Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 119386736

GI number: 119386736

Start: 1177155

End: 1178417

Strand: Direct

Name: purD [H]

Synonym: Pden_4029

Alternate gene names: 119386736

Gene position: 1177155-1178417 (Clockwise)

Preceding gene: 119386733

Following gene: 119386737

Centisome position: 68.04

GC content: 70.94

Gene sequence:

>1263_bases
ATGAACATCCTGATCCTCGGCAGCGGCGGGCGCGAACATGCGCTGGCCTGGGCGATCCGGCAGAACCCGAAATGCGACCG
GCTGTTCGTCGCGCCCGGCAATGCCGGGATGGAACCCCTGGCGCGACTGGCGGATCTGGACATCCTGTCCGGCCCCGCCG
TGATCGGCTTTTGCGAGGAGAACGCCATCGACCTGGTGGTGATCGGCCCCGAGGCGCCGCTGGCCGCCGGCGTCGCCGAC
GAATTGCGCGCAGCGGGCATCCTGGCCTTCGGGCCGTCCAGGGCGGCGGCGATGCTGGAGGCATCGAAAAGCTTCACCAA
GGAGGTCTGCGACGCCTGCGGCGCCCCCACCGCCGCCTGGGCGCGTTTCGATGCGGCCGAACCGGCGCGGGCCTATATCC
GCGCGCAGGGCGCACCCATCGTCGTCAAGGCCGACGGGCTGGCCGCCGGCAAGGGCGTGACCGTCGCCATGACCGAGGCC
GAGGCCATCGCCGCAGTGGACGACGCTTTCGGCGGTGCCTTCGGCGAGGCCGGCGCCGAGGTGGTGATCGAGGAATTCAT
GGCCGGCGAGGAGGCGAGCTTCTTCGTCCTTTGCGACGGCAAGGACTGCCTGCCCATCGGCACGGCACAGGACCACAAGC
GCGCCTTTGACGGCGATACCGGGCCGAATACCGGCGGCATGGGGGCCTATTCCCCTGCCCCGGTGCTGACCCAGGCGATC
CAGGACCAGGTGATGGAGCAGATCGTGCGCCCCACCGTGGCCGAGATGGCGCGGCGCGGCACGCCGTTCCAGGGCGTGCT
CTATGCCGGGCTGATGATCGAACACGGCCGGGCGCGGCTGGTGGAATACAACGTCCGCTTCGGCGATCCGGAATGCCAGG
TGCTGATGATGCGGCTGGGCGCGCAGGCGCTGGACCTGATCCTGGCCTGCGCCGAGGGCCGGCTGGGCGACATGGCGGTC
AGTTGGGCCCAGGACCATGCCCTGACCGTGGTGCTGGCCGCGAAGGGTTATCCCGGCGCCTATGAGAAGGGCAGCGCGAT
CCGCGGGCTCGATGCGCTGCCCGAGGACAGCTTCCGCATGGTCTTCCATGCCGGGACGGCGCGGCGGGACGGGCAGATCG
TCGCCACCGGCGGGCGGGTTCTGGCGGCGACCGGGCGCGGCGCGACGCTGGCCGAGGCGCATGCGCGCGCCTATGCGCTG
GTGGATGCCATCGACTGGCCCGAGGGATTCTGCCGCCGCGACATCGGCTGGCGCGCGCTTTGA

Upstream 100 bases:

>100_bases
CGCGGGGGGCGGACGGGATCAAGGCCGGATTCTTGGGGATTGATCCGGGCCGGGCCAGTTCCTAGAAGTCGCCAGACATT
CGCAAGGGGGCCTTTTCCGC

Downstream 100 bases:

>100_bases
GTCGCCCCGCCGCCGGCACGGTCGCATGAGTATTTGAAGAACGAAGAAGTCCGGGGGGCGGTGCGGGGCGGCATCGCCCC
TGCGGATTTCGGGGCGGGAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNILILGSGGREHALAWAIRQNPKCDRLFVAPGNAGMEPLARLADLDILSGPAVIGFCEENAIDLVVIGPEAPLAAGVAD
ELRAAGILAFGPSRAAAMLEASKSFTKEVCDACGAPTAAWARFDAAEPARAYIRAQGAPIVVKADGLAAGKGVTVAMTEA
EAIAAVDDAFGGAFGEAGAEVVIEEFMAGEEASFFVLCDGKDCLPIGTAQDHKRAFDGDTGPNTGGMGAYSPAPVLTQAI
QDQVMEQIVRPTVAEMARRGTPFQGVLYAGLMIEHGRARLVEYNVRFGDPECQVLMMRLGAQALDLILACAEGRLGDMAV
SWAQDHALTVVLAAKGYPGAYEKGSAIRGLDALPEDSFRMVFHAGTARRDGQIVATGGRVLAATGRGATLAEAHARAYAL
VDAIDWPEGFCRRDIGWRAL

Sequences:

>Translated_420_residues
MNILILGSGGREHALAWAIRQNPKCDRLFVAPGNAGMEPLARLADLDILSGPAVIGFCEENAIDLVVIGPEAPLAAGVAD
ELRAAGILAFGPSRAAAMLEASKSFTKEVCDACGAPTAAWARFDAAEPARAYIRAQGAPIVVKADGLAAGKGVTVAMTEA
EAIAAVDDAFGGAFGEAGAEVVIEEFMAGEEASFFVLCDGKDCLPIGTAQDHKRAFDGDTGPNTGGMGAYSPAPVLTQAI
QDQVMEQIVRPTVAEMARRGTPFQGVLYAGLMIEHGRARLVEYNVRFGDPECQVLMMRLGAQALDLILACAEGRLGDMAV
SWAQDHALTVVLAAKGYPGAYEKGSAIRGLDALPEDSFRMVFHAGTARRDGQIVATGGRVLAATGRGATLAEAHARAYAL
VDAIDWPEGFCRRDIGWRAL
>Mature_420_residues
MNILILGSGGREHALAWAIRQNPKCDRLFVAPGNAGMEPLARLADLDILSGPAVIGFCEENAIDLVVIGPEAPLAAGVAD
ELRAAGILAFGPSRAAAMLEASKSFTKEVCDACGAPTAAWARFDAAEPARAYIRAQGAPIVVKADGLAAGKGVTVAMTEA
EAIAAVDDAFGGAFGEAGAEVVIEEFMAGEEASFFVLCDGKDCLPIGTAQDHKRAFDGDTGPNTGGMGAYSPAPVLTQAI
QDQVMEQIVRPTVAEMARRGTPFQGVLYAGLMIEHGRARLVEYNVRFGDPECQVLMMRLGAQALDLILACAEGRLGDMAV
SWAQDHALTVVLAAKGYPGAYEKGSAIRGLDALPEDSFRMVFHAGTARRDGQIVATGGRVLAATGRGATLAEAHARAYAL
VDAIDWPEGFCRRDIGWRAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=45.4117647058823, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=45.4117647058823, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=45.4117647058823, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=45.1536643026005, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=52.9411764705882, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=40.2843601895735, Blast_Score=288, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=40.990990990991, Blast_Score=296, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24582400, Length=413, Percent_Identity=41.6464891041162, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI62471619, Length=413, Percent_Identity=41.6464891041162, Blast_Score=285, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43913; Mature: 43913

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILILGSGGREHALAWAIRQNPKCDRLFVAPGNAGMEPLARLADLDILSGPAVIGFCEE
CEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCEECCCEEEEEECC
NAIDLVVIGPEAPLAAGVADELRAAGILAFGPSRAAAMLEASKSFTKEVCDACGAPTAAW
CCEEEEEECCCCCCCCCCHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
ARFDAAEPARAYIRAQGAPIVVKADGLAAGKGVTVAMTEAEAIAAVDDAFGGAFGEAGAE
HHCCCCCCHHHHEEECCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHH
VVIEEFMAGEEASFFVLCDGKDCLPIGTAQDHKRAFDGDTGPNTGGMGAYSPAPVLTQAI
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QDQVMEQIVRPTVAEMARRGTPFQGVLYAGLMIEHGRARLVEYNVRFGDPECQVLMMRLG
HHHHHHHHHCCHHHHHHHCCCCCHHHHEEEEEEECCCEEEEEEEEEECCCHHHHHHHHHC
AQALDLILACAEGRLGDMAVSWAQDHALTVVLAAKGYPGAYEKGSAIRGLDALPEDSFRM
HHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEE
VFHAGTARRDGQIVATGGRVLAATGRGATLAEAHARAYALVDAIDWPEGFCRRDIGWRAL
EEECCCCCCCCEEEEECCEEEEECCCCCCHHHHHHHHEEEEECCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MNILILGSGGREHALAWAIRQNPKCDRLFVAPGNAGMEPLARLADLDILSGPAVIGFCEE
CEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCEECCCEEEEEECC
NAIDLVVIGPEAPLAAGVADELRAAGILAFGPSRAAAMLEASKSFTKEVCDACGAPTAAW
CCEEEEEECCCCCCCCCCHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
ARFDAAEPARAYIRAQGAPIVVKADGLAAGKGVTVAMTEAEAIAAVDDAFGGAFGEAGAE
HHCCCCCCHHHHEEECCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHH
VVIEEFMAGEEASFFVLCDGKDCLPIGTAQDHKRAFDGDTGPNTGGMGAYSPAPVLTQAI
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QDQVMEQIVRPTVAEMARRGTPFQGVLYAGLMIEHGRARLVEYNVRFGDPECQVLMMRLG
HHHHHHHHHCCHHHHHHHCCCCCHHHHEEEEEEECCCEEEEEEEEEECCCHHHHHHHHHC
AQALDLILACAEGRLGDMAVSWAQDHALTVVLAAKGYPGAYEKGSAIRGLDALPEDSFRM
HHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEE
VFHAGTARRDGQIVATGGRVLAATGRGATLAEAHARAYALVDAIDWPEGFCRRDIGWRAL
EEECCCCCCCCEEEEECCEEEEECCCCCCHHHHHHHHEEEEECCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]