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The map label for this gene is amt [H]

Identifier: 119358436

GI number: 119358436

Start: 3064661

End: 3065992

Strand: Reverse

Name: amt [H]

Synonym: Cpha266_2672

Alternate gene names: 119358436

Gene position: 3065992-3064661 (Counterclockwise)

Preceding gene: 119358438

Following gene: 119358435

Centisome position: 97.83

GC content: 53.83

Gene sequence:

>1332_bases
ATGATGAAAAAAATCTCTGGTTCATTGCTGTTTCTCCTGGCAGTAATGATGTTCAGCGGAACAGTACATGCTGCTGACGA
GGTGGTAACCGATACCGGGACAACCTCATGGATGCTGACTTCGACAGCCCTTGTACTGCTTATGGTTCCGGGACTCGCCA
TGTTCTATGGCGGTCTTGTCCGCACGAAAAACGTTATCGGAACCATGATGCACAGCTTTGCAGCAATGGTAATTATCGGG
GTGCTCTGGCCTCTGGTAGGTTACTCGCTCAGTTTCGGCCCCAGCATTCTCGGCGGTCTGGCAGGGTGGGATAACAAATT
CTTCATGCTGCAGGGCATTGATGAGTCGATCATGTCGACGCAGATACCCGAATATGTCTTTGCGATGTTTCAGGGCAAGT
TCGCCATTATCACTCCTGCGCTTATTGCCGGAGCATTTGCCGAGAGAGTGAACTTCAAAGGGTATGCGCTCTTTATCGCG
CTCTGGAGCATTTTTGTCTACAGCCCCATCTGTCACTGGGTATGGGCTGGCGACGGATTCCTTTTCAACCTTGGAGCAAA
GGGCGCCATCGATTTTGCCGGCGGTACCGTTGTTCATATCTCATCGGGCGTTACAGCCCTTGTTGCTGCGCTCTACCTTG
GAAAAAGACGCGGTTATCCCAAAAACGTCATGCACCCCAACAACCTCGTCATGACCCTTACCGGAGCAGGACTCTTGTGG
GTGGGCTGGTTTGGATTCAACGCCGGCAGCGCGATTGCCAGTAACCTTGACACCGCAAGAGCCTTGACCGTCACGCAGGT
TGCCGCAGCGGCAGGAGCATTCACCTGGATGATCATCGAGATGTTCCAGCATGGCAAAGCGACCAGTCTTGGCGTCGCAT
CGGGAATTCTTGCAGGACTTGTTGCCATCACGCCGGCGGCAGGCGTCGTTCAGCCGGCAGGAGCTTTTGCTCTCGGCGCG
CTTGCGGCAGTCTTCTGTTACACCGCGATCGTGCTTAAAAACAAGATCGGCTACGACGACAGTCTCGACGCATTCGGCGT
TCACGGAGTCGGCGGTATCGTTGGAGCTCTCGCCCTGACCTTTTTTATCCGTCCCGCATGGATGCTTGACGCAGCGGAAA
AAGCAGGCGGAAGCTGGACCGTATGGCAGCAGTTCGGAGTACAGGCAACGGCGGTAGGAGTTACCATTGCCTACGCGGCA
GTGATCTCTTTCATTCTTCTCTTCATCGTCGAAAAAACGGTTGGCCTGCGTATCTCCGAAGACGAAGAGATGTCGGGTCT
TGATCACAGCATGCACGGCGAGCATGGATATGGCCTTATCAACCTTAACTAA

Upstream 100 bases:

>100_bases
GTAATAAAGTATGCTTAAATGTTTGTTTTAGTTAAAATCTTAACTACATTATCGTAGTTAGCATTAATTATTAAGGCTTT
TCTAAACATAAAACAATCGC

Downstream 100 bases:

>100_bases
TTCGTTGCAATGAAACTGATTACAGCAATTATCCCGCCAGATCGGCTTGACCATGTACGGGAGGCACTGATACAGGCGGA
TATCACGAGAATTACCGTGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MMKKISGSLLFLLAVMMFSGTVHAADEVVTDTGTTSWMLTSTALVLLMVPGLAMFYGGLVRTKNVIGTMMHSFAAMVIIG
VLWPLVGYSLSFGPSILGGLAGWDNKFFMLQGIDESIMSTQIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYALFIA
LWSIFVYSPICHWVWAGDGFLFNLGAKGAIDFAGGTVVHISSGVTALVAALYLGKRRGYPKNVMHPNNLVMTLTGAGLLW
VGWFGFNAGSAIASNLDTARALTVTQVAAAAGAFTWMIIEMFQHGKATSLGVASGILAGLVAITPAAGVVQPAGAFALGA
LAAVFCYTAIVLKNKIGYDDSLDAFGVHGVGGIVGALALTFFIRPAWMLDAAEKAGGSWTVWQQFGVQATAVGVTIAYAA
VISFILLFIVEKTVGLRISEDEEMSGLDHSMHGEHGYGLINLN

Sequences:

>Translated_443_residues
MMKKISGSLLFLLAVMMFSGTVHAADEVVTDTGTTSWMLTSTALVLLMVPGLAMFYGGLVRTKNVIGTMMHSFAAMVIIG
VLWPLVGYSLSFGPSILGGLAGWDNKFFMLQGIDESIMSTQIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYALFIA
LWSIFVYSPICHWVWAGDGFLFNLGAKGAIDFAGGTVVHISSGVTALVAALYLGKRRGYPKNVMHPNNLVMTLTGAGLLW
VGWFGFNAGSAIASNLDTARALTVTQVAAAAGAFTWMIIEMFQHGKATSLGVASGILAGLVAITPAAGVVQPAGAFALGA
LAAVFCYTAIVLKNKIGYDDSLDAFGVHGVGGIVGALALTFFIRPAWMLDAAEKAGGSWTVWQQFGVQATAVGVTIAYAA
VISFILLFIVEKTVGLRISEDEEMSGLDHSMHGEHGYGLINLN
>Mature_443_residues
MMKKISGSLLFLLAVMMFSGTVHAADEVVTDTGTTSWMLTSTALVLLMVPGLAMFYGGLVRTKNVIGTMMHSFAAMVIIG
VLWPLVGYSLSFGPSILGGLAGWDNKFFMLQGIDESIMSTQIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYALFIA
LWSIFVYSPICHWVWAGDGFLFNLGAKGAIDFAGGTVVHISSGVTALVAALYLGKRRGYPKNVMHPNNLVMTLTGAGLLW
VGWFGFNAGSAIASNLDTARALTVTQVAAAAGAFTWMIIEMFQHGKATSLGVASGILAGLVAITPAAGVVQPAGAFALGA
LAAVFCYTAIVLKNKIGYDDSLDAFGVHGVGGIVGALALTFFIRPAWMLDAAEKAGGSWTVWQQFGVQATAVGVTIAYAA
VISFILLFIVEKTVGLRISEDEEMSGLDHSMHGEHGYGLINLN

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=439, Percent_Identity=40.0911161731207, Blast_Score=282, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI32564042, Length=420, Percent_Identity=28.3333333333333, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=27.7389277389277, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=28.175519630485, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=25.5707762557078, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=426, Percent_Identity=32.1596244131455, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324187, Length=428, Percent_Identity=34.8130841121495, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=426, Percent_Identity=30.9859154929577, Blast_Score=202, Evalue=7e-53,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=32.1256038647343, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46772; Mature: 46772

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKISGSLLFLLAVMMFSGTVHAADEVVTDTGTTSWMLTSTALVLLMVPGLAMFYGGLV
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
RTKNVIGTMMHSFAAMVIIGVLWPLVGYSLSFGPSILGGLAGWDNKFFMLQGIDESIMST
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHH
QIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYALFIALWSIFVYSPICHWVWAGDGF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCE
LFNLGAKGAIDFAGGTVVHISSGVTALVAALYLGKRRGYPKNVMHPNNLVMTLTGAGLLW
EEECCCCCEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
VGWFGFNAGSAIASNLDTARALTVTQVAAAAGAFTWMIIEMFQHGKATSLGVASGILAGL
EEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VAITPAAGVVQPAGAFALGALAAVFCYTAIVLKNKIGYDDSLDAFGVHGVGGIVGALALT
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
FFIRPAWMLDAAEKAGGSWTVWQQFGVQATAVGVTIAYAAVISFILLFIVEKTVGLRISE
HHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
DEEMSGLDHSMHGEHGYGLINLN
CCHHCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MMKKISGSLLFLLAVMMFSGTVHAADEVVTDTGTTSWMLTSTALVLLMVPGLAMFYGGLV
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
RTKNVIGTMMHSFAAMVIIGVLWPLVGYSLSFGPSILGGLAGWDNKFFMLQGIDESIMST
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHH
QIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYALFIALWSIFVYSPICHWVWAGDGF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCE
LFNLGAKGAIDFAGGTVVHISSGVTALVAALYLGKRRGYPKNVMHPNNLVMTLTGAGLLW
EEECCCCCEEEECCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
VGWFGFNAGSAIASNLDTARALTVTQVAAAAGAFTWMIIEMFQHGKATSLGVASGILAGL
EEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VAITPAAGVVQPAGAFALGALAAVFCYTAIVLKNKIGYDDSLDAFGVHGVGGIVGALALT
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
FFIRPAWMLDAAEKAGGSWTVWQQFGVQATAVGVTIAYAAVISFILLFIVEKTVGLRISE
HHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
DEEMSGLDHSMHGEHGYGLINLN
CCHHCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]