Click here to switch to the map view.

The map label for this gene is azoR2 [H]

Identifier: 119386870

GI number: 119386870

Start: 1321888

End: 1322436

Strand: Direct

Name: azoR2 [H]

Synonym: Pden_4165

Alternate gene names: 119386870

Gene position: 1321888-1322436 (Clockwise)

Preceding gene: 119386868

Following gene: 119386879

Centisome position: 76.41

GC content: 65.94

Gene sequence:

>549_bases
GTGACCATCCTGCATATCGACAGCAGCATCACCGGCGAGAACAGCGTCAGCCGCATCGTCAGCCAGGCGATCATCGACCG
GCTGGCCGAGGCGCAGCCCGGCATCCCCGTCATTCGCCGCGATCTTGCCAGCGCGCCGCTGCCGCACCTGACGCTGGATG
CGCTTGGCGACACTTCGGTGCTGGACGAGTTCCTGGCCGCCGATACGGTGGTGATCGGCGCGCCGATGTATAACTTCACC
CTGCCCAGCCAGCTCAAGGCATGGATCGACCGCATCGTCGTTGCCGGCAAGACCTTCCGCTATACCGCCGAAGGGCCGGA
GGGGCTGGCCAAGGGCAAGCGCGTGATCGTCGCCCTGGCGCGCGGCGGTTTTTACAGCGACGAGGCCAGGATCGCGCTGG
AGCATCTGGAAAGCTATCTGCGCTCGGTCTTTGCCTTCATCGGCATCGAGGCCGAATTCGTGATGGCCGATGGCGTGAGC
ATCGGTCCCGAACAGCGCCAGGCCGCCATCGACCAGGCGCTTGGCCAGACCCGGCAGCTTGCCGCCTGA

Upstream 100 bases:

>100_bases
CAGCCATGTAAGTGCGGCACAGGATTGTGCCTTCTTGCGGGGGGTTCCCCGATGGTTACTTAGGGGATCTCAGTATAGAT
TCCATACCTAGGAGACAAGA

Downstream 100 bases:

>100_bases
GCGCGCCGCCGCCTGTCCTTCACGGCAGGCGGCTGATCGCCGGCCAGTAATCGGCCAGCCACAGCAGGAACGCCGCCGCC
CACAGGACCGCCGCGACCAG

Product: (Acyl-carrier-protein) phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MTILHIDSSITGENSVSRIVSQAIIDRLAEAQPGIPVIRRDLASAPLPHLTLDALGDTSVLDEFLAADTVVIGAPMYNFT
LPSQLKAWIDRIVVAGKTFRYTAEGPEGLAKGKRVIVALARGGFYSDEARIALEHLESYLRSVFAFIGIEAEFVMADGVS
IGPEQRQAAIDQALGQTRQLAA

Sequences:

>Translated_182_residues
MTILHIDSSITGENSVSRIVSQAIIDRLAEAQPGIPVIRRDLASAPLPHLTLDALGDTSVLDEFLAADTVVIGAPMYNFT
LPSQLKAWIDRIVVAGKTFRYTAEGPEGLAKGKRVIVALARGGFYSDEARIALEHLESYLRSVFAFIGIEAEFVMADGVS
IGPEQRQAAIDQALGQTRQLAA
>Mature_181_residues
TILHIDSSITGENSVSRIVSQAIIDRLAEAQPGIPVIRRDLASAPLPHLTLDALGDTSVLDEFLAADTVVIGAPMYNFTL
PSQLKAWIDRIVVAGKTFRYTAEGPEGLAKGKRVIVALARGGFYSDEARIALEHLESYLRSVFAFIGIEAEFVMADGVSI
GPEQRQAAIDQALGQTRQLAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=182, Percent_Identity=35.1648351648352, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 19576; Mature: 19444

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILHIDSSITGENSVSRIVSQAIIDRLAEAQPGIPVIRRDLASAPLPHLTLDALGDTSV
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEHHHCCCHHH
LDEFLAADTVVIGAPMYNFTLPSQLKAWIDRIVVAGKTFRYTAEGPEGLAKGKRVIVALA
HHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCEEEEEEE
RGGFYSDEARIALEHLESYLRSVFAFIGIEAEFVMADGVSIGPEQRQAAIDQALGQTRQL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
AA
CC
>Mature Secondary Structure 
TILHIDSSITGENSVSRIVSQAIIDRLAEAQPGIPVIRRDLASAPLPHLTLDALGDTSV
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEHHHCCCHHH
LDEFLAADTVVIGAPMYNFTLPSQLKAWIDRIVVAGKTFRYTAEGPEGLAKGKRVIVALA
HHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCEEEEEEE
RGGFYSDEARIALEHLESYLRSVFAFIGIEAEFVMADGVSIGPEQRQAAIDQALGQTRQL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]