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The map label for this gene is dfrA [H]

Identifier: 119358062

GI number: 119358062

Start: 2621313

End: 2622302

Strand: Reverse

Name: dfrA [H]

Synonym: Cpha266_2290

Alternate gene names: 119358062

Gene position: 2622302-2621313 (Counterclockwise)

Preceding gene: 119358065

Following gene: 119358061

Centisome position: 83.68

GC content: 52.42

Gene sequence:

>990_bases
ATGCAGGAGAGATTACTACTTATAACTGGCGCTACAGGCTATATTGGTTCTCAGGTTGTTGCGGCACTGCTTGAAAGATT
TTCTGATGAGTTTCGTTGCAGGGTTGTTGCCAGAAAAACTTCGGATTGCAGCTTTCTTGACGACTTGCCGGTAGAGATTG
TCAGGGCCGACATAGAAGAGCCGGTGGCGCTTTTTGATGCGTTCAGCGGGGTTGATACGGTTTTTCATTGTGCAGGTCTG
ATTTCCTACTCTCGCCATTTTCGGAACCCGCTTTATGACGTCAACGTTATCGGGACACGAAATGTTGTTAACGCCTGTAT
TGCCAACAGGGTTCGCCGCCTCGTCATGACCAGTTCCATTGCTGCTCTTGGAGTCGCCGAGGACGGTTCCAGGGTAGTTG
AGTCAACGTCTTTCAAAGAGATGCCCCACCGTAATGGCTACATGGAGGCAAAGCACCTTGCGGAGCTTGAAGGGTTACGG
GGGATTGCCGAAGGTCTTGATGTCGTTATGGTGAATCCCGGTGTGGTGATTGGCGTCGACTCCGCCAACAGTGCTTCATC
GAGTTCCTCAAATGATGTGTTGCGGATGATCTATCGCGGACAACTGCCGTTCTGCCCTTCAGGCGCGACAGGCTTTGTCG
ATGTTCGTGATACGGCGGATGCGCTTCTTCTCGCCTGGCAAAAGGGAGAGCGCGGAGCGCGTTATATTGCAGTGGGCCAT
AACCTTTTGTTCAGGGAGCTGTTTGATGCGCTTGGCCGGATCCCTGGAAACAGCATGAAACGGGTGTACGCTCTCCCTGG
AGTTCTCGGGTATGCGGCAGGGCTTGCCGGCGAGGCATTTTCGTTTGTTCTCAACAGGCCTTCGGTTATCAGTATCGACA
GCATTCGCCTGTCGGCCATGCAACTTTCTTTTAATAACAGCCGTTCAGTTCAGGAGCTTGGTATGCGTTACCGGTCTCTT
GATGAAACCCTGCGGAGTGCGGTACGCTGA

Upstream 100 bases:

>100_bases
TGCTGGAACTTGGCATATATCGTGAGATATCCTGTTTTCTGCGGCTGGCCAAAAAAGGCTTGTTCGTAAAAGAGCGAAGA
GGGCTAATGTCTGTTTCCTT

Downstream 100 bases:

>100_bases
AAGATTTTAATTTCACTCAATTTACAGTTACGATGATATGGACAATATAAAGACAGAGCAGAAATCCGATCTGGTCGATC
CGGCGAAACTCAAACAGTTG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MQERLLLITGATGYIGSQVVAALLERFSDEFRCRVVARKTSDCSFLDDLPVEIVRADIEEPVALFDAFSGVDTVFHCAGL
ISYSRHFRNPLYDVNVIGTRNVVNACIANRVRRLVMTSSIAALGVAEDGSRVVESTSFKEMPHRNGYMEAKHLAELEGLR
GIAEGLDVVMVNPGVVIGVDSANSASSSSSNDVLRMIYRGQLPFCPSGATGFVDVRDTADALLLAWQKGERGARYIAVGH
NLLFRELFDALGRIPGNSMKRVYALPGVLGYAAGLAGEAFSFVLNRPSVISIDSIRLSAMQLSFNNSRSVQELGMRYRSL
DETLRSAVR

Sequences:

>Translated_329_residues
MQERLLLITGATGYIGSQVVAALLERFSDEFRCRVVARKTSDCSFLDDLPVEIVRADIEEPVALFDAFSGVDTVFHCAGL
ISYSRHFRNPLYDVNVIGTRNVVNACIANRVRRLVMTSSIAALGVAEDGSRVVESTSFKEMPHRNGYMEAKHLAELEGLR
GIAEGLDVVMVNPGVVIGVDSANSASSSSSNDVLRMIYRGQLPFCPSGATGFVDVRDTADALLLAWQKGERGARYIAVGH
NLLFRELFDALGRIPGNSMKRVYALPGVLGYAAGLAGEAFSFVLNRPSVISIDSIRLSAMQLSFNNSRSVQELGMRYRSL
DETLRSAVR
>Mature_329_residues
MQERLLLITGATGYIGSQVVAALLERFSDEFRCRVVARKTSDCSFLDDLPVEIVRADIEEPVALFDAFSGVDTVFHCAGL
ISYSRHFRNPLYDVNVIGTRNVVNACIANRVRRLVMTSSIAALGVAEDGSRVVESTSFKEMPHRNGYMEAKHLAELEGLR
GIAEGLDVVMVNPGVVIGVDSANSASSSSSNDVLRMIYRGQLPFCPSGATGFVDVRDTADALLLAWQKGERGARYIAVGH
NLLFRELFDALGRIPGNSMKRVYALPGVLGYAAGLAGEAFSFVLNRPSVISIDSIRLSAMQLSFNNSRSVQELGMRYRSL
DETLRSAVR

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI218563686, Length=164, Percent_Identity=32.9268292682927, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI218563684, Length=164, Percent_Identity=32.9268292682927, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI19923621, Length=163, Percent_Identity=33.1288343558282, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=343, Percent_Identity=26.2390670553936, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 35877; Mature: 35877

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERLLLITGATGYIGSQVVAALLERFSDEFRCRVVARKTSDCSFLDDLPVEIVRADIEE
CCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCHHCCCHHHHHHHHHH
PVALFDAFSGVDTVFHCAGLISYSRHFRNPLYDVNVIGTRNVVNACIANRVRRLVMTSSI
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
AALGVAEDGSRVVESTSFKEMPHRNGYMEAKHLAELEGLRGIAEGLDVVMVNPGVVIGVD
HHEEECCCCCHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
SANSASSSSSNDVLRMIYRGQLPFCPSGATGFVDVRDTADALLLAWQKGERGARYIAVGH
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEECH
NLLFRELFDALGRIPGNSMKRVYALPGVLGYAAGLAGEAFSFVLNRPSVISIDSIRLSAM
HHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEE
QLSFNNSRSVQELGMRYRSLDETLRSAVR
EEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQERLLLITGATGYIGSQVVAALLERFSDEFRCRVVARKTSDCSFLDDLPVEIVRADIEE
CCCCEEEEECCCCHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCHHCCCHHHHHHHHHH
PVALFDAFSGVDTVFHCAGLISYSRHFRNPLYDVNVIGTRNVVNACIANRVRRLVMTSSI
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
AALGVAEDGSRVVESTSFKEMPHRNGYMEAKHLAELEGLRGIAEGLDVVMVNPGVVIGVD
HHEEECCCCCHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
SANSASSSSSNDVLRMIYRGQLPFCPSGATGFVDVRDTADALLLAWQKGERGARYIAVGH
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEECH
NLLFRELFDALGRIPGNSMKRVYALPGVLGYAAGLAGEAFSFVLNRPSVISIDSIRLSAM
HHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEE
QLSFNNSRSVQELGMRYRSLDETLRSAVR
EEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]