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The map label for this gene is 119385570

Identifier: 119385570

GI number: 119385570

Start: 12258

End: 13637

Strand: Direct

Name: 119385570

Synonym: Pden_2845

Alternate gene names: NA

Gene position: 12258-13637 (Clockwise)

Preceding gene: 119385569

Following gene: 119385577

Centisome position: 0.71

GC content: 65.94

Gene sequence:

>1380_bases
ATGTCCATTCCCCAACAGGGCGGCGGCCCGATCGAACGTCCCGAACAACTGGCCGAATATCTCGCCTCGGGTGAAAAACC
GCGCGAGAGCTGGCGGATCGGCACCGAGCACGAGAAATTCGGCTATCGCCATGCCGACCTGATGCCCCTGCCCTATGAGG
CGCCCGCCGGCCAGCCCTCGGTCAAGGCCATGCTGGAGGGGTTGCAGGCCCGTTTCGGCTGGACCCCGGTGCTGGAGGCC
GGCCATATCATCGGGCTGGAACGCGACGGCGCCAATGTCAGCCTGGAGCCCGGCGGACAGCTAGAACTCTCCGGCGCGCC
GCTGGAGACCATCCACCAGACCTGCGACGAGGTGAACAGCCATCTGGCCGAGGTGAAGGCCGTGGCGGACGATCTTGGCG
CGGGCTTCATCGGCCTTGGCGCGGCGCCGATCTGGACGCAGGACCAGATGCCGATGATGCCGAAAGGCCGCTATCGGCTG
ATGACCGACTACATGGGTCGGGTCGGCACGCTGGGCACGCAGATGATGTATCGCACCTGCACGGTGCAGGTGAACCTGGA
CTTCGCCAGCGAAGCCGACATGGTGCAGAAGCTGCGCGTGGCGCTGGCCCTGCAGCCGGTGGCGAATGCGCTGTTCGCCA
ATTCGCCCTTCCTCGACGGCAAGCCGAACGGCTGGAAAAGCTGGCGGGCGCATATCTGGCAGAACCTCGACGCGGCCCGC
ACCGGCATGCTGCCCTTTGCCTTCGAGGACGGCTTCGGCTACGAGCGTTGGGTCGATTACGTCCTCGATGTGCCGATGTA
TTTCGTCTATCGCGACGGCAAGTATATCGACGCGTTGGGACAAAGCTTCCGCGATTTCCTGAACGGCCGCTTGCCGGCGC
TGCCGGGCGAAATCCCGACCCTGTCGGATTGGGCCGACCACCTGACCACGGTTTTCCCCGAGGCGCGGGTCAAGAAATTC
ATCGAGATGCGCGGTGCGGACGGCGGTCCCTGGCGGCGGCTCTGCGCCCTGCCGGCGCTGTGGGTCGGGCTGCTTTACGA
CCAGGCGGCACAGGATGCGGCCTGGGATCTGGTCAAGGGCTGGGATGCCGAGACGCGCGAGTCCTGGCGGCGCGGTGCCG
GGCTCAAGGCGCTGGATGCCGAGGTGAACGGCACGAAGATGCGCGACCTGGCGCGCGAGGTGCTGGCGATTTCGGAAGCC
GGGCTCAAGGCGCGTGCCCGCACCGGATCGGGCGGGCTGGTGCCGGACGAGACGCATTTCCTGAACACGCTGAAGGAAAG
CGTCGAGACCGGCAAGGTTCCCGCCGACGAGTTGCTGGGGAAATACCACGGCGAATGGGCGGGCGATCTCAGCCGCATCT
ACGCCGAATATTCCTATTGA

Upstream 100 bases:

>100_bases
CCGACGATCCAGACCGTTTGGACGAGGCCGGCGCGCGCCCGCTTGACTTGAGCCGCGCGCCTTGCCACCTGTGACGGGCA
AGGCAAATGAAAGGCCACCG

Downstream 100 bases:

>100_bases
CCAAGGGCCGGGTAATCCCCGGCCCCTTTCGCATCCGGCCTGCGTCGCAAATTCAGGCTTGCGGCGCCGACGGTTCGTTG
CGGTTTTGCATGAACTGGTC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSIPQQGGGPIERPEQLAEYLASGEKPRESWRIGTEHEKFGYRHADLMPLPYEAPAGQPSVKAMLEGLQARFGWTPVLEA
GHIIGLERDGANVSLEPGGQLELSGAPLETIHQTCDEVNSHLAEVKAVADDLGAGFIGLGAAPIWTQDQMPMMPKGRYRL
MTDYMGRVGTLGTQMMYRTCTVQVNLDFASEADMVQKLRVALALQPVANALFANSPFLDGKPNGWKSWRAHIWQNLDAAR
TGMLPFAFEDGFGYERWVDYVLDVPMYFVYRDGKYIDALGQSFRDFLNGRLPALPGEIPTLSDWADHLTTVFPEARVKKF
IEMRGADGGPWRRLCALPALWVGLLYDQAAQDAAWDLVKGWDAETRESWRRGAGLKALDAEVNGTKMRDLAREVLAISEA
GLKARARTGSGGLVPDETHFLNTLKESVETGKVPADELLGKYHGEWAGDLSRIYAEYSY

Sequences:

>Translated_459_residues
MSIPQQGGGPIERPEQLAEYLASGEKPRESWRIGTEHEKFGYRHADLMPLPYEAPAGQPSVKAMLEGLQARFGWTPVLEA
GHIIGLERDGANVSLEPGGQLELSGAPLETIHQTCDEVNSHLAEVKAVADDLGAGFIGLGAAPIWTQDQMPMMPKGRYRL
MTDYMGRVGTLGTQMMYRTCTVQVNLDFASEADMVQKLRVALALQPVANALFANSPFLDGKPNGWKSWRAHIWQNLDAAR
TGMLPFAFEDGFGYERWVDYVLDVPMYFVYRDGKYIDALGQSFRDFLNGRLPALPGEIPTLSDWADHLTTVFPEARVKKF
IEMRGADGGPWRRLCALPALWVGLLYDQAAQDAAWDLVKGWDAETRESWRRGAGLKALDAEVNGTKMRDLAREVLAISEA
GLKARARTGSGGLVPDETHFLNTLKESVETGKVPADELLGKYHGEWAGDLSRIYAEYSY
>Mature_458_residues
SIPQQGGGPIERPEQLAEYLASGEKPRESWRIGTEHEKFGYRHADLMPLPYEAPAGQPSVKAMLEGLQARFGWTPVLEAG
HIIGLERDGANVSLEPGGQLELSGAPLETIHQTCDEVNSHLAEVKAVADDLGAGFIGLGAAPIWTQDQMPMMPKGRYRLM
TDYMGRVGTLGTQMMYRTCTVQVNLDFASEADMVQKLRVALALQPVANALFANSPFLDGKPNGWKSWRAHIWQNLDAART
GMLPFAFEDGFGYERWVDYVLDVPMYFVYRDGKYIDALGQSFRDFLNGRLPALPGEIPTLSDWADHLTTVFPEARVKKFI
EMRGADGGPWRRLCALPALWVGLLYDQAAQDAAWDLVKGWDAETRESWRRGAGLKALDAEVNGTKMRDLAREVLAISEAG
LKARARTGSGGLVPDETHFLNTLKESVETGKVPADELLGKYHGEWAGDLSRIYAEYSY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 50821; Mature: 50690

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPQQGGGPIERPEQLAEYLASGEKPRESWRIGTEHEKFGYRHADLMPLPYEAPAGQPS
CCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHH
VKAMLEGLQARFGWTPVLEAGHIIGLERDGANVSLEPGGQLELSGAPLETIHQTCDEVNS
HHHHHHHHHHHCCCCHHHCCCCEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHH
HLAEVKAVADDLGAGFIGLGAAPIWTQDQMPMMPKGRYRLMTDYMGRVGTLGTQMMYRTC
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
TVQVNLDFASEADMVQKLRVALALQPVANALFANSPFLDGKPNGWKSWRAHIWQNLDAAR
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHH
TGMLPFAFEDGFGYERWVDYVLDVPMYFVYRDGKYIDALGQSFRDFLNGRLPALPGEIPT
HCCCCEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LSDWADHLTTVFPEARVKKFIEMRGADGGPWRRLCALPALWVGLLYDQAAQDAAWDLVKG
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WDAETRESWRRGAGLKALDAEVNGTKMRDLAREVLAISEAGLKARARTGSGGLVPDETHF
CCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHH
LNTLKESVETGKVPADELLGKYHGEWAGDLSRIYAEYSY
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPQQGGGPIERPEQLAEYLASGEKPRESWRIGTEHEKFGYRHADLMPLPYEAPAGQPS
CCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHH
VKAMLEGLQARFGWTPVLEAGHIIGLERDGANVSLEPGGQLELSGAPLETIHQTCDEVNS
HHHHHHHHHHHCCCCHHHCCCCEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHH
HLAEVKAVADDLGAGFIGLGAAPIWTQDQMPMMPKGRYRLMTDYMGRVGTLGTQMMYRTC
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
TVQVNLDFASEADMVQKLRVALALQPVANALFANSPFLDGKPNGWKSWRAHIWQNLDAAR
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHH
TGMLPFAFEDGFGYERWVDYVLDVPMYFVYRDGKYIDALGQSFRDFLNGRLPALPGEIPT
HCCCCEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LSDWADHLTTVFPEARVKKFIEMRGADGGPWRRLCALPALWVGLLYDQAAQDAAWDLVKG
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WDAETRESWRRGAGLKALDAEVNGTKMRDLAREVLAISEAGLKARARTGSGGLVPDETHF
CCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHH
LNTLKESVETGKVPADELLGKYHGEWAGDLSRIYAEYSY
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA