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The map label for this gene is mpl [H]

Identifier: 119357665

GI number: 119357665

Start: 2165936

End: 2167360

Strand: Direct

Name: mpl [H]

Synonym: Cpha266_1869

Alternate gene names: 119357665

Gene position: 2165936-2167360 (Clockwise)

Preceding gene: 119357663

Following gene: 119357666

Centisome position: 69.11

GC content: 50.25

Gene sequence:

>1425_bases
ATGAGCTCCATCTATTTTCTTGGAATCGGCGGTACGGCAATGGCTTCGGTTGCCGTCGCACTGTCGCATTCGGGACATGC
TGTCAGCGGTTCAGACACTCAGCTCTATCCGCCGATGAGCAACTACCTTGACACTCACAACATCCGATATTTCAGCGGTT
TTTCAGAAGAGAACATCCAAAAGGCATCCGCTGACCTTGTGGTTGCAGGCAATGCCATCAGCCGGGGAAACATAGAGCTT
GAATATGCGCTCAACAACCGCATGGAGCTTATCTCAATGCCAGACCTGGTACGAAGGGAGCTTATCGCTCAAAACACCTC
CCTTGTTGTAACCGGGACTCACGGCAAAACAACCACCACATCACTTCTTGCCTGGCTGCTTCAACATGGCGGTCTGAAAC
CGGGTTTTCTTGTTGGGGGAATACCTGAAAACTTTCCGGTGGCATGCCGGGCTTCAGGACGGAGTGAAAATGGTTTTTTT
GTCTCTGAAGGAGACGAATATGATACGGCGTTTTTCGACAAACGGAGCAAATTTCTTCTTTACAGGCCAGACGTTGTTAT
TATCAACAATATTGAGTTTGATCATGCCGATATTTTCAACTCTCTTGACGACATCAAACGAAGTTTCCGGCTTCTGGTCA
ACCTCATTCCCGGCAACGGGTTGCTGCTGGTGAACGGAGATGCTCCGGATGCGCTTGCCGTCGCGTCCCGCGCATTCTGC
CGGGTAGAACGATTCGGACTGAACGGCAACAGTGAATGGAGCGCCCGCAACATATCTCTTGAAAACAGTCTCACCGGTTT
TGATCTCTATTACAAGGGCAACCTTCAGGGCACCTGTCACGTTCCGCTTTTCGGATCCCATAATATCATGAACACCATTG
CCGCCGTTGCGGCGGCAATCCATTCAGGCATCTCTTTCGACAGCATTGCCGCAGGACTGCCGCTCTTCAAACGACCGAAA
CGTCGCATGGAAATAACCGATGACAGTGCGAACCGTATTACCCTGATCGAAGATTTCGCACACCACCCCACCGCTGTAAA
AGCAACACTTGAGGCTATAGCTGACCTCTACCCGAAACGCCGAATCATCGCCTGCTTCGAACCGCGATCGAACACAACAA
CACGCAATATCTTCCAGAATGAGCTGGCCGCCTGTTTCGATCCGGCTTCGATTGTCATCATGGGCATGGTTCACCGACCT
GAACGATACCGCCCGGAAGAGCGTCTCGACACCGAAAGACTGAAAAATGAACTGGAAAACAAGGGGAAGACCGTTTTTCT
TGCCGGAGAAAACCCCTCGATCTACCCGCAGGACATTATTGGATTCATAACAACGCGACTGCAACCGGAAGATGTGGTTG
TACTCCTGAGTAACGGGAGTTTCAGCAATCTGAAACAGGGACTATTCGATAGTTTAAAAAAATAA

Upstream 100 bases:

>100_bases
TACGCAGGGATACAGCGCAGCGATCGTCCCTGACAGTTGCGATCAAGCTGCTTTTTATGTATTTACTCTTGTTATTTCAT
AAAAAGAACTAAGAAATCCA

Downstream 100 bases:

>100_bases
TGCTCACGATTTTGATTTAGTTAGTTTATCTTCCAAAGTGTTTAAGGACATAAAACCGGTTACCATGATGCATAGTTCTG
CACAGGTAACCATAACCACA

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSIYFLGIGGTAMASVAVALSHSGHAVSGSDTQLYPPMSNYLDTHNIRYFSGFSEENIQKASADLVVAGNAISRGNIEL
EYALNNRMELISMPDLVRRELIAQNTSLVVTGTHGKTTTTSLLAWLLQHGGLKPGFLVGGIPENFPVACRASGRSENGFF
VSEGDEYDTAFFDKRSKFLLYRPDVVIINNIEFDHADIFNSLDDIKRSFRLLVNLIPGNGLLLVNGDAPDALAVASRAFC
RVERFGLNGNSEWSARNISLENSLTGFDLYYKGNLQGTCHVPLFGSHNIMNTIAAVAAAIHSGISFDSIAAGLPLFKRPK
RRMEITDDSANRITLIEDFAHHPTAVKATLEAIADLYPKRRIIACFEPRSNTTTRNIFQNELAACFDPASIVIMGMVHRP
ERYRPEERLDTERLKNELENKGKTVFLAGENPSIYPQDIIGFITTRLQPEDVVVLLSNGSFSNLKQGLFDSLKK

Sequences:

>Translated_474_residues
MSSIYFLGIGGTAMASVAVALSHSGHAVSGSDTQLYPPMSNYLDTHNIRYFSGFSEENIQKASADLVVAGNAISRGNIEL
EYALNNRMELISMPDLVRRELIAQNTSLVVTGTHGKTTTTSLLAWLLQHGGLKPGFLVGGIPENFPVACRASGRSENGFF
VSEGDEYDTAFFDKRSKFLLYRPDVVIINNIEFDHADIFNSLDDIKRSFRLLVNLIPGNGLLLVNGDAPDALAVASRAFC
RVERFGLNGNSEWSARNISLENSLTGFDLYYKGNLQGTCHVPLFGSHNIMNTIAAVAAAIHSGISFDSIAAGLPLFKRPK
RRMEITDDSANRITLIEDFAHHPTAVKATLEAIADLYPKRRIIACFEPRSNTTTRNIFQNELAACFDPASIVIMGMVHRP
ERYRPEERLDTERLKNELENKGKTVFLAGENPSIYPQDIIGFITTRLQPEDVVVLLSNGSFSNLKQGLFDSLKK
>Mature_473_residues
SSIYFLGIGGTAMASVAVALSHSGHAVSGSDTQLYPPMSNYLDTHNIRYFSGFSEENIQKASADLVVAGNAISRGNIELE
YALNNRMELISMPDLVRRELIAQNTSLVVTGTHGKTTTTSLLAWLLQHGGLKPGFLVGGIPENFPVACRASGRSENGFFV
SEGDEYDTAFFDKRSKFLLYRPDVVIINNIEFDHADIFNSLDDIKRSFRLLVNLIPGNGLLLVNGDAPDALAVASRAFCR
VERFGLNGNSEWSARNISLENSLTGFDLYYKGNLQGTCHVPLFGSHNIMNTIAAVAAAIHSGISFDSIAAGLPLFKRPKR
RMEITDDSANRITLIEDFAHHPTAVKATLEAIADLYPKRRIIACFEPRSNTTTRNIFQNELAACFDPASIVIMGMVHRPE
RYRPEERLDTERLKNELENKGKTVFLAGENPSIYPQDIIGFITTRLQPEDVVVLLSNGSFSNLKQGLFDSLKK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=477, Percent_Identity=35.8490566037736, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1786279, Length=432, Percent_Identity=27.3148148148148, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 52227; Mature: 52096

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIYFLGIGGTAMASVAVALSHSGHAVSGSDTQLYPPMSNYLDTHNIRYFSGFSEENIQ
CCEEEEEECCHHHHHHHHHHEECCCCEECCCCCEECCCHHHCCCCCCEEEECCCCHHHHH
KASADLVVAGNAISRGNIELEYALNNRMELISMPDLVRRELIAQNTSLVVTGTHGKTTTT
HCCCCEEEECCEECCCCEEEEEEECCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCHHH
SLLAWLLQHGGLKPGFLVGGIPENFPVACRASGRSENGFFVSEGDEYDTAFFDKRSKFLL
HHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCEEE
YRPDVVIINNIEFDHADIFNSLDDIKRSFRLLVNLIPGNGLLLVNGDAPDALAVASRAFC
ECCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHHH
RVERFGLNGNSEWSARNISLENSLTGFDLYYKGNLQGTCHVPLFGSHNIMNTIAAVAAAI
HHHEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHH
HSGISFDSIAAGLPLFKRPKRRMEITDDSANRITLIEDFAHHPTAVKATLEAIADLYPKR
HCCCCHHHHHHCCCHHHCCCCCEEECCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHCCCC
RIIACFEPRSNTTTRNIFQNELAACFDPASIVIMGMVHRPERYRPEERLDTERLKNELEN
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
KGKTVFLAGENPSIYPQDIIGFITTRLQPEDVVVLLSNGSFSNLKQGLFDSLKK
CCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSIYFLGIGGTAMASVAVALSHSGHAVSGSDTQLYPPMSNYLDTHNIRYFSGFSEENIQ
CEEEEEECCHHHHHHHHHHEECCCCEECCCCCEECCCHHHCCCCCCEEEECCCCHHHHH
KASADLVVAGNAISRGNIELEYALNNRMELISMPDLVRRELIAQNTSLVVTGTHGKTTTT
HCCCCEEEECCEECCCCEEEEEEECCCEEEECCHHHHHHHHHHCCCEEEEECCCCCCHHH
SLLAWLLQHGGLKPGFLVGGIPENFPVACRASGRSENGFFVSEGDEYDTAFFDKRSKFLL
HHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCEEE
YRPDVVIINNIEFDHADIFNSLDDIKRSFRLLVNLIPGNGLLLVNGDAPDALAVASRAFC
ECCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHHH
RVERFGLNGNSEWSARNISLENSLTGFDLYYKGNLQGTCHVPLFGSHNIMNTIAAVAAAI
HHHEECCCCCCCCCEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHH
HSGISFDSIAAGLPLFKRPKRRMEITDDSANRITLIEDFAHHPTAVKATLEAIADLYPKR
HCCCCHHHHHHCCCHHHCCCCCEEECCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHCCCC
RIIACFEPRSNTTTRNIFQNELAACFDPASIVIMGMVHRPERYRPEERLDTERLKNELEN
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
KGKTVFLAGENPSIYPQDIIGFITTRLQPEDVVVLLSNGSFSNLKQGLFDSLKK
CCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]