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The map label for this gene is paeR7IM [H]

Identifier: 119357705

GI number: 119357705

Start: 2216323

End: 2217996

Strand: Reverse

Name: paeR7IM [H]

Synonym: Cpha266_1909

Alternate gene names: 119357705

Gene position: 2217996-2216323 (Counterclockwise)

Preceding gene: 119357706

Following gene: 119357704

Centisome position: 70.77

GC content: 50.06

Gene sequence:

>1674_bases
ATGGTCGTTGCTGCGATTGAAAAATTGTCTTCAGCAGCAGGAACCGATTCTCGCGGCGCTATATTTACACGCGTTGAAGT
AGTTGATTTTATTCTCGATCTGGCCGGCTATACGGAGGATCAATTGCTCCATGAAAAACGGCTTTTAGAGCCATCATTCG
GTGGCGGTGACTTTCTTTTGCCGGCAATTGATCGATTACTGGCAGCGTGGAAATCATCGCAACATGTAACATCTGTCGTC
GAAGAGTTAGGTGATGCAATTCGCGCTGTTGAGCTTCATCACAAGACCTTTTCTTCCACCCGTGCAGCCGTGATCGAGAA
GTTACGACTGGAATCGATAGATGTTCAATGCGCAGCAACACTTGCTGATCGTTGGCTTGTTCATGGGGATTTTCTGCTTT
CTCCACTTGACGGGCATTTTGACTTTATTGTCGGGAACCCGCCCTATGTGCGCCAGGAGCTTATTCCGGCAGCGTTACTG
GCTGAATATCGCAATCGATATCAGACGATGTATGACCGGGCCGATATTTATATTCCGTTCATCGAGCGTTCCCTTTCAGT
ATTGGGCAAGGGTGGGAGCCTGGGTTTTATCTGTGCGGATCGCTGGATGAAAAACCGCTATGGCGGTCCGCTTCGCAGCC
TTGTGGCCAATCAGTTTCACCTGAAAATTTATGTTGATATGGTAGATACGCCAGCGTTTCTCTCTGATGTGATAGCCTAT
CCAGCCATCACTATTATCAGCAGGGAAACTCCCGGTGCGACACGCATTGCTCATCGTCCGGCCATCGACAGAGATACGTT
GGCAAACCTGGCTGATGCGCTGTGTGCTTCGAACCAGCTGAATGAGGTCGATTCGATAAGCGAACTGGCACAAGTTACTA
ATGGTGCAGAACCCTGGTTACTCGAATCTTCGGATCAGATGGCCCTGATCCGTCGTCTGGAAAAACAATTTCCCAGCCTG
GAAGAGGTTGGATGCAAGGTGGGTATAGGAGTGGCAACTGGCGCGGACAAAGCATATATCGGAGACTTTGATACACTCGA
CGTGGAGCCTGATCGCAAACTGCCGCTGGTTACAACCAAAGATATTCTGTCAGGAGAAGTGCAATGGCGCGGTCAAGGTG
TCATCAATCCTTTTGTCGAGGGTGGTGGTCTTGTTGAGTTGAGGAGTTATCCCCGCTTGCGTCGCTACCTGGAGGCTCGA
CGAGACGTTATTATGGACCGACATTGTGCACGAAAGTCTCCGGATAACTGGTATCGCACAATCGACAGGATCACCCCTGC
ACTGGCATCAAGGCCGAAACTCCTGATTCCCGATATCAAGGGAGAGGCTCATGTCGTTTATGAAGGAGGTGCACTTTATC
CTCACCATAATCTTTACTATGTTACGTCCGACGAGTGGGAGTTGAGGGCATTGCAAGCTGTGTTGCTTTCAGACGTCACC
CGTCTGTTCATGTCAACCTACTCCACCAGAATGCGTGGAGGCTACCTCCGGTTTCAGGCGCAGTACCTGCGTCGCATCCG
CATTCCTCAATGGGCGAATGTTTCGATAATGTTGCGGAAGGCGCTTGCGGAAGCTGCAATCATGAGGGATTTTCCTGCTT
GCAATCGCGCAGTATTCAAGCTATTTGAACTTGGCAATAACGAACGATCCGCCCTTGGAGGCAATGGAGAATAA

Upstream 100 bases:

>100_bases
GATTGAACCAATGCGCAAAGGTTAAATAGTATGTATGCACAAAGTTTCGAGACAACTTGATTTTGACTTTTTTGGCGAGT
CAGATTGTACTCATTCGCCG

Downstream 100 bases:

>100_bases
ATGACTCTTGATCTTGTCAATTACGAACAAAAGGCACATGAAGCCGTAAAATCCTTCTGGAGTAATCGTGAGGTGGCGAA
GCAAAAGCAGCTCCTGACAG

Product: hypothetical protein

Products: NA

Alternate protein names: M.PaeR7I; Adenine-specific methyltransferase PaeR7I [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MVVAAIEKLSSAAGTDSRGAIFTRVEVVDFILDLAGYTEDQLLHEKRLLEPSFGGGDFLLPAIDRLLAAWKSSQHVTSVV
EELGDAIRAVELHHKTFSSTRAAVIEKLRLESIDVQCAATLADRWLVHGDFLLSPLDGHFDFIVGNPPYVRQELIPAALL
AEYRNRYQTMYDRADIYIPFIERSLSVLGKGGSLGFICADRWMKNRYGGPLRSLVANQFHLKIYVDMVDTPAFLSDVIAY
PAITIISRETPGATRIAHRPAIDRDTLANLADALCASNQLNEVDSISELAQVTNGAEPWLLESSDQMALIRRLEKQFPSL
EEVGCKVGIGVATGADKAYIGDFDTLDVEPDRKLPLVTTKDILSGEVQWRGQGVINPFVEGGGLVELRSYPRLRRYLEAR
RDVIMDRHCARKSPDNWYRTIDRITPALASRPKLLIPDIKGEAHVVYEGGALYPHHNLYYVTSDEWELRALQAVLLSDVT
RLFMSTYSTRMRGGYLRFQAQYLRRIRIPQWANVSIMLRKALAEAAIMRDFPACNRAVFKLFELGNNERSALGGNGE

Sequences:

>Translated_557_residues
MVVAAIEKLSSAAGTDSRGAIFTRVEVVDFILDLAGYTEDQLLHEKRLLEPSFGGGDFLLPAIDRLLAAWKSSQHVTSVV
EELGDAIRAVELHHKTFSSTRAAVIEKLRLESIDVQCAATLADRWLVHGDFLLSPLDGHFDFIVGNPPYVRQELIPAALL
AEYRNRYQTMYDRADIYIPFIERSLSVLGKGGSLGFICADRWMKNRYGGPLRSLVANQFHLKIYVDMVDTPAFLSDVIAY
PAITIISRETPGATRIAHRPAIDRDTLANLADALCASNQLNEVDSISELAQVTNGAEPWLLESSDQMALIRRLEKQFPSL
EEVGCKVGIGVATGADKAYIGDFDTLDVEPDRKLPLVTTKDILSGEVQWRGQGVINPFVEGGGLVELRSYPRLRRYLEAR
RDVIMDRHCARKSPDNWYRTIDRITPALASRPKLLIPDIKGEAHVVYEGGALYPHHNLYYVTSDEWELRALQAVLLSDVT
RLFMSTYSTRMRGGYLRFQAQYLRRIRIPQWANVSIMLRKALAEAAIMRDFPACNRAVFKLFELGNNERSALGGNGE
>Mature_557_residues
MVVAAIEKLSSAAGTDSRGAIFTRVEVVDFILDLAGYTEDQLLHEKRLLEPSFGGGDFLLPAIDRLLAAWKSSQHVTSVV
EELGDAIRAVELHHKTFSSTRAAVIEKLRLESIDVQCAATLADRWLVHGDFLLSPLDGHFDFIVGNPPYVRQELIPAALL
AEYRNRYQTMYDRADIYIPFIERSLSVLGKGGSLGFICADRWMKNRYGGPLRSLVANQFHLKIYVDMVDTPAFLSDVIAY
PAITIISRETPGATRIAHRPAIDRDTLANLADALCASNQLNEVDSISELAQVTNGAEPWLLESSDQMALIRRLEKQFPSL
EEVGCKVGIGVATGADKAYIGDFDTLDVEPDRKLPLVTTKDILSGEVQWRGQGVINPFVEGGGLVELRSYPRLRRYLEAR
RDVIMDRHCARKSPDNWYRTIDRITPALASRPKLLIPDIKGEAHVVYEGGALYPHHNLYYVTSDEWELRALQAVLLSDVT
RLFMSTYSTRMRGGYLRFQAQYLRRIRIPQWANVSIMLRKALAEAAIMRDFPACNRAVFKLFELGNNERSALGGNGE

Specific function: This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease [H]

COG id: COG1002

COG function: function code V; Type II restriction enzyme, methylase subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 62352; Mature: 62352

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAAIEKLSSAAGTDSRGAIFTRVEVVDFILDLAGYTEDQLLHEKRLLEPSFGGGDFLL
CCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
PAIDRLLAAWKSSQHVTSVVEELGDAIRAVELHHKTFSSTRAAVIEKLRLESIDVQCAAT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LADRWLVHGDFLLSPLDGHFDFIVGNPPYVRQELIPAALLAEYRNRYQTMYDRADIYIPF
HHHHHEEECCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
IERSLSVLGKGGSLGFICADRWMKNRYGGPLRSLVANQFHLKIYVDMVDTPAFLSDVIAY
HHHHHHHHCCCCCEEEEEHHHHHHHCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHH
PAITIISRETPGATRIAHRPAIDRDTLANLADALCASNQLNEVDSISELAQVTNGAEPWL
CEEEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCE
LESSDQMALIRRLEKQFPSLEEVGCKVGIGVATGADKAYIGDFDTLDVEPDRKLPLVTTK
ECCCCHHHHHHHHHHHCCCHHHCCCEEECEEECCCCCCEECCCCCCCCCCCCCCCEEEEH
DILSGEVQWRGQGVINPFVEGGGLVELRSYPRLRRYLEARRDVIMDRHCARKSPDNWYRT
HHHCCCEEECCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IDRITPALASRPKLLIPDIKGEAHVVYEGGALYPHHNLYYVTSDEWELRALQAVLLSDVT
HHHHHHHHHCCCCEEEECCCCCEEEEEECCEECCCCCEEEEECCCHHHHHHHHHHHHHHH
RLFMSTYSTRMRGGYLRFQAQYLRRIRIPQWANVSIMLRKALAEAAIMRDFPACNRAVFK
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
LFELGNNERSALGGNGE
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVVAAIEKLSSAAGTDSRGAIFTRVEVVDFILDLAGYTEDQLLHEKRLLEPSFGGGDFLL
CCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
PAIDRLLAAWKSSQHVTSVVEELGDAIRAVELHHKTFSSTRAAVIEKLRLESIDVQCAAT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LADRWLVHGDFLLSPLDGHFDFIVGNPPYVRQELIPAALLAEYRNRYQTMYDRADIYIPF
HHHHHEEECCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
IERSLSVLGKGGSLGFICADRWMKNRYGGPLRSLVANQFHLKIYVDMVDTPAFLSDVIAY
HHHHHHHHCCCCCEEEEEHHHHHHHCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHH
PAITIISRETPGATRIAHRPAIDRDTLANLADALCASNQLNEVDSISELAQVTNGAEPWL
CEEEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCE
LESSDQMALIRRLEKQFPSLEEVGCKVGIGVATGADKAYIGDFDTLDVEPDRKLPLVTTK
ECCCCHHHHHHHHHHHCCCHHHCCCEEECEEECCCCCCEECCCCCCCCCCCCCCCEEEEH
DILSGEVQWRGQGVINPFVEGGGLVELRSYPRLRRYLEARRDVIMDRHCARKSPDNWYRT
HHHCCCEEECCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IDRITPALASRPKLLIPDIKGEAHVVYEGGALYPHHNLYYVTSDEWELRALQAVLLSDVT
HHHHHHHHHCCCCEEEECCCCCEEEEEECCEECCCCCEEEEECCCHHHHHHHHHHHHHHH
RLFMSTYSTRMRGGYLRFQAQYLRRIRIPQWANVSIMLRKALAEAAIMRDFPACNRAVFK
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
LFELGNNERSALGGNGE
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3001639; 7607512 [H]