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The map label for this gene is pfkA2 [H]

Identifier: 119358365

GI number: 119358365

Start: 2977578

End: 2978612

Strand: Reverse

Name: pfkA2 [H]

Synonym: Cpha266_2597

Alternate gene names: 119358365

Gene position: 2978612-2977578 (Counterclockwise)

Preceding gene: 119358366

Following gene: 119358364

Centisome position: 95.04

GC content: 46.86

Gene sequence:

>1035_bases
ATGATATTGTGTCACATGGTAGATAATAAAAATATGAGGGATGATTCGCACAGGAAGCCGAGGTTACAGAAAATCGGCGT
TTTTACTTCTGGCGGCGATGCTCCAGGGATGAATGCTGCTATCCGGGCTGTGACTCGGGCGGCAATTGCTAATAAATTGA
AGGTTGTGGGTATTCGTCGCGGGTATCAGGGGATGATCGAGGGAGATTTTATTCCGCTGAGGTCGGCTGATGTCAGCGGC
ATTATCCAGTTGGGGGGGACTGTTTTGAGAACGGCTCGCAGTAATGAATTCAGAACTTTCGAAGGTCGCCAGAAAGCCTG
GTTGCAGTTGCAGAAGGCTGGTATTGATGCGGTGGTGGTGATTGGGGGTGATGGTTCTTTTACTGGCGCGTTGACGATGT
CGCAGGAGTTCAATATTTCGTTTGTGGGTATTCCTGCTACTATTGATAATGATATGTATGGTACGGATTATACTATCGGT
TATGAAACGGCGTTGAATTCTGTTGTGTGTGCCGTCGATAAAATCAGGGATACGGCTGTTTCGCATGGGAGAATTTTTAT
TGTTGAGGTTATGGGTCGTGAGGCTGGTTTGCTGGCTTTGAACGGGGGTATTGCCTGCGGGGCTGAGGTTATTTTGATTC
CTGAGTCGAAGGAGCAGCATGATGAGTTGAAGCGGTTTCTTGATAAGGGTTACCGGCATAAGGAGCGGAGCGGGATTATT
ATGGTTGCTGAGGGTGATGAGCCTGGAGGTGCTTTGAAAATTGCTGAGCAGGTGCGCAGGGAGCATCCTGATCTTGATGT
GCGTGTTTCTATTTTGGGTCATATTCAGCGTGGTGGTAGTCCGACGGCTAATGACAGGGTTAATGCGACGCGGATGGGTG
TTGCTGCTGTTGATGCTTTGCTTGATGATCAGAAGAGTATTATGATCGGGATTGATGATGGTAAAATTGTGCAGGTTCCG
TTTAATAAGGCTGTGAAGTTGAATCATAGTATTGATGGTGATTTGCTGGATATTCAGAGGATGTTGAATATTTAA

Upstream 100 bases:

>100_bases
GGGAAATAATGAGGAGTGCCTGGTGCCGGATATTGGGGAGTGGTTAATATTTTCGTAAGATGGGTTTGTGGAATTAAGGG
GGCGGGTGCCTGTTTTAGTA

Downstream 100 bases:

>100_bases
GGGGATCGATTTTGCTGCGTGTTAAGTGGGACGTGGGAGTGAAGAGGGGGAGGTTGCCCGCGAATTGACGCGAATTAGCG
CGAAAAAGAAATTGGCAGTT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MILCHMVDNKNMRDDSHRKPRLQKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVVGIRRGYQGMIEGDFIPLRSADVSG
IIQLGGTVLRTARSNEFRTFEGRQKAWLQLQKAGIDAVVVIGGDGSFTGALTMSQEFNISFVGIPATIDNDMYGTDYTIG
YETALNSVVCAVDKIRDTAVSHGRIFIVEVMGREAGLLALNGGIACGAEVILIPESKEQHDELKRFLDKGYRHKERSGII
MVAEGDEPGGALKIAEQVRREHPDLDVRVSILGHIQRGGSPTANDRVNATRMGVAAVDALLDDQKSIMIGIDDGKIVQVP
FNKAVKLNHSIDGDLLDIQRMLNI

Sequences:

>Translated_344_residues
MILCHMVDNKNMRDDSHRKPRLQKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVVGIRRGYQGMIEGDFIPLRSADVSG
IIQLGGTVLRTARSNEFRTFEGRQKAWLQLQKAGIDAVVVIGGDGSFTGALTMSQEFNISFVGIPATIDNDMYGTDYTIG
YETALNSVVCAVDKIRDTAVSHGRIFIVEVMGREAGLLALNGGIACGAEVILIPESKEQHDELKRFLDKGYRHKERSGII
MVAEGDEPGGALKIAEQVRREHPDLDVRVSILGHIQRGGSPTANDRVNATRMGVAAVDALLDDQKSIMIGIDDGKIVQVP
FNKAVKLNHSIDGDLLDIQRMLNI
>Mature_344_residues
MILCHMVDNKNMRDDSHRKPRLQKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVVGIRRGYQGMIEGDFIPLRSADVSG
IIQLGGTVLRTARSNEFRTFEGRQKAWLQLQKAGIDAVVVIGGDGSFTGALTMSQEFNISFVGIPATIDNDMYGTDYTIG
YETALNSVVCAVDKIRDTAVSHGRIFIVEVMGREAGLLALNGGIACGAEVILIPESKEQHDELKRFLDKGYRHKERSGII
MVAEGDEPGGALKIAEQVRREHPDLDVRVSILGHIQRGGSPTANDRVNATRMGVAAVDALLDDQKSIMIGIDDGKIVQVP
FNKAVKLNHSIDGDLLDIQRMLNI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=344, Percent_Identity=40.9883720930233, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI266453619, Length=343, Percent_Identity=39.6501457725948, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI266453768, Length=343, Percent_Identity=39.3586005830904, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI266453748, Length=343, Percent_Identity=39.3586005830904, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI4505749, Length=343, Percent_Identity=39.3586005830904, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI48762920, Length=350, Percent_Identity=37.7142857142857, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1790350, Length=324, Percent_Identity=44.1358024691358, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25147584, Length=350, Percent_Identity=41.4285714285714, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17558788, Length=321, Percent_Identity=35.5140186915888, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI72003601, Length=305, Percent_Identity=29.5081967213115, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321679, Length=345, Percent_Identity=43.1884057971014, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323861, Length=352, Percent_Identity=38.9204545454545, Blast_Score=210, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17647809, Length=349, Percent_Identity=42.4068767908309, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24652337, Length=342, Percent_Identity=42.3976608187134, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI28573326, Length=342, Percent_Identity=41.812865497076, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37315; Mature: 37315

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILCHMVDNKNMRDDSHRKPRLQKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVVGIRR
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEC
GYQGMIEGDFIPLRSADVSGIIQLGGTVLRTARSNEFRTFEGRQKAWLQLQKAGIDAVVV
CCCCCCCCCEEEECCCCCHHHHHHCCCEEEECCCCCCEECCCCHHHHHHHHHCCCCEEEE
IGGDGSFTGALTMSQEFNISFVGIPATIDNDMYGTDYTIGYETALNSVVCAVDKIRDTAV
ECCCCCEEEEEEECCCCCEEEEEECEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHC
SHGRIFIVEVMGREAGLLALNGGIACGAEVILIPESKEQHDELKRFLDKGYRHKERSGII
CCCEEEEEEEECCCCCEEEECCCEECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEE
MVAEGDEPGGALKIAEQVRREHPDLDVRVSILGHIQRGGSPTANDRVNATRMGVAAVDAL
EEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHH
LDDQKSIMIGIDDGKIVQVPFNKAVKLNHSIDGDLLDIQRMLNI
HCCCCEEEEEECCCEEEEECCCCEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MILCHMVDNKNMRDDSHRKPRLQKIGVFTSGGDAPGMNAAIRAVTRAAIANKLKVVGIRR
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEEC
GYQGMIEGDFIPLRSADVSGIIQLGGTVLRTARSNEFRTFEGRQKAWLQLQKAGIDAVVV
CCCCCCCCCEEEECCCCCHHHHHHCCCEEEECCCCCCEECCCCHHHHHHHHHCCCCEEEE
IGGDGSFTGALTMSQEFNISFVGIPATIDNDMYGTDYTIGYETALNSVVCAVDKIRDTAV
ECCCCCEEEEEEECCCCCEEEEEECEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHC
SHGRIFIVEVMGREAGLLALNGGIACGAEVILIPESKEQHDELKRFLDKGYRHKERSGII
CCCEEEEEEEECCCCCEEEECCCEECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEE
MVAEGDEPGGALKIAEQVRREHPDLDVRVSILGHIQRGGSPTANDRVNATRMGVAAVDAL
EEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHH
LDDQKSIMIGIDDGKIVQVPFNKAVKLNHSIDGDLLDIQRMLNI
HCCCCEEEEEECCCEEEEECCCCEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]