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The map label for this gene is mdeA [H]

Identifier: 119357564

GI number: 119357564

Start: 2008496

End: 2009788

Strand: Reverse

Name: mdeA [H]

Synonym: Cpha266_1767

Alternate gene names: 119357564

Gene position: 2009788-2008496 (Counterclockwise)

Preceding gene: 119357567

Following gene: 119357563

Centisome position: 64.13

GC content: 49.81

Gene sequence:

>1293_bases
ATGAGCAAGTTATCATCTTTATGCAGGTTTGAGACACTGCAGGTTCATGCAGGCCAGGAGCCTGATCCAACCACGAATGC
GCGGGCGGTGCCTATATATCAGACAACTTCCTATACGTTTGACAGTGTGGCGCACGGTTCCGATCTTTTTGCTCTCAAAG
CGTTTGGCAATATCTATACCAGGTTGATGAATCCGACTACCGATGTTCTCGAAAAGCGTGTTGCTGCGCTTGAAGGGGGA
GCGGCAGCGCTTGCGCTTGCAAGCGGACATTCGGCACAGTTTATTGCAATCACCAACCTCTGCCAGGCGGGAGACAATAT
CGTTTCGTCAAGTTATCTCTATGGAGGAACCTATAATCAGTTCAAGGTTACCTTTCCACGTCTCGGCATCAAGGTGAAGA
TTGTTGACGGTCAGAGTCCTGAAGCATTCCGGGCCGCGATTGATGAACAGACCAAAGCGCTCTACGTCGAATCTATCGGT
AACCCGGCATTTCATGTTCCCGATTTTGATGCTTTGGCTGAGTTGTCCCGTGAATATGGTATTCCGCTAATCGTAGACAA
TACGTTCGGGTGTGCAGGATATCTTGCTCGTCCGCTCGATCACGGCGCTTCGATTGTTGTTGAGTCAGCTACAAAATGGA
TAGGCGGTCATGGAACTTCAATGGGCGGCGTTATCGTTGATTCGGGAACGTTCAACTGGGGGAACGGAAAATTTCCGTTG
CTCAGTGAGCCCTCGGAAGGCTATCATGGACTTAAGTTTTATGAGACGTTCGGGTCTCTTGCCTTTATTATAAAGGCGAG
GGTTGAGGGTCTGCGGGATATCGGGCCAGCGATCAGTCCCTTTAACTCGTTTTTACTTCTGCAGGGGCTTGAGACGCTTT
CATTGCGTGTCCAGCGCCATGCCGACAATACGCTTGCGCTTGCCCGCTGGCTTGAGAAGCACCCTTCTGTAGCCTGGGTG
AACTATGCCGGACTTGAAGGGCATCAAACCTGGGAACTGGCAAAAAAATATCTTCAAAACGGATTTGGCTGTGTGCTAAC
CTTCGGTATCAGGGGCGGATATGAGAAAGCCGTTGGTTTTATCGAGAGCGTCAGGCTTGCAAGCCATCTTGCAAATGTAG
GCGATGCCAAGACTCTTGTTATCCATCCGGCATCAACAACGCATCAGCAGCTCAGTTCAGGCGAGCAGGAGTCTGCAGGT
GTCAGTAGCGATATGATCCGCGTATCGGTCGGGATAGAGCATATCGAGGACATCAAAGACGATTTTAAGCAAGCATTTAA
TAAAATTGGTTGA

Upstream 100 bases:

>100_bases
TTTGCATTTTCATACCGCTCTTTACCCCTTCGTTCTCGTTATTTCTCCGCTGATCTTTTCTCTCTTGACCTACAGCGTCA
ATCAATAATGAACGTTTGTT

Downstream 100 bases:

>100_bases
ACCGAATGAGAGACTACAGAGAGCTTATTTCAGATAGAACACTTTATTTTGCCTCCGATGTGCCGTTTCAGACCGAACTT
GGAGCTTTGTTACCAGAAGT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSKLSSLCRFETLQVHAGQEPDPTTNARAVPIYQTTSYTFDSVAHGSDLFALKAFGNIYTRLMNPTTDVLEKRVAALEGG
AAALALASGHSAQFIAITNLCQAGDNIVSSSYLYGGTYNQFKVTFPRLGIKVKIVDGQSPEAFRAAIDEQTKALYVESIG
NPAFHVPDFDALAELSREYGIPLIVDNTFGCAGYLARPLDHGASIVVESATKWIGGHGTSMGGVIVDSGTFNWGNGKFPL
LSEPSEGYHGLKFYETFGSLAFIIKARVEGLRDIGPAISPFNSFLLLQGLETLSLRVQRHADNTLALARWLEKHPSVAWV
NYAGLEGHQTWELAKKYLQNGFGCVLTFGIRGGYEKAVGFIESVRLASHLANVGDAKTLVIHPASTTHQQLSSGEQESAG
VSSDMIRVSVGIEHIEDIKDDFKQAFNKIG

Sequences:

>Translated_430_residues
MSKLSSLCRFETLQVHAGQEPDPTTNARAVPIYQTTSYTFDSVAHGSDLFALKAFGNIYTRLMNPTTDVLEKRVAALEGG
AAALALASGHSAQFIAITNLCQAGDNIVSSSYLYGGTYNQFKVTFPRLGIKVKIVDGQSPEAFRAAIDEQTKALYVESIG
NPAFHVPDFDALAELSREYGIPLIVDNTFGCAGYLARPLDHGASIVVESATKWIGGHGTSMGGVIVDSGTFNWGNGKFPL
LSEPSEGYHGLKFYETFGSLAFIIKARVEGLRDIGPAISPFNSFLLLQGLETLSLRVQRHADNTLALARWLEKHPSVAWV
NYAGLEGHQTWELAKKYLQNGFGCVLTFGIRGGYEKAVGFIESVRLASHLANVGDAKTLVIHPASTTHQQLSSGEQESAG
VSSDMIRVSVGIEHIEDIKDDFKQAFNKIG
>Mature_429_residues
SKLSSLCRFETLQVHAGQEPDPTTNARAVPIYQTTSYTFDSVAHGSDLFALKAFGNIYTRLMNPTTDVLEKRVAALEGGA
AALALASGHSAQFIAITNLCQAGDNIVSSSYLYGGTYNQFKVTFPRLGIKVKIVDGQSPEAFRAAIDEQTKALYVESIGN
PAFHVPDFDALAELSREYGIPLIVDNTFGCAGYLARPLDHGASIVVESATKWIGGHGTSMGGVIVDSGTFNWGNGKFPLL
SEPSEGYHGLKFYETFGSLAFIIKARVEGLRDIGPAISPFNSFLLLQGLETLSLRVQRHADNTLALARWLEKHPSVAWVN
YAGLEGHQTWELAKKYLQNGFGCVLTFGIRGGYEKAVGFIESVRLASHLANVGDAKTLVIHPASTTHQQLSSGEQESAGV
SSDMIRVSVGIEHIEDIKDDFKQAFNKIG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=33.5802469135802, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI299473759, Length=405, Percent_Identity=29.6296296296296, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25453487, Length=404, Percent_Identity=29.9504950495049, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790375, Length=415, Percent_Identity=30.1204819277108, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=23.4192037470726, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=436, Percent_Identity=30.2752293577982, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=415, Percent_Identity=30.8433734939759, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=415, Percent_Identity=31.3253012048193, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=55.6064073226545, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=31.4553990610329, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=26.86230248307, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=358, Percent_Identity=27.0949720670391, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=30.6413301662708, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46481; Mature: 46350

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLSSLCRFETLQVHAGQEPDPTTNARAVPIYQTTSYTFDSVAHGSDLFALKAFGNIYT
CCHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCEEEHHHHHHHHH
RLMNPTTDVLEKRVAALEGGAAALALASGHSAQFIAITNLCQAGDNIVSSSYLYGGTYNQ
HHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHCCCCHHCCCEEECCCCCE
FKVTFPRLGIKVKIVDGQSPEAFRAAIDEQTKALYVESIGNPAFHVPDFDALAELSREYG
EEEEECCCCEEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCC
IPLIVDNTFGCAGYLARPLDHGASIVVESATKWIGGHGTSMGGVIVDSGTFNWGNGKFPL
CEEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCEEEECCEEECCCCCCCC
LSEPSEGYHGLKFYETFGSLAFIIKARVEGLRDIGPAISPFNSFLLLQGLETLSLRVQRH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHC
ADNTLALARWLEKHPSVAWVNYAGLEGHQTWELAKKYLQNGFGCVLTFGIRGGYEKAVGF
CCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
IESVRLASHLANVGDAKTLVIHPASTTHQQLSSGEQESAGVSSDMIRVSVGIEHIEDIKD
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHCCCCCCEEEEEECHHHHHHHHH
DFKQAFNKIG
HHHHHHHHCC
>Mature Secondary Structure 
SKLSSLCRFETLQVHAGQEPDPTTNARAVPIYQTTSYTFDSVAHGSDLFALKAFGNIYT
CHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCEEEHHHHHHHHH
RLMNPTTDVLEKRVAALEGGAAALALASGHSAQFIAITNLCQAGDNIVSSSYLYGGTYNQ
HHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHCCCCHHCCCEEECCCCCE
FKVTFPRLGIKVKIVDGQSPEAFRAAIDEQTKALYVESIGNPAFHVPDFDALAELSREYG
EEEEECCCCEEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCC
IPLIVDNTFGCAGYLARPLDHGASIVVESATKWIGGHGTSMGGVIVDSGTFNWGNGKFPL
CEEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCEEEECCEEECCCCCCCC
LSEPSEGYHGLKFYETFGSLAFIIKARVEGLRDIGPAISPFNSFLLLQGLETLSLRVQRH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHC
ADNTLALARWLEKHPSVAWVNYAGLEGHQTWELAKKYLQNGFGCVLTFGIRGGYEKAVGF
CCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
IESVRLASHLANVGDAKTLVIHPASTTHQQLSSGEQESAGVSSDMIRVSVGIEHIEDIKD
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHHCCCCCCEEEEEECHHHHHHHHH
DFKQAFNKIG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]