAPEAV
LGAARAEPEPLRA
>Mature_413_residues
MESREAKSLDLIVKVAERCNIACTYCYFFFGGDESHKFHPPYVNEETAAEIEAFLDRITADGAVENLMIYFHGGEPMMAP
KPVIARIARAARRLSESGRVADLGLSMQTNGMLVNDAWIRFCADLGIGVGVSLDGNEAANDRFRIDKKGRGTYRRAVQGF
RRLAGENGAGIDLHPGLLSVIDPEGDGARRLEHFSGEIGAQRMNFLHPDGGHDQGIAPAQVAGVENFMLGVFRAWVRRRD
SGLRVRFIKDLLDTLLSDEGARTCPDSARRLSRILTISSNGMLGAEDTARTLDARFRDIGHVRDFDSVAQIFATPALVEM
QQAAATPPAACAACPWWGTCRGGTFTNRYSAQNGFDNPSVYCDALKAFFEEVSAWLVRGGIPVEDIERRLILAGSAPEAV
LGAARAEPEPLRA

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=383, Percent_Identity=22.976501305483, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994973, Length=410, Percent_Identity=21.7073170731707, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 45065; Mature: 45065

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESREAKSLDLIVKVAERCNIACTYCYFFFGGDESHKFHPPYVNEETAAEIEAFLDRITA
CCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DGAVENLMIYFHGGEPMMAPKPVIARIARAARRLSESGRVADLGLSMQTNGMLVNDAWIR
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCEEECCCEEEHHHHHH
FCADLGIGVGVSLDGNEAANDRFRIDKKGRGTYRRAVQGFRRLAGENGAGIDLHPGLLSV
HHHHCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEE
IDPEGDGARRLEHFSGEIGAQRMNFLHPDGGHDQGIAPAQVAGVENFMLGVFRAWVRRRD
ECCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SGLRVRFIKDLLDTLLSDEGARTCPDSARRLSRILTISSNGMLGAEDTARTLDARFRDIG
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHC
HVRDFDSVAQIFATPALVEMQQAAATPPAACAACPWWGTCRGGTFTNRYSAQNGFDNPSV
CCCCHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
YCDALKAFFEEVSAWLVRGGIPVEDIERRLILAGSAPEAVLGAARAEPEPLRA
HHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MESREAKSLDLIVKVAERCNIACTYCYFFFGGDESHKFHPPYVNEETAAEIEAFLDRITA
CCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DGAVENLMIYFHGGEPMMAPKPVIARIARAARRLSESGRVADLGLSMQTNGMLVNDAWIR
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCEEECCCEEEHHHHHH
FCADLGIGVGVSLDGNEAANDRFRIDKKGRGTYRRAVQGFRRLAGENGAGIDLHPGLLSV
HHHHCCCCEEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEECCCEEEE
IDPEGDGARRLEHFSGEIGAQRMNFLHPDGGHDQGIAPAQVAGVENFMLGVFRAWVRRRD
ECCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
SGLRVRFIKDLLDTLLSDEGARTCPDSARRLSRILTISSNGMLGAEDTARTLDARFRDIG
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHC
HVRDFDSVAQIFATPALVEMQQAAATPPAACAACPWWGTCRGGTFTNRYSAQNGFDNPSV
CCCCHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
YCDALKAFFEEVSAWLVRGGIPVEDIERRLILAGSAPEAVLGAARAEPEPLRA
HHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA