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The map label for this gene is proS

Identifier: 119357673

GI number: 119357673

Start: 2174480

End: 2175925

Strand: Direct

Name: proS

Synonym: Cpha266_1877

Alternate gene names: 119357673

Gene position: 2174480-2175925 (Clockwise)

Preceding gene: 119357672

Following gene: 119357675

Centisome position: 69.39

GC content: 52.21

Gene sequence:

>1446_bases
GTGGCAGAAAAAATAACAACCCGCAGCGCAGACTACTCCCAGTGGTATATAGACCTTGTTCGATCCGCAAAACTTGCCGA
CTATTCCGATGTACGGGGATGCATGGTTATCAGACCAAACGGCTATGCCATCTGGGAAAAAATGCAAACCGCCCTTGACC
GGATGTTCAAGGAAACCGGCCACGTCAACGCCTATTTTCCGCTTTTCATCCCGGAAAGCTTTATTGCAAAAGAGGCTGAA
CACATAGAGGGGTTTGCTCCTGAATGCGCCGTAGTGACCCATGGCGGCGGCGAAGAGCTTGCTGAAAAACTCTATGTCAG
GCCAACCTCCGAAACCATTATCTGGTCGTCCTATAAAAAATGGATCCAGTCTTACCGCGACCTTCCGATACTCATCAACC
AGTGGGCCAATGTGGTGCGCTGGGAAATGCGAACCCGTCTCTTTCTGCGTACAACCGAATTTCTCTGGCAGGAAGGCCAC
ACGGCACATGCCACCCCTGAAGAGTCGCAGGAAGAGGTCGTGCGCATGATCAACGTCTACAAAACGTTTGCGGAAAATTA
TATGGCCCTGCCGGTTATCATCGGCAAAAAAACCGACAGCGAAAAATTTGCCGGTGCGGTTGATACCTGGTGCATCGAAG
CCATGATGCAGGATTCCAAAGCACTGCAGGCAGGAACGTCGCACAACCTCGGACAAAACTTCGCCAAAGCCTTTGACTGC
CAGTTCCAGACCCGTGATGGAAAACTCGACTATGTATGGGCGACAAGCTGGGGCGTTTCCACCCGTCTGATCGGCGCTCT
CATCATGGCACACTCCGATGACCGGGGCCTTGTGCTTCCGCCAAAACTCGCCACGCGCCAGGTTGTGATTATCCCGATTC
TCAAGGGCGACAAAGCCGCTGTATGTGAAAAAGCGCATGCCATATCGAGAACCCTCTCGGCAAACGGCATACCAGCCTTT
GTTGACGACAGCGAGCAGAACTCTCCCGGATGGAAGTTTGCAGAATACGAACTTCAGGGAATTCCTCTGCGCATCGAACT
CGGACCGAGGGACATCCAGAACGGCACCTGCATTGTTGCCAGACGCGATACCCTTGAAAAAACGGAACTTGCGCTTGACG
ATACCCTTTCGGTCTCGATCAGCGAAATCCTCAATGCGATTCAGCAGAACCTCTTCGATCGCGCTCTTGAGTTCCGTAAC
GAACACACCTTTGAGGCATCCAGTTACAGCGAGTTCAGGGAGATGGTTGAAAATGGCTTTGTTATCGCCCACTGGGACGG
CACGGCGGAAACGGAGGCTAAAATCAAGGAAGAGACAAAAGCAACCATACGAGTGCTTCCCGAAGAACCGGACTATATCT
CACGATACGCCATGCATGAAGCGGGAACCTGTATCTACTCCGGTAACCCCGCCGCACAAAAGGCAGTGTTTGCAAAAGCA
TACTGA

Upstream 100 bases:

>100_bases
CGCACCTTCGGGAGCTTATGGAAAAAATGAAAAAATTTGAAAAGTAGGTTGTATATTTCGAGCCCTTGATCTTCATGAAT
CGCACGAACCAATTCCTAAC

Downstream 100 bases:

>100_bases
GATGTTGCATAAAATTAATTCCGGCAACAACCCTCCAACAGGGGAGTTGCCGGAATTATCAAAAACCCGGTCAGCTCAGG
CATCCTTGAAGAACTCTTTG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAEKITTRSADYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQTALDRMFKETGHVNAYFPLFIPESFIAKEAE
HIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
TAHATPEESQEEVVRMINVYKTFAENYMALPVIIGKKTDSEKFAGAVDTWCIEAMMQDSKALQAGTSHNLGQNFAKAFDC
QFQTRDGKLDYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLATRQVVIIPILKGDKAAVCEKAHAISRTLSANGIPAF
VDDSEQNSPGWKFAEYELQGIPLRIELGPRDIQNGTCIVARRDTLEKTELALDDTLSVSISEILNAIQQNLFDRALEFRN
EHTFEASSYSEFREMVENGFVIAHWDGTAETEAKIKEETKATIRVLPEEPDYISRYAMHEAGTCIYSGNPAAQKAVFAKA
Y

Sequences:

>Translated_481_residues
MAEKITTRSADYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQTALDRMFKETGHVNAYFPLFIPESFIAKEAE
HIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGH
TAHATPEESQEEVVRMINVYKTFAENYMALPVIIGKKTDSEKFAGAVDTWCIEAMMQDSKALQAGTSHNLGQNFAKAFDC
QFQTRDGKLDYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLATRQVVIIPILKGDKAAVCEKAHAISRTLSANGIPAF
VDDSEQNSPGWKFAEYELQGIPLRIELGPRDIQNGTCIVARRDTLEKTELALDDTLSVSISEILNAIQQNLFDRALEFRN
EHTFEASSYSEFREMVENGFVIAHWDGTAETEAKIKEETKATIRVLPEEPDYISRYAMHEAGTCIYSGNPAAQKAVFAKA
Y
>Mature_480_residues
AEKITTRSADYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQTALDRMFKETGHVNAYFPLFIPESFIAKEAEH
IEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHT
AHATPEESQEEVVRMINVYKTFAENYMALPVIIGKKTDSEKFAGAVDTWCIEAMMQDSKALQAGTSHNLGQNFAKAFDCQ
FQTRDGKLDYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLATRQVVIIPILKGDKAAVCEKAHAISRTLSANGIPAFV
DDSEQNSPGWKFAEYELQGIPLRIELGPRDIQNGTCIVARRDTLEKTELALDDTLSVSISEILNAIQQNLFDRALEFRNE
HTFEASSYSEFREMVENGFVIAHWDGTAETEAKIKEETKATIRVLPEEPDYISRYAMHEAGTCIYSGNPAAQKAVFAKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=494, Percent_Identity=39.4736842105263, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI1786392, Length=165, Percent_Identity=30.3030303030303, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71991342, Length=446, Percent_Identity=39.9103139013453, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71991346, Length=353, Percent_Identity=44.1926345609065, Blast_Score=343, Evalue=9e-95,
Organism=Saccharomyces cerevisiae, GI6321808, Length=444, Percent_Identity=42.3423423423423, Blast_Score=383, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24649468, Length=475, Percent_Identity=38.7368421052632, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24649466, Length=475, Percent_Identity=38.7368421052632, Blast_Score=353, Evalue=1e-97,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_CHLPD (A1BHL6)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_912317.1
- ProteinModelPortal:   A1BHL6
- SMR:   A1BHL6
- STRING:   A1BHL6
- GeneID:   4571219
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1877
- NMPDR:   fig|290317.7.peg.1977
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54415; Mature: 54284

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKITTRSADYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQTALDRMFKETG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
HVNAYFPLFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKK
CCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHEECCCCCEEEHHHHHH
WIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHATPEESQEEVVRMINVY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KTFAENYMALPVIIGKKTDSEKFAGAVDTWCIEAMMQDSKALQAGTSHNLGQNFAKAFDC
HHHHCCCEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
QFQTRDGKLDYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLATRQVVIIPILKGDKAA
EEECCCCCEEEEEEECCCHHHHHHHHHHHEECCCCCEEECCCCCCCEEEEEEEECCCHHH
VCEKAHAISRTLSANGIPAFVDDSEQNSPGWKFAEYELQGIPLRIELGPRDIQNGTCIVA
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEE
RRDTLEKTELALDDTLSVSISEILNAIQQNLFDRALEFRNEHTFEASSYSEFREMVENGF
ECCCCHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCE
VIAHWDGTAETEAKIKEETKATIRVLPEEPDYISRYAMHEAGTCIYSGNPAAQKAVFAKA
EEEEECCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHCCC
Y
C
>Mature Secondary Structure 
AEKITTRSADYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQTALDRMFKETG
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
HVNAYFPLFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKK
CCEEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHEECCCCCEEEHHHHHH
WIQSYRDLPILINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHATPEESQEEVVRMINVY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KTFAENYMALPVIIGKKTDSEKFAGAVDTWCIEAMMQDSKALQAGTSHNLGQNFAKAFDC
HHHHCCCEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
QFQTRDGKLDYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLATRQVVIIPILKGDKAA
EEECCCCCEEEEEEECCCHHHHHHHHHHHEECCCCCEEECCCCCCCEEEEEEEECCCHHH
VCEKAHAISRTLSANGIPAFVDDSEQNSPGWKFAEYELQGIPLRIELGPRDIQNGTCIVA
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEE
RRDTLEKTELALDDTLSVSISEILNAIQQNLFDRALEFRNEHTFEASSYSEFREMVENGF
ECCCCHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCE
VIAHWDGTAETEAKIKEETKATIRVLPEEPDYISRYAMHEAGTCIYSGNPAAQKAVFAKA
EEEEECCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA