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The map label for this gene is yfkN [H]

Identifier: 119386859

GI number: 119386859

Start: 1309716

End: 1311410

Strand: Direct

Name: yfkN [H]

Synonym: Pden_4154

Alternate gene names: 119386859

Gene position: 1309716-1311410 (Clockwise)

Preceding gene: 119386858

Following gene: 119386860

Centisome position: 75.7

GC content: 64.9

Gene sequence:

>1695_bases
ATGATTACCCGACGCGAGTTCTTCCAGGCCGCCATGGCCGCCTCGGCACTGGTCGGGGCCTCGGGCTTCGGAACCTGGTC
GCGGGTGGCCGCGCAGCAGGCGCTGACCCAGGACCAGCTGCTGCAATTCGACGATTTCGGCAATGTGACGCTGATCCATG
TCACCGACATCCACGCCCAGCTGAAGCCGATCTACTTCCGCGAGCCCGAGATCAATATCGGCGTGGGCGATGCCAAGGGC
CAGGTGCCGCATGTCACCGGCGCCGCGTTCCAGCAGATGTTCGGCATCGCGCCGGGCTCGGCCGACGCCTATGCGCTGAG
CTACCCGGATTTCGAGGCGCTGGCCCGCGGCTATGGCCGGATGGGCGGCATGGACCGCGTCGCCACCGTGGTCAAGGCGA
TCCGGGCCGCCCGGCCCGACTCGATCCTGCTGGATGGCGGCGACACCTGGCACGGCTCGATGACCTCGTTCCTGACCAAG
GGGCAGGACATGGTCAACGTGATGAACGCGCTTGGCGTCGACGCCATGACCAGCCACTGGGAATGGACCTATGGCACCGA
ACGGGTCAAGGAGATCGTCGAGACGCAACTGAAGTTCCCCTTTCTCGGCGCGAACATCTTCGACGCGGAATGGGACGAGC
CGGCCTTCGAGCCCTACAAGGTCTTCGAGCGCGGCGGACGGCGCATCGGCGTGATCGGCCAGGCCTTCCCCTACATGCCA
ATCGCCAATCCGAAATGGATGTTTCCCGAATACAGCTTCGGCATCCGCGAGGAACGCATGCAGCAGGTCGTGGACGAGTT
GCGCGGCGAAGGCGTCGATCTGGTCGTGCTACTGTCGCATAACGGCTTCGACGTGGACAAGAAGATGGCCGGCCGCGTCA
AGGGCATCGACGTGATCCTGTCGGGCCATACCCATGACGCCGTGCCCGAGCCGATCCTGATCGGCGAGACGATCCTGATC
GCCACCGGCTCGAACGGCAAGTTCGTCAGCCGCGTCGATCTGGACCTGCGCGACGGCAAGATGATGGGCTTTCGCCACAA
GCTGATCCCGATCTTTTCGGACGTGATCGCGCCGGATGCCGAGATGGCCGCGCTGATCGACGCCGAGCGCGCGCCCTTCA
AGGCGCAGCTCGAGGAAAGGATCGGCACCACCGAAAGCCTGCTCTACCGGCGCGGCAACTTCAACGGCTCGTGGGACGAC
CTGATCTGCGACGCGGTGCGTTCCGAACGCGATGCGCAGATCGCGCTGTCGCCGGGCGTGCGCTGGGGCACCACGCTCTT
GCCCGGCGATGCGATCACCCGCGAGGACATCCACAACGTCACCTCGATGACCTATGGCGCGGTCTACCGCACCGAGATGA
CCGGCGAGATGCTCAAGACCATCCTGGAGGATGTCGCCGACAACATCTTCAACACCGATCCCTATTACCAGCAGGGCGGC
GACATGGTGCGCGTGGGCGGGCTGGCCTATGCGATCGACATCTCAAAGCCCATGGGCGAACGGATCGGCGCCATGGCGCT
GGCCGATACCGGCGAGGCCATCGAAGCCGCGAAATCCTACACGGTCGCCGGCTGGGCCTCGGTCAACGAAGGCACCGAAG
GCCCGCAAATCTGGGACGTGATCGAGACGCATATCCGCAAGATCGGCACCGTCCGGCTGCAACCCAACACCTCGGTCACC
GTGACCGGGGTCTGA

Upstream 100 bases:

>100_bases
GCGTCCGGCGGCCCGACCCCAACCTTCGAAAAGGGCATGTCCGACGCGCCTTGGCGCGGCGGGGAAGTCTTGCCCGAAAC
GCATGAGGAGTAACCGAGGA

Downstream 100 bases:

>100_bases
CATCACGAAGGAGCGTTCGCCCATGAAAGACGAACCCACCGGGAACGGCAAGGATGCCGCGGGCACCTCGCGCCGTGCCT
TCCTGCGCGCGGGCGCGGCG

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MITRREFFQAAMAASALVGASGFGTWSRVAAQQALTQDQLLQFDDFGNVTLIHVTDIHAQLKPIYFREPEINIGVGDAKG
QVPHVTGAAFQQMFGIAPGSADAYALSYPDFEALARGYGRMGGMDRVATVVKAIRAARPDSILLDGGDTWHGSMTSFLTK
GQDMVNVMNALGVDAMTSHWEWTYGTERVKEIVETQLKFPFLGANIFDAEWDEPAFEPYKVFERGGRRIGVIGQAFPYMP
IANPKWMFPEYSFGIREERMQQVVDELRGEGVDLVVLLSHNGFDVDKKMAGRVKGIDVILSGHTHDAVPEPILIGETILI
ATGSNGKFVSRVDLDLRDGKMMGFRHKLIPIFSDVIAPDAEMAALIDAERAPFKAQLEERIGTTESLLYRRGNFNGSWDD
LICDAVRSERDAQIALSPGVRWGTTLLPGDAITREDIHNVTSMTYGAVYRTEMTGEMLKTILEDVADNIFNTDPYYQQGG
DMVRVGGLAYAIDISKPMGERIGAMALADTGEAIEAAKSYTVAGWASVNEGTEGPQIWDVIETHIRKIGTVRLQPNTSVT
VTGV

Sequences:

>Translated_564_residues
MITRREFFQAAMAASALVGASGFGTWSRVAAQQALTQDQLLQFDDFGNVTLIHVTDIHAQLKPIYFREPEINIGVGDAKG
QVPHVTGAAFQQMFGIAPGSADAYALSYPDFEALARGYGRMGGMDRVATVVKAIRAARPDSILLDGGDTWHGSMTSFLTK
GQDMVNVMNALGVDAMTSHWEWTYGTERVKEIVETQLKFPFLGANIFDAEWDEPAFEPYKVFERGGRRIGVIGQAFPYMP
IANPKWMFPEYSFGIREERMQQVVDELRGEGVDLVVLLSHNGFDVDKKMAGRVKGIDVILSGHTHDAVPEPILIGETILI
ATGSNGKFVSRVDLDLRDGKMMGFRHKLIPIFSDVIAPDAEMAALIDAERAPFKAQLEERIGTTESLLYRRGNFNGSWDD
LICDAVRSERDAQIALSPGVRWGTTLLPGDAITREDIHNVTSMTYGAVYRTEMTGEMLKTILEDVADNIFNTDPYYQQGG
DMVRVGGLAYAIDISKPMGERIGAMALADTGEAIEAAKSYTVAGWASVNEGTEGPQIWDVIETHIRKIGTVRLQPNTSVT
VTGV
>Mature_564_residues
MITRREFFQAAMAASALVGASGFGTWSRVAAQQALTQDQLLQFDDFGNVTLIHVTDIHAQLKPIYFREPEINIGVGDAKG
QVPHVTGAAFQQMFGIAPGSADAYALSYPDFEALARGYGRMGGMDRVATVVKAIRAARPDSILLDGGDTWHGSMTSFLTK
GQDMVNVMNALGVDAMTSHWEWTYGTERVKEIVETQLKFPFLGANIFDAEWDEPAFEPYKVFERGGRRIGVIGQAFPYMP
IANPKWMFPEYSFGIREERMQQVVDELRGEGVDLVVLLSHNGFDVDKKMAGRVKGIDVILSGHTHDAVPEPILIGETILI
ATGSNGKFVSRVDLDLRDGKMMGFRHKLIPIFSDVIAPDAEMAALIDAERAPFKAQLEERIGTTESLLYRRGNFNGSWDD
LICDAVRSERDAQIALSPGVRWGTTLLPGDAITREDIHNVTSMTYGAVYRTEMTGEMLKTILEDVADNIFNTDPYYQQGG
DMVRVGGLAYAIDISKPMGERIGAMALADTGEAIEAAKSYTVAGWASVNEGTEGPQIWDVIETHIRKIGTVRLQPNTSVT
VTGV

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=428, Percent_Identity=27.1028037383178, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1790658, Length=215, Percent_Identity=28.8372093023256, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786687, Length=319, Percent_Identity=25.705329153605, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161076508, Length=478, Percent_Identity=24.2677824267782, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652512, Length=478, Percent_Identity=24.2677824267782, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19921980, Length=478, Percent_Identity=24.2677824267782, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28573524, Length=543, Percent_Identity=23.2044198895028, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221329836, Length=195, Percent_Identity=31.2820512820513, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641187, Length=195, Percent_Identity=31.2820512820513, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 61889; Mature: 61889

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRREFFQAAMAASALVGASGFGTWSRVAAQQALTQDQLLQFDDFGNVTLIHVTDIHAQ
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHEECCCCCEEEEEEEECCCC
LKPIYFREPEINIGVGDAKGQVPHVTGAAFQQMFGIAPGSADAYALSYPDFEALARGYGR
CCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHC
MGGMDRVATVVKAIRAARPDSILLDGGDTWHGSMTSFLTKGQDMVNVMNALGVDAMTSHW
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHCCC
EWTYGTERVKEIVETQLKFPFLGANIFDAEWDEPAFEPYKVFERGGRRIGVIGQAFPYMP
CCCCCHHHHHHHHHHHHCCCEECCEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCC
IANPKWMFPEYSFGIREERMQQVVDELRGEGVDLVVLLSHNGFDVDKKMAGRVKGIDVIL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCEEEEE
SGHTHDAVPEPILIGETILIATGSNGKFVSRVDLDLRDGKMMGFRHKLIPIFSDVIAPDA
ECCCCCCCCCCEEECCEEEEEECCCCCEEEEEECEECCCCCCCHHHHHHHHHHHHHCCCH
EMAALIDAERAPFKAQLEERIGTTESLLYRRGNFNGSWDDLICDAVRSERDAQIALSPGV
HHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
RWGTTLLPGDAITREDIHNVTSMTYGAVYRTEMTGEMLKTILEDVADNIFNTDPYYQQGG
CCCCEECCCCCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
DMVRVGGLAYAIDISKPMGERIGAMALADTGEAIEAAKSYTVAGWASVNEGTEGPQIWDV
CEEEECCEEEEEECCCCHHHHHCCEEECCCCHHHHHHHCEEEEEECCCCCCCCCCHHHHH
IETHIRKIGTVRLQPNTSVTVTGV
HHHHHHHHCEEEECCCCCEEEEEC
>Mature Secondary Structure
MITRREFFQAAMAASALVGASGFGTWSRVAAQQALTQDQLLQFDDFGNVTLIHVTDIHAQ
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHEECCCCCEEEEEEEECCCC
LKPIYFREPEINIGVGDAKGQVPHVTGAAFQQMFGIAPGSADAYALSYPDFEALARGYGR
CCCEEEECCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHC
MGGMDRVATVVKAIRAARPDSILLDGGDTWHGSMTSFLTKGQDMVNVMNALGVDAMTSHW
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHCCC
EWTYGTERVKEIVETQLKFPFLGANIFDAEWDEPAFEPYKVFERGGRRIGVIGQAFPYMP
CCCCCHHHHHHHHHHHHCCCEECCEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCC
IANPKWMFPEYSFGIREERMQQVVDELRGEGVDLVVLLSHNGFDVDKKMAGRVKGIDVIL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCEEEEE
SGHTHDAVPEPILIGETILIATGSNGKFVSRVDLDLRDGKMMGFRHKLIPIFSDVIAPDA
ECCCCCCCCCCEEECCEEEEEECCCCCEEEEEECEECCCCCCCHHHHHHHHHHHHHCCCH
EMAALIDAERAPFKAQLEERIGTTESLLYRRGNFNGSWDDLICDAVRSERDAQIALSPGV
HHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
RWGTTLLPGDAITREDIHNVTSMTYGAVYRTEMTGEMLKTILEDVADNIFNTDPYYQQGG
CCCCEECCCCCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
DMVRVGGLAYAIDISKPMGERIGAMALADTGEAIEAAKSYTVAGWASVNEGTEGPQIWDV
CEEEECCEEEEEECCCCHHHHHCCEEECCCCHHHHHHHCEEEEEECCCCCCCCCCHHHHH
IETHIRKIGTVRLQPNTSVTVTGV
HHHHHHHHCEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]