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The map label for this gene is ansA [H]

Identifier: 119386949

GI number: 119386949

Start: 1409413

End: 1410396

Strand: Direct

Name: ansA [H]

Synonym: Pden_4244

Alternate gene names: 119386949

Gene position: 1409413-1410396 (Clockwise)

Preceding gene: 119386948

Following gene: 119386953

Centisome position: 81.46

GC content: 72.46

Gene sequence:

>984_bases
TTGCTGAACCATGCGGCTCATGTCGTGACCGGGACCGGACAGATCCCGCTGCTGGCGGTGCTCGCCACCGGCGGCACCAT
CGCCAGCAGCCGCGGCGCGGACGGCGCCGCAAGCCCCAGCCTGAGGGCCGAGGATCTTTTGGCGCTGCTGCCGTCCATGG
CGGTCGATTTGCGTCCGCAGGAGGTGCTGGCCAAGGACTCCGCCAGCCTGACGCTGGCCGACATGCAGCGCATCAGCGAT
GCGGTGGCGACCGAACTGGCCGATCCCCGCATCGCCGGCATCGTCGCGTTGCACGGCACCGATGCGATGGAAGAGACCGC
GCTGCTGGTGCAATTGCAGCATGCGCCGCGCAAGCCGGTGGTGTTCACGGGGGCGCAATTCGCCTCGGACCATCCGCGCT
CGGACGGGGCGCGCAACCTGACGGATGCCGTCCGCACCGCATTGCAGCCGCCGCGCCCGGGGGGCGTGACGCTGGCCTTC
GGCGGCCGGGTGCTGCCGGTCTGGGGGCTTTACAAGTTCACCTCGGACGCGCCCGACGCCTTCCGCCGCGTGGCCGACGA
GGGCGCGACCGTCACCCGTCCGCTGCCGGCCCCGGTCGACGGCTTGCGCGTCGACATCGTCGCCACCCATCCCGGCAGCG
ACGGGCTGCATCTCGAGGCCAGCCTTGCCGCCGGCGCGCAGGGCATCGTGGTCTCGGCCCTGGGTTCGGGCAATGCCACG
CCCGAACTGGTCGCAGCCATCTCGCGCGCCCGGGCGCAAGGGGTGATGGTGGTGGTGTCCAGCCGCGTGCCCGAAGGCCT
GCTGTCGCCGATCTATGGCGGCGGCGGGGGCGGCCATGACATGCGGCGCGCGGGCGCCATCCATGCCCGGCTGCTGCGGC
CCGGGCAGGCGCGCATCCTGCTAGCGGCAATGCTGGCCAATGGCTGCTCCGAGGCCGAGATCGCCCGCGCCTTCGAAACC
GCGCCCGAGACCGTGCCGGCGTGA

Upstream 100 bases:

>100_bases
GACGGCTTGACCCCGTCGCCGGCTGTGGTTATCGCGAGCTGTCAGACAGATTGCGGCAAGGCGGGCGCGCCCGTCCGGCC
AAGGATCTGCAAGGGAGGGA

Downstream 100 bases:

>100_bases
AGCCGCCCGCACGGAAGCCAAGGCGATCCGCGATGCGAATCAAAACTCGAATTTTTCCACGAGATTCCGCCGCCCGCGGG
TGAAGGCGTCGGCGACCAGC

Product: asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLNHAAHVVTGTGQIPLLAVLATGGTIASSRGADGAASPSLRAEDLLALLPSMAVDLRPQEVLAKDSASLTLADMQRISD
AVATELADPRIAGIVALHGTDAMEETALLVQLQHAPRKPVVFTGAQFASDHPRSDGARNLTDAVRTALQPPRPGGVTLAF
GGRVLPVWGLYKFTSDAPDAFRRVADEGATVTRPLPAPVDGLRVDIVATHPGSDGLHLEASLAAGAQGIVVSALGSGNAT
PELVAAISRARAQGVMVVVSSRVPEGLLSPIYGGGGGGHDMRRAGAIHARLLRPGQARILLAAMLANGCSEAEIARAFET
APETVPA

Sequences:

>Translated_327_residues
MLNHAAHVVTGTGQIPLLAVLATGGTIASSRGADGAASPSLRAEDLLALLPSMAVDLRPQEVLAKDSASLTLADMQRISD
AVATELADPRIAGIVALHGTDAMEETALLVQLQHAPRKPVVFTGAQFASDHPRSDGARNLTDAVRTALQPPRPGGVTLAF
GGRVLPVWGLYKFTSDAPDAFRRVADEGATVTRPLPAPVDGLRVDIVATHPGSDGLHLEASLAAGAQGIVVSALGSGNAT
PELVAAISRARAQGVMVVVSSRVPEGLLSPIYGGGGGGHDMRRAGAIHARLLRPGQARILLAAMLANGCSEAEIARAFET
APETVPA
>Mature_327_residues
MLNHAAHVVTGTGQIPLLAVLATGGTIASSRGADGAASPSLRAEDLLALLPSMAVDLRPQEVLAKDSASLTLADMQRISD
AVATELADPRIAGIVALHGTDAMEETALLVQLQHAPRKPVVFTGAQFASDHPRSDGARNLTDAVRTALQPPRPGGVTLAF
GGRVLPVWGLYKFTSDAPDAFRRVADEGATVTRPLPAPVDGLRVDIVATHPGSDGLHLEASLAAGAQGIVVSALGSGNAT
PELVAAISRARAQGVMVVVSSRVPEGLLSPIYGGGGGGHDMRRAGAIHARLLRPGQARILLAAMLANGCSEAEIARAFET
APETVPA

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788065, Length=301, Percent_Identity=27.906976744186, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789327, Length=305, Percent_Identity=28.8524590163934, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=336, Percent_Identity=27.9761904761905, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323187, Length=336, Percent_Identity=27.9761904761905, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323186, Length=336, Percent_Identity=27.9761904761905, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323184, Length=336, Percent_Identity=27.9761904761905, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320527, Length=337, Percent_Identity=27.299703264095, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 33428; Mature: 33428

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNHAAHVVTGTGQIPLLAVLATGGTIASSRGADGAASPSLRAEDLLALLPSMAVDLRPQ
CCCCCEEEEECCCCCEEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
EVLAKDSASLTLADMQRISDAVATELADPRIAGIVALHGTDAMEETALLVQLQHAPRKPV
HHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHEEEECCCCCCCE
VFTGAQFASDHPRSDGARNLTDAVRTALQPPRPGGVTLAFGGRVLPVWGLYKFTSDAPDA
EEECCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEEEHEEEHHCCCCHHH
FRRVADEGATVTRPLPAPVDGLRVDIVATHPGSDGLHLEASLAAGAQGIVVSALGSGNAT
HHHHHHCCCEEECCCCCCCCCEEEEEEEECCCCCCEEEEEEHHCCCCCEEEEECCCCCCC
PELVAAISRARAQGVMVVVSSRVPEGLLSPIYGGGGGGHDMRRAGAIHARLLRPGQARIL
HHHHHHHHHHHHCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
LAAMLANGCSEAEIARAFETAPETVPA
HHHHHHCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLNHAAHVVTGTGQIPLLAVLATGGTIASSRGADGAASPSLRAEDLLALLPSMAVDLRPQ
CCCCCEEEEECCCCCEEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
EVLAKDSASLTLADMQRISDAVATELADPRIAGIVALHGTDAMEETALLVQLQHAPRKPV
HHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHEEEECCCCCCCE
VFTGAQFASDHPRSDGARNLTDAVRTALQPPRPGGVTLAFGGRVLPVWGLYKFTSDAPDA
EEECCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCEEEEEHEEEHHCCCCHHH
FRRVADEGATVTRPLPAPVDGLRVDIVATHPGSDGLHLEASLAAGAQGIVVSALGSGNAT
HHHHHHCCCEEECCCCCCCCCEEEEEEEECCCCCCEEEEEEHHCCCCCEEEEECCCCCCC
PELVAAISRARAQGVMVVVSSRVPEGLLSPIYGGGGGGHDMRRAGAIHARLLRPGQARIL
HHHHHHHHHHHHCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
LAAMLANGCSEAEIARAFETAPETVPA
HHHHHHCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]