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The map label for this gene is rpoA

Identifier: 119358164

GI number: 119358164

Start: 2783943

End: 2784926

Strand: Reverse

Name: rpoA

Synonym: Cpha266_2396

Alternate gene names: 119358164

Gene position: 2784926-2783943 (Counterclockwise)

Preceding gene: 119358165

Following gene: 119358163

Centisome position: 88.86

GC content: 42.38

Gene sequence:

>984_bases
ATGATATACCAAATGCAGATGCCTGCTAAAATAGAGGTTGACGAAGCTACGCATACGGAGATGTTTGGAAGGTTTATTGC
CCAGCCTCTTGAAAGAGGGTATGGGGTGACGATTGGTAATGTAATGCGCAGAGTATTGCTTGCCTCGCTCCCGGGAACAG
CGATAACGGGCGTGAAAATCGAGGGAGTGTATCATGAGTTTTCTGCAATTGAGGGCGTGAGAGAGGATGTTCCCGAAATT
GTTCTGAATCTCAAAAGAGTAAGGTTTAAATCAAACTGTAAAAGAAGTTGCAAGACATCGCTCTCTATTGCAGGTACGAA
AGACTTTACTGCTGGTGACATTATTGCCAAGGAAGGCGAGTTTGAGGTTTTGAATAAAGATCTCCATATCGCAACGGTCA
ATGACGGTTCTGTTCTTAATATAGAGATATATATCGGCAGAGGCAGAGGTTATCTTCCTGCTGAAGAGAATCATCCGGAA
GGAATGCCGATAGGGTTTATAGCTATCGACGCTATTTTTACTCCGATAAGAAATGTAAAGTTTACGGTTGAAAATACCCG
GGTAGGTCAAAGAACCGATTACGAGAAGATGATTCTCGATGTTGAAACTGATGGATCGATAGCCCCTGATGATTCGATCA
GTCTTGCAGGCAAGATCATCAATGATCATATCATGTATTTTGCAAACTTTTCGCCTACTGAGGAAGAGTTTACTGAGGAG
GAGTTCAAGCAGCAGGACGACGAGTTCGAGAGTATGCGCAAACTTCTTCATACGAAAATAGAGGATCTCGATCTTTCCGT
AAGATCGCATAACTGCTTGAGATTGGCGGAGATCGATACGATTGGCGATCTTGTTTCAAGAAAAGAGGATGAGCTGCTTA
ACTATAAGAATTTCGGAAAAAAGTCTTTGACAGAGCTCAAAGAGCAGCTTGAAAAGTTTGATCTGAAATTTGGGATGGAT
ATCACCAGATATCAGATGAAGTGA

Upstream 100 bases:

>100_bases
AGTGTTCCTGAAAGAGAGGAAGTGCAGGAGCCGTTTAACGAGCAGCTTGTTGTCGAGCTCTACTCGAAGTGATTTTAATG
AATACTAAGGTATAAATACT

Downstream 100 bases:

>100_bases
GAAAAGTTTAAAGGAAAATTTTTGAATATTTGAGATCAGGAATTACTCATGCGTAAAGTCAAGCCGGCAAGAAAACTCGG
GAGAACTGCTGCACATAGAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIYQMQMPAKIEVDEATHTEMFGRFIAQPLERGYGVTIGNVMRRVLLASLPGTAITGVKIEGVYHEFSAIEGVREDVPEI
VLNLKRVRFKSNCKRSCKTSLSIAGTKDFTAGDIIAKEGEFEVLNKDLHIATVNDGSVLNIEIYIGRGRGYLPAEENHPE
GMPIGFIAIDAIFTPIRNVKFTVENTRVGQRTDYEKMILDVETDGSIAPDDSISLAGKIINDHIMYFANFSPTEEEFTEE
EFKQQDDEFESMRKLLHTKIEDLDLSVRSHNCLRLAEIDTIGDLVSRKEDELLNYKNFGKKSLTELKEQLEKFDLKFGMD
ITRYQMK

Sequences:

>Translated_327_residues
MIYQMQMPAKIEVDEATHTEMFGRFIAQPLERGYGVTIGNVMRRVLLASLPGTAITGVKIEGVYHEFSAIEGVREDVPEI
VLNLKRVRFKSNCKRSCKTSLSIAGTKDFTAGDIIAKEGEFEVLNKDLHIATVNDGSVLNIEIYIGRGRGYLPAEENHPE
GMPIGFIAIDAIFTPIRNVKFTVENTRVGQRTDYEKMILDVETDGSIAPDDSISLAGKIINDHIMYFANFSPTEEEFTEE
EFKQQDDEFESMRKLLHTKIEDLDLSVRSHNCLRLAEIDTIGDLVSRKEDELLNYKNFGKKSLTELKEQLEKFDLKFGMD
ITRYQMK
>Mature_327_residues
MIYQMQMPAKIEVDEATHTEMFGRFIAQPLERGYGVTIGNVMRRVLLASLPGTAITGVKIEGVYHEFSAIEGVREDVPEI
VLNLKRVRFKSNCKRSCKTSLSIAGTKDFTAGDIIAKEGEFEVLNKDLHIATVNDGSVLNIEIYIGRGRGYLPAEENHPE
GMPIGFIAIDAIFTPIRNVKFTVENTRVGQRTDYEKMILDVETDGSIAPDDSISLAGKIINDHIMYFANFSPTEEEFTEE
EFKQQDDEFESMRKLLHTKIEDLDLSVRSHNCLRLAEIDTIGDLVSRKEDELLNYKNFGKKSLTELKEQLEKFDLKFGMD
ITRYQMK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=321, Percent_Identity=37.6947040498442, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CHLPD (A1BJ07)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912808.1
- ProteinModelPortal:   A1BJ07
- STRING:   A1BJ07
- GeneID:   4568618
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2396
- NMPDR:   fig|290317.7.peg.2512
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37133; Mature: 37133

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYQMQMPAKIEVDEATHTEMFGRFIAQPLERGYGVTIGNVMRRVLLASLPGTAITGVKI
CEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEECEEE
EGVYHEFSAIEGVREDVPEIVLNLKRVRFKSNCKRSCKTSLSIAGTKDFTAGDIIAKEGE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEECCCC
FEVLNKDLHIATVNDGSVLNIEIYIGRGRGYLPAEENHPEGMPIGFIAIDAIFTPIRNVK
CEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEE
FTVENTRVGQRTDYEKMILDVETDGSIAPDDSISLAGKIINDHIMYFANFSPTEEEFTEE
EEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHH
EFKQQDDEFESMRKLLHTKIEDLDLSVRSHNCLRLAEIDTIGDLVSRKEDELLNYKNFGK
HHHHCCHHHHHHHHHHHHHHHHCEEEECCCCEEEEEHHHHHHHHHHCCHHHHHCHHHCCH
KSLTELKEQLEKFDLKFGMDITRYQMK
HHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MIYQMQMPAKIEVDEATHTEMFGRFIAQPLERGYGVTIGNVMRRVLLASLPGTAITGVKI
CEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEECEEE
EGVYHEFSAIEGVREDVPEIVLNLKRVRFKSNCKRSCKTSLSIAGTKDFTAGDIIAKEGE
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCEEECCCC
FEVLNKDLHIATVNDGSVLNIEIYIGRGRGYLPAEENHPEGMPIGFIAIDAIFTPIRNVK
CEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEE
FTVENTRVGQRTDYEKMILDVETDGSIAPDDSISLAGKIINDHIMYFANFSPTEEEFTEE
EEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHH
EFKQQDDEFESMRKLLHTKIEDLDLSVRSHNCLRLAEIDTIGDLVSRKEDELLNYKNFGK
HHHHCCHHHHHHHHHHHHHHHHCEEEECCCCEEEEEHHHHHHHHHHCCHHHHHCHHHCCH
KSLTELKEQLEKFDLKFGMDITRYQMK
HHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA