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The map label for this gene is ywkE [H]

Identifier: 119357671

GI number: 119357671

Start: 2172627

End: 2173532

Strand: Reverse

Name: ywkE [H]

Synonym: Cpha266_1875

Alternate gene names: 119357671

Gene position: 2173532-2172627 (Counterclockwise)

Preceding gene: 119357674

Following gene: 119357670

Centisome position: 69.36

GC content: 51.55

Gene sequence:

>906_bases
ATGATTGAACAGGGAAGAGGGACAAAAGAGTGGCAGGTTGTCGAACTGTTGAAAACCACCACGGCTTTTTTTGTGCAGAA
ACAGGTTGACGAGGCCCGGATCAGCGCAGAGCTTCTGCTCGCCTCTGTTCTCGGTCTTGACAGGCTTGGCCTCTATCTTA
ATCACAATCGCCCTGTTTATCCGGGCGAACTCGAAGCTTTCAGGGCACTTTGTCGTCAGCGTCTTGAGGGTAAGCCGGTG
CAGTATATAACCGGTGAACAGTTTTTTTACGGATTGCCTTTTTTTGTCGATAAGCGGGTTCTGATTCCACGTCCCGAAAC
AGAGCTGCTTGTTGAACACGCACTTGAGTTTCTTGGTCACGTTTCTGCAGCAGACGTTTCAGAAGCTGCTCTGCATCTGC
TGGATATTGGTACAGGGAGTGGCTGTATTGCTGTTACTCTTGCCAGCAGGTTGCCCTGCCTGATGGTTACAGCCATCGAT
ATTTCTACGGAAGCACTCGTTGTCGCCCGCAATAATGCTGAAAGGCATGGTGTTGCAGATCGGATACGTTTTCTGCATGC
CGACCTGTTCTCTCTCCCGGACGAAAGAGGGCTGTCTGCTCCTTTTGATGTTATTGTCTCCAATCCGCCGTACATTGCTG
AAGATGAGTGGGCTGGTCTGCAGCCGGAAGTCCGACTTTTCGAGCCACAGCTTGCGCTGACCACCAGAGATGGGATTGAG
TGCTATCATGCGGTGGCAGAAGTCGCGCCCTCTCTGTTGAAATCAGGAGGGATGCTCTGTTTTGAATCCCATGCTGATGC
GGCTTTGAAGGTTGCCGGGATCATGGAGCGTTGGGGGTTCTCATCGGTTGCGGTGATGAAAGATTACTCGGGACTTGACA
GGGTCGTTTCGGGAAAGATCGGTTGA

Upstream 100 bases:

>100_bases
GCTGTTGCAGTTGTTATCATCAGGGAAACTAACGAATTATTTTTCAAAGCGCCCGGTTATGGCTGATATTTTCCGGGTGT
GAAGTGAGCAGAAAGAAGGA

Downstream 100 bases:

>100_bases
TGAAATTATTAATCATCTTCGATTGTTTTTCACTGGGAGTTTCTGCAGGTTATTGCACAAGGTTCAAGGTTGTTTTCACC
TGGTTCATTTTTTTATTATT

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MIEQGRGTKEWQVVELLKTTTAFFVQKQVDEARISAELLLASVLGLDRLGLYLNHNRPVYPGELEAFRALCRQRLEGKPV
QYITGEQFFYGLPFFVDKRVLIPRPETELLVEHALEFLGHVSAADVSEAALHLLDIGTGSGCIAVTLASRLPCLMVTAID
ISTEALVVARNNAERHGVADRIRFLHADLFSLPDERGLSAPFDVIVSNPPYIAEDEWAGLQPEVRLFEPQLALTTRDGIE
CYHAVAEVAPSLLKSGGMLCFESHADAALKVAGIMERWGFSSVAVMKDYSGLDRVVSGKIG

Sequences:

>Translated_301_residues
MIEQGRGTKEWQVVELLKTTTAFFVQKQVDEARISAELLLASVLGLDRLGLYLNHNRPVYPGELEAFRALCRQRLEGKPV
QYITGEQFFYGLPFFVDKRVLIPRPETELLVEHALEFLGHVSAADVSEAALHLLDIGTGSGCIAVTLASRLPCLMVTAID
ISTEALVVARNNAERHGVADRIRFLHADLFSLPDERGLSAPFDVIVSNPPYIAEDEWAGLQPEVRLFEPQLALTTRDGIE
CYHAVAEVAPSLLKSGGMLCFESHADAALKVAGIMERWGFSSVAVMKDYSGLDRVVSGKIG
>Mature_301_residues
MIEQGRGTKEWQVVELLKTTTAFFVQKQVDEARISAELLLASVLGLDRLGLYLNHNRPVYPGELEAFRALCRQRLEGKPV
QYITGEQFFYGLPFFVDKRVLIPRPETELLVEHALEFLGHVSAADVSEAALHLLDIGTGSGCIAVTLASRLPCLMVTAID
ISTEALVVARNNAERHGVADRIRFLHADLFSLPDERGLSAPFDVIVSNPPYIAEDEWAGLQPEVRLFEPQLALTTRDGIE
CYHAVAEVAPSLLKSGGMLCFESHADAALKVAGIMERWGFSSVAVMKDYSGLDRVVSGKIG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=296, Percent_Identity=34.7972972972973, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1787463, Length=279, Percent_Identity=35.4838709677419, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87082085, Length=195, Percent_Identity=35.8974358974359, Blast_Score=107, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324266, Length=179, Percent_Identity=32.9608938547486, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24582226, Length=223, Percent_Identity=32.2869955156951, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 33090; Mature: 33090

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQGRGTKEWQVVELLKTTTAFFVQKQVDEARISAELLLASVLGLDRLGLYLNHNRPVY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCC
PGELEAFRALCRQRLEGKPVQYITGEQFFYGLPFFVDKRVLIPRPETELLVEHALEFLGH
CCHHHHHHHHHHHHHCCCCEEEECCCHHEECCCHHHCCEEECCCCHHHHHHHHHHHHHHC
VSAADVSEAALHLLDIGTGSGCIAVTLASRLPCLMVTAIDISTEALVVARNNAERHGVAD
CCCCHHHHHHHHHEECCCCCCCEEEEHHHCCCEEEEEEEECCCCEEEEECCCCCCCCHHH
RIRFLHADLFSLPDERGLSAPFDVIVSNPPYIAEDEWAGLQPEVRLFEPQLALTTRDGIE
HHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCCEEEEECCHHH
CYHAVAEVAPSLLKSGGMLCFESHADAALKVAGIMERWGFSSVAVMKDYSGLDRVVSGKI
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
G
C
>Mature Secondary Structure
MIEQGRGTKEWQVVELLKTTTAFFVQKQVDEARISAELLLASVLGLDRLGLYLNHNRPVY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCC
PGELEAFRALCRQRLEGKPVQYITGEQFFYGLPFFVDKRVLIPRPETELLVEHALEFLGH
CCHHHHHHHHHHHHHCCCCEEEECCCHHEECCCHHHCCEEECCCCHHHHHHHHHHHHHHC
VSAADVSEAALHLLDIGTGSGCIAVTLASRLPCLMVTAIDISTEALVVARNNAERHGVAD
CCCCHHHHHHHHHEECCCCCCCEEEEHHHCCCEEEEEEEECCCCEEEEECCCCCCCCHHH
RIRFLHADLFSLPDERGLSAPFDVIVSNPPYIAEDEWAGLQPEVRLFEPQLALTTRDGIE
HHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCCEEEEECCHHH
CYHAVAEVAPSLLKSGGMLCFESHADAALKVAGIMERWGFSSVAVMKDYSGLDRVVSGKI
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]