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The map label for this gene is mcl2 [H]

Identifier: 119357979

GI number: 119357979

Start: 2529421

End: 2530368

Strand: Reverse

Name: mcl2 [H]

Synonym: Cpha266_2198

Alternate gene names: 119357979

Gene position: 2530368-2529421 (Counterclockwise)

Preceding gene: 119357980

Following gene: 119357975

Centisome position: 80.74

GC content: 54.54

Gene sequence:

>948_bases
ATGATTTTCAAAGACATTACCTGGCTGGACAGTGCCAGTATTGAAGCGATCGAACAGCAGTTGCCTGAAACTGTTTCCCG
GCGCCCCCTGACTCGCGGTCTGCGCCGAAGCGCCCTTATGGTTTCGGCCCATCGGGTCAACCATCTGAACAAACTGGATC
TACTGGCAGCAGATATGGCTGTCATCAATCTGGAAGATGGCGTTGCACCGCAAGAGAAGCCCCGCGCTCGTGCTCTGGCT
GCGCTGTTTACCGCCCATGCCCGAGAGGCGAAAACGGAACTGATTGTACGGGTCAATCCCTTGGGTGAAGGGGGTGAAGA
AGATATCCGGCTGATCAATCGGGCAGTGCCGGACGCAATCCGTATCCCCAAGGTCAGAACCGCTCAGGAAGTCGAACAGG
CTTGTCGCCTGGTAGATCCTGCGATTCGAATCCATCTCTCTGTCGAGACCGGTGAAGCACTGAAGAAACTTGCCGAGTTG
AGGGTTGAGGAACGGGTGGATACGGTCTATCTGGGTATTCTGGATTTATGTGCCGATCTCGGATTGCCCCAGTCGGTGAT
CACTCCTGGGAACCCAATGGCCAGCTATCTGCTTTCACGCTTTCTGGTGGATGCCCTCACCGCCGGCTTTCATCCGGTAT
CGTTTGTCTATCAGGAGTACAAGGATCTGGAGACCTTCGAAAAATGGTGCCTTCAGGAACGGAGCATGGGCTTTTCATCC
AAAGGCTGTATCTCTCCGGGGCAGGTGGAGCTGGCCAACCGGGTTTTCGCCCCCAAGCCAGAGGAGCGCGCGCGAGCAAT
AACCATTGTGCGCTGTTTTGAAGAGGAGGCAAAAAAGGGCAACACCGGCTTTGTGCATGAGCAGTACGGGTTTATCGACG
AGCCTGTTTACAGAGGAGCATTGGCTGTTTTGGCTGCAACCGATCATGAGAAAGTCTCAGGAATGTAA

Upstream 100 bases:

>100_bases
TATATTTCGTCAACACACCCGCACATCTTACAGGGAGAGGGGGTGACGTTCCCGACCTCTGTTTCGATGCAGGCATTTCA
TTGCAAAATCCCTTGTCCTG

Downstream 100 bases:

>100_bases
TGCGATGCGATGCTGAATATATGCCGAGGGGATGCTCACGACTACGTAAACTAAATGCAACTTCAGGGCGTTCCTCCGTA
TATCGAAATAATTCTATAAA

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MIFKDITWLDSASIEAIEQQLPETVSRRPLTRGLRRSALMVSAHRVNHLNKLDLLAADMAVINLEDGVAPQEKPRARALA
ALFTAHAREAKTELIVRVNPLGEGGEEDIRLINRAVPDAIRIPKVRTAQEVEQACRLVDPAIRIHLSVETGEALKKLAEL
RVEERVDTVYLGILDLCADLGLPQSVITPGNPMASYLLSRFLVDALTAGFHPVSFVYQEYKDLETFEKWCLQERSMGFSS
KGCISPGQVELANRVFAPKPEERARAITIVRCFEEEAKKGNTGFVHEQYGFIDEPVYRGALAVLAATDHEKVSGM

Sequences:

>Translated_315_residues
MIFKDITWLDSASIEAIEQQLPETVSRRPLTRGLRRSALMVSAHRVNHLNKLDLLAADMAVINLEDGVAPQEKPRARALA
ALFTAHAREAKTELIVRVNPLGEGGEEDIRLINRAVPDAIRIPKVRTAQEVEQACRLVDPAIRIHLSVETGEALKKLAEL
RVEERVDTVYLGILDLCADLGLPQSVITPGNPMASYLLSRFLVDALTAGFHPVSFVYQEYKDLETFEKWCLQERSMGFSS
KGCISPGQVELANRVFAPKPEERARAITIVRCFEEEAKKGNTGFVHEQYGFIDEPVYRGALAVLAATDHEKVSGM
>Mature_315_residues
MIFKDITWLDSASIEAIEQQLPETVSRRPLTRGLRRSALMVSAHRVNHLNKLDLLAADMAVINLEDGVAPQEKPRARALA
ALFTAHAREAKTELIVRVNPLGEGGEEDIRLINRAVPDAIRIPKVRTAQEVEQACRLVDPAIRIHLSVETGEALKKLAEL
RVEERVDTVYLGILDLCADLGLPQSVITPGNPMASYLLSRFLVDALTAGFHPVSFVYQEYKDLETFEKWCLQERSMGFSS
KGCISPGQVELANRVFAPKPEERARAITIVRCFEEEAKKGNTGFVHEQYGFIDEPVYRGALAVLAATDHEKVSGM

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=300, Percent_Identity=28, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87081764, Length=240, Percent_Identity=27.5, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17557458, Length=296, Percent_Identity=26.6891891891892, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 34997; Mature: 34997

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKDITWLDSASIEAIEQQLPETVSRRPLTRGLRRSALMVSAHRVNHLNKLDLLAADMA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VINLEDGVAPQEKPRARALAALFTAHAREAKTELIVRVNPLGEGGEEDIRLINRAVPDAI
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCHHH
RIPKVRTAQEVEQACRLVDPAIRIHLSVETGEALKKLAELRVEERVDTVYLGILDLCADL
CCCCCCCHHHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLPQSVITPGNPMASYLLSRFLVDALTAGFHPVSFVYQEYKDLETFEKWCLQERSMGFSS
CCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KGCISPGQVELANRVFAPKPEERARAITIVRCFEEEAKKGNTGFVHEQYGFIDEPVYRGA
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHH
LAVLAATDHEKVSGM
HHHEEECCHHHHCCC
>Mature Secondary Structure
MIFKDITWLDSASIEAIEQQLPETVSRRPLTRGLRRSALMVSAHRVNHLNKLDLLAADMA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VINLEDGVAPQEKPRARALAALFTAHAREAKTELIVRVNPLGEGGEEDIRLINRAVPDAI
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCHHH
RIPKVRTAQEVEQACRLVDPAIRIHLSVETGEALKKLAELRVEERVDTVYLGILDLCADL
CCCCCCCHHHHHHHHHHHCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLPQSVITPGNPMASYLLSRFLVDALTAGFHPVSFVYQEYKDLETFEKWCLQERSMGFSS
CCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KGCISPGQVELANRVFAPKPEERARAITIVRCFEEEAKKGNTGFVHEQYGFIDEPVYRGA
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHH
LAVLAATDHEKVSGM
HHHEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA