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The map label for this gene is pckA

Identifier: 119385577

GI number: 119385577

Start: 17197

End: 18795

Strand: Direct

Name: pckA

Synonym: Pden_2852

Alternate gene names: 119385577

Gene position: 17197-18795 (Clockwise)

Preceding gene: 119385570

Following gene: 119385587

Centisome position: 0.99

GC content: 65.1

Gene sequence:

>1599_bases
ATGACCGAACCGCGCGTCAATCCGAATTGCCGTCTCGAAGACCAGGGAATCACGGGCCTCGGCAATGTCCACTACAACCT
GCTAGAACCGGCGCTAATCGACGCTGCCATCCAGCGCGGCGAAGGCAAGCTGGGCTTGGGCGGCACCTTCCTGGTCTCGA
CCGGCGCCCATACCGGCCGCTCGCCCAAGGACAAGCATGTGGTGCGCACGCCCGCGGTGGAAGACAGCATCTGGTGGGAA
AACAACCGCCCGATGGAGCCCGAGGCCTTCGACCGCCTGCATGCCGACATGCTGGAGCACATGAAAGGCAAGGACTATTT
CGTCCAGGACCTCTACGGCGGCGCGGATCCTGCCCTGCGCCTCGACGTGCGGGTCGTGACCGAACTGGCCTGGCACGGGC
TCTTCATCCGCAATCTGCTGCGCCGTCCCGAAGCGTCCGAACTGGCCGGTTTCCTGCCGGAATTCACCATCATCAACTGC
CCCTCGTTCAAGGCCGACCCGGCACGCCACGGCTGCCGCTCGGAGACGGTGATCGCGCTCAACTTCGACAAGAAGCTGAT
CCTGATCGGCAATACCGCCTATGCGGGCGAGAACAAGAAGTCGGTCTTCACGCTCCTGAACTACATCCTGCCGGAAAAGG
GCGTGATGCCGATGCATTGCTCGGCCAACCACGCGCTCGGCAACCCGAACGACAGCGCCATCTTCTTCGGCCTGTCCGGC
ACCGGCAAGACCACGCTCTCGGCCGATCCCTCGCGCACGCTGATCGGCGATGACGAGCACGGCTGGTCCGACAACGGCAT
CTTCAACTTCGAGGGCGGCTGCTACGCCAAGACCATCAATCTTTCGGCCGAGGCCGAACCCGAGATCTATGCCACCTGCT
TCAAGTTCGGCACCGTGATCGAGAACATGGTCTACGACCCCGACACGCTGGAACTGGACTTCGAGGATAACTCGCTGACC
GACAACATGCGCTGCGCCTATCCGCTGGAGCAGATCTCGAACGCGTCGGAAACCTCGCTGGGCGGCCAGCCCCGGAACGT
GATCATGCTGACCTGCGACGCCTATGGCGTGCTGCCGCCCATCGCGCGGCTGACCCCGGCGCAGGCGATGTATCACTTCC
TGTCGGGCTTCACCTCCAAGACGCCGGGCACAGAGGTGGGCGTGACCGAGCCGACGCCGACCTTCTCGACCTGCTTCGGC
GCCCCCTTCATGCCGCGCCGCCCCGAGGTCTATGGCAAGCTCCTGCAGGAAAAGATCGCCAAGACCGGCGCGGCCTGCTG
GCTGGTCAACACCGGCTGGACCGGCGGCGCCTTCGGCACCGGCAAGCGCATGCCGATCAAGGCGACCCGTGCCCTGCTGA
CCGCGGCGCTGGACGGCTCGCTGAACGACGCCCAGTTCCGCAAGGACCCGAATTTCGGCTTCGAGGTTCCGGTCTCGGTC
CCCGGCGTCGAGGACAAGCTGCTCGACCCGCGCCAGACCTGGACGGACGGCGCCGCCTACGACGCCCAGGCCCGCAAGCT
GGTCGGCATGTTCAGCGACAACTTCGCGCAATATGCCGACAAGATCGACGACGACGTCCGCGCCGCCGCCATCGGCTGA

Upstream 100 bases:

>100_bases
AACCCTGACGATAAGTGCGCTTTCGCCCGGGAAATGCCGTGTTATAGGACGCGCAACGGCCGGTTGCCGGCCCGGAATTT
CTAGCAGGAGCACAGGTGCC

Downstream 100 bases:

>100_bases
ACCGGACCGAAAATCTCAAACGCAAAGGGCGGTCCCAAGGGCCGCTCTTTTGCTTCGCACCAAAGCCCAGCCCACGCGGA
ACGATCCCGCCGCCCAGGCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTEPRVNPNCRLEDQGITGLGNVHYNLLEPALIDAAIQRGEGKLGLGGTFLVSTGAHTGRSPKDKHVVRTPAVEDSIWWE
NNRPMEPEAFDRLHADMLEHMKGKDYFVQDLYGGADPALRLDVRVVTELAWHGLFIRNLLRRPEASELAGFLPEFTIINC
PSFKADPARHGCRSETVIALNFDKKLILIGNTAYAGENKKSVFTLLNYILPEKGVMPMHCSANHALGNPNDSAIFFGLSG
TGKTTLSADPSRTLIGDDEHGWSDNGIFNFEGGCYAKTINLSAEAEPEIYATCFKFGTVIENMVYDPDTLELDFEDNSLT
DNMRCAYPLEQISNASETSLGGQPRNVIMLTCDAYGVLPPIARLTPAQAMYHFLSGFTSKTPGTEVGVTEPTPTFSTCFG
APFMPRRPEVYGKLLQEKIAKTGAACWLVNTGWTGGAFGTGKRMPIKATRALLTAALDGSLNDAQFRKDPNFGFEVPVSV
PGVEDKLLDPRQTWTDGAAYDAQARKLVGMFSDNFAQYADKIDDDVRAAAIG

Sequences:

>Translated_532_residues
MTEPRVNPNCRLEDQGITGLGNVHYNLLEPALIDAAIQRGEGKLGLGGTFLVSTGAHTGRSPKDKHVVRTPAVEDSIWWE
NNRPMEPEAFDRLHADMLEHMKGKDYFVQDLYGGADPALRLDVRVVTELAWHGLFIRNLLRRPEASELAGFLPEFTIINC
PSFKADPARHGCRSETVIALNFDKKLILIGNTAYAGENKKSVFTLLNYILPEKGVMPMHCSANHALGNPNDSAIFFGLSG
TGKTTLSADPSRTLIGDDEHGWSDNGIFNFEGGCYAKTINLSAEAEPEIYATCFKFGTVIENMVYDPDTLELDFEDNSLT
DNMRCAYPLEQISNASETSLGGQPRNVIMLTCDAYGVLPPIARLTPAQAMYHFLSGFTSKTPGTEVGVTEPTPTFSTCFG
APFMPRRPEVYGKLLQEKIAKTGAACWLVNTGWTGGAFGTGKRMPIKATRALLTAALDGSLNDAQFRKDPNFGFEVPVSV
PGVEDKLLDPRQTWTDGAAYDAQARKLVGMFSDNFAQYADKIDDDVRAAAIG
>Mature_531_residues
TEPRVNPNCRLEDQGITGLGNVHYNLLEPALIDAAIQRGEGKLGLGGTFLVSTGAHTGRSPKDKHVVRTPAVEDSIWWEN
NRPMEPEAFDRLHADMLEHMKGKDYFVQDLYGGADPALRLDVRVVTELAWHGLFIRNLLRRPEASELAGFLPEFTIINCP
SFKADPARHGCRSETVIALNFDKKLILIGNTAYAGENKKSVFTLLNYILPEKGVMPMHCSANHALGNPNDSAIFFGLSGT
GKTTLSADPSRTLIGDDEHGWSDNGIFNFEGGCYAKTINLSAEAEPEIYATCFKFGTVIENMVYDPDTLELDFEDNSLTD
NMRCAYPLEQISNASETSLGGQPRNVIMLTCDAYGVLPPIARLTPAQAMYHFLSGFTSKTPGTEVGVTEPTPTFSTCFGA
PFMPRRPEVYGKLLQEKIAKTGAACWLVNTGWTGGAFGTGKRMPIKATRALLTAALDGSLNDAQFRKDPNFGFEVPVSVP
GVEDKLLDPRQTWTDGAAYDAQARKLVGMFSDNFAQYADKIDDDVRAAAIG

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=537, Percent_Identity=46.1824953445065, Blast_Score=469, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322950, Length=535, Percent_Identity=43.7383177570093, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_PARDP (A1B5Z2)

Other databases:

- EMBL:   CP000490
- RefSeq:   YP_916632.1
- ProteinModelPortal:   A1B5Z2
- SMR:   A1B5Z2
- STRING:   A1B5Z2
- GeneID:   4581439
- GenomeReviews:   CP000490_GR
- KEGG:   pde:Pden_2852
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   A1B5Z2
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58133; Mature: 58002

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRVNPNCRLEDQGITGLGNVHYNLLEPALIDAAIQRGEGKLGLGGTFLVSTGAHTGR
CCCCCCCCCCEECCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
SPKDKHVVRTPAVEDSIWWENNRPMEPEAFDRLHADMLEHMKGKDYFVQDLYGGADPALR
CCCCCCEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCEE
LDVRVVTELAWHGLFIRNLLRRPEASELAGFLPEFTIINCPSFKADPARHGCRSETVIAL
EHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCEEEEE
NFDKKLILIGNTAYAGENKKSVFTLLNYILPEKGVMPMHCSANHALGNPNDSAIFFGLSG
ECCCEEEEEECCEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEECC
TGKTTLSADPSRTLIGDDEHGWSDNGIFNFEGGCYAKTINLSAEAEPEIYATCFKFGTVI
CCCEEECCCCCCCEECCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
ENMVYDPDTLELDFEDNSLTDNMRCAYPLEQISNASETSLGGQPRNVIMLTCDAYGVLPP
HHHCCCCCCEEEEECCCCCCCCCEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCC
IARLTPAQAMYHFLSGFTSKTPGTEVGVTEPTPTFSTCFGAPFMPRRPEVYGKLLQEKIA
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
KTGAACWLVNTGWTGGAFGTGKRMPIKATRALLTAALDGSLNDAQFRKDPNFGFEVPVSV
HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEECEEC
PGVEDKLLDPRQTWTDGAAYDAQARKLVGMFSDNFAQYADKIDDDVRAAAIG
CCCHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure 
TEPRVNPNCRLEDQGITGLGNVHYNLLEPALIDAAIQRGEGKLGLGGTFLVSTGAHTGR
CCCCCCCCCEECCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
SPKDKHVVRTPAVEDSIWWENNRPMEPEAFDRLHADMLEHMKGKDYFVQDLYGGADPALR
CCCCCCEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCEE
LDVRVVTELAWHGLFIRNLLRRPEASELAGFLPEFTIINCPSFKADPARHGCRSETVIAL
EHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCEEEEE
NFDKKLILIGNTAYAGENKKSVFTLLNYILPEKGVMPMHCSANHALGNPNDSAIFFGLSG
ECCCEEEEEECCEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEEECC
TGKTTLSADPSRTLIGDDEHGWSDNGIFNFEGGCYAKTINLSAEAEPEIYATCFKFGTVI
CCCEEECCCCCCCEECCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
ENMVYDPDTLELDFEDNSLTDNMRCAYPLEQISNASETSLGGQPRNVIMLTCDAYGVLPP
HHHCCCCCCEEEEECCCCCCCCCEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCC
IARLTPAQAMYHFLSGFTSKTPGTEVGVTEPTPTFSTCFGAPFMPRRPEVYGKLLQEKIA
HHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
KTGAACWLVNTGWTGGAFGTGKRMPIKATRALLTAALDGSLNDAQFRKDPNFGFEVPVSV
HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEECEEC
PGVEDKLLDPRQTWTDGAAYDAQARKLVGMFSDNFAQYADKIDDDVRAAAIG
CCCHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA