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The map label for this gene is ispE

Identifier: 119357680

GI number: 119357680

Start: 2180274

End: 2181158

Strand: Reverse

Name: ispE

Synonym: Cpha266_1884

Alternate gene names: 119357680

Gene position: 2181158-2180274 (Counterclockwise)

Preceding gene: 119357683

Following gene: 119357677

Centisome position: 69.6

GC content: 50.73

Gene sequence:

>885_bases
ATGGATCATCTTTCAGTAAAATCATTTGCCAAAATCAATCTCGGCCTTCTCATAACCGGTAAACGCAAGGACGGCTATCA
TACGCTTGAGACGATTTTTGCGCCAATCAACTGGTATGATACCATCGGATTTTCCGATTCCGACGTGATTTCAATGAGCT
GCTCAAACATCGATCTTCCTGTTGACGACAATAATCTCTGCATCAGGGCGGCAAGAGCCTTGCAGCAGTCAGCCTCTTGT
TCGAAGGGCGCAGCCATGAATCTTCAGAAAGTGGTGCCTTTCGGTGCAGGGCTTGGCGGCGGGAGCAGCGATGCCGCAAC
GGTTCTCAGGGTACTCAATGAGCTGTGGAAGATTAATGTCTCTTCCGCCGAGCTGCATGAACTTGCCGTAAAACTCGGTG
CCGATGTACCTTATTTTCTTGAAATGAAGGGGCTGGCGTTTGCCAGAGGTATTGGTGACGAACTTGAGGATCTCGGTCTC
ACCCTGCCGTTTCATGTGGTGACCGTTTTTCCCGAAGAGCATATCTCAACGGTATGGGCCTATAAAAATTTCTATCAAAA
ATTCGACCGACCGGTTCCGGATCTCAGACTGCTTTTGCAGCGGCTTTGTCTGGATGGCGACCGTTCCGTTCTCGGGGCTT
TTGAAAATGACTTTGAACCGGCAGTGTTCGATCACTACCCGAAGGTTCGGGTTGTAAAAGAGAGTTTGCTCGATGCCGGC
AGTTTTTATGCCTCTCTTTCCGGGAGCGGTTCAGCGGTGTTCGGTCTGTTCGATACGCTGGAAAATGCAGCTGGCGCGGT
GTGCGCCATGCAGCAAAAGGGGTATCGGGTTACGCTTACCCCTCCCGGATTTTCCATGGAAGCACAGGCCGGGTCAAGAT
TATGA

Upstream 100 bases:

>100_bases
CTTGAGTATGGTTCGACAGCGTTTTGTTATACCAATCTGTTATGCTCCTTCATCATAACATAATATGACAAATTTTTTTC
TATAAAATCAATTGCGTTCT

Downstream 100 bases:

>100_bases
CCGTGATCGTTTGATACTCGCTTCCAGTTCGGCTGCAATAAGTCTGAGATCCTCGTCAAGAAAGTGAGAGGACTCAAGGC
GTGCCCGTTTAACAGCGGAA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDHLSVKSFAKINLGLLITGKRKDGYHTLETIFAPINWYDTIGFSDSDVISMSCSNIDLPVDDNNLCIRAARALQQSASC
SKGAAMNLQKVVPFGAGLGGGSSDAATVLRVLNELWKINVSSAELHELAVKLGADVPYFLEMKGLAFARGIGDELEDLGL
TLPFHVVTVFPEEHISTVWAYKNFYQKFDRPVPDLRLLLQRLCLDGDRSVLGAFENDFEPAVFDHYPKVRVVKESLLDAG
SFYASLSGSGSAVFGLFDTLENAAGAVCAMQQKGYRVTLTPPGFSMEAQAGSRL

Sequences:

>Translated_294_residues
MDHLSVKSFAKINLGLLITGKRKDGYHTLETIFAPINWYDTIGFSDSDVISMSCSNIDLPVDDNNLCIRAARALQQSASC
SKGAAMNLQKVVPFGAGLGGGSSDAATVLRVLNELWKINVSSAELHELAVKLGADVPYFLEMKGLAFARGIGDELEDLGL
TLPFHVVTVFPEEHISTVWAYKNFYQKFDRPVPDLRLLLQRLCLDGDRSVLGAFENDFEPAVFDHYPKVRVVKESLLDAG
SFYASLSGSGSAVFGLFDTLENAAGAVCAMQQKGYRVTLTPPGFSMEAQAGSRL
>Mature_294_residues
MDHLSVKSFAKINLGLLITGKRKDGYHTLETIFAPINWYDTIGFSDSDVISMSCSNIDLPVDDNNLCIRAARALQQSASC
SKGAAMNLQKVVPFGAGLGGGSSDAATVLRVLNELWKINVSSAELHELAVKLGADVPYFLEMKGLAFARGIGDELEDLGL
TLPFHVVTVFPEEHISTVWAYKNFYQKFDRPVPDLRLLLQRLCLDGDRSVLGAFENDFEPAVFDHYPKVRVVKESLLDAG
SFYASLSGSGSAVFGLFDTLENAAGAVCAMQQKGYRVTLTPPGFSMEAQAGSRL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=269, Percent_Identity=36.4312267657993, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CHLPD (A1BHM3)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912324.1
- ProteinModelPortal:   A1BHM3
- SMR:   A1BHM3
- STRING:   A1BHM3
- GeneID:   4571226
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1884
- NMPDR:   fig|290317.7.peg.1986
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14616
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31951; Mature: 31951

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLSVKSFAKINLGLLITGKRKDGYHTLETIFAPINWYDTIGFSDSDVISMSCSNIDLP
CCCCCCCHHHEEEEEEEEEECCCCCCHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCC
VDDNNLCIRAARALQQSASCSKGAAMNLQKVVPFGAGLGGGSSDAATVLRVLNELWKINV
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SSAELHELAVKLGADVPYFLEMKGLAFARGIGDELEDLGLTLPFHVVTVFPEEHISTVWA
CHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCHHHHHCCCCCCEEEEEECCHHHHHHHHH
YKNFYQKFDRPVPDLRLLLQRLCLDGDRSVLGAFENDFEPAVFDHYPKVRVVKESLLDAG
HHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCH
SFYASLSGSGSAVFGLFDTLENAAGAVCAMQQKGYRVTLTPPGFSMEAQAGSRL
HHEEECCCCCCEEHHHHHHHHHHHHHEEEHHCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MDHLSVKSFAKINLGLLITGKRKDGYHTLETIFAPINWYDTIGFSDSDVISMSCSNIDLP
CCCCCCCHHHEEEEEEEEEECCCCCCHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCC
VDDNNLCIRAARALQQSASCSKGAAMNLQKVVPFGAGLGGGSSDAATVLRVLNELWKINV
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
SSAELHELAVKLGADVPYFLEMKGLAFARGIGDELEDLGLTLPFHVVTVFPEEHISTVWA
CHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCHHHHHCCCCCCEEEEEECCHHHHHHHHH
YKNFYQKFDRPVPDLRLLLQRLCLDGDRSVLGAFENDFEPAVFDHYPKVRVVKESLLDAG
HHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHCCH
SFYASLSGSGSAVFGLFDTLENAAGAVCAMQQKGYRVTLTPPGFSMEAQAGSRL
HHEEECCCCCCEEHHHHHHHHHHHHHEEEHHCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA