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The map label for this gene is lacA [C]

Identifier: 119358386

GI number: 119358386

Start: 3004133

End: 3004687

Strand: Reverse

Name: lacA [C]

Synonym: Cpha266_2619

Alternate gene names: 119358386

Gene position: 3004687-3004133 (Counterclockwise)

Preceding gene: 119358387

Following gene: 119358385

Centisome position: 95.88

GC content: 32.07

Gene sequence:

>555_bases
ATGAAAAAAGTTGTATTTGAGAGACCCTCAAAGATTAAATATACTATATATATATTAAGATCAGTTTTCTCTATTGAGTT
TATTAAACATCTTGCTTATTTGTTTGTATATTTTATAATCAACTTTACAAGAGGAAGATGTATTGCAAAAATCGGAGTTA
ATACAAAGTTGCATCCTACCGTATTAATGAGAGAGGCTGAACGTATCATAATCGGTGATAATTGCTTAATTAATCATAAT
AATGTATTACAAGCAGGGAAAAAAGATGCTAAAATTGTAATTGGAAATTATGTTCATACTGGCCCAAATGTTATGATGTT
TGCATACAATCATGTATTTGATGATCCTCATATACCTTCCAAAAATCAACAATATTATGAAGGGGATATAATTATATGTG
ATGACGTATGGATAGGTGCTGGATCTATCATTCTTGCTGGCGTGACAATAGGAAAGGGTGTTGTTGTTGCTGCGGGTACA
GTTGTGAATAAAAATATCACGCCGTTTACAGTGGTTGGTGGGGTACCTGCTAAAGTCTTAAAAATTAGAGAATAG

Upstream 100 bases:

>100_bases
TGGGTATGAAAACTTAATTGGAGGAATGGTATTGGTTGATGCATAATTGATTTTATTAAAAATGATCGTTTAATTCTGCA
ACCCTTAAGTTTCAATAAAT

Downstream 100 bases:

>100_bases
GATACTATTTTAAACGAATAATATTTATTATGGCTAATGTGTTATTGCTGGATGCTCATACAATACAAGCTCTTCCTGTT
ATGAAGGCTCTGAAAAAATC

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKKVVFERPSKIKYTIYILRSVFSIEFIKHLAYLFVYFIINFTRGRCIAKIGVNTKLHPTVLMREAERIIIGDNCLINHN
NVLQAGKKDAKIVIGNYVHTGPNVMMFAYNHVFDDPHIPSKNQQYYEGDIIICDDVWIGAGSIILAGVTIGKGVVVAAGT
VVNKNITPFTVVGGVPAKVLKIRE

Sequences:

>Translated_184_residues
MKKVVFERPSKIKYTIYILRSVFSIEFIKHLAYLFVYFIINFTRGRCIAKIGVNTKLHPTVLMREAERIIIGDNCLINHN
NVLQAGKKDAKIVIGNYVHTGPNVMMFAYNHVFDDPHIPSKNQQYYEGDIIICDDVWIGAGSIILAGVTIGKGVVVAAGT
VVNKNITPFTVVGGVPAKVLKIRE
>Mature_184_residues
MKKVVFERPSKIKYTIYILRSVFSIEFIKHLAYLFVYFIINFTRGRCIAKIGVNTKLHPTVLMREAERIIIGDNCLINHN
NVLQAGKKDAKIVIGNYVHTGPNVMMFAYNHVFDDPHIPSKNQQYYEGDIIICDDVWIGAGSIILAGVTIGKGVVVAAGT
VVNKNITPFTVVGGVPAKVLKIRE

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=133, Percent_Identity=35.3383458646617, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322243, Length=126, Percent_Identity=38.8888888888889, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20533; Mature: 20533

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVVFERPSKIKYTIYILRSVFSIEFIKHLAYLFVYFIINFTRGRCIAKIGVNTKLHPT
CCCCCCCCCCCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
VLMREAERIIIGDNCLINHNNVLQAGKKDAKIVIGNYVHTGPNVMMFAYNHVFDDPHIPS
HEEECCCEEEECCEEEEECCCHHHCCCCCCEEEEECEEECCCCEEEEEEEEECCCCCCCC
KNQQYYEGDIIICDDVWIGAGSIILAGVTIGKGVVVAAGTVVNKNITPFTVVGGVPAKVL
CCCCEEECCEEEECCCEECCCCEEEEEEEECCCEEEEECEEECCCCCCEEEECCCCEEEE
KIRE
EECC
>Mature Secondary Structure
MKKVVFERPSKIKYTIYILRSVFSIEFIKHLAYLFVYFIINFTRGRCIAKIGVNTKLHPT
CCCCCCCCCCCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
VLMREAERIIIGDNCLINHNNVLQAGKKDAKIVIGNYVHTGPNVMMFAYNHVFDDPHIPS
HEEECCCEEEECCEEEEECCCHHHCCCCCCEEEEECEEECCCCEEEEEEEEECCCCCCCC
KNQQYYEGDIIICDDVWIGAGSIILAGVTIGKGVVVAAGTVVNKNITPFTVVGGVPAKVL
CCCCEEECCEEEECCCEECCCCEEEEEEEECCCEEEEECEEECCCCCCEEEECCCCEEEE
KIRE
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]