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The map label for this gene is fadH [C]

Identifier: 119357770

GI number: 119357770

Start: 2290110

End: 2291345

Strand: Reverse

Name: fadH [C]

Synonym: Cpha266_1977

Alternate gene names: 119357770

Gene position: 2291345-2290110 (Counterclockwise)

Preceding gene: 119357773

Following gene: 119357767

Centisome position: 73.11

GC content: 54.29

Gene sequence:

>1236_bases
ATGCGCCAGGATATTTTCGGTTCTGCTGTCATTTCAGGAATTCGACTCCAAAACCGCCTGATACGATCTGCAACGCATGA
AAGCATGGCGGAGAGCAGTGGTGCGCCTTCCGCTGCTCATGAAAAGCTTTATACGTCTCTTGCCAGGAGCGGGGTTGGCG
CAGTCATTACCGGTTATGCCGGGGTTCAGCAGGACGGACGAACCAATTATCCGGGTATGCTGATGATGCATCATGACAGT
CTCATTCCGGCATACAGGAAACTTGCCGATGCGGTTCATCGACACGGTACTCCAATCATTCTGCAGCTTGCTCACTGCGG
TCGTCAGACCCGCTCGGCGGTAACCGGCATGAAGACGGTTGCCCCTTCTGCGCTGAAGGATCGTTTTTTCAATGAAGAGC
GTCCCCAGGCCCTTGGTGAACAGGAAATAGAGGCGATCATCAGCAACTTTGTTTCAGCAGCCGGTCGGGCGGTAAGCGCC
GGTTTTGACGGCGTACAGCTCCATATGGCTCATGGCTATCTGCTTGCGGAGTTTCTTTCCGGTTATACCAATCGACGAAA
GGATCGCTGGGGTGGTTCGACAGAGAACAAGTTCCGTATTGTGGCTGAAATATTGCGTCGCATCCACAATGAACAAGGCG
ATTTTCCCGTGCTGGCCAAAATCAACGGTTTCGATCAGAGGCCCGGCGGGATGAGGGGCGAAGAAGCTGTCGATATTGCC
CGGTTGCTCGAAGCATCAGGTTGCTCCGCTATCGAGGTTTCGTCTGGCACGATCGAGGAGGGGTTGTCGATCATGAGAGG
GCCGAAGCTGCCGGTTGAACCTATACTTGCCTGTAATTTCAGGTTCAAATCGATGCCGGTGCCGCTGAAAAAAGTTCTCG
CACCGCTTCTGCCTCTGGTAATGCCCTTTCCCGACCTCATACGGAACTACAATCTGGAAGTGGCACGAGCCATCAAAAAA
GCAGTCTCGATTCCGGTGATTGCCGTCGGGGGCATTCACACCATGGAAGATATTTCGGCAGTGCTCGTCAGTGGAGCTGC
TGACTTCGTTGCCATGTCGAGACCGTTCATCATCGAGCCTGACATCGTGCGCAAACTGCGGGAGGGAAGTCAAACGGAAT
CACGCTGTATCGCTTGCAATTACTGCGCGATCATGATTGAAGAGGGGCCGCTCAGATGCTGGTACGGAAAGATGCCCGGG
ATGCAGGCCGAACGGCGTGGCTGTGTCGGCGGGTAA

Upstream 100 bases:

>100_bases
TGTGACGCTCTCTGGCGCGGTGATTTGTTCCGGAACCGTTATTTTGAATACAGGGTTTCAGAAAAATTTTTGTCACGAGG
GAAAGAGGGGATAAACTATA

Downstream 100 bases:

>100_bases
CTTCTTATGCTCATCGAGCCTCACTCATTTGCTGGAAGATGTTGTTTGACTGATGCGATGAGCGCATCAAGGTCAGCAAG
GTCGTCAATCTCCTTTTTCG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MRQDIFGSAVISGIRLQNRLIRSATHESMAESSGAPSAAHEKLYTSLARSGVGAVITGYAGVQQDGRTNYPGMLMMHHDS
LIPAYRKLADAVHRHGTPIILQLAHCGRQTRSAVTGMKTVAPSALKDRFFNEERPQALGEQEIEAIISNFVSAAGRAVSA
GFDGVQLHMAHGYLLAEFLSGYTNRRKDRWGGSTENKFRIVAEILRRIHNEQGDFPVLAKINGFDQRPGGMRGEEAVDIA
RLLEASGCSAIEVSSGTIEEGLSIMRGPKLPVEPILACNFRFKSMPVPLKKVLAPLLPLVMPFPDLIRNYNLEVARAIKK
AVSIPVIAVGGIHTMEDISAVLVSGAADFVAMSRPFIIEPDIVRKLREGSQTESRCIACNYCAIMIEEGPLRCWYGKMPG
MQAERRGCVGG

Sequences:

>Translated_411_residues
MRQDIFGSAVISGIRLQNRLIRSATHESMAESSGAPSAAHEKLYTSLARSGVGAVITGYAGVQQDGRTNYPGMLMMHHDS
LIPAYRKLADAVHRHGTPIILQLAHCGRQTRSAVTGMKTVAPSALKDRFFNEERPQALGEQEIEAIISNFVSAAGRAVSA
GFDGVQLHMAHGYLLAEFLSGYTNRRKDRWGGSTENKFRIVAEILRRIHNEQGDFPVLAKINGFDQRPGGMRGEEAVDIA
RLLEASGCSAIEVSSGTIEEGLSIMRGPKLPVEPILACNFRFKSMPVPLKKVLAPLLPLVMPFPDLIRNYNLEVARAIKK
AVSIPVIAVGGIHTMEDISAVLVSGAADFVAMSRPFIIEPDIVRKLREGSQTESRCIACNYCAIMIEEGPLRCWYGKMPG
MQAERRGCVGG
>Mature_411_residues
MRQDIFGSAVISGIRLQNRLIRSATHESMAESSGAPSAAHEKLYTSLARSGVGAVITGYAGVQQDGRTNYPGMLMMHHDS
LIPAYRKLADAVHRHGTPIILQLAHCGRQTRSAVTGMKTVAPSALKDRFFNEERPQALGEQEIEAIISNFVSAAGRAVSA
GFDGVQLHMAHGYLLAEFLSGYTNRRKDRWGGSTENKFRIVAEILRRIHNEQGDFPVLAKINGFDQRPGGMRGEEAVDIA
RLLEASGCSAIEVSSGTIEEGLSIMRGPKLPVEPILACNFRFKSMPVPLKKVLAPLLPLVMPFPDLIRNYNLEVARAIKK
AVSIPVIAVGGIHTMEDISAVLVSGAADFVAMSRPFIIEPDIVRKLREGSQTESRCIACNYCAIMIEEGPLRCWYGKMPG
MQAERRGCVGG

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=406, Percent_Identity=30.0492610837438, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787939, Length=338, Percent_Identity=27.2189349112426, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=374, Percent_Identity=29.4117647058824, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=386, Percent_Identity=27.979274611399, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=297, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=279, Percent_Identity=30.1075268817204, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=386, Percent_Identity=25.6476683937824, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=384, Percent_Identity=24.21875, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=266, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=156, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=154, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 44738; Mature: 44738

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQDIFGSAVISGIRLQNRLIRSATHESMAESSGAPSAAHEKLYTSLARSGVGAVITGYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCC
GVQQDGRTNYPGMLMMHHDSLIPAYRKLADAVHRHGTPIILQLAHCGRQTRSAVTGMKTV
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHH
APSALKDRFFNEERPQALGEQEIEAIISNFVSAAGRAVSAGFDGVQLHMAHGYLLAEFLS
CHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHCCCCCEEEEHHHHHHHHHHHH
GYTNRRKDRWGGSTENKFRIVAEILRRIHNEQGDFPVLAKINGFDQRPGGMRGEEAVDIA
HHHHCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHH
RLLEASGCSAIEVSSGTIEEGLSIMRGPKLPVEPILACNFRFKSMPVPLKKVLAPLLPLV
HHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
MPFPDLIRNYNLEVARAIKKAVSIPVIAVGGIHTMEDISAVLVSGAADFVAMSRPFIIEP
CCCHHHHHCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCEECH
DIVRKLREGSQTESRCIACNYCAIMIEEGPLRCWYGKMPGMQAERRGCVGG
HHHHHHHCCCCCHHHEEEEEEEEEEEECCCCEEEECCCCCCCHHCCCCCCC
>Mature Secondary Structure
MRQDIFGSAVISGIRLQNRLIRSATHESMAESSGAPSAAHEKLYTSLARSGVGAVITGYA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCC
GVQQDGRTNYPGMLMMHHDSLIPAYRKLADAVHRHGTPIILQLAHCGRQTRSAVTGMKTV
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHH
APSALKDRFFNEERPQALGEQEIEAIISNFVSAAGRAVSAGFDGVQLHMAHGYLLAEFLS
CHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHCCCCCEEEEHHHHHHHHHHHH
GYTNRRKDRWGGSTENKFRIVAEILRRIHNEQGDFPVLAKINGFDQRPGGMRGEEAVDIA
HHHHCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHH
RLLEASGCSAIEVSSGTIEEGLSIMRGPKLPVEPILACNFRFKSMPVPLKKVLAPLLPLV
HHHHHCCCCEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
MPFPDLIRNYNLEVARAIKKAVSIPVIAVGGIHTMEDISAVLVSGAADFVAMSRPFIIEP
CCCHHHHHCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCEECH
DIVRKLREGSQTESRCIACNYCAIMIEEGPLRCWYGKMPGMQAERRGCVGG
HHHHHHHCCCCCHHHEEEEEEEEEEEECCCCEEEECCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]