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The map label for this gene is purN [H]

Identifier: 119386634

GI number: 119386634

Start: 1063681

End: 1064277

Strand: Direct

Name: purN [H]

Synonym: Pden_3927

Alternate gene names: 119386634

Gene position: 1063681-1064277 (Clockwise)

Preceding gene: 119386633

Following gene: 119386641

Centisome position: 61.48

GC content: 70.69

Gene sequence:

>597_bases
GTGAAACGCGTCGCCATCCTGATCTCGGGCGGCGGCTCGAACATGGTCAAGCTGGTCGAATCCATGACCGGCACGCATCC
CGCGCGGCCGGTCGTCGTCGGCTCGAACGATCCGCAGGCAGCGGGCCTTGCCCGGGCGCAAGCCATGGGCGTGCCGAGCT
TCGCCATCGACCACCGGGCCTATCCCGGCGACCGCGCGGGCTTCGAGGCAGCGCTGCTGGAACCCCTGCTGGCCGCGCAG
CCCGACATCCTGTGCCTTGCCGGCTTCATGCGTATCCTGACCCCGGACTTCGTGCAGCGGTTCGAGGGCCGGATGCTGAA
CATCCACCCCTCGCTCCTGCCGAAATATCCGGGCTTGCATACGCATCAGCGCGCCATCGACGCCGGCGATGCCGAGGCCG
GGGCCAGCGTGCACCTGGTCACGCCGGAACTCGACGCCGGCCCGATCCTCGGCCAGGCCCGCGTGCCGGTCCTGCCCGGC
GATACGGCCGAGACGCTGGCCGCCCGCGTGCTGACCCAGGAACACCGGCTCTATCCCCAGGTGCTGCGCCGTTTCGCGCA
GGGCGACCTGACGCCGATCACCCTCACCGCGGCGTGA

Upstream 100 bases:

>100_bases
CCGCCGCAATCGAGGCGCTGCTGAAGGCCGAGGGCGAGACCGTGTTCCGCCTCGGCCAGGTCAGCCCCGGCGCAGGCGTG
GGCTACAGCGGCCGGCTGCG

Downstream 100 bases:

>100_bases
AAAAAGGCGCGGACATGCGCCTTTCTCCGTTTTCCAAATACTCTGGGGGTGCGGGGGCAAAGCCCCCGCGACTTCGCCTG
GCTATTTCTCGGGCACCAGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKRVAILISGGGSNMVKLVESMTGTHPARPVVVGSNDPQAAGLARAQAMGVPSFAIDHRAYPGDRAGFEAALLEPLLAAQ
PDILCLAGFMRILTPDFVQRFEGRMLNIHPSLLPKYPGLHTHQRAIDAGDAEAGASVHLVTPELDAGPILGQARVPVLPG
DTAETLAARVLTQEHRLYPQVLRRFAQGDLTPITLTAA

Sequences:

>Translated_198_residues
MKRVAILISGGGSNMVKLVESMTGTHPARPVVVGSNDPQAAGLARAQAMGVPSFAIDHRAYPGDRAGFEAALLEPLLAAQ
PDILCLAGFMRILTPDFVQRFEGRMLNIHPSLLPKYPGLHTHQRAIDAGDAEAGASVHLVTPELDAGPILGQARVPVLPG
DTAETLAARVLTQEHRLYPQVLRRFAQGDLTPITLTAA
>Mature_198_residues
MKRVAILISGGGSNMVKLVESMTGTHPARPVVVGSNDPQAAGLARAQAMGVPSFAIDHRAYPGDRAGFEAALLEPLLAAQ
PDILCLAGFMRILTPDFVQRFEGRMLNIHPSLLPKYPGLHTHQRAIDAGDAEAGASVHLVTPELDAGPILGQARVPVLPG
DTAETLAARVLTQEHRLYPQVLRRFAQGDLTPITLTAA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=43.8502673796791, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=43.8502673796791, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=43.8502673796791, Blast_Score=158, Evalue=3e-39,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=46.8421052631579, Blast_Score=175, Evalue=1e-45,
Organism=Escherichia coli, GI1787483, Length=135, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=40.3314917127072, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=45.5497382198953, Blast_Score=166, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21004; Mature: 21004

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVAILISGGGSNMVKLVESMTGTHPARPVVVGSNDPQAAGLARAQAMGVPSFAIDHRA
CCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHCCCC
YPGDRAGFEAALLEPLLAAQPDILCLAGFMRILTPDFVQRFEGRMLNIHPSLLPKYPGLH
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCCEEEECHHHCCCCCCCC
THQRAIDAGDAEAGASVHLVTPELDAGPILGQARVPVLPGDTAETLAARVLTQEHRLYPQ
CHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLRRFAQGDLTPITLTAA
HHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MKRVAILISGGGSNMVKLVESMTGTHPARPVVVGSNDPQAAGLARAQAMGVPSFAIDHRA
CCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCHHHCCCC
YPGDRAGFEAALLEPLLAAQPDILCLAGFMRILTPDFVQRFEGRMLNIHPSLLPKYPGLH
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCCEEEECHHHCCCCCCCC
THQRAIDAGDAEAGASVHLVTPELDAGPILGQARVPVLPGDTAETLAARVLTQEHRLYPQ
CHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLRRFAQGDLTPITLTAA
HHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]