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The map label for this gene is alkA [C]

Identifier: 119386652

GI number: 119386652

Start: 1082244

End: 1082867

Strand: Direct

Name: alkA [C]

Synonym: Pden_3945

Alternate gene names: 119386652

Gene position: 1082244-1082867 (Clockwise)

Preceding gene: 119386651

Following gene: 119386653

Centisome position: 62.55

GC content: 73.72

Gene sequence:

>624_bases
ATGCGCGAAATCCGCGCCCCGGCCGATCTGGAGGAGGGCGCGGCGCATCTGATGCGGGTCTGTCCGGTCTGGGCGCGCGA
GTTGCCGGCGCTGATGCCGCTGCCGCTGCGGCGCTGGCCCGAAGGGTTCCCGGCAATCCGCGATGCGGTGGTTTCGCAGC
AGATCTCGACCCAGGCTGCCAATGCCATCGCCGCGCGCATGGCTGCGGCGGGGCTGGCCGATGAGGCGGGAATCGCCGCC
GCGGATGACGAGGCGCTGCGCGCCGCCGGCCTGTCGCGGCCCAAGATCCGCTATCTGCGCGGCATTGCCGGGGCAGGGGT
GGACTGGGCGGGCTTGCGCGACCTGCCCGACGAGGCGGTGATCGCCCGGCTGACCGCCTTGCCGGGAATCGGGCGCTGGA
CCGCCGACATCTATCTGAAATTCGCGCTTGGCCGGGCGGATGCCTTTGCTGCGGGTGATCTGGCGCTGGCCGAGGCGGCC
CGGCTGCTCCATGGCCTGCCGGAACGCCCCCGTCCGGCCGCCCTGACCGAGCTGGCCGAGCCGTGGCGGCCCTGGCGGGC
GGTTGCGGCCCGGGCGCTCTGGGCCTATTACCGGGCCGCAAAAGGGCGCGAGGGCGTCCGCTGA

Upstream 100 bases:

>100_bases
ACGGGGCGGCGGCGGAACAGGCGCTGCGCCATCACATCTCGATGGCCTTCGAGACCCGGCTGAAGGAAGAAGCGCGGCTG
GCCGACGAGGAAGAGGATTG

Downstream 100 bases:

>100_bases
GGGGGACGAGATGCTGCGCGAGTTGAAATCCGAACGCAAGGGGCCGCGCCAGGCCGATTCCGTGGTGGTCTTTCTGCACG
GCTACGGCGCCGACGGGGCC

Product: HhH-GPD family protein

Products: NA

Alternate protein names: DNA-3-Methyladenine Glycosylase II; HhH-GPD Family Protein; Base Excision DNA Repair Protein HhH-GPD Family; DNA-Glycosylase Family Protein

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MREIRAPADLEEGAAHLMRVCPVWARELPALMPLPLRRWPEGFPAIRDAVVSQQISTQAANAIAARMAAAGLADEAGIAA
ADDEALRAAGLSRPKIRYLRGIAGAGVDWAGLRDLPDEAVIARLTALPGIGRWTADIYLKFALGRADAFAAGDLALAEAA
RLLHGLPERPRPAALTELAEPWRPWRAVAARALWAYYRAAKGREGVR

Sequences:

>Translated_207_residues
MREIRAPADLEEGAAHLMRVCPVWARELPALMPLPLRRWPEGFPAIRDAVVSQQISTQAANAIAARMAAAGLADEAGIAA
ADDEALRAAGLSRPKIRYLRGIAGAGVDWAGLRDLPDEAVIARLTALPGIGRWTADIYLKFALGRADAFAAGDLALAEAA
RLLHGLPERPRPAALTELAEPWRPWRAVAARALWAYYRAAKGREGVR
>Mature_207_residues
MREIRAPADLEEGAAHLMRVCPVWARELPALMPLPLRRWPEGFPAIRDAVVSQQISTQAANAIAARMAAAGLADEAGIAA
ADDEALRAAGLSRPKIRYLRGIAGAGVDWAGLRDLPDEAVIARLTALPGIGRWTADIYLKFALGRADAFAAGDLALAEAA
RLLHGLPERPRPAALTELAEPWRPWRAVAARALWAYYRAAKGREGVR

Specific function: Hydrolysis Of The Deoxyribose N-Glycosidic Bond To Excise 3- Methyladenine, 3- Methylguanine, 7- Methylguanine, O2- Methylthymine, And O2 -Methylcytosine From The Damaged DNA Polymer Formed By Alkylation Lesions. [C]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.2.21

Molecular weight: Translated: 22280; Mature: 22280

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIRAPADLEEGAAHLMRVCPVWARELPALMPLPLRRWPEGFPAIRDAVVSQQISTQAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH
NAIAARMAAAGLADEAGIAAADDEALRAAGLSRPKIRYLRGIAGAGVDWAGLRDLPDEAV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
IARLTALPGIGRWTADIYLKFALGRADAFAAGDLALAEAARLLHGLPERPRPAALTELAE
HHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
PWRPWRAVAARALWAYYRAAKGREGVR
CCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MREIRAPADLEEGAAHLMRVCPVWARELPALMPLPLRRWPEGFPAIRDAVVSQQISTQAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHH
NAIAARMAAAGLADEAGIAAADDEALRAAGLSRPKIRYLRGIAGAGVDWAGLRDLPDEAV
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
IARLTALPGIGRWTADIYLKFALGRADAFAAGDLALAEAARLLHGLPERPRPAALTELAE
HHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
PWRPWRAVAARALWAYYRAAKGREGVR
CCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Spermidine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0000092 {3-methyladenine} 0.000011 {7-methylguanine} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of N-glycosyl compounds [C]

Inhibitor: DNA with a purinic sites; Double-stranded DNA; NaCl; NEM; Pyrrolidine-containingoligo nucleotide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA